The Value of the Apolipoprotein B/Apolipoprotein A1 Ratio in Predicting the Rapid Progression of Non-Culprit Coronary Lesions in Acute Coronary Syndrome in Patients with Diabetes Mellitus after Percutaneous Coronary Intervention.

This study aims to assess the predictive value of the apolipoprotein B (ApoB) /apolipoprotein A1 (ApoA1) ratio in acute coronary syndrome (ACS) in patients with diabetes mellitus (DM) for the rapid progression (RP) of non-culprit coronary lesions (NCCLs) after percutaneous coronary intervention (PCI) and observe the effect of the ApoB/ApoA1 ratio on major adverse cardiac events (MACE).A total of 175 patients with DM presenting with ACS who received a PCI and an average 13-month follow-up coronary angiography (CAG) were enrolled from January 2015 to December 2020. According to the CAG, the patients were divided into the RP group and the non-RP group. MACE was defined as a composite of death from cardiac causes, cardiac arrest, myocardial infarction, or rehospitalization from unstable or progressive angina at the end of a 24-month follow-up.The low-density lipoprotein cholesterol (LDL-C), ApoB, ApoB/ApoA1 ratio, and LDL-C/high-density lipoprotein cholesterol (HDL-C) ratio levels at baseline were significantly higher in the RP group than in the non-RP group. The ApoA1 level at baseline in the non-RP group was significantly higher than in the RP group. The predictive significance of the ApoB/ApoA1 ratio (area under the curve (AUC) = 0.712) for the RP of NCCLs was significantly higher than those of ApoA1, ApoB, LDL-C/HDL-C ratio (AUC = 0.628, AUC = 0.640, and AUC = 0.620, respectively). A higher ApoB/ApoA1 ratio and the RP of NCCLs were significantly associated with the occurrence of MACE.The ApoB/ApoA1 ratio was an effective clinical indicator for the RP of NCCLs after PCI in patients with DM presenting with ACS. The high ApoB/ApoA1 ratio and the RP of NCCLs were two risks for MACE.

Study on the Hyperspectral Retrieval and Ecological Risk Assessment of Soil Cr, Ni, Zn Heavy Metals in Tailings Area.

The large-scale rapid monitoring of heavy metal pollution has become a hot topic due to increasing contamination of Tailings soil by heavy metal. In order to explore the possibility of using soil spectrum to estimate the content of heavy metals in soil and realize the rapid monitoring of soil heavy metals in the Yangshanchong tailings area in Tongling, China. The spectral reflectance of soil and the content of heavy metals (Cr, Ni, Zn) in soil were determined. The optimal bands of Cr, Ni and Zn elements in soil appeared at 467 nm, 467 nm and 468 nm respectively, and the maximum correlation coefficients were - 0.716, - 0.685 and - 0.630. The inversion model of element Cr constructed under the Reciprocal Transformation Second Derivative has a better effect, and its determination coefficient R2 is 0.613; It is better to construct the model of elements Ni and Zn in the form of Reciprocal Transformation First Derivative, and their determination coefficients R2 are 0.724 and 0.603, respectively. The results of the single factor index method showed that the pollution degree of heavy metal elements in the soil in the study area is Ni > Zn > Cr; the Nemerow comprehensive pollution index method showed that the three elements in the study area were polluted to varying degrees, and the comprehensive pollution index was in order Ni > Zn > Cr; Comprehensive potential ecological hazard index evaluation, the pollution degree and ecological risk of the study area were low.

Molecular epidemiology, antimicrobial activity, and virulence gene clustering of Streptococcus agalactiae isolated from dairy cattle with mastitis in China.

