RESUMO
OBJECTIVE: To To investigate the changes of MicroRNA-134, CREB and p-CREB expression in epileptic rat brains in order to elucidate the molecular mechanisms of epilepsy, providing new ideas for clinical treatment. METHODS: Sixty-four Spraque-Dawley (SD) rats were divided into groups randomly, including control group, six hours after seizure group, 24-hour group, three-day group, one-week group, two-week group, four-week group, and eight-week group. All groups were placed under a pilocarpine-induced epilepsy model except the control group, and all rats were decapitated in different points of time. Brain specimens were taken for quantitative PCR experiments, immunohistochemistry and Western blot experiments. The results of the epilepsy model groups and the control group were compared. RESULTS: There were no significant differences between the six hours after seizure group, the 24-hour group and the control group about the MicroRNA-134 levels. MicroRNA-134 in the hippocampus tissue of the three-day group significantly reduced compared with the control group; same result was observed with the one-week, two-week, four-week and eight-week groups. The CREB and p-CREB levels in the three-day group's rat hippocampus significantly increased compared with the control group; and the high levels of CREB and p-CREB were constantly maintained in the one-week, two-week, four-week and eight-week groups. CONCLUSIONS: The MicroRNA-134 level of the epileptic rat hippocampus is significantly lower than normal after three days, and continues to maintain a low level; while CREB and p-CREB levels are rsignificantly increased after three days, and continue to remain at a high level. MicroRNA-134 plays a role in inhibiting synaptic plasticity by inhibiting CREB and p-CREB expressions.
RESUMO
Objective@#To understand the epidemiologic characteristics of injury among children aged 0-5 years in 2018, and to provide evidence for prevention and control measures of injury in children.@*Methods@#Data of injury in children aged 0-5 years in China in 2018 were collected from National Injury Surveillance System (NISS) for the descriptive epidemiologic analysis.@*Results@#A total of 74 355 injury cases were reported in children aged 0-5 years in 2018, with the gender ratio of 0.66, in which 98.13% were unintentional injuries. The top three causes of injury were falls (56.76%), blunt injuries (9.80%) and animal related injuries (7.81%). Injuries mainly occurred during 17:00—20:59 (32.09%) and 10:00—11:59 (13.95%). The top three places where injuries might occur were home (65.70%), public residence (13.06%), highway/street (10.94%). Recreational activities (64.21%), basic life activities (16.93%) and walking (5.66%) were the main activities when injuries occurred. Of all injury cases, most were mild, while 13.73% of cases were moderate or severe. Head, upper limb and lower limb were main injuries sites, which accounted for 49.44%, 28.59% and 11.70% of all cases respectively.@*Conclusion@#Injury is an important cause for children aged 0-5 years to go to the outpatient/emergency department for treatment, indicating that the prevention of injury in such age group should be strengthened. It is recommended to further improve the data collection on child injury in order to provide more evidence for child injury intervention.