RESUMO
Severe Coronavirus Disease 2019 (COVID-19) is characterized by numerous complications, complex disease, and high mortality, making its treatment a top priority in the treatment of COVID-19. Integrated traditional Chinese medicine (TCM) and western medicine played an important role in the prevention, treatment, and rehabilitation of COVID-19 during the epidemic. However, currently there are no evidence-based guidelines for the integrated treatment of severe COVID-19 with TCM and western medicine. Therefore, it is important to develop an evidence-based guideline on the treatment of severe COVID-19 with integrated TCM and western medicine, in order to provide clinical guidance and decision basis for healthcare professionals, public health personnel, and scientific researchers involved in the diagnosis, treatment, and care of COVID-19 patients. We developed and completed the guideline by referring to the standardization process of the "WHO handbook for guideline development", the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system, and the Reporting Items for Practice Guidelines in Healthcare (RIGHT).
Assuntos
Antivirais/uso terapêutico , Tratamento Farmacológico da COVID-19 , Medicamentos de Ervas Chinesas/uso terapêutico , Infectologia/tendências , Medicina Tradicional Chinesa/tendências , SARS-CoV-2/efeitos dos fármacos , Antivirais/efeitos adversos , COVID-19/diagnóstico , COVID-19/virologia , Consenso , Técnica Delphi , Medicamentos de Ervas Chinesas/efeitos adversos , Medicina Baseada em Evidências/tendências , Interações Hospedeiro-Patógeno , Humanos , Gravidade do Paciente , SARS-CoV-2/patogenicidade , Resultado do TratamentoRESUMO
Two new phenolic glycosides (1 and 2) and two new isocoumarin glycosides (3 and 4), along with 14 known compounds (5-18), were isolated from the stems of Homalium paniculiflorum. Their structures were established on the basis of extensive spectroscopic analyses and chemical methods. All new compounds were evaluated for their anti-inflammatory activities via examining the inhibitory activity on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in mouse macrophage RAW 264.7 cells in vitro. Compounds 1 and 4 exhibited inhibitory activities with IC50 values of 30.23 ± 1.23 µM and 19.36 ± 0.19 µM, respectively.
Assuntos
Glicosídeos/química , Inflamação/tratamento farmacológico , Isocumarinas/química , Salicaceae/química , Animais , Humanos , Inflamação/induzido quimicamente , Lipopolissacarídeos/toxicidade , Camundongos , Óxido Nítrico/antagonistas & inibidores , Óxido Nítrico/biossíntese , Fenol/química , Caules de Planta/química , Células RAW 264.7/efeitos dos fármacosRESUMO
Two new sulphur-containing amides, glylucidamides C-D (1-2), along with twelve known analogues (3-14) were isolated and characterised from the leaves of Glycosmis lucida. The chemical structures of 1 and 2 were elucidated by extensive spectroscopic methods and the known compounds were identified by comparisons their data with those reported in the literatures. All new compounds were evaluated for their anti-inflammatory activities via examining the inhibitory activity on nitric oxide (NO) production induced by lipopolysaccharide in mouse macrophage RAW 264.7 cells in vitro.
RESUMO
Fourteen new sulphur-containing amides, glycocramides A-N (1-14), as well as nine known analogues (15-23) were isolated and characterized from Glycosmis craibii Tanaka. The chemical structures of new sulphur-containing amides 1-14 were ambiguously elucidated by extensive spectroscopic methods, while the known compounds 15-23 were identified by the comparison of their experimental spectral data with those described data in the literatures. The antiproliferative effects of all isolated sulphur-containing amides were evaluated in vitro. As a result, part of sulphur-containing amides showed remarkable inhibitory effects against MGC-803 cell line with IC50 values ranging from 13.12 ± 0.10 to 20.03 ± 0.13 µM. These research results suggest that the sulphur-containing amides are potentially to be developed as a new natural anti-tumor drugs.
Assuntos
Amidas , Rutaceae , Amidas/farmacologia , Amidas/química , Estrutura Molecular , Enxofre , Extratos Vegetais/química , Rutaceae/química , Linhagem Celular TumoralRESUMO
A phytochemical investigation on the stems of Glycosmis puberula var. craibii led to the isolation of a new indole alkaloid (named glycosmiscrol A, 1), together with four known compounds (2-5). The new structure was elucidated by detailed analysis of comprehensive spectroscopic methods. All isolated compounds were evaluated for their antiproliferative activities against five human cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7 and SW480 in vitro. Compounds 1-5 showed significant antiproliferative effects with IC50 values ranging from 0.16 to 8.58 µM.
Assuntos
Antineoplásicos Fitogênicos , Rutaceae , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Humanos , Alcaloides Indólicos/farmacologia , Estrutura Molecular , Caules de PlantaRESUMO
The phytochemical investigation of the stems of Homalium stenophyllum afforded seven new phenolic glycosides (1-5 and 8-9) and two known compounds (6 and 7). Their structures were elucidated by comprehensive analyses of NMR spectroscopic, mass spectrometric data and chemical hydrolysis. Additionally, their anti-inflammatory activities against the NO production in LPS-induced macrophages were evaluated.
Assuntos
Glicosídeos , Fenóis , Salicaceae/química , Anti-Inflamatórios/farmacologia , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Macrófagos/efeitos dos fármacos , Estrutura Molecular , Fenóis/isolamento & purificação , Fenóis/farmacologia , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Caules de Planta/químicaRESUMO
A preliminary phytochemical investigation of the stems of the endangered plant Ulmus elongata led to the isolation of a new coumarin derivative (named ulmuselactone A, 1) and eight known compounds (2-9). The new structure was elucidated by detailed analysis of comprehensive spectroscopic methods, and its absolute configuration was established by comparing experimental and calculated electronic circular dichroism (ECD) spectra. The isolated compounds were evaluated for their antibacterial activities.
