RESUMO
An anaerobic, Gram-positive, rod-shaped, motile and spore-forming bacterium, designated strain ZCY20-5T, was isolated from pit clay of Chinese strong-aroma type Baijiu (Chinese liquor). Phylogenetic analyses based on 16S rRNA gene and genome sequences showed that strain ZCY20-5T belonged to the genus Caproicibacterium, family Oscillospiracheae, but it showed low similarity to the type species Caproicibacterium amylolyticum LBM18003T (98.00â%) and Caproicibacterium lactatifermentans LBM19010T (95.67â%). In anaerobic yeast extract medium, growth was observed at 20-45â°C (optimum, 35-40â°C), at pH 4.0-9.0 (optimum, pH 6.5-7.0) and with 0.0-2.0â% NaCl (w/v). The predominant fatty acids were C16â:â0, C14â:â0, C13 3-OH and C16 3-OH, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, three phospholipids of unknown structure containing glucosamine and two unidentified phospholipids. Strain ZCY20-5T exhibited an 81.32â% pairwise average nucleotide identity value, a 78.98â% average amino acid identity value and a 22.30â% digital DNA-DNA hybridization value compared to its closest relative C. amylolyticum LBM18003T. Based on morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic results, strain ZCY20-5T represents a novel species of Caproicibacterium, and the type strain is ZCY20-5T (=MCCC 1A19399T=KCTC 25590T).