Gut bacteria are essential for development of an invasive bark beetle by regulating glucose transport.

Insects and their gut bacteria form a tight and beneficial relationship, especially in utilization of host nutrients. The red turpentine beetle (RTB), a destructive and invasive pine pest, employs mutualistic microbes to facilitate its invasion success. However, the molecular mechanism underlying the utilization of nutrients remains unknown. In this study, we found that gut bacteria are crucial for the utilization of D-glucose, a main carbon source for RTB development. Downstream assays revealed that gut bacteria-induced gut hypoxia and the secretion of riboflavin are responsible for RTB development by regulating D-glucose transport via the activation of a hypoxia-induced transcription factor 1 (Hif-1α). Further functional investigations confirmed that Hif-1α mediates glucose transport by direct upregulation of two glucose transporters (ST10 and ST27), thereby promoting RTB development. Our findings reveal how gut bacteria regulate the development of RTB, and promote our understanding of the mutualistic relationship of animals and their gut bacteria.

Quantifying the direct and indirect impacts of urban waterlogging using input‒output analysis.

Increasingly frequent urban waterlogging disasters are having profound social and economic consequences. An appropriate and integrated evaluation of the total economic impacts of such disasters is crucial for achieving effective urban disaster risk management and sustainable development. However, existing metrics are inadequate for measuring the economic impacts of rainfall events of different intensities and their ripple effects across regions. Moreover, their ecological impacts have received insufficient attention. To address these gaps, we developed an integrated assessment framework for analyzing urban waterlogging losses and evaluating their various impacts. Taking Beijing as a case study, we used the InfoWorks ICM model to simulate urban waterlogging disaster risks, quantified direct economic losses, and assessed their environmental impacts. Additionally, we estimated indirect economic losses using input-output analysis and explored spillover effects. The results revealed increasing trends of direct economic losses and environmental losses corresponding to a longer return period. We observed synergies between these losses and their spatial heterogeneity. However, indirect impacts far outweighed direct impacts, with the former being 2.43 times larger than the latter. The cascading effect resulting from damage to infrastructure was also particularly pronounced. The industrial and spatial heterogeneity of interregional impacts was striking, with eastern provinces evidencing the most significant effects. By mapping the transmission paths of disaster losses along industrial chains and across regions, this study provides inputs that could assist policymakers in developing more effective measures for preventing and mitigating urban waterlogging disasters.

Determinants of the embodied CO2 transfers through electricity trade within China.

There is unequal spatial distribution of resource endowment, population density, industrial structure, and economic development with diverse differences in labor, energy, and capital productivities in China. However, previous studies paid little attention to the determinants of CO2 transfers embodied in electricity trade. In this study, we use both the absolute and comparative advantage theories to reveal the determinants of embodied CO2 transfers through electricity trade within China. Results show that China's electricity sector has higher labor productivity but lower asset efficiency and energy productivity than that of mining and manufacturing sectors. The large-scale electricity trade alleviates the shortage of electricity supply in developed regions by outsourcing to the less-developed regions, reduces the unequal spatial distribution of coal and natural gas reserves, and changes CO2 flow embodied in power grid. Econometric analysis shows that coal reserve contributes to the increase of embodied CO2 emission, while natural gas reduces the embodied CO2 emission. The regional differences in the opportunity cost of labor productivity of non-electricity sector are the dominant factor of the embodied CO2 transfers through electricity trade within China, while asset efficiency and energy productivity are not significant in the regressions. Our findings could provide details about China's power grid expansion when confronting climate mitigation in the future.

Prevalence of Azole-Resistant Aspergillus fumigatus is Highly Associated with Azole Fungicide Residues in the Fields.