Streptococcus agalactiae is a contagious pathogen that causes bovine mastitis worldwide, resulting in considerable economic losses. In this study, we isolated 42 S. agalactiae strains in 379 milk samples from cows with subclinical mastitis on 15 dairy farms in 12 Chinese provinces. Analysis based on capsular typing and multilocus sequence typing, combined with patterns of virulence gene scanning and antimicrobial resistance, identified the lineages and populations of the isolates. We grouped the 42 isolates into 7 sequence types belonging to 6 clonal complexes, mainly CC103 (31/42 isolates; 73.8%). We identified an ST-23 strain named Sa 129 for the first time on Chinese dairy farms-this strain is usually associated with human isolates. Capsular types Ia and II were predominant in capsular typing. The prevalence of virulence profile 1 (bibA, cfb, cspA, cylE, fbsA, fbsB, hylB, and pavA) was 64.3%, and represented the main trend in China. With respect to antimicrobial resistance, most isolates were susceptible to ß-lactams, rifamycin, glycopeptides, and oxazolidone; resistance to several antimicrobial agents, including lincomycin, clindamycin, and doxycycline, varied in 4 different regions. Our research provides a profile for the molecular epidemiology, multilocus sequence typing, antimicrobial resistance, and virulence gene clustering of S. agalactiae, and may be beneficial for the clinical monitoring, prevention, and control of mastitis in dairy cattle.

An examination of eating and thinness expectancies as predictors of eating disturbances in females: evidence for cultural differences between European American and Chinese college students.

PURPOSE: This study sought to examine the utility of eating expectancy, thinness expectancy, and the interactive role of both, in predicting eating disturbances (viz., bulimic symptoms and drive for thinness) in European American and Chinese female college students. METHODS: A sample of 237 European American and 221 Chinese female college students completed measures of eating and thinness expectancies and eating disturbances. RESULTS: Results of hierarchical regression analyses revealed that thinness expectancy significantly predicted increases in both drive for thinness and bulimic symptoms (with these increases being greater for European Americans), whereas eating expectancy predicted increases in bulimic symptoms only. In addition, for European Americans, a significant interaction for bulimic symptoms was found, revealing a synergistic increase in bulimic symptoms for those with both a high thinness expectancy and a high eating expectancy. For Chinese, a significant interaction for drive for thinness was found, demonstrating that for those with a high thinness expectancy, a higher eating expectancy was actually associated with a lower drive for thinness. CONCLUSION: The present findings point to the value of examining for the co-presence of both expectancies in predicting eating disturbances while also highlighting cultural variations in the study of eating pathology. LEVEL OF EVIDENCE: Descriptive cross-sectional study, level V.

Comparative study on effects of four energy plants growth on chemical fractions of heavy metals and activity of soil enzymes in copper mine tailings.

Four gramineous energy plants, Miscanthus sacchariflorus, M. floridulus, Phragmites australis, and Arundo donax were grown on copper tailings in the field for four years. Their phytoremediation potential was examined in terms of their effects on the fractions of heavy metals and soil enzyme activities. Results showed that plantation of these four gramineous plants has improved the proportion of organic material (OM)-binding fraction of heavy metals in copper tailings as a whole, and reduced the proportion of exchangeable and residual fractions. In particular, M. sacchariflorus growth improved significantly the proportion of the OM-binding fractions of Cu (1.73 times), Cd (1.71 times), Zn (1.18 times), and Pb (3.14 times) (P < 0.05) and reduced markedly the residual fractions of Cu (64.45%), Cd (82.38%), Zn (61.43%), and Pb (73.41%) (P < 0.05). Except for A. donax, the growth of other three energy plants improved the activity of phosphatase, urease and dehydrogenase in copper tailings to some extent. In particular, the activity of soil phosphatase and urease in planted tailings differed significantly from that of control (P < 0.05). The effect of M. sacchariflorus growth on soil enzyme was the highest, followed by P. australis, M. floridulus, and A. donax. The content of each heavy metal fraction in soil was correlated with soil enzyme activities, especially the content of OM-binding fraction, which correlated significantly with the activities of phosphatase, urease and dehydrogenase in soil. According to the effects of four gramineous plants growth on activity of soil enzymes and fractions of heavy metals, M. sacchariflorus had the optimal effects for phytoremediation. Therefore, M. sacchariflorus was a candidate plant with great potential for the revegetation of heavy metal tailings.

Identification Study of Edible Oil Species with Laser Induced Fluorescence Technology Based on Liquid Core Optical Fiber.

