RESUMO
The polymorphisms of DGAT2 gene in 92 Jiaxian red cattle were analyzed using PCR-RFLP method. Results showed that the PCR products digested with TaqI demonstrated polymorphisms, and showed two kinds of genotype: AA and AB. This polymorphic locus of DGAT2 gene was at Hardy-Weinberg equilibrium (P>0.05). The results of general linear model analysis showed that polymorphisms of DGAT2 gene had significant association (Plt;0.05) with body weight and body length, while had no significant association with others growth traits (P>0.05). Therefore, genotype AB may have positive effect on body weight.
Assuntos
Diacilglicerol O-Aciltransferase/genética , Polimorfismo Genético/genética , Animais , Tamanho Corporal/genética , Peso Corporal/genética , Bovinos , Genótipo , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição/genéticaRESUMO
The associations between SNPs in intron 6 and intron 7 of the bovine diacylglycerolacyltransferase (DGAT2) gene and growth traits in purebred Nanyang cattle (n=131) were reported in this study. Alleles detected in intron 6 were allele A and B with frequencies of 0.875 and 0.125, respectively; alleles detected in intron 7 were allele N and M with frequencies of 0.968 and 0.032, respectively. The animals with genotype AA in intron 6 showed 3.8% increases in body height (P < 0.01), 3.9% increases in body weight (P < 0.05), 3.8% (P < 0.05), 3.6% (P < 0.05), 3.8% (P < 0.01) and 3.1%( P < 0.01) increase in body length at age six-month to two-year compared with genotype AB, respectively. They also showed 3.8 % (P < 0.05), 3.4 % ( P < 0.01), 3.7% (P < 0.01) and 4.3% (P < 0.01) increases in heart girth at age six-month to two-year respectively. Animals with the NM genotype show significantly higher average heart girth at age two years (P < 0.05), and Hucklebone width (P < 0.01) at age eighteen-month (P < 0.01) and two-year (P < 0.01) compared with animals with the genotype NN, respectively.
Assuntos
Bovinos/genética , Diacilglicerol O-Aciltransferase/genética , Polimorfismo Genético/genética , Alelos , Animais , Peso Corporal/genética , Genótipo , Íntrons/genética , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de RestriçãoRESUMO
PCR-RFLP was applied to analyze the effect of the genetic variations of the POU1F1 gene on growth traits of 100 Nanyang cattle. The results showed that the 451 bp PCR product digested with Hinf I demonstrated polymorphism in the population, which was at Hardy-Weinberg equilibrium. Moreover, the frequencies of alleles A/B in the Nanyang population were 0.465/0.535. The association of the variations of the POU1F1 gene with the growth traits in the population was analyzed. The following parameters were greater in individuals with a genotype BB than in those with an genotype AB: birth weight, average weight increase before ablactation, body height at 12 months, body weight, body length, and chest girth at 6 months and 12 months (P<0.05). The body weight at 12 months was higher in the BB individuals than in the AA individuals (P <0.05). The body weight and body sizes also showed a trend of allele B> allele A in the other age groups. Therefore, the genotype BB maybe a dominant genotype and the allele B may be a dominant allele. These results imply that the allele B of the POU1F1 gene is likely to positively affect the growth traits.