Physicochemical and microbiological characteristics of urban aerosols in Krakow (Poland) and their potential health impact.

Eight aerosol samples were collected in Krakow using a low-volume sampler in February and March 2019 during variable meteorological conditions and times of the day, to study their single particles' properties (size, morphology and chemical composition analyzed using a scanning electron microscope fitted with an energy-dispersive spectrometer) and microbiological characteristics. The content of particles of different chemical compositions larger than 2.5 µm was low. Considering the number of the particles, submicron particles strongly dominated with a high content of ultrafine particles (nanoparticles). Tar ball-type particles were relatively common in the studied samples, while soot was the dominant component. Soot was present as small agglomerates composed of few particles, but also as bigger agglomerates. Metal-containing particles of various chemical characteristics were abundant, with transition metals commonly occurring in these particles. The physicochemical characteristics of aerosols indicate that despite a relatively low mass concentration, their adverse health impact could be very strong because of the high content of nanoparticles, the abundance of soot and other fuel combustion-related particles, and the high incidence of transition metal-rich particles. Microbiological analysis was based on cultures on both solid and liquid agar. The MALDI-TOF method was used for species identification-for bacteria and fungi. Twelve different species of bacteria were isolated from the collected samples of aerosols. The most frequently isolated species was Gram-positive sporulating Bacillus licheniformis. The isolated mold fungi were of the genus Aspergillus.

Airborne Microplastic in the Atmospheric Deposition and How to Identify and Quantify the Threat: Semi-Quantitative Approach Based on Kraków Case Study.

Airborne microplastic is an emerging and widespread pollutant yet is still under-characterised and insufficiently understood. Detailed description of microplastic air pollution is crucial as it has been identified in human lungs and remote locations, highlighting the atmosphere as a medium of MP dispersion and transportation. The lack of standardization of methods for measuring and further monitoring of microplastic pollution is an obstacle towards assessment of health risks. Since the first recognition of MP presence in the atmosphere of Krakow in 2019, this research was conducted to further characterise and develop the methods for qualitative and quantitative analysis of airborne microplastic (attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR); pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS); scanning electron microscopy-energy dispersive spectroscopy SEM-EDS) and pre-treatment of samples. The data were gathered in seven cycles from June 2019 to February 2020. The methods used in the study allowed the identification and analysis of the changing ratio of the different types of synthetic polymers identified in the atmospheric fallout (low-density polyethylene, nylon-66, polyethylene, polyethylene terephthalate, polypropylene and polyurethane). Observations of interactions between microplastic particles and the environment were conducted with analyses of surface changes due to degradation. Different phases attached to the microplastics surfaces, with some of the inorganic contaminants transported on these surfaces determined also to be of anthropogenic origin. The methodology proposed in this study allows further characterisation of microplastic from multiple locations to provide highly comparable data, leading to identification of the sources of this phenomenon, as well as seasonal changes.