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ObjectiveHigh performance liquid chromatography (HPLC) was used to establish the specific chromatograms of Aurantii Fructus from different origins, and the quality variability of Aurantii Fructus from Sichuan was analyzed and evaluated by combining entropy weighting method and grey correlation method. MethodHPLC was performed on an Agilent Eclipse Plus C18 column (4.6 mm×250 mm, 5 μm) with a gradient elution of methanol (A)-0.1% phosphoric acid aqueous solution as the mobile phase (0-12 min, 25%-33%A; 12-21 min, 33%-41%A; 21-30 min, 41%-42%A; 30-40 min, 42%-59%A; 40-53 min, 59%-72%A; 53-60 min, 72%A; 60-65 min, 72%-100%A; 65-70 min, 100%A; 70~71 min, 100%-25%A; 71-80 min, 25% A) at a flow rate of 1.0 mL·min-1, the injection volume was 10 μL and the detection wavelength was 330 nm. Fifty batches of Aurantii Fructus samples from different origins (Sichuan, Chongqing, Jiangxi and Hunan) were tested, and the similarity evaluation software is used to generate characteristic profiles and compare them with control profile for peak identification, and then to evaluate the similarity of the samples. IBM SPSS 19.0 and SIMCA 14.1 were used to perform multivariate statistical analysis on the results of the samples, and then the entropy weighting method and grey correlation were used to calculate the overall quality score of samples from Sichuan. ResultHPLC specific chromatogram of Aurantii Fructus was established, and 14 common peaks were identified as eriocitrin, neoeriocitrin, narirutin, naringin, hesperidin, neohesperidin, meranzin hydrate, poncirin, meranzin, marmin, nobiletin, 3,3′,4′,5,6,7,8-heptamethoxyflavone, tangeretin and auraptene. And the similarities between the samples from Sichuan and the control chromatogram were all above 0.980. The samples could be classified into four categories according to their main origins by chemical pattern recognition, and the results of cluster analysis, principal component analysis and orthogonal partial least squares-discriminant analysis were all able to discriminate the samples of different main origins effectively. The comprehensive evaluation results of entropy weighting method combined with grey correlation showed that the quality of Aurantii Fructus from Sichuan varied greatly among different origins, and the quality of Aurantii Fructus from Sichuan was ranked as Bazhong>Luzhou>Chongqing>Neijiang. ConclusionIn this study, the characteristic mapping of Aurantii Fructus from Sichuan is established, and combined with the analytical methods of chemometrics and grey correlation, the quality of samples from different origins can be effectively differentiated, which can provide a reference for the comprehensive evaluation and control of the quality of Aurantii Fructus from Sichuan.
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Chinese medicinal material from Fritillaria, Beimu in Chinese, is a commonly used antitussive and expectorant traditional Chinese herbal medicine, with the significant functions of clearing heat and moistening lung,resolving phlegm and relieving cough. Five kinds of Fritillaria were recorded in the 2020 edition of Chinese Pharmacopoeia, including Fritillariae Cirrhosae Bulbus, Fritillariae Ussuriensis Bulbus, Fritillariae Thunbergii Bulbus, Fritillariae Hupehensis Bulbus and Fritillariae Pallidiflorae Bulbus. At present, the reports on Fritillaria mainly focus on the pharmacological effect, chemical composition, identification of authenticity and other aspects, while there were few reports on harvesting and primary processing of original medicinal materials. Fritillaria medicines were perennial medicinal plant with various and complex varieties, their quality and curative effect were greatly affected by harvesting and processing in producing area. The processing method differed according to its variety. Fritillariae Cirrhosae Bulbus mainly from western Sichuan plateau, Fritillariae Pallidiflorae Bulbus from Xinjiang and Fritillariae Ussuriensis Bulbus from Northeast China were mostly harvested from June to July and sun dried directly or dried. But Fritillariae Thunbergii Bulbus and Fritillariae Hupehensis Bulbus from Yangtze River basin were harvested when the plants wilted at the beginning of summer, and auxiliary materials such as shell powder and lime must be added during the processing. At present, the drying methods of Fritillaria were still traditional, which is not suitable for large-scale production of cultivated products. Therefore, it is urgent to find a scientific, reasonable and efficient processing methods. Aimed at providing references for standardization production of Fritillaria, this paper made a textual research on the ancient and modern herbal literature, the Chinese Pharmacopoeia and other medicinal standards, combined with modern literature, the harvesting and processing methods of Fritillaria were sorted out and prospected.
