Isolation and characterization of phosphate-solubilizing bacteria from rhizosphere of poplar on road verge and their antagonistic potential against various phytopathogens.

BACKGROUND: Phosphate-solubilizing bacteria (PSB) can solubilize insoluble phosphate compounds and improve phosphate availability in soil. Road verges are important in urban landscaping, but the population structure of PSB and their ecological functions in the road verge soil is still unclear. RESULTS: Twenty-one mineral PSB strains and 14 organic PSB strains were isolated from the rhizosphere of poplar on urban road verge. All the mineral PSB strains showed better solubilization to Ca3(PO4)2 than FePO4 or AlPO4. Among them, 7 strains showed high phosphate-solubilizing (PS) activities to Ca3(PO4)2 (150-453 mg/L). All the organic PSB strains displayed weak solubilization to lecithin. 16S rRNA gene-based phylogenetic analysis showed good species diversity of the PSB strains, which belongs to 12 genera: Bacillus, Cedecea, Cellulosimicrobium, Delftia, Ensifer, Paenibacillus, Pantoea, Phyllobacterium, Pseudomonas, Rhizobium, Sinorhizobium and Staphylococcus. Moreover, 8 PSB strains showed various degrees of growth inhibition against 4 plant pathogenic fungi, Fusarium oxysporum S1, F. oxysporum S2, Pythium deliense Meurs Z4, Phomopsis sp. AC1 and a plant pathogenic bacterium, Pectobacterium carotovorum TP1. CONCLUSIONS: The results indicated that these PSB strains could perform multiple ecological functions on road verge. The development and application of bio-agents based on the strains would provide a new strategy for maintaining and improving the ecosystem stability of road verges.

Characterization of phosphorus accumulation and release using diffusive gradients in thin films (DGT) - linking the watershed to Taihu Lake, China.

Taihu Lake is well known internationally due to its algae bloom, and phosphorus fertilization and accumulation in the watershed soil played an important role in the lake eutrophication. To explore the linkage between soil and sediment legacy P, the soils from four representative land uses in the upstream watershed and sediments from typical lake areas were sampled and analyzed for P species. Meanwhile, the DGT technology was used to characterize the labile P in the soil and sediment and its release dynamics. The results indicated that Taihu Lake was the major total phosphorus sink (TP = 481.7 ±â€¯97 mg/kg) and wheat land the major reactive phosphorus stock (RP = 37.3 ±â€¯9 mg/kg) in the watershed. The DGT-P dynamic with the deployment time (t) presented a downward exponential curve (f(t) = a × t-b, b > 0) and the regression parameters implied the initial P stock (a) and release rate (b) of labile P. Although the result of the extract based method was statistically correlated to the DGT measured P (p < 0.05), the DGT result had advantages over the traditional method due to its in-situ measurement and kinetic characterization of available P. Since the regression parameters reflected combined factors that impact the interaction between solid and solution P, the soil or sediment properties such as pH and organic matters need to be further studied in details, although they presented a significant correlation with DGT-P in this work. Our work provides a new insight in the rapid assessment of the size and resupply capacity of the existing legacy P stocks.

Global research hotspots on oncology-bibliometric analysis based on the ESI hot papers / 生物医学工程学杂志

With the increasing global burden of various cancer, an abundance of papers emerged every year in the research hotspots of oncology, covering a wide range of research types and topics. In order to facilitate interested readers to quickly grasp the frontier and hotspots of cancer research, it would be helpful to sort out and summarize the research topic in a timely manner. According to the classification of disciplines, we screened the Essential Science Indicators (ESI) hot papers released in 2019 for the ones in the oncology field, utilized methods such as bibliometrics, statistical description, hierarchical induction, analysis and interpretation to further reveal the context and characteristics of research in the field of oncology, summarized the latest progresses and future directions in the field, and provided information and hints for the trajectory of future research. A total of 549 papers were included, which were mainly from the field of clinical medicine; the country with the most publications was the United States, while China ranked the fourth in terms of contribution; the research institution with the highest number of published papers was University of Texas system;

Research progress of the relationship between 14-3-3ζ and malignant tumor / 实用肿瘤学杂志

The 14-3-3 protein is a highly conserved acidic polypeptide family involved in intracellular signaling,protein transportation, cell proliferation, invasion, migration, and apoptosis. Many studies have shown that 14-3-3ζhas a high expression level in many malignant tumors. This paper introduces the structure of 14-3-3ζ,the biological function,and the development of the malignant tumor ( breast cancer,lung cancer,hepatocel-lular carcinoma,glioma,head and neck neoplasms) related to the development of 14-3-3ζand treatment of ma-lignant tumor in order to provide valuable information and ideas.

Effect of Shunaoxin dropping pills on neurocognitive function of patients with vascular cognitive impairment of none dementia / 中华神经医学杂志

Objective To evaluate the effect of Shunaoxin dropping pills on neuropsychological features of patients with vascular cognitive impairment of none dementia.Methods One hundred and three outpatients with vascular cognitive impairment of none dementia,collected from November 2014 to January 2015,were enrolled in our study;they were divided into two groups:Shunaoxin treatment group (n=50) received Shunaoxin pills and conventional therapy and control group (n=53) received conventional therapy;conventional therapy included anti-platelet therapy (clopidogrel 75 mg once daily),control of pressure (target value=140/90 mmHg) and control of blood fat (atorvastatin 20 mg once daily).Shunaoxin pills (four pills/time for three times per day) were taken orally by the patients from the Shunaoxin treatment group for consecutive three months.Their neuropsychological features were evaluated by Montreal cognitive assessment (MoCA),digital span test (DST),auditory verbal learning test (AVLT),trail making test (TMT),verbal fluency test (VFT) and Stroop test before treatment and 3 months after treatment.Results There were 47 available cases in both two groups,respectively.As compared with control group,Shunaoxin treatment group had significantly increased MoCA total scores,scores of MoCA-language and MoCA-delayed recall,and VLT scores (learning,recall and recognition,P＜0.05),while there were no significant changes in the rest parts of MoCA,TMT,VFT and Stroop test (P＞0.05).Conclusion Based on the therapy of anti-platelet,control of pressure and blood fat,3 months therapy of Shunaoxin dropping pills can improve general cognition,especially in verbal learning,delayed recall and verbal repeat.

Advances in studies on intestinal absorption kinetics of phenolic glycosides / 中国生化药物杂志

This review analyzed domestic and foreign literatures on intestinal absorption,introduced the current methods and models commonly used in phenolic glycosides researches and their impacts focused on intestinal absorption,summarized the research status,aims to provide a reference for improving their oral bioavailability,improve formulations,new medicine and clinical rational use of phenolic glycosides.