RESUMO
Huntington disease (HD) is an adult-onset, autosomal dominant inherited human neurodegenerative disorder characterized by hyperkinetic involuntary movements, including motor restlessness and chorea, slowing of voluntary movements and cognitive impairment. Selective regional neuron loss and gliosis in striatum, cerebral cortex, thalamus, subthalamus and hippocampus are well recognized as neuropathological correlates for the clinical manifestations of HD. The underlying genetic mutation is the expansion of CAG trinucleotide repeats (coding for polyglutamines) to 36-121 copies in exon 1 of the HD gene. The HD mRNA and protein product (huntingtin) show widespread distribution, and thus much remains to be understood about the selective and progressive neurodegeneration in HD. To create an experimental animal model for HD, transgenic mice were generated showing widespread expression of full-length human HD cDNA with either 16, 48 or 89 CAG repeats. Only mice with 48 or 89 CAG repeats manifested progressive behavioural and motor dysfunction with neuron loss and gliosis in striatum, cerebral cortex, thalamus and hippocampus. These animals represent clinically relevant models for HD pathogenesis, and may provide insights into the underlying pathophysiological mechanisms of other triplet repeat disorders.
Assuntos
Comportamento Animal , Doença de Huntington/genética , Proteínas do Tecido Nervoso/genética , Neurônios/patologia , Proteínas Nucleares/genética , Animais , Apoptose , Corpo Estriado/química , DNA Complementar/biossíntese , DNA Complementar/química , Humanos , Proteína Huntingtina , Doença de Huntington/patologia , Camundongos , Camundongos Transgênicos , Proteínas do Tecido Nervoso/análise , Proteínas do Tecido Nervoso/biossíntese , Proteínas Nucleares/análise , Proteínas Nucleares/biossíntese , Mapeamento por Restrição , Repetições de Trinucleotídeos/genética , Ubiquitinas/análiseRESUMO
Highly differentiated cultures of rat and mouse sensory ganglia were treated for varying periods (up to 6 hr) with selected doses (1 x 10(-3)M to 5 x 10(-5)M) of the cardiac glycoside, ouabain (Strophanthin G). This inhibitor of active Na and K transport produced a selective and progressive swelling of elements of the Golgi complex in many, but not all, neurons. After 6 hr of treatment, virtually all Golgi complexes in affected neurons were either altered or absent; large cytoplasmic vacuoles limited by agranular membrane were prominent in these neurons. This response was not observed in satellite cells and Schwann cells. Within a few hours of ouabain withdrawal, the neuronal vacuoles disappeared and normal Golgi areas were again observed. These observations suggest that there is a site for active transport of Na and K on the Golgi membrane of these neurons. In discussing the possible significance of this observation, it is suggested that if this site were directed so that cation was actively pumped from Golgi cisternae into cytoplasm (and if there were differential water and ion permeabilities in various parts of the endoplasmic reticulum-Golgi system), then such a pumping mechanism could provide an explanation for the concentrating function of the Golgi apparatus.
Assuntos
Complexo de Golgi/efeitos dos fármacos , Condução Nervosa/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Ouabaína/farmacologia , Sódio/metabolismo , Animais , Transporte Biológico Ativo/efeitos dos fármacos , Técnicas de Cultura , Eletrofisiologia , Íons , Potenciais da Membrana , Camundongos , Microscopia Eletrônica , Potássio/metabolismo , RatosRESUMO
A current hypothesis links the neuroexcitatory properties of certain acidic amino acids to their ability to cause selective neuronal lesions. Intracerebral injection of the neuroexcitatory tryptophan metabolite, quinolinic acid, has behavioral, neurochemical, and neuropathological consequences reminiscent of those of exogenous excitotoxins, such as kainic and ibotenic acids. Its qualities as a neurotoxic agent suggest that quinolinic acid should be considered as a possible pathogenic factor in neurodegenerative disorders.