Triazole resistance in Aspergillus fumigatus is a growing public health concern. In addition to its emergence in the therapy of invasive aspergillosis by triazole medicines, it has been frequently detected in agricultural fields all over the world. Here, we explore the potential link between residues of azole fungicides with similar chemical structure to triazole medicines in soil and the emergence of resistant A. fumigatus (RAF) through 855 500 km2 monitoring survey in Eastern China covering 6 provinces. In total, 67.3%, 15.2%, 12.3%, 2.9%, 1.5%, 0.4%, and 0.3% of the soil samples contained these five fungicides (tebuconazole, difenoconazole, propiconazole, hexaconazole, and prochloraz) of 0-100, 100-200, 200-400, 400-600, 600-800, 800-1000, and >1000 ng/g, respectively. The fractions of samples containing RAF isolates were 2.4%, 5.2%, 6.4%, 7.7%, 7.4%, 14.3%, and 20.0% of the samples with total azole fungicide residues of 0-100, 100-200, 200-400, 400-600, 600-800, 800-1000, and >1000 ng/g, respectively. We find that the prevalence of RAFs is positively (P < 0.0001) correlated with residual levels of azole fungicides in soils. Our results suggest that the use of azole fungicides in agriculture should be minimized and the intervals between treatments expanded to reduce the selective pressure toward the development of resistance in A. fumigatus in agricultural fields.

Spillover risk analysis of virtual water trade based on multi-regional input-output model -A case study.

Massive amounts of water embodied in commodities are transferred via interregional trade which increase the water scarcity risk of exporting region. This study proposed an integrated evaluation framework for sectoral physical water use risks and virtual water flow risks in Northeast China. The initial water use risks for different sectors by provinces were first assessed based on sectoral physical water consumption. Then based on the multi-regional input-output (MRIO) model, a virtual water trade network was established, and simultaneously the virtual scarce water in sectoral export of intermediate goods and final goods were accounted to investigate the virtual water flow risks by sectors. Finally, interprovincial embodied scarce water transfers between Northeast China and the rest of China were mapped, and by grafting the concept of 'spillover risk' to the virtual water trade, we analyzed the spillover risk difference of virtual water trade between regions. The results showed that the sectors of Agriculture (Ag) and Other manufacturing (OM) presented the highest risk of water use while Nonmetal mining (NmM) belonged to the potential high-risk sectors of water use for Northeast China. The sectors exported more virtual scarce water in intermediate goods also exported more in the final goods; and the sector of Manufacture of food products and tobacco processing (FP) was the largest contributor to the large exporting virtual scarce water for Liaoning and Jilin while Ag in Heilongjiang province was the largest exporter. The cumulative spillover risk index from rest of China to Liaoning province through virtual water trade is the highest; and the main risk spilt provinces to Northeast China were Xinjiang, Jiangsu, Anhui and Hebei province. The proposed risk framework for water utilization and trade may help promote the redistribution of water resources and explore pathways for sustainable management of water resources.

Biodegradation and detoxification of chlorimuron-ethyl by Enterobacter ludwigii sp. CE-1.

The application of the herbicide chlorimuron-ethyl has a lasting toxic effect on some succession crops. Here, a bacterium capable of utilizing chlorimuron-ethyl as the sole source of nitrogen was isolated from the contaminated soil and was identified as Enterobacter ludwigii sp. CE-1, and its detoxification and degradation of the herbicide were then examined. The biodegradation of chlorimuron-ethyl by the isolate CE-1 was significantly accelerated with increasing concentration (1-10mg/l) and temperature (20-40°C). The optimal pH for the degradation of chlorimuron-ethyl by the isolate CE-1 was pH 7.0. A pathway for the biodegradation of chlorimuron-ethyl by the isolate CE-1 was proposed, in which it could be first converted into 2-amino-4-chloro-6-methoxypyrimidine and an intermediate product by the cleavage of the sulfonylurea bridge and then transformed into saccharin via hydrolysis and amidation. The plant height and fresh weight of corn that had been incubated in nutrient solution containing 0.2mg/l of chlorimuron-ethyl significantly recovered to 83.9% and 83.1% compared with those in the uninoculated control, although the root growth inhibition of chlorimuron-ethyl could not be alleviated after inoculation for 14 d. The results indicate that the isolate CE-1 is a promising bacterial resource for the biodegradation and detoxification of chlorimuron-ethyl.

Assessing agricultural greenhouse gas emission mitigation by scaling up farm size: An empirical analysis based on rural household survey data.

