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1.
Sensors (Basel) ; 23(19)2023 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-37836969

RESUMO

In wireless communication, to fully utilize the spectrum and energy efficiency of the system, it is necessary to obtain the channel state information (CSI) of the link. However, in Frequency Division Duplexing (FDD) systems, CSI feedback wastes part of the spectrum resources. In order to save spectrum resources, the CSI needs to be compressed. However, many current deep-learning algorithms have complex structures and a large number of model parameters. When the computational and storage resources are limited, the large number of model parameters will decrease the accuracy of CSI feedback, which cannot meet the application requirements. In this paper, we propose a neural network-based CSI feedback model, Mix_Multi_TransNet, which considers both the spatial characteristics and temporal sequence of the channel, aiming to provide higher feedback accuracy while reducing the number of model parameters. Through experiments, it is found that Mix_Multi_TransNet achieves higher accuracy than the traditional CSI feedback network in both indoor and outdoor scenes. In the indoor scene, the NMSE gains of Mix_Multi_TransNet are 4.06 dB, 4.92 dB, 4.82 dB, and 6.47 dB for compression ratio η = 1/8, 1/16, 1/32, 1/64, respectively. In the outdoor scene, the NMSE gains of Mix_Multi_TransNet are 3.63 dB, 6.24 dB, 4.71 dB, 4.60 dB, and 2.93 dB for compression ratio η = 1/4, 1/8, 1/16, 1/32, 1/64, respectively.

2.
Inflamm Res ; 64(10): 799-808, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26298161

RESUMO

OBJECTIVE: The purpose of the present study was to evaluate the potential therapeutic effects of NZ on lipopolysaccharide (LPS)-induced RAW264.7 cells and explore its underlying mechanisms. METHODS: The effect of NZ on NO generation in LPS-activated macrophage was measured by Griess assay. The concentrations of TNF-α, IL-18, IL-1ß were analyzed with ELISA kits. The LPS-induced production of reactive oxygen species (ROS) was determined by flow cytometry. The protein expressions of TLR4, NF-κB and NLRP3 signaling pathway were investigated with Western blot analysis. RESULTS: It was shown that NZ significantly reduced the production of NO and the generation of pro-inflammatory cytokines in LPS-induced RAW264.7 cells. In addition, NZ markedly inhibited the up-regulation of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and the activation of nuclear factor kappa B (NF-κB) in LPS-stimulated RAW 264.7 macrophages. Of note, NZ suppressed the expression of the inflammasome component such as NOD-like receptor 3(NLRP3), apoptosis-associated speck-like protein containing CARD(ASC), as well as the levels of cytokines including Interleukin-18(IL-18) and Interleukin-1ß(IL-1ß). CONCLUSION: These results indicated that NZ inhibited the generations of NO and pro-inflammatory cytokines by suppressing TLR4/MyD88/NF-κB pathway, suggesting that NZ could be an effective candidate for ameliorating LPS-induced inflammatory responses.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Proteínas de Transporte/efeitos dos fármacos , Inflamassomos/efeitos dos fármacos , NF-kappa B/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos , Receptor 4 Toll-Like/antagonistas & inibidores , Animais , Caspase 1/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Citocinas/metabolismo , Lipopolissacarídeos/farmacologia , Camundongos , Fator 88 de Diferenciação Mieloide/biossíntese , Proteína 3 que Contém Domínio de Pirina da Família NLR , Óxido Nítrico/metabolismo , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/farmacologia , Células RAW 264.7 , Espécies Reativas de Oxigênio/metabolismo
3.
Front Plant Sci ; 15: 1415884, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39119504

RESUMO

The pollination process of kiwifruit flowers plays a crucial role in kiwifruit yield. Achieving accurate and rapid identification of the four stages of kiwifruit flowers is essential for enhancing pollination efficiency. In this study, to improve the efficiency of kiwifruit pollination, we propose a novel full-stage kiwifruit flower pollination detection algorithm named KIWI-YOLO, based on the fusion of frequency-domain features. Our algorithm leverages frequency-domain and spatial-domain information to improve recognition of contour-detailed features and integrates decision-making with contextual information. Additionally, we incorporate the Bi-Level Routing Attention (BRA) mechanism with C3 to enhance the algorithm's focus on critical areas, resulting in accurate, lightweight, and fast detection. The algorithm achieves a m A P 0.5 of 91.6% with only 1.8M parameters, the AP of the Female class and the Male class reaches 95% and 93.5%, which is an improvement of 3.8%, 1.2%, and 6.2% compared with the original algorithm. Furthermore, the Recall and F1-score of the algorithm are enhanced by 5.5% and 3.1%, respectively. Moreover, our model demonstrates significant advantages in detection speed, taking only 0.016s to process an image. The experimental results show that the algorithmic model proposed in this study can better assist the pollination of kiwifruit in the process of precision agriculture production and help the development of the kiwifruit industry.