Assuntos
Encéfalo/efeitos dos fármacos , Piridinas/farmacologia , Ácidos Quinolínicos/farmacologia , Animais , Axônios/efeitos dos fármacos , Encéfalo/enzimologia , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/enzimologia , Corpo Estriado/ultraestrutura , Hipocampo/efeitos dos fármacos , Neuroglia/efeitos dos fármacos , Neurônios/efeitos dos fármacos , RatosRESUMO
Well-myelinated cultures of mouse dorsal root ganglia incubated for 48 h with sigma-aminolevulinic acid (ALA) showed intense porphyrin fluorescence localized in myelin sheaths but not in axons or neuronal somata. When the cultures were continuously incubated with a high concentration of lead, focal swelling and segmental degeneration of myelin began to develop within 2 wk. Incubation of cultures with ALA after 3 wk of lead treatment revealed markedly decreased porphyrin fluorescence in myelin sheaths compared with untreated controls. After 6 wk of lead treatment, myelin showed severe segmental degeneration. Porphyrin fluorescence from ALA at this time was barely detectable in these cultures. No fluorescence was visible in the demyelinated axons; however, silver-impregnation staining after fixation demonstrated continuity of the axon despite the severe loss of myelin. When cultures were continuously incubated with lead and heme together for 6 wk, the segmental demyelination seen in cultures treated with lead alone did not occur. These findings suggest that the lead-induced segmental demyelination in cultured mouse dorsal root ganglia may be due to toxic effects of the metal on the heme biosynthetic pathway in myelinating cells and that exogenous heme may counteract this toxic effect of lead.
Assuntos
Gânglios Espinais/metabolismo , Heme/farmacologia , Intoxicação por Chumbo/metabolismo , Chumbo/farmacologia , Bainha de Mielina/metabolismo , Porfirinas/biossíntese , Ácido Aminolevulínico/farmacologia , Animais , Axônios/metabolismo , Feto , Gânglios Espinais/efeitos dos fármacos , Heme/biossíntese , Camundongos , Bainha de Mielina/efeitos dos fármacos , Neurônios/metabolismo , Técnicas de Cultura de ÓrgãosRESUMO
Mature organized cultures of mouse dorsal root ganglion (MDRG) were infected with herpes simplex virus, type 1 (HSV 1) and type 2 (HSV 2). Onset of infectious virus production occurred faster and reached higher levels in HSV 2-infected cultures. Neurons, supporting cells and myelin were affected in both types of infection, but morphological changes occurred significantly earlier and more dramatically with the type 2 infection. The pattern of myelin changes was distinctly different in the two types of infection. Within 20 hours post infection nerve cells infected with HSV 2 developed several types of intranuclear inclusions consisting of membranes and filaments; no such neuronal inclusions were seen with HSV 1 infection. HSV 2 infection showed frequent, large, membranous inclusions in supporting cell nuclei whereas, only rare, small inclusions of this type were seen in supporting cells infected with HSV 1. The observations demonstrate that the two virus types produced different virus replication patterns and different morphologic changes in long term cultures of MDRG. There appears to be a differential response of neurons and non-neuronal elements to the virus in this tissue substrate. Viral latency was not induced in this system by direct inoculation of the virus under the conditions described.