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ObjectiveTo analyze the production of Poria in 5 major producing areas and the problems through field investigation and literature review and thus to lay a foundation for healthy development of Poria industry. MethodEach production process of Poria in Anhui, Hubei, Hunan, Yunnan, and Sichuan was probed and related research was retrieved for the analysis. ResultSichuan, Guangxi, Guizhou, and other areas have been the emerging producers of Poria. However, a few Poria cocos lines were used and the variety breeding lagged behind. Different cultivation techniques were adopted in different production areas. For example, the "induction" method failed to be widely used. Moreover, the harvest time was mainly dependent on the market demand not matter it was suitable or not. Furthermore, steaming has replaced the traditional diaphoretic processing in the processing of this medicinal material in production areas. In addition, fumigation with sulphur was still used in the processing of Poria. ConclusionAn excellent variety is the key to the quality of Poria. Efforts should be made to strengthen the evaluation of the germplasm resources of P. cocos and variety breeding and standardize the processing in production areas, thereby ensuring the safety and effectiveness of Poria and the decoction pieces. In addition, the contradiction between artificial cultivation and woodland ecology should be fully coordinated to ensure the sustainable development of Poria industry in China.
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Objective:To evaluate the prognostic value of plasma microRNA-30b-5p (miR-30b-5p) combined with extravascular lung water index (EVLWI) in patients with acute respiratory distress syndrome (ARDS).Methods:120 ARDS patients admitted to Danzhou People's Hospital from January 2016 to June 2019 were enrolled. The gender, age, body mass index (BMI), underlying diseases, etiology and baseline values of heart rate (HR), respiratory rate (RR), oxygenation index (OI), arterial partial pressure of carbon dioxide (PaCO 2) and acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score were collected. According to the survival outcome during hospitalization, the patients were divided into survival group and death group. According to OI, the patients were divided into mild-moderate group (OI > 100 mmHg, 1 mmHg = 0.133 kPa) and severe group (OI ≤ 100 mmHg). The expression of plasma miR-30b-5p was detected by real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR), and EVLWI was measured. The receiver operating characteristic (ROC) curve was drawn to analyze the value of plasma miR-30b-5p and EVLWI in predicting the death of patients with ARDS. Pearson correlation method was used to analyze the correlation between miR-30b-5p and EVLWI in ARDS patients with different prognosis during hospitalization. Results:120 patients with ARDS were enrolled in the analysis, with 42 patients in the death group, and 78 in the survival group; with 67 patients in the mild-moderate group, and 53 in the severe group. APACHE Ⅱ score in the death group was higher than that in the survival group, but there was no significant difference in gender, age, BMI, underlying diseases, etiology or baseline values of HR, RR, OI, or PaCO 2 between the two groups. The expression of plasma miR-30b-5p and EVLWI level in the death group were significantly higher than those in the survival group [miR-30b-5p (2 -ΔΔCt): 2.28±0.74 vs. 0.52±0.06, EVLWI (mL/kg): 15.38±4.60 vs. 10.24±2.15, both P < 0.01]. The expression of plasma miR-30b-5p, EVLWI and mortality during hospitalization in the severe group were significantly higher than those in the mild-moderate group [miR-30b-5p (2 -ΔΔCt): 2.05±0.65 vs. 0.93±0.17, EVLWI (mL/kg): 14.65±4.20 vs. 11.36±2.28, mortality during hospitalization: 58.5% (31/53) vs. 16.4% (11/67), all P < 0.01]. ROC curve analysis showed that the best cut-off value of plasma miR-30b-5p and EVLWI in predicting the death during hospitalization of ARDS patients were 1.62 and 13.28 mL/kg, respectively. Moreover, the area under ROC curve (AUC) of the combination of two parameters was significantly higher than that of the two alone (0.897 vs. 0.827, 0.785), with high sensitivity and specificity, 90.5% and 84.2%, respectively. Pearson correlation analysis showed that plasma miR-30b-5p in dead ARDS patients was significantly positively correlated with EVLWI ( r = 0.768, P < 0.01), but the correlation was not found in surviving patients ( r = 0.118, P > 0.05). Conclusion:The expression of plasma miR-30b-5p and EVLWI are related to the severity and prognosis of patients with ARDS, and the combination of the two has certain evaluation value for the prognosis of ARDS patients.