A laser- induced fluorescence detection set based on liquid core optical fiber was established in this study. Eight edible oils were discriminated by using this detection set combined with chemometrics method. The effect of length of liquid core optical fiber on laser induced fluorescence spectrum was explored, and the differences between the spectra of different edible oils were analyzed. The fluorescence spectra of 320 samples covering 8 types of edible oil were measured in 1 meter liquid core optical fiber. Principal component analysis was used in fluorescence data dimensionality reduction process. Partial least squares discriminant analysis (PLS-DA) method was used to develop the identification model to distinguish edible oil species. The results showed that the oil fluorescence intensity is greatly enhanced when liquid core optical fiber was used. With the increase of liquid core optical fiber length，the peaks of laser induced edible oil fluorescence spectra increased and the fluorescence spectra will produce red shift. The red shift tended to a constant value when the fiber length was more than 80 cm. The fluorescence spectra of different edible oils were quite different, its can be used to distinguish different types of edible oil. Principal component scores chart were get using PC1 and PC2 of edible oils fluorescence data which result in a trend of certain gather of same type of edible oil. The recognition rates of PLS-DA model for the calibration set and prediction set were both 100%. The study shows that the developed device in this study has high accuracy for identifying the edible oil species.

Examining the structure, reliability, and validity of the Chinese personal growth initiative scale-II: evidence for the importance of intentional self-change among Chinese.

We examined the factor structure, reliability, and validity of the Chinese version of the Personal Growth Initiative Scale-II (CPGIS-II) using data from a sample of 927 Chinese university students. Consistent with previous findings, confirmatory factor analyses supported a 4-factor model of the CPGIS-II. Reliability analyses indicated that the 4 CPGIS-II subscales, namely Readiness for Change, Planfulness, Using Resources, and Intentional Behavior, demonstrated good internal consistency reliability and adequate test-retest reliability across a 4-week period. In addition, evidence for convergent and incremental validity was found in relation to measures of positive and negative psychological adjustment. Finally, results of hierarchical regression analyses indicated that the 4 personal growth initiative dimensions, especially planfulness, accounted for additional unique variance in psychological adjustment beyond resilience. Some implications for using the CPGIS-II in Chinese are discussed.

Chinese version of the Responses to Positive Affect Questionnaire: testing the factor structure, reliability, and validity in a college student sample.

This study tested the psychometrics of the Chinese version of the Responses to Positive Affect Questionnaire among 915 Chinese college students with an average age of 20.3 yr. (SD = 1.6). The original three-factor model with the factors dampening, emotion-focused positive rumination, and self-focused rumination was supported using exploratory and confirmatory factor analyses. All subscales showed good internal reliability, as well as evidence for convergent and incremental validity with measures of ego-resiliency, life satisfaction, and mental health symptoms. Finally, a series of hierarchical regression analyses indicated that the three subscales, especially dampening, accounted for additional unique variance in psychological adjustment above and beyond resilience. These findings generally suggested that the Chinese Responses to Positive Affect Questionnaire possesses acceptable psychometric properties. Implications for counseling, limitations, and suggestions for future study were presented.

Rapid immunoassays for the detection of quinoxalines and their metabolites residues in animal-derived foods: A review.

Quinoxalines are a class of veterinary drugs with antibacterial and growth-promoting functions. They are often widely used to treat and prevent animal diseases and are illegally used as animal growth promoters to increase economic benefits. Quinoxalines could be easily metabolized in animals to various residue markers and remain in animal-derived foods, which would pose a serious threat to human health. Consequently, it is necessary to detect the residues of quinoxalines and their metabolites. This article reviewed and evaluated immunoassays for quinoxalines and their metabolites in animal-derived foods, mainly including enzyme-linked immunosorbent assays, fluorescence immunosorbent assays, immunochromatography, and surface plasmon resonance biosensors. In addition, we deeply explored the design of haptens for quinoxalines and their metabolites and analyzed the effect of haptens on antibody performance. This paper aims to provide guidance and references for their accurate and sensitive detection, thereby ensuring food safety and human public health.

Antiviral activity of flavonol against porcine epidemic diarrhea virus.