Assuntos
Gânglios Espinais/microbiologia , Simplexvirus/crescimento & desenvolvimento , Animais , Gânglios Espinais/patologia , Infecções por Herpesviridae/patologia , Camundongos , Bainha de Mielina/patologia , Neurônios/patologia , Técnicas de Cultura de Órgãos , Fatores de Tempo , Replicação ViralRESUMO
Living, mature organotypic cultures of chick dorsal root ganglion maintained in culture for 3 weeks were incubated in medium containing various levels of a precursor of porphyrin and heme formation, delta-aminolevulinic acid (ALA), (0.5 mM to 10 mM) or a combination of ALA (10 MM) and a metal chelator, CaMg-EDTA (5 mM) for up to 48 hours. Although no morphologic changes occurred in the cultures incubated with these compounds as observed by bright-field or dark-field microscopy, fluorescence microscopic study at 12, 24, and 48 hours demonstrated an intense red fluorescence with in the non-neuronal cells of the cultures (Schwann cells, fibroblasts, and macrophages) but not in the nerve cells. Spectrofluorometric analysis of perchloric acid-methanol extracts of the cultures revealed an emission spectrum characteristic of porphyrins. Autoradiographic studies, using 14C-labelled ALA, indicated that ALA was taken up by all cells (nerve cells as well as non-neuronal cells) in the cultures. The cultures incubated with ALA plus the metal chelator CaMg-EDTA showed the same distribution of porphyrin fluorescence, but a 2-fold increase in the amount of porphyrins was generated, when compared to cultures incubated with ALA alone. This observation suggests that a considerable fraction of porphyrins may be utilized to form heme in these cells since CaMa-EDTA blocks ferrochelatase activity, the terminal enzyme in the heme biosynthetic pathway. This is the first demonstration of active porphyrin-heme biosynthesis from ALA in cultured nervous system cells. Our results indicate that this biosynthetic pathway remains active in 3-week old cultures of chick dorsal root ganglion, and further, that the pathway appears to be predominantly present in the non-neuronal cellular elements of the ganglion rather than in nerve cells.
Assuntos
Ácido Aminolevulínico/metabolismo , Gânglios Espinais/metabolismo , Heme/biossíntese , Ácidos Levulínicos/metabolismo , Animais , Autorradiografia , Embrião de Galinha , Gânglios Espinais/citologia , Neurônios/metabolismo , Técnicas de Cultura de Órgãos , Especificidade de Órgãos , Porfirinas/metabolismo , Espectrometria de FluorescênciaRESUMO
Organotypic cultures of mouse spinal cord with attached dorsal root ganglia, which contain both central and peripheral myelin in the one unit of tissue, were infected with HSV 1 or HSV 2 and studied using electron microscopy. Intranuclear viral nucleocapsids and intracytoplasmic enveloped particles were found in the Schwann cells associated with peripheral myelin and in oligodendroglia associated with central myelin. Degeneration of peripheral myelin most commonly involved an asymmetrical swelling of the myelin lamellae, whereas degeneration of central myelin was characterized by a more generalized swelling resulting in separation of the myelin lamellae. Degeneration of both central and peripheral myelin was found in the presence of intact axons which were indistinguishable from those in controls.
Assuntos
Gânglios Espinais/microbiologia , Herpes Simples/patologia , Medula Espinal/microbiologia , Animais , Técnicas de Cultura , Efeito Citopatogênico Viral , Gânglios Espinais/patologia , Cobaias , Proteínas da Mielina/metabolismo , Bainha de Mielina/microbiologia , Bainha de Mielina/ultraestrutura , Degeneração Neural , Células de Schwann/microbiologia , Células de Schwann/ultraestrutura , Sorotipagem , Simplexvirus/ultraestrutura , Medula Espinal/patologia , Replicação ViralRESUMO
Alzheimer's disease (AD) is characterized neuropathologically by the presence of neuritic plaques (NP) in cerebral cortex and hippocampus, as well as intraneuronal neurofibrillary tangles and granulovacuolar degeneration. The etiology of plaque formation has remained obscure, but morphologically NP are known to contain amyloid cores surrounded by astrocytes and degenerating neurons. Although growth factors are important in growth, differentiation and regrowth in response to injury, studies relating growth factors to AD have been lacking. Epidermal growth factor (EGF) plays an important role outside the central nervous system (CNS) through interaction with its specific receptor, EGF-R. Using an antibody to EGF-R (three-step immunoperoxidase staining) in conjunction with fluorescence staining, we found that the majority of NP from patients with pathologically confirmed AD as well as those few NP in the normal aging brain showed intense EGF-R immunoreactivity. Specific staining was seen at the periphery of plaques but not in the central amyloid core. Tissue sections from AD cases were also reacted with antibodies to both glial fibrillary acidic protein (GFAP) and paired helical filaments (PHF) in an attempt to identify which component of the NP was reactive for EGF-R. The antibody to PHF densely stained the periphery of NP but not the central core in a majority of NP. The antibody to GFAP stained a few reactive astrocytes that bordered plaques in only a small proportion of all plaques present. We conclude that the neuron and its processes although not exclusively may be the site of EGF-R immunoreactivity. An EGF/EGF-R system within the CNS may play an important part in scar formation in response to neuronal injury and death or it may function as a trophic factor important in axonal or dendritic sprouting. It is also possible that EGF could serve as a neurotransmitter/neuromodulator in the CNS.