4.
Foods ; 13(17)2024 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-39272541

RESUMO

Post-harvest selection of high-quality Sichuan pepper is a critical step in the production process. To achieve this, a visual system needs to analyze Sichuan pepper with varying postures and maturity levels. To quickly and accurately sort high-quality fresh Sichuan pepper, this study proposes a multi-scale frequency domain feature fusion module (MSF3M) and a multi-scale dual-domain feature fusion module (MS-DFFM) to construct a multi-scale, multi-domain fusion algorithm for feature fusion of Sichuan pepper images. The MultiDomain YOLOv8 Model network is then built to segment and classify the target Sichuan pepper, distinguishing the maturity level of individual Sichuan peppercorns. A selection method based on the average local pixel value difference is proposed for sorting high-quality fresh Sichuan pepper. Experimental results show that the MultiDomain YOLOv8-seg achieves an mAP50 of 88.8% for the segmentation of fresh Sichuan pepper, with a model size of only 5.84 MB. The MultiDomain YOLOv8-cls excels in Sichuan pepper maturity classification, with an accuracy of 98.34%. Compared to the YOLOv8 baseline model, the MultiDomain YOLOv8 model offers higher accuracy and a more lightweight structure, making it highly effective in reducing misjudgments and enhancing post-harvest processing efficiency in agricultural applications, ultimately increasing producer profits.

5.
Front Cell Neurosci ; 11: 154, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28603491

RESUMO

NOSH-NBP, a novel nitric oxide (NO) and hydrogen sulfide (H2S)-releasing hybrid, protects brain from ischemic stroke. This study mainly aimed to investigate the therapeutic effect of NOSH-NBP on ischemic stroke and the underlying mechanisms. In vivo, transient middle cerebral artery occlusion (tMCAO) was performed in C57BL/6 mice, with NO-NBP and H2S-NBP as controls. NO and H2S scavengers, carboxy-PTIO and BSS, respectively, were used to quench NO and H2S of NOSH-NBP. In vitro, BV2 microglia/BMDM were induced to the M1/2 phenotype, and conditioned medium (CM) experiments in BV2 microglia, neurons and b.End3 cerebral microvascular endothelial cells (ECs) were performed. Microglial/macrophage activation/polarization was assessed by flow cytometry, Western blot, RT-qPCR, and ELISA. Neuronal and EC survival was measured by TUNEL, flow cytometry, MTT and LDH assays. Transmission electron microscopy, EB extravasation, brain water content, TEER measurement and Western blot were used to detect blood-brain barrier (BBB) integrity and function. Interestingly, NOSH-NBP significantly reduced cerebral infarct volume and ameliorated neurological deficit, with superior effects compared with NO-NBP and/or H2S-NBP in mice after tMCAO. Both NO and H2S-releasing groups contributed to protection by NOSH-NBP. Additionally, NOSH-NBP decreased neuronal death and attenuated BBB dysfunction in tMCAO-treated mice. Furthermore, NOSH-NBP promoted microglia/macrophage switch from an inflammatory M1 phenotype to the protective M2 phenotype in vivo and in vitro. Moreover, the TLR4/MyD88/NF-κB pathway and NLRP3 inflammasome were involved in the inhibitory effects of NOSH-NBP on M1 polarization, while peroxisome proliferator activated receptor gamma signaling contributed to NOSH-NBP induced M2 polarization. These findings indicated that NOSH-NBP is a potential therapeutic agent that preferentially promotes microglial/macrophage M1-M2 switch in ischemic stroke.