Porcine epidemic diarrhea virus (PEDV) remains one of the major causative microorganisms of viral diarrhea in piglets worldwide, with no approved drugs for treatment. We identified a natural molecule, flavonol, which is widely found in tea, vegetables and herbs. Subsequently, the antiviral activity of compound flavonol was evaluated in Vero cells and IPEC-J2 cells, and its anti-PEDV mechanism was analyzed by molecular docking and molecular dynamics. The results showed that flavonol could effectively inhibit viral progeny production, RNA synthesis and protein expression of PEDV strains in a dose-dependent manner. When flavonol was added simultaneously with viral infection in Vero cells, it demonstrated potent anti-PEDV activity by affecting the viral attachment and internalization phases. Similarly, in IPEC-J2 cells, flavonol effectively inhibited PEDV infection at different stages of infection, except for the release phase. Moreover, flavonol mainly interacts with PEDV Mpro through hydrogen bonds and hydrophobic forces, and the complex formed by it has high stability. Importantly, flavonol also showed broad-spectrum activity against other porcine enteric coronaviruses such as TGEV and PDCoV in vitro. These findings suggest that flavonol may exert antiviral effects by interacting with viral Mpro, thereby affecting viral replication. This means that flavonol is expected to become a potential drug to prevent or treat porcine enteric coronavirus.

Preparation of high-affinity and high-specificity monoclonal antibody and development of ultra-sensitive immunoassay for the detection of 1-aminohydantoin, the metabolite of nitrofurantoin.

1-Aminohydantoin (AHD), the residual marker of nitrofurantoin, is usually detected after derivatisation using the derivatisation reagent 2-nitrobenzaldehyde. Avoiding the antibody recognition of the derivatisation reagent is essential for the accurate detection of AHD residues. In this paper, a novel hapten called hapten D was designed, and then, a monoclonal antibody that did not recognise 2-nitrobenzaldehyde was prepared based on this novel hapten. An ultra-sensitive indirect competitive enzyme linked-immunosorbent assay (icELISA) was established under optimal conditions. The 50% inhibition concentration and limit of detection of AHD were 0.056 and 0.0060 ng/mL, respectively, which improved the sensitivity by 9-37-fold compared with the previously reported icELISA methods. The average recovery rates were 88.1%-97.3%, and the coefficient of variation was <8.6%. The accuracy and reliability of the icELISA were verified using liquid chromatography-tandem mass spectrometry. These results demonstrated that the developed icELISA is a useful and reliable tool.

Predictions of soil organic carbon using laboratory-based hyperspectral data in the northern Tianshan mountains, China.

This paper presents a study dealing with soil organic carbon (SOC) estimation of soil through the combination of soil spectroscopy and multivariate stepwise linear regression. Soil samples were collected in the three sub-regions, dominated by brown calcic soil, in the northern Tianshan Mountains, China. Spectral measurements for all soil samples were performed in a controlled laboratory environment by a portable ASD FieldSpec FR spectrometer (350-2,500 nm). Twelve types of transformations were applied to the soil reflectance to remove the noise and to linearize the correlation between reflectance and SOC content. Based on the spectral reflectance and its derivatives, hyperspectral models can be built using correlation analysis and multivariable statistical methods. The results show that the main response range of soil organic carbon is between 400 and 750 nm. Correlation analysis indicated that SOC has stronger correlation with the second derivative than with the original reflectance and other transformations data. The two models developed with laboratory spectra gave good predictions of SOC, with root mean square error (RMSE) <5.0. The use of the full visible near-infrared spectral range gave better SOC predictions than using visible separately. The multivariate stepwise linear regression of second derivate model (model A) is optimal for estimating SOC content, with a determination coefficient of 0.894 and RMSE of 0.322. The results of this research study indicated that, for the grassland regions, combining soil spectroscopy and mathematical statistical methods does favor accurate prediction of SOC.

Development and Initial Validation of the Positive and Negative Co-Rumination Scale.

This article presents two studies with data from 750 college students (58.67% females, Mage = 20.79 years) and 1035 school students (52.1% girls, Mage = 14.44 years) respectively, describing the development and initial validation of the Positive and Negative Co-Rumination Scale (PANCRS). The PANCRS consists of 32 items with 3 second-order factors: Positive Co-Rumination consisting of 3 first-order factors (i.e., Affirmation, Problem-Solving and Enhancing Friendship), Negative Co-Rumination consisting of 4 first-order factors (i.e., Worry About Evaluation, Inhibiting Happiness, Worry About Impact and Slack) and Frequency consisting of 2 first-order factors (i.e., Frequencies of Co-Rumination on Positive and Negative Events). Results from exploratory and confirmatory factor analyses confirmed the measure's 9 first-order and 3 second-order factors structure. Moreover, correlation analyses provided first evidence for the subscales' differential validity: (1) Positive Co-Rumination showed positive correlations with positive indicators of psychological adjustment (i.e., friendship quality and life satisfaction) and negative correlations with negative indicators of psychological adjustment (i.e., anxiety and depression); (2) Negative Co-Rumination showed non-significant or negative correlations with positive indicators of psychological adjustment and positive correlations with negative indicators of psychological adjustment; (3) Frequency showed positive correlations with both positive and negative indicators of psychological adjustment. In addition, all PANCRS scores showed satisfactory composite reliability (omegas) and temporal stability (test-retest). Overall the findings suggest that the PANCRS is a reliable and valid instrument to assess positive and negative aspects of Co-rumination.