Assuntos
Doença de Alzheimer/patologia , Córtex Cerebral/patologia , Receptores ErbB/metabolismo , Hipocampo/patologia , Neurônios/citologia , Idoso , Idoso de 80 Anos ou mais , Doença de Alzheimer/metabolismo , Córtex Cerebral/metabolismo , Receptores ErbB/imunologia , Proteína Glial Fibrilar Ácida/análise , Hipocampo/metabolismo , Humanos , Soros Imunes , Técnicas Imunoenzimáticas , Pessoa de Meia-Idade , Neurônios/metabolismoRESUMO
The physical state of actin in nerve ending preparations and its relationship to the membranes was studied at the ultrastructural level by negative staining with uranyl acetate before and after treatment with muscle heaving meromyosin (HMM). Actin prepared from synaptosomal or synaptic membrane preparations did not polymerize to fiber formation as readily as striated muscle actin under the same conditions. Treatment of these brain actin preparations with HMM, however, resulted in formation of fibers characteristically decorated with arrowheads which were quite similar to those formed with muscle actin. Treatment of the synaptosomal or synaptic membrane fractions themselves with HMM caused the formation of numerous decorated fibers although fibers were not evident before HMM treatment. This did not occur with the presynaptic vesicle fraction. The studies suggest that at least part of the actin is associated with synaptic membranes and is in a partially polymerized or non-polymerized state; polymerization can be induced by HMM.
Assuntos
Actinas , Química Encefálica , Encéfalo/ultraestrutura , Actinas/análise , Animais , Histocitoquímica , Técnicas In Vitro , Microscopia Eletrônica , Músculos/análise , Músculos/ultraestrutura , Subfragmentos de Miosina , Polímeros , Coelhos , Frações Subcelulares/análise , Frações Subcelulares/ultraestrutura , Membranas Sinápticas/análise , Membranas Sinápticas/ultraestrutura , Sinaptossomos/análise , Sinaptossomos/ultraestruturaRESUMO
Mature mouse spinal cord-ganglion cultures, which contain both peripheral and central nervous system as one unit, were infected with herpes simplex virus type 1 (HSV 1) or type 2 (HSV 2) and observed by bright field microscopy for up to 72 hours. There was degeneration of both central and peripheral myelin in cultures infected with either virus, but the pattern of peripheral myelin degeneration associated with HSV 1-infected cultures was differrnt from that in HSV 2-infected cultures. Type 1 was charcterized by focal dilatations; type 2 by "sausage-shaped" swellings, and the cytopathic effect of HSV 2 both began (6 hours p.i.) and was completed (36 hours p.i.) earlier than in cultures infected with HSV 1 (12 hours and 48 hours p.i. respectively). In central nervous tissue, the apperance of degenerating myelin after infection with HSV 1 was indistinguishable from that in HSV 2-infected cultures, but the rate of myelin loss was greater in cultures infected with the type 2 virus. Evidence is presented which suggests that, at least in the peripheral nervous system,myelin degeneration did not appear to be dependent on neuronal or axonal dysfunction or death, but was a direct result of virus infection.
