RESUMO
A phorbol ester promoting agent, 12-O-tetradecanoylphorbol-13-acetate, enhances plasminogen activator production in hamster cell lines that synthesize plasminogen activator immunologically identical with plasminogen activator of normal hamster lung cells. 12-O-Tetradecanoylphorbol-13-acetate exhibits a high degree of specificity in the type of plasminogen activator evoked, which is always of the lung form. No enhancement of plasminogen activator production was detected in hamster cell lines synthesizing plasminogen activator of different antigenic form, although in the presence of 12-O-tetradecanoylphorbol-13-acetate, cells of one such line initiated synthesis of plasminogen activator of the lung form.
Assuntos
Transformação Celular Neoplásica , Forbóis/farmacologia , Ativadores de Plasminogênio/biossíntese , Acetato de Tetradecanoilforbol/farmacologia , Animais , Anticorpos/administração & dosagem , Cricetinae , Epitopos , Imunoglobulina G , Pulmão/metabolismo , Especificidade de Órgãos , Ativadores de Plasminogênio/imunologia , Vírus 40 dos SímiosRESUMO
The analysis of 6-mercaptopurine (6-MP) by a rapid, sensitive, and specific high performance liquid chromatographic assay is described. This method does not require any derivatization and allows for direct quantitation of the drug. Only 200 microliters of serum or plasma is needed, and concentrations as low as 10 ng/ml can be reliably quantitated. Analytical recoveries of 83% for 6-MP and 71% for the internal standard, 6-thioguanine, are obtained. Between-day precision studies at three plasma concentrations of 6-MP produce a mean coefficient of variation of 7.2%. This assay will be clinically useful for pharmacokinetic studies of 6-MP in patients in remission of acute lymphoblastic leukemia.