A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment.

Pretilachlor is a chloroacetamide herbicide mainly used for weed and broadleaf weed control in rice, that is widely utilized in China. In order to detect the residue of pretilachlor in the environment and food, a highly sensitive and specific monoclonal antibody (mAb) against pretilachlor was prepared, and the half maximum inhibitory concentration (IC50) of the monoclonal antibody was validated to be 31.47 ± 2.35 µg/L. An indirect competitive ELISA (ic-ELISA) based on the antibody with a linear range of 6.25~100 µg/L was developed. The specificity of the antibody was explained by computer simulations and experimental validation. The mAb exhibited negligible cross-reactivity towards alachlor, acetochlor, propisochlor, butachlor, and metalaxyl, and the limits of detection (LOD) for pretilachlor in lake, rice, and soil samples were 4.83~5.23 µg/L. The recoveries of all samples were 78.3~91.3%. The reliability of the ic-ELISA method for residue detection of pretilachlor in the environment and grains was confirmed using high performance liquid chromatography.

FCSN: Global Context Aware Segmentation by Learning the Fourier Coefficients of Objects in Medical Images.

The encoder-decoder model is a commonly used Deep Neural Network (DNN) model for medical image segmentation. Conventional encoder-decoder models make pixel-wise predictions focusing heavily on local patterns around the pixel. This makes it challenging to give segmentation that preserves the object's shape and topology, which often requires an understanding of the global context. In this work, we propose a Fourier Coefficient Segmentation Network (FCSN)-a novel global context-aware DNN model that segments an object by learning the complex Fourier coefficients of the object's masks. The Fourier coefficients are calculated by integrating over the whole contour. Therefore, for our model to make a precise estimation of the coefficients, the model is motivated to incorporate the global context of the object, leading to a more accurate segmentation of the object's shape. This global context awareness also makes our model robust to unseen local perturbations during inference, such as additive noise or motion blur that are prevalent in medical images. We compare FCSN with other state-of-the-art global context-aware models (UNet++, DeepLabV3+, UNETR) on 5 medical image segmentation tasks, of which 3 are camera imaging datasets (ISIC_2018, RIM_CUP, RIM_DISC) and 2 are medical imaging datasets (PROSTATE, FETAL). When FCSN is compared with UNETR, FCSN attains significantly lower Hausdorff scores with 19.14 (6%), 17.42 (6%), 9.16 (14%), 11.18 (22%), and 5.98 (6%) for ISIC_2018, RIM_CUP, RIM_DISC, PROSTATE, and FETAL tasks respectively. Moreover, FCSN is lightweight by discarding the decoder module, which incurs significant computational overhead. FCSN only requires 29.7 M parameters which are 75.6 M and 9.9 M fewer parameters than UNETR and DeepLabV3+, respectively. FCSN attains inference and training speeds of 1.6 ms/img and 6.3 ms/img, which is 8× and 3× faster than UNet and UNETR. The code for FCSN is made publicly available at https://github.com/nus-mornin-lab/FCSN.

Preparation of Monoclonal Antibody against Pyrene and Benzo [a]pyrene and Development of Enzyme-Linked Immunosorbent Assay for Fish, Shrimp and Crab Samples.