Assuntos
Gânglios Espinais/patologia , Herpes Simples/patologia , Medula Espinal/patologia , Animais , Efeito Citopatogênico Viral , Herpes Simples/microbiologia , Camundongos , Degeneração Neural , Técnicas de Cultura de Órgãos , Especificidade de Órgãos , Simplexvirus/patogenicidade , Especificidade da EspécieRESUMO
Animal studies have shown the importance of the nucleus tractus solitarii, a collection of neurons in the brain stem, in the acute regulation of blood pressure. Impulses arising from the carotid and aortic baroreceptors converge in this center, where the first synapse of the baroreflex is located. Stimulation of the nucleus tractus solitarii provides an inhibitory signal to other brain stem structures, particularly the rostral ventrolateral medulla, resulting in a reduction in sympathetic outflow and a decrease in blood pressure. Conversely, experimental lesions of the nucleus tractus solitarii lead to loss of baroreflex control of blood pressure, sympathetic activation, and severe hypertension in animals. In humans, baroreflex failure due to deafferentation of baroreceptors has been previously reported and is characterized by episodes of severe hypertension and tachycardia. We present a patient with an undetermined process of the central nervous system characterized pathologically by ubiquitous infarctions that were particularly prominent in the nucleus tractus solitarii bilaterally but spared the rostral ventrolateral medulla. Absence of a functioning baroreflex was evidenced by the lack of reflex tachycardia to the hypotensive effects of sodium nitroprusside, exaggerated pressor responses to handgrip and cold pressor test, and exaggerated depressor responses to meals and centrally acting alpha 2-agonists. This clinicopathological correlate suggests that the patient's baroreflex failure can be explained by the unique combination of the destruction of sympathetic inhibitory centers (ie, the nucleus tractus solitarii) and preservation of centers that exert a positive modulation on sympathetic tone (ie, the rostral ventrolateral medulla).
Assuntos
Barorreflexo/fisiologia , Infarto Cerebral/fisiopatologia , Hipertensão/etiologia , Núcleo Solitário/irrigação sanguínea , Adulto , Pressão Sanguínea , Infarto Cerebral/complicações , Ingestão de Alimentos/fisiologia , Humanos , Masculino , Pressorreceptores/fisiopatologia , Fumar/fisiopatologia , Núcleo Solitário/fisiopatologiaRESUMO
The morphological distribution of quinolinic acid phosphoribosyltransferase (QPRT), the degradative enzyme of the endogenous excitotoxin quinolinic acid, was studied in the human hippocampal formation and parahippocampal gyrus by immunohistochemical techniques. In seven neurologically normal human brains obtained at autopsy, QPRT-immunoreactivity (QPRT-i) was found in both glial cells and neurons. Glial cells exhibiting QPRT-immunoreactivity morphological features of astrocytes, were observed in all hippocampal subfields. The polymorphic layer of the dentate gyrus contained the highest density of QPRT-i glial cells. Numerous QPRT-i glial cells were also found along both sides of the fused hippocampal fissure and in the white matter including the alveus of Ammon's horn, whereas only a few were observed in the granule cell layer and the stratum pyramidale. Neurons containing QPRT-i were found mainly in the subiculum and in the strata oriens and pyramidale of CA1. They were mostly small and polymorphic or fusiform, thus indicating that they may belong to a subpopulation of interneurons. Moderate numbers of QPRT-i glial cells and neurons were also observed throughout layers II-VI of parahippocampal cortex. The localization of QPRT-i in selected glial cells and neurons suggests that in the regions examined these cellular elements might play specific roles in the regulation of quinolinic acid function.
Assuntos
Hipocampo/enzimologia , Pentosiltransferases/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Córtex Cerebral/citologia , Córtex Cerebral/enzimologia , Feminino , Hipocampo/citologia , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-IdadeRESUMO
A histochemical study of substantia nigra and locus ceruleus from postmortem brains showed the presence of small spherical cytoplasmic bodies stained selectively by the anionic phosphotungstic acid-hematoxylin (PTAH) stain at a pH of 2.5. The metachromatic reaction to PTAH indicates that these protein bodies contain a protein rich in free basic amino groups. The protein bodies are localized within the neuronal perikaryon as well as in their dendritic processes. These bodies abundantly present in the substantia nigra and locus ceruleus of normal brains were noticeably reduced or absent in parkinsonian brains. Lewy bodies when present show that their core gives the same metachromatic reaction to PTAH as do the protein bodies. These findings suggest that an abnormality of protein synthesis in the substantia nigra and locus ceruleus of parkinsonian brains may be related to the absence of protein bodies and the formation of Lewy bodies and play a role in pathogenesis of the parkinsonian state.