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OBJECTIVE:To establish the quality standard of the Tibetan medicine Ma lus Fructus (known as the fruit of Ese ), and to provide scientific reference for quality control and utilization of resource. METHODS :The qualitative analysis of 20 batches of Malus Fructus with different producing areas and different origins was carried out by morphological observation ,microscopic and TLC identification. According to the method stated in 2015 edition of Chinese Pharmacopoeia ,water content ,total ash content and extract content were determined. HPLC method was used to determine the contents of 6 kinds of flavones (chlorogenic acid , hyperin,isoquercitrin,quercitin,phloridzin,quercetin)in Malus Fructus. RESULTS :The properties of this medicinal material were obovate ,spheroidal or oval ,its surface was reddish brown to dark red ,with slight shrinkage. Its taste was sweet ,sour and slightly astringent. The microscopic characteristics of the powder were clear ,the stone cells were scattered individually or clustered,and the pulp parenchyma cells contained calcium oxalate cube crystal and cluster crystal. The results of TLC showed that there were fluorescent spots of the same color in the chromatogram of the tested product and the corresponding position of the reference substance (ursolic acid ). The content ranges of moisture ,total ash and extracts were 9.02%-11.39%,2.29%-3.35%, 37.12%-58.41% respectively. The linear ranges of chlorogenic acid ,hyperin,isoquercitrin,,quercetin phlorizin and quercetin were 3.12-50,1.25-20,1.25-20,3.12-50,18.7-300,0.5-8 μg/mL(all r were not less than 0.999 7). RSDs of prescision ,stability and reproducibility tests were all lower than 3.0%(n=6). The average recoveries were 97.56%,98.28%,106.14%,103.74%, 98.37%,98.76%;RSDs were 1.57%,1.62%,1.38%,1.99%,2.15%,2.31%(n=6). The content ranges of the above six components in 20 baches of samples were 0.001 5%-0.028 8%,0.005 9%-0.022 0%,0.008 7%-0.030 4%,0.019 4%- 0.048 5%,0.068 5%-0.199 8% and 0.005 1%-0.081 4%. CONCLUSIONS :Established method is accurate and stable ,and can be used for quality control of Malus Fructus. It is preliminarily Δ 基金项目:四川省科技计划项目(No.2020YFS0495);“杏林学 proposed that moisture and total ash content should not exceed 者”学科人才科研提升计划(No.CXTD2018016) 12% and 3.5%,and extract (by dry product )and phlorizin *硕士研究生 。研究方向 :中药品种 、质量与资源 。E-mail: content should not be less than 40% and 0.1% respectively. 1159020313@qq.com # 通信作者:教授。研究方向:中药品种、质量与资源。E-mail:
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Objective·To investigate the effect of early neurodynamic mobilization on the recurrence of sciatica after the minimally invasive surgery-transforaminal lumbar interbody fusion (MIS-TLIF). Methods·Fifty-four patients who underwent MIS-TLIF were divided into treatment group (n=28) and control group (n=26). The control group received conventional rehabilitation treatment, while the treatment group received neurodynamic mobilization every day after the first post-operative day. The score of visual analogue scale (VAS) and Japanese Orthopaedic Association (JOA) score were evaluated before and after the treatment. Results·There was no significant difference in the score of VAS or JOA between two groups before the treatment or three months after surgery (P>0.05). The score of VAS was significantly lower (P<0.05), and the JOA score was significantly higher (P<0.05) in the treatment group than in the control group four days after surgery. Conclusion·The early neurodynamic mobilization is effective to decrease the recurrence rate of sciatica in patients underwent MIS-TLIF.