Polycyclic aromatic hydrocarbons (PAHs) are significant environmental and food pollutants that can cause cancer. In this work, a specific monoclonal antibody (mAb) to identify pyrene (PYR) and benzo [a]pyrene (BaP) was prepared, and an indirect competitive enzyme-linked immunoassay (ic-ELISA) was established to detect PYR and BaP residues in living aquatic products for the first time. The effects of complete antigens with different coupling ratios on the production of high-sensitivity mAb was explored. Under the optimal conditions, the IC50 value was 3.73 ± 0.43 µg/L (n = 5). The limits of detection (LODs) for PYR and BaP in fish, shrimp, and crab ranged from 0.43 to 0.98 µg/L. The average recoveries of the spiked samples ranged from 81.5-101.9%, and the coefficient of variation (CV) was less than 11.7%. The validation of the HPLC-FLD method indicated that the ELISA method set up in this experiment provided a trustworthy tool for PAHs residues detection in aquatic products.

Training Positive Rumination in Expressive Writing to Enhance Psychological Adjustment and Working Memory Updating for Maladaptive Ruminators.

Rumination is associated with psychological adjustment and working memory (WM) capacity. Studies have shown that psychological interventions can reduce negative rumination and improve psychological adjustment and WM capacity. The present study investigated the effect of positive rumination training in expressive writing on psychological adjustment and WM updating capacity. Within an experimental design, positive rumination was manipulated for 10 participants who were maladaptive ruminators in an experiment using a 5-week training compared to the control group with nine participants. Results revealed significant enhancement of psychological adjustment and the response time (RT) of WM updating in the experimental group but not in the control group. The two groups did not show significant difference of all the variables in pretest. However, the experimental group showed significantly better outcomes than the control group in posttest. The results suggest that positive rumination training in expressive writing is effective and rumination has a causal influence on WM updating capacity.

Corrigendum: Training Positive Rumination in Expressive Writing to Enhance Psychological Adjustment and Working Memory Updating for Maladaptive Ruminators.

[This corrects the article DOI: 10.3389/fpsyg.2020.00789.].

Assessing the spatiotemporal variation of NPP and its response to driving factors in Anhui province, China.

Net primary productivity (NPP) of terrestrial ecosystems is an important metric of ecosystem functioning; however, the understanding of response mechanism of NPP to influencing factors and driving mechanisms are still limited. In this study, taking Anhui province as an example, spatio-temporal changes of NPP and its response to influencing factors were investigated for evaluating the effects of climate change and land use and land cover change (LUCC) on regional NPP. The Carnegie-Ames-Stanford Approach (CASA) model was employed for NPP simulation by using the MODIS normalized difference vegetation index (NDVI) data and meteorological data over 2001-2016. Combined domestic LUCC, the spatiotemporal distribution pattern and dynamic change characteristics of NPP under a long time series and its response to climate factors and human activities were analyzed in the Anhui province. The results indicated that from 2001 to 2016, total NPP had a fluctuated and decreased trend with the variation range between 30.52 and 38.07 TgC in Anhui province. The multi-year average of total NPP was about 34.62 TgC. The highest value was in 2008 and the lowest value was in 2011. Among them, amount of forestland NPP was the most. The spatial distribution of NPP shows that the high value area was mainly distributed in southern Anhui mountain areas and western Anhui Dabie mountain areas; the lower value was distributed in the middle in the study area. The area of which the NPP showed a slight decrease and essentially unchanged accounted for 59.35% and 31.82%, respectively. In general, the correlation between vegetation NPP and temperature was greater than that between precipitation. The vegetations NPP of eight land use types were all positively correlated with temperature. However, the other seven types of land use were negatively correlated with precipitation except cultivated land. In the past 16 years, the decrease of cultivated land areas and the increase of urban and construction land areas contributed a lot to the decrease of vegetation NPP in Anhui province. The NPP changes of different land use types were closely related to climatic factors, land cover area, and vegetation types.

Transformation of copper fractions in rhizosphere soil of two dominant plants in a deserted land of copper tailings.

This paper studied the transformation of copper fractions in rhizosphere and non-rhizosphere soils of Festuca arundinacea and Trifolium repens in deserted land of copper tailings. The results showed that the proportion of organic-bound or exchangeable contents to the total contents of copper in rhizosphere increased by 9.81 and 10.42%, while the contents of carbonate-bound or Fe-Mn oxides-bound decreased by 2.96 and 1.82%, respectively. The growth and absorption of F. arundinacea and T. repens accelerated the transformation of copper fractions in rhizosphere soils. The effect of root system of T. repens was prominently greater than that of F. arundinacea.