Assuntos
Locus Cerúleo/ultraestrutura , Proteínas do Tecido Nervoso/análise , Doença de Parkinson/patologia , Substância Negra/ultraestrutura , Idoso , Citoplasma/ultraestrutura , Histocitoquímica , Humanos , Locus Cerúleo/metabolismo , Pessoa de Meia-Idade , Neurônios/análise , Neurônios/ultraestrutura , Doença de Parkinson/metabolismo , Substância Negra/metabolismoRESUMO
This study investigates a canine model of experimental brain tumor. Particularly addressed was the usefulness of gadolinium contrast-enhanced MRI for differentiating brain tumor tissue from cerebral edema. Cultured canine glioma cells were injected into the left hemispheres of six adult mongrel dogs. All dogs developed brain tumors. Serum samples drawn prior to and serially after tumor inoculation showed development of antibodies reactive to the tumor. All tumors were visualized with MRI. Contrast-enhanced T1-weighted imaging was the most sensitive with gadolinium producing tumor enhancement due to blood-brain barrier breakdown. Gross and microscopic autopsy findings correlated well with MRIs.
Assuntos
Neoplasias Encefálicas/diagnóstico , Glioma/diagnóstico , Espectroscopia de Ressonância Magnética , Animais , Anticorpos Antineoplásicos/biossíntese , Neoplasias Encefálicas/imunologia , Neoplasias Encefálicas/patologia , Linhagem Celular , Cães , Gadolínio , Gadolínio DTPA , Glioma/imunologia , Glioma/patologia , Compostos Organometálicos , Ácido PentéticoRESUMO
The localization and distribution of quinolinic acid phosphoribosyltransferase, the degradative enzyme of the endogenous excitotoxin quinolinic acid, were studied in the post mortem human neostriatum by immunohistochemistry. In eight neurologically normal human brains, quinolinic acid phosphoribosyltransferase immunoreactivity was detected in both glial cells and neurons. Typically, glial cells containing quinolinic acid phosphoribosyltransferase immunoreactivity had numerous processes radiating from the cell bodies. In Nissl-counterstained sections, most quinolinic acid phosphoribosyltransferase-immunoreactive glial cells showed round, large and pale nuclei. These morphological features indicate that they are probably astrocytes. Neurons containing quinolinic acid phosphoribosyltransferase immunoreactivity had different sizes and shapes and were tentatively classified into three subpopulations. Most were medium-sized cells with ovoid or elongated perikarya. Small quinolinic acid phosphoribosyltransferase-immunoreactive neurons, often spheroid in shape, were particularly noted in a zone of the caudate nucleus adjacent to the lateral ventricle. A few large quinolinic acid phosphoribosyltransferase-positive neurons were also present in both the caudate and putamen. The somatic and dendritic morphology of quinolinic acid phosphoribosyltransferase-immunoreactive neurons closely resembles that of aspiny neurons seen in Golgi preparations. The localization of the specific quinolinic acid-catabolizing enzyme in distinct populations of neostriatal cells suggests specific functional correlates. It remains to be examined how the anatomical organization of quinolinic acid phosphoribosyltransferase immunoreactivity relates to the degradation of quinolinic acid in the striatum, and if the morphological characteristics and distribution of quinolinic acid phosphoribosyltransferase-immunoreactive cells are of relevance for the pathogenesis of neurodegenerative basal ganglia disorders.
