Multi-MSIProcessor: Data Visualizing and Analysis Software for Spatial Metabolomics Research.

Mass spectrometry imaging (MSI) has emerged as a revolutionary analytical strategy in biomedical research for molecular visualization. By linking the characterization of functional metabolites with tissue architecture, it is now possible to reveal unknown biological functions of tissues. However, due to the complexity and high dimensionality of MSI data, mining bioinformatics-related peaks from batch MSI data sets and achieving complete spatially resolved metabolomics analysis remain a great challenge. Here, we propose novel MSI data processing software, Multi-MSIProcessor (MMP), which integrates the data read-in, MSI visualization, processed data preservation, and biomarker discovery functions. The MMP focuses on the AFADESI-MSI data platform but also supports mzXML and imzmL data input formats for compatibility with data generated by other MSI platforms such as MALDI/SIMS-MSI. MMP enables deep mining of batch MSI data and has flexible adaptability with the source code opened that welcomes new functions and personalized analysis strategies. Using multiple clinical biosamples with complex heterogeneity, we demonstrated that MMP can rapidly establish complete MSI analysis workflows, assess batch sample data quality, screen and annotate differential MS peaks, and obtain abnormal metabolic pathways. MMP provides a novel platform for spatial metabolomics analysis of multiple samples that could meet the diverse analysis requirements of scholars.

Effects of Mother Voice on Physiological Response and Motor Nerve Development of Newborns with Maternal Separation Under the New Epidemic Situation.

Objective: To investigate an alternative approach to family participatory nursing in neonatal intensive care units (NICUs) during the COVID-19 pandemic, focusing on auditory interventions to mitigate the effects of maternal separation (MS) on neonatal neurological development. Methods: This study was a randomized, double-blind, prospective trial involving 100 newborns younger than 6 months old, born between January 2022 and October 2022, who experienced MS for more than 2 weeks. Newborns were randomly allocated into control and study groups using a computer-generated list to ensure unbiased selection. Inclusion criteria were gestational age ≥37 weeks and admission to NICU due to various medical conditions; exclusion criteria included severe hearing impairment and congenital neurological disorders. The intervention group received maternal voice exposure at 40-50 dB for eight 30-minute sessions daily, while the control group was exposed to children's songs at the same volume and duration. Key metrics such as oxygen saturation, heart rate, Neonatal Infant Pain Scale (NIPS) scores, and Neonatal Behavioral Neurological Assessment (NBNA) scores were measured before and after the intervention period, which lasted one week. Results: Post-intervention, the NIPS scores in the intervention group were significantly lower (3.45±0.99) compared to the control group (5.36±0.49, P < .01), indicating reduced pain sensitivity. Additionally, NBNA scores were higher in the intervention group (39.90±1.56) than in the control group (35.86±1.05, P < .01), suggesting enhanced neurological development. No significant difference in pre-intervention blood oxygen saturation levels was observed between the groups. However, the intervention group showed less reduction in oxygen saturation during and post-blood collection, with significantly higher levels at 2, 4, and 6 minutes post-procedure (P < .01). The findings underscore the significance of maternal voice as a non-pharmacological intervention to alleviate pain and foster neurological development in neonates facing MS, especially in situations where traditional family participatory nursing is hindered by the COVID-19 pandemic. Integrating maternal voice stimulation into neonatal care strategies offers a viable method to improve outcomes for newborns undergoing MS. Conclusion: Maternal voice intervention presents a promising strategy to diminish pain sensitivity and bolster neurological development in neonates separated from their mothers, particularly when family participatory nursing practices are constrained by pandemic-related restrictions. These findings advocate for the broader implementation of maternal voice stimulation in NICU settings.