Agriculture is a major contributor to greenhouse gas (GHG) emissions. Farm size affects agricultural production inputs and thus has impacts on agricultural GHG emissions. However, the effects and mechanisms behind this are still unclear. In this paper, we identified the effects and mechanisms of farm size on agricultural GHG emissions, based on survey data about over 20,000 rural households in China from 2009 to 2016. Firstly, we calculated the agricultural CO2, CH4, and N2O emissions using the life-cycle analysis (LCA). Secondly, the impacts of farm size on GHG emissions intensity were explored with a fixed effect model, based on the long-term rural household survey data. Finally, the mechanisms were tested by the mediation effect model. The results showed that a 1 % increase in farm size, on average, could reduce the GHG emissions intensity of rural households by 0.245 % from 2009 to 2016. The mechanism analysis showed that the larger farm size reduced GHG emissions intensity mainly by reducing the non-fixed input intensity and raising fixed input investment. By identifying the impacts and mechanisms of farm size on agricultural GHG emissions, this paper aims to provide insights for policymakers to achieve China's goal of reaching carbon neutrality by 2060.

G-Protein Coupled Receptor Gpr-1 Is Important for the Growth and Nutritional Metabolism of an Invasive Bark Beetle Symbiont Fungi Leptographium procerum.

Leptographium procerum has been demonstrated to play important roles in the invasive success of red turpentine beetle (RTB), one of the most destructive invasive pests in China. Our previous studies found that bacterial volatile ammonia plays an important role in the maintenance of the RTB-L. procerum invasive complex. In this study, we found a GPCR gene Gpr-1 that was a response to ammonia but not involved in the ammonia-induced carbohydrate metabolism. Deletion of Gpr-1 significantly inhibited the growth and pathogenicity but thickened the cell wall of L. procerum, resulting in more resistance to cell wall-perturbing agents. Further analyses suggested that Gpr-1 deletion caused growth defects that might be due to the dysregulation of the amino acid and lipid metabolisms. The thicker cell wall in the ΔGpr-1 mutant was induced through the cell wall remodeling process. Our results indicated that Gpr-1 is essential for the growth of L. procerum by regulating the nutritional metabolism, which can be further explored for potential applications in the management of RTB.

Mutation in cyp51A and high expression of efflux pump gene of Aspergillus fumigatus induced by propiconazole in liquid medium and soil.

Triazole resistance in Aspergillus fumigatus is a major cause of clinical inefficacy in the treatment of invasive aspergillosis (IA). The hypothesis that triazole fungicides have driven the development of resistance in A. fumigatus has garnered substantial attention due to the similar structure and global detection of antifungal resistant A. fumigatus (ARAF) isolates in the soil. However, there is little evidence linking the application of triazole fungicides to the emergence of ARAF in the soil. This study was conducted to test if the resistance in A. fumigatus and its associated mutations in cyp51A could be induced by propiconazole in liquid medium and soil. The results indicate that propiconazole can induce resistance by alteration of G138S in cyp51A, and the overexpression of cyp51A, AfuMDR3 and AfuMDR4. G138S in cyp51A was first detected in the soil and associated with resistance. The emergence of the ARAFs in the soil may depends upon the level of propiconazole, and the number of ARAFs in soil treated with propiconazole at 2- and 5-fold dose was much greater than those in soil treated at the recommended dosage. The current data indicate that propiconazole can induce triazole resistance in A. fumigatus and should be applied for agricultural purposes at levels at or below the recommended dosage to avoid the emergence of ARAF in the soil.

Microbial degradation of fomesafen and detoxification of fomesafen-contaminated soil by the newly isolated strain Bacillus sp. FE-1 via a proposed biochemical degradation pathway.

Fomesafen is a long residual herbicide and poses a potential risk to environmental safety, leading to an increasing need to find eco-friendly and cost-effective techniques to remediate fomesafen-contaminated soils. In this article, a novel strain of Bacillus sp., FE-1 was isolated from paddy field soil. This strain was found to degrade fomesafen both in liquid medium and in soil. >82.9% of fomesafen, at concentrations of 0.5, 1 and 10mgL-1, was degraded by Bacillus sp. FE-1 in liquid medium within 14h. The optimal pH and temperature for degradation were 7.0 and 35°C, respectively. Soil samples inoculated with strain FE-1 showed significantly increased rates of fomesafen degradation. Two metabolites of fomesafen degradation were detected and identified as amino-fomesafen and 5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-amino-benzoic acid. This is the first report of a novel fomesafen biodegradation pathway involving the reduction of a nitro group followed by the hydrolysis of an amide bond. The excellent remediation capability of the isolate FE-1 to detoxify fomesafen-contaminated soil was shown by bioassay of the sensitive aftercrop corn. The results indicate that Bacillus sp. FE-1 has potential for use in the bioremediation of fomesafen-contaminated soil.