Assuntos
Núcleo Caudado/enzimologia , Pentosiltransferases/metabolismo , Putamen/enzimologia , Adulto , Idoso , Feminino , Humanos , Doença de Huntington/enzimologia , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Neuroglia/enzimologia , Neurônios/enzimologiaRESUMO
The immunohistochemical localization of the calcium-binding protein, S100 beta, in human nervous system tumors has been examined by using a monoclonal antibody with specificity for the S100 beta polypeptide. S100 beta-specific immunoreactivity is detected in astrocytoma, glioblastoma, Schwannoma, ependymoma, and craniopharyngioma, whereas no reactivity is seen in oligodendroglioma, meningioma, neuroblastoma, or medulloblastoma. These data suggest that analysis of S100 beta localization with these monoclonal antibodies may be useful for research or diagnostic purposes.
Assuntos
Anticorpos Monoclonais/imunologia , Neoplasias Encefálicas/análise , Proteínas S100/análise , Animais , Astrocitoma/análise , Bovinos , Diferenciação Celular , Ependimoma/análise , Glioma/análise , Histocitoquímica , Humanos , Meduloblastoma/análise , Neuroblastoma/análise , Oligodendroglia/análise , Proteínas S100/imunologiaRESUMO
We examined the cellular distribution of lipocortin-1 (L-1), a major physiologic substrate for the epidermal growth factor receptor/kinase, in 122 central nervous system (CNS) and peripheral nervous system (PNS) neoplasms using the peroxidase-antiperoxidase technique with a polyclonal antibody specific for L-1. Extensive L-1 immunoreactivity was demonstrated in many CNS tumors; in 11 of 21 glioblastoma multiformes, in five of 12 anaplastic astrocytomas, and in five of 14 astrocytomas. Significant numbers of immunoreactive ependymocytes or astrocytes were also seen in six of 13 ependymomas. In contrast, no immunostaining was detected in the oligodendrocytes in any of ten oligodendrogliomas. PNS tumors, found in two of five malignant nerve sheath tumors, 13 of 15 schwannomas, 13 of 17 neurofibromas, and 14 of 15 traumatic neuromas, also contained considerable L-1 immunoreactivity in Schwann cells or mast cells. These findings raise the possibility that L-1 may participate in the proliferation or subsequent differentiation of neoplastic astrocytes, ependymocytes, and Schwann cells.
Assuntos
Neoplasias Encefálicas/análise , Glioma/análise , Glicoproteínas/análise , Neoplasias do Sistema Nervoso Periférico/análise , Fosfolipases/antagonistas & inibidores , Neoplasias da Medula Espinal/análise , Anexinas , Neoplasias Encefálicas/imunologia , Neoplasias Encefálicas/patologia , Humanos , Imuno-Histoquímica , Neurilemoma/análise , Neurilemoma/patologia , Neurofibroma/análise , Neurofibroma/patologia , Neoplasias do Sistema Nervoso Periférico/patologia , Neoplasias da Medula Espinal/imunologia , Neoplasias da Medula Espinal/patologiaRESUMO
The authors have examined the cellular distribution of lipocortin-1 (L-1) in the normal and diseased central nervous system (CNS) using the peroxidase-antiperoxidase (PAP) technique with a polyclonal antibody specific for L-1. L-1 immunoreactivity was evaluated in the frontal cortex, parahippocampal gyrus/lateral ventricle, cerebellum, medulla, and spinal cord from 27 normal human fetuses, neonates, and adults without neurologic disease and in these same regions and representative lesions from 35 patients with diseases producing varying degrees of astrocytosis, including intraparenchymal hemorrhage; embolic, thrombotic, or traumatic infarctions; and Alzheimer's disease (AD). L-1 immunoreactivity was identified in ependymocytes, choroid plexus epithelia, and scattered subependymal astrocytes throughout the ventricular system from 15 weeks gestation through 82 years of age in both normal and diseased CNSs. L-1 immunoreactivity was also detected in reactive astrocytes and many macrophages surrounding each infarction regardless of site or pathogenesis and in scattered reactive astrocytes in people with AD or SDAT. The limited distribution of L-1 in CNS is consistent with the low amounts of L-1 found in brain and suggests that L-1 may participate in the normal function of ependymocytes and the pathophysiology of reactive astrocytosis.