Assuntos
Ulmus , Antibacterianos/farmacologia , Cumarínicos/farmacologia , Compostos Fitoquímicos , Extratos VegetaisRESUMO
One new norisoprenoid 3,9-dihydroxy dihydro actinidiolide (1), together with eleven known compounds (2-12), were isolated from ethanol extract of the leaves of Ficus pumila collected in Hainan Province, China. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. Compounds 1, 2, 4, 5, 8 and 10-12 were isolated from the F. pumila for the first time. All compounds were evaluated for their cytotoxic activity. Compounds 3 and 9 showed weak cytotoxic activity against Hela, MCF-7 and A549 cell lines.
Assuntos
Ficus/química , Norisoprenoides/isolamento & purificação , Linhagem Celular Tumoral , Humanos , Norisoprenoides/química , Extratos Vegetais/análise , Folhas de Planta/químicaRESUMO
A new morphinandienone alkaloid, fissistigmine A (1), together with three known alkaloids (2-4), were isolated and identified from the stems of Fissistigma tungfangense. Among them, fissistigmine A (1) represents the first example of a novel naturally occurring morphinandienone alkaloid with a unique cleavage of the C-9-N-17 bond. All isolated compounds were evaluated for their anti-rheumatoid arthritis activities via examining their anti-proliferative effects on synoviocytes in vitro. Compound 1 exhibited inhibitory effect on the proliferation of synoviocytes with an IC50 value of 114.6 ± 2.2 µM.
Assuntos
Alcaloides/isolamento & purificação , Annonaceae/química , Morfinanos/isolamento & purificação , Alcaloides/química , Antirreumáticos/isolamento & purificação , Proliferação de Células/efeitos dos fármacos , Morfinanos/química , Caules de Planta/química , Sinoviócitos/efeitos dos fármacosRESUMO
Phytochemical investigation of the stems of Vatica mangachapoi (Dipterocarpaceae) led to the isolation and structural elucidation of twenty-seven oligostilbenes (1-27), including a new natural compound 1. The structure of 1 was elucidated on the basis of spectroscopic analyses including NMR, MS and ECD data, and the known compounds were identified by comparisons with those reported in the literature. The absolute configuration of 1 was first time determined by a combination of NOESY spectrum and quantum chemical computation. Among of isolates were tested for their anti-osteoporosis and anti-HIV-1 activities in vitro by the MTT method. Moreover, the chemotaxonomic significance of these compounds was summarised.
Assuntos
Dipterocarpaceae/química , Estilbenos/isolamento & purificação , Fármacos Anti-HIV/farmacologia , Espectroscopia de Ressonância Magnética , Osteoporose/tratamento farmacológico , Caules de Planta/química , Estilbenos/química , Estilbenos/classificação , Estilbenos/farmacologiaRESUMO
One new lignan derivative 2,3-dimethyl-4-(4'-hydroxy-3',5'-dimethoxyphenyl)-6-hydroxy-7-methoxy-naphthalene (1), together with four known analogues (2-5), were isolated from acetone extract of the fruiting bodies of Ganoderma lipsiense collected in the Hainan Province, China. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. Compound 1 showed insecticidal activity against newly hatched karvae of Helicoverpa armigera Hubner. Compounds 1-5 showed moderate antibacterial activity against Staphylococcus aureus with the same MIC value of 1.25 µg/mL.
Assuntos
Antibacterianos/farmacologia , Ganoderma/química , Inseticidas/farmacologia , Lignanas/farmacologia , Animais , Antibacterianos/química , China , Avaliação Pré-Clínica de Medicamentos , Carpóforos/química , Inseticidas/química , Lignanas/química , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Estrutura Molecular , Mariposas/efeitos dos fármacos , Espectrometria de Massas por Ionização por Electrospray , Staphylococcus aureus/efeitos dos fármacosRESUMO
A new dihydrochalcone glycoside, phloretin-4-O-ß-D-glucopyranoside (1), together with seven known flavonoids (2-8), were isolated from the stems of Homalium stenophyllum. The structure of 1 was elucidated by extensive spectroscopic methods and the known compounds were identified by comparisons with data reported in the literature. The known compounds (2-8) were isolated from the genus Homalium for the first time. All compounds were evaluated for their antibacterial activities against six pathogenic bacteria in vitro.
Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Chalconas/química , Glicosídeos/química , Salicaceae/química , Antibacterianos/isolamento & purificação , Flavonoides/química , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Estrutura Molecular , Caules de Planta/químicaRESUMO
One new lignan derivative 2,3-dimethyl-4-(4-methoxyphenyl)-6,7-dihydroxynaphthalene (1), together with five known compounds (2-6), were isolated from ethanol extract of the branches and leaves of Combretum alfredii Hance collected in Hainan Province, China. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. Compounds 1, 2, 5 and 6 were isolated from the genus of Combretum for the first time. All compounds were evaluated for their antibacterial activities. Compounds 1 and 2 showed significant antibacterial activities against six pathogenic bacteria.
Assuntos
Antibacterianos/isolamento & purificação , Combretum/química , Lignanas/isolamento & purificação , Lignanas/química , Lignanas/farmacologia , Naftóis , Extratos Vegetais/análise , Folhas de Planta/químicaRESUMO
One new alkaloid consanguine B (1), together with 10 known alkaloids (2-11), were isolated from ethanol extract of the branches and leaves of Polyalthia obliqua Hook.f. & Thomson collected in the Hainan Province, China. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. All compounds were evaluated for their cytotoxic activities. Compound 1 showed weak cytotoxic activities against Hela and MCF-7 human cancer cell lines.