Argpyrimidine bonded to RAGE regulates autophagy and cell cycle to cause periodontal destruction.

Argpyrimidine (APMD), a methylglyoxal-arginine-derived product, is one of the main products of diabetes mellitus. We aimed to systematically investigate the role of APMD in regulating autophagy activity, with a specific focus on the finding of APDM binding molecule, matching amino acid residues, autophagy flux and proteins, cell cycle arrest, cell skeleton and migration, PI3K/AKT/mTOR pathways, inflammatory signals, alveolar bone destruction, and inhibition verification. In this study, binding to 59/94/121 amino acid residues of advanced glycosylation end product receptor (RAGE), APMD suppressed PI3K/AKT/mTOR pathway to attenuate cell survival of periodontal ligament cells (PDLCs). Simultaneously, autophagy proteins ATG5, Beclin1, and LC3-II/I expression ratio were upregulated while P62/SQSTM was downregulated. Cell cycle arrested at G0/G1 with enhancing Cyclin D1/CDK4 and decreasing Cyclin A/CDK2 expression. Inhibition of autophagy abrogated APMD-induced cell cycle arrest. Furthermore, the inflammation regulation network of matrix metalloproteinase (MMP)-2, MMP-9, MAPKs and NF-κB pathways were activated by APMD. Rat periodontal models confirmed that APMD induced alveolar bone resorption, increased inflammatory infiltrates, and degraded collagen fibers through RAGE and PI3K. APMD-induced autophagy, G0/G1 arrest, pro-inflammatory signals activating and periodontal destruction were reversed by RAGE knockdown while aggravated by PI3K inhibitor. This study provides the first evidence that APMD bind to RAGE to regulate autophagy and cell cycle of PDLCs through the PI3K/AKT/mTOR pathway, thereby promoting periodontal destruction.

The nudging effect of AIGC labeling on users' perceptions of automated news: evidence from EEG.

Introduction: In the context of generative AI intervention in news production, this study primarily focuses on the impact of AI-generated content (AIGC) labeling cues on users' perceptions of automated news based on nudge theory. Methods: A 2 (authorship disclosure nudge cues: with vs. without AIGC label) × 2 (automated news type: descriptive vs. evaluative news) within-subject experiment was carried out. Thirty-two participants were recruited to read automated news, evaluate the perceived content trustworthiness, and record with an EEG device. Results: The results demonstrated that disclosure of AIGC labeling significantly reduced the trustworthiness perception of both fact-based descriptive and opinion-based evaluative news. In EEG, the delta PSD, theta PSD, alpha PSD, and beta PSD with disclosure of AIGC labeling were significantly higher than those without AIGC labeling. Meanwhile, in descriptive news conditions, TAR with AIGC labeling was higher than without AIGC labeling. Discussion: These results suggested that AIGC labeling significantly improves the degree of attention concentration in reading and deepens the degree of cognitive processing. Users are nudged by AIGC labeling to shift their limited attention and cognitive resources to re-evaluate the information quality to obtain more prudent judgment results. This helps to supplement the theoretical perspective on transparent disclosure nudging in the Internet content governance research field, and it can offer practical guidance to use content labeling to regulate the media industry landscape in the face of AI's pervasive presence.

A new subgingival sling suture technique for periodontally accelerated osteogenic orthodontics.

This study aimed to design a modified subgingival sling suture for periodontally accelerated osteogenic orthodontics (PAOO) as well as evaluate postoperative effects including gingival recession (GR), alveolar bone crest resorption, dental plague accumulation on sutures and alveolar bone augmentation. Twelve patients with bone defects in the anterior alveolar region of the mandible were included in this study. Subgingival sling suture, developed from traditional sling suture, was applied in modified PAOO operation. Probing depth, bleeding index, and GR were assessed, and cone-beam computerized tomography and laser microscope for thread surface were evaluated at baseline, postoperative 1 and 3 months to analyze the effects. Alveolar bone thickness on the labial side at the midpoint of the middle third of the root increased from 0.96 ±â€…0.28 mm to 3.38 ±â€…0.61 mm (P < .01), and that of the apical third advanced from 1.26 ±â€…0.33 mm to 3.61 ±â€…1.02 mm (P < .01), both exhibiting significant increase. No significant alveolar bone crest loss, probing depth increase, GR, and attachment loss was observed. This modified PAOO operation, associated with novel subgingival sling suture, productively augments alveolar bone volume and addresses problems in terms of GR and vertical loss of alveolar bone.