Assuntos
Astrócitos/metabolismo , Proteínas de Ligação ao Cálcio/metabolismo , Doenças do Sistema Nervoso Central/metabolismo , Sistema Nervoso Central/metabolismo , Gliose/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Anexinas , Criança , Pré-Escolar , Epêndima/citologia , Epêndima/metabolismo , Feminino , Morte Fetal/metabolismo , Humanos , Técnicas Imunoenzimáticas , Lactente , Recém-Nascido , Doenças do Prematuro/metabolismo , Masculino , Pessoa de Meia-Idade , Fosfolipases/antagonistas & inibidores , Fosfolipases/metabolismo , GravidezRESUMO
Quinolinic acid (QUIN) is an endogenous excitatory amino acid, which is elevated in brain tissues or cerebrospinal fluid (CSF) in several acute and chronic inflammatory central nervous system (CNS) diseases. The functional significance of this elevation is unknown but speculations of excitotoxicity have been raised. We have begun to address the pathologic consequences of elevated CSF QUIN by studying the effects of intracerebroventricular (i.cv) administration of QUIN on regional choline acetyltransferase (ChAT) activity, somatostatin content and glucose metabolism in the rat brain. QUIN (12 and 60 nmol) i.cv administration once a day for 7 days (total dose; 84 and 420 nmol, respectively) had minimal effect on somatostatin content and no effect on ChAT activity. In contrast, following continuous i.cv infusion of QUIN for 14 days using an osmotic minipump (480 nmol), ChAT activity dropped in the hippocampus and the striatum and somatostatin content was reduced in the frontal cortex, hippocampus, striatum and amygdala. Moreover, following the QUIN infusion, glucose utilization decreased in the basal nucleus of Meynert, frontal cortex, and portions of the basal ganglia and the limbic system. These results indicate that subchronic i.cv infusion of QUIN to rats results in selective regional neurochemical and metabolic changes distributed throughout the CNS. These results suggest target brain areas and transmitter systems which may be associated with neurologic syndromes characterized by elevated CSF QUIN levels.
Assuntos
Comportamento Animal/efeitos dos fármacos , Encéfalo/efeitos dos fármacos , Ácido Quinolínico/farmacologia , Animais , Encéfalo/metabolismo , Encéfalo/patologia , Colina O-Acetiltransferase/metabolismo , Glucose/metabolismo , Injeções Intraventriculares , Masculino , Ácido Quinolínico/líquido cefalorraquidiano , Ratos , Ratos Sprague-Dawley , Somatostatina/metabolismoRESUMO
We demonstrate here that electrical stimulation of organotypic cultures of rat hippocampus results in the prompt release of significant amounts of Zn(II) by a fluorescence microscopic method. The fluorescence imaging of free Zn(II) is achieved using a highly selective biosensing indicator system consisting of human apo-carbonic anhydrase II (apoCAII) and a fluorescent aryl sulfonamide inhibitor of the enzyme, ABD-N. The apoenzyme and ABD-N in the absence of Zn(II) exhibit weak, reddish fluorescence typical of the ABD-N alone; when Zn(II) is added it binds to the apoenzyme (K(D) = 4 pM), which strongly promotes binding of ABD-N to the holoenzyme (K(D) = 0.9 microM). Binding of ABD-N to the holoenzyme results in a 9-fold increase in apparent quantum yield, significant blue shifts in excitation and emission, an increase in average fluorescence lifetime, a 4-fold increase in the ratio of intensities at 560 and 680 nm, and a large increase in anisotropy. Prior to stimulation, cultures immersed in phosphate-buffered saline with glucose and apoCAII with ABD-N emitted negligible fluorescence, but within 20 s after electrical stimulation a diffuse cloud of greenish fluorescence emerged and subsequently covered most of the culture, indicating release of zinc into the extracellular medium.