RESUMO
Aim: To explore the preventive effect of water extract of Danshen (DWE) on degeneration of osteoarticular cartilage induced by monosodium iodocaetate (MIA). Methods: Twenty-seven female SD ratswere randomly divided into sham group, MIA group and DWE group. Rats in sham group were injected a single intra-articular of saline in left knee, while rats in MIA and DWE group were injected a single intra-articular of MIA(60 g L-1). The rats were administered on the second day after the injection of MIA. The rats in sham group and MIA group were given 5 mL · kg-1 of saline, and the rats in DWE group received 5 mL · kg-1 of DWE every day for six weeks. At the end of the experiment, the serum was tested for MMP-13, IL- 6 and TNF-α by enzyme-linked immunosorbent assay (ELISA). The left knee femur of rats was taken for safranine O-fixed green staining and tibia was collected for Micro-CT assay. Results: Compared with sham group, the serum level of MMP-13 and the pathological score of the medial femoral articular cartilage in MIA group significantly increased (P < 0. 01), while cartilage thickness and cartilage volume of the medial tibial articular cartilage markedly decreased (P < 0. 01). Compared with MIA group, the serum level of MMP-13 and the pathological score of the medial femoral articular cartilage in DWE group significantly decreased (P <0. 05,P <0. 01), while cartilage thickness and cartilage volume of the medial tibial articular cartilage increased (P < 0. 01, P < 0. 05). Conclusion: DWE has a protective effect on degeneration of osteoarticular cartilage induced by monosodium iodocaetate.
RESUMO
OBJECTIVES@#To investigate the genetic polymorphism of DYS391 and other 23 Y-STR loci and to explore its application value in forensic science.@*METHODS@#Y-STRs loci of 580 unrelated Han males in Nanjing were amplified using AGCU Y-PLUS PCR （24） kit. The genetic parameters of 24 Y-STR loci such as gene frequency were calculated by software, and compared with the data of Hubei, Liao- ning, Guangdong, Beijing and Chengdu Han population.@*RESULTS@#Total 580 haplotypes were detected among 24 Y-STR loci in 580 unrelated Han males in Nanjing. The genetic diversity （GD） of each locus was from 0.294 6 to 0.939 8, and the haplotypes diversity （HD） was 0.983 7. There was a significant difference between the GD of 6 areas.@*CONCLUSIONS@#The 24 Y-STR loci such as DYS391 in Nanjing Han population have an application value in forensic science. They can also be used for cases testing and pedigree investigation.