6.
Int Immunopharmacol ; 25(1): 55-64, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25614226

RESUMO

CDDO-Me, initiated in a phase II clinical trial, is a potential useful therapeutic agent for cancer and inflammatory dysfunctions, whereas the therapeutic efficacy of CDDO-Me on LPS-induced acute lung injury (ALI) has not been reported as yet. The purpose of the present study was to explore the protective effect of CDDO-Me on LPS-induced ALI in mice and to investigate its possible mechanism. BalB/c mice received CDDO-Me (0.5mg/kg, 2mg/kg) or dexamethasone (5mg/kg) intraperitoneally 1h before LPS stimulation and were sacrificed 6h later. W/D ratio, lung MPO activity, number of total cells and neutrophils, pulmonary histopathology, IL-6, IL-1ß, and TNF-α in the BALF were assessed. Furthermore, we estimated iNOS, IL-6, IL-1ß, and TNF-α mRNA expression and NO production as well as the activation of the three main MAPKs, AkT, IκB-α and p65. Pretreatment with CDDO-Me significantly ameliorated W/D ratio, lung MPO activity, inflammatory cell infiltration, and inflammatory cytokine production in BALF from the in vivo study. Additionally, CDDO-Me had beneficial effects on the intervention for pathogenesis process at molecular, protein and transcriptional levels in vitro. These analytical results provided evidence that CDDO-Me could be a potential therapeutic candidate for treating LPS-induced ALI.


Assuntos
Lesão Pulmonar Aguda/tratamento farmacológico , Pulmão/efeitos dos fármacos , Neutrófilos/efeitos dos fármacos , Ácido Oleanólico/análogos & derivados , Lesão Pulmonar Aguda/induzido quimicamente , Lesão Pulmonar Aguda/imunologia , Animais , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Citocinas/metabolismo , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Mediadores da Inflamação/metabolismo , Lipopolissacarídeos/imunologia , Pulmão/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Neutrófilos/imunologia , Óxido Nítrico Sintase Tipo II/metabolismo , Ácido Oleanólico/administração & dosagem , Peroxidase/metabolismo
7.
Int Immunopharmacol ; 26(2): 338-48, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25887267

RESUMO

Platycodin D is a major pharmacological constituent of Platycodi Radix with immunomodulatory activity. The present study was designed to investigate how platycodin D (PLD) reveals liver injury in diabetic mice and its mechanism. Fifty mice were divided into five groups randomly: control group, model group, rosiglitazone (ROG, 10 mg/kg) group, PLD (50 mg/kg) group, and PLD (100 mg/kg) group. Diabetes was induced with the injection of alloxan monohydrate (150 mg/kg) subcutaneously, and animals with blood glucose level of ≥250 mg/dl were considered as diabetic mice. After the first day of diabetes induction, the treatments were performed for 8 weeks. Then the animals were anaesthetized, and blood and liver samples were also collected for further assay. PLD significantly decreased the serum levels of glucose, insulin, interleukin-6 (IL-6), interleukin-1ß, tumor necrosis factor-α (TNF-α), and interleukin (IL)-17A and increased IL-10 level in serum. PLD effectively downregulated aspartate transaminase (AST), alanine aminotransferase (ALT), total cholesterol (TC), and triglycerides (TG) in liver. PLD also attenuated liver histological change. In addition, PLD significantly attenuated IL-17A and IL-10 levels in vitro, flow cytometry (FCM) studies also showed that PLD remarkably inhibited Th17 cells and significantly increased Treg cells in liver tissues and spleen cells. Western blot demonstrated PLD inhibited the phosphorylation of JAK and STAT-3 and the expression of RORγt and increased the expression of Foxp3. The findings showed that PLD exerts beneficial effects on alloxan-induced liver injury in mice.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Codonopsis/imunologia , Diabetes Mellitus Experimental/tratamento farmacológico , Fígado/efeitos dos fármacos , Saponinas/administração & dosagem , Linfócitos T Reguladores/efeitos dos fármacos , Células Th17/efeitos dos fármacos , Triterpenos/administração & dosagem , Alanina Transaminase/genética , Alanina Transaminase/metabolismo , Aloxano/administração & dosagem , Animais , Aspartato Aminotransferases/genética , Aspartato Aminotransferases/metabolismo , Glicemia/efeitos dos fármacos , Doença Hepática Induzida por Substâncias e Drogas/imunologia , Colesterol/metabolismo , Citocinas/metabolismo , Diabetes Mellitus Experimental/imunologia , Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Mediadores da Inflamação/metabolismo , Insulina/sangue , Fígado/metabolismo , Fígado/patologia , Camundongos , Camundongos Endogâmicos , Fator de Transcrição STAT3/genética , Fator de Transcrição STAT3/metabolismo , Saponinas/efeitos adversos , Linfócitos T Reguladores/imunologia , Células Th17/imunologia , Triterpenos/efeitos adversos
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