RESUMO
Brown apical necrosis (BAN) of walnut (Juglans regia L.) causes premature fruit drop and yield losses and has been reported to be an important walnut production problem in Spain, Italy, France, and Turkey (1,2). A number of organisms have been associated with BAN on walnut: Xanthomonas arboricola pv. juglandis, Fusarium spp., and Alternaria spp. (3). Since the spring of 2007, BAN was observed in 50 to 60% of the trees in walnut orchards in Taian City and Laiwu City, Shandong Province, China. Surface-disinfested tissue from premature walnut fruits was placed onto potato dextrose agar. Alternaria spp., X. arboricola pv. juglandis, and Pantoea agglomerans (formerly Enterobacter agglomerans) were isolated 76, 35, and 45% of the time, respectively. The P. agglomerans cultures formed a yellow lawn and were rod shaped with the body length of 1.5 to 3.0 µm, width of 0.5 to 1.0 µm, and four to six flagella. In biochemical tests, these bacteria were gram negative, lactose positive, and indole negative. Genomic DNA was extracted from one HXJ isolate and the 16S rRNA gene sequence (GenBank Accession No. HM016799) was obtained using universal primers 27F and 1492R. HM016799 had 99% sequence identity with P. agglomerans accessions in GenBank (GU477762, GQ494018, FJ756355, and AB004757). To confirm pathogenicity, HXJ isolate (108 CFU·ml-1) was inoculated at the bottom of the stigma within 5 days after florescence (DAF) and in premature fruit wounded with a needle within 30 DAF in 2008 to 2010. Stigmas injected with only sterile water served as controls. The bacteria were inoculated into three replicate 9-year-old plants of the walnut cv. Xiangling. Forty nuts on each plant were inoculated. The plants were grown in Shandong Province, China (36°09'59â³N, 117°13'30â³E). Ten days after inoculation, typical internal BAN symptoms were observed on all treated nuts and the controls were still healthy. In the inoculated stigmas, necrosis of stigma and style spread to internal tissues and reached the kernel. In treated premature fruit, internal tissues became necrotic and blackish and eventually led to nut drop. The same bacterium was reisolated from the inoculated tissue. On the basis of morphological, physiological, and biochemical characteristics and sequencing of the 16S rRNA gene, the bacterium was identified as P. agglomerans. To our knowledge, this is the first report of P. agglomerans causing internal type BAN of walnut in China or worldwide. References: (1) A. Belisario et al. Plant Dis. 6:599, 2002. (2) G. Bouvet. Acta Hortic. 705:447, 2005. (3) C. Moragrega and H. Özaktan. J. Plant Pathol. 92:S1.67, 2010.
RESUMO
BACKGROUND: Renal artery fibromuscular dysplasia (RAFMD) is a non-atherosclerotic cause of renal artery stenosis often affecting the young. Percutaneous transluminal renal angioplasty (PTRA) is the treatment of choice but there are few studies of the outcome of the procedure. METHODS: This retrospective analysis included 64 patients (56.2 % female; mean age at diagnosis, 28.0 years) with RAFMD who underwent PTRA between November 2003 and August 2015. Technical and clinical success rates and restenosis rates were evaluated. RESULTS: Seventy-six procedures were performed on 64 RAFMD patients. Technical success was 96.9 %, as defined by <30 % residual stenosis, with stent placement required in 11 patients (17.2 %). In the short term (1 month), the majority (79.7 %) had an immediate clinical benefit, with cure of hypertension in 35.9 %, and improvement in hypertension and a lower requirement for antihypertensive medications in 43.8 %. In the long term (mean, 47.5 months; range, 5-141 months), the survival rate was 96.9 %, freedom from restenosis was 84.4 %, and 76.6 % of patients showed a sustained clinical benefit (cure rate 40.6 %, improvement rate 35.9 %). Eight patients were treated with a second procedure and two had a third procedure, with half of these patients showing an improvement in hypertension. CONCLUSION: PTRA for symptomatic RAFMD is safe and clinically successful. More than half of patients experience an immediate clinical benefit with sustained long-term effects. For patients with restenosis, there was a good response to a second PTRA.
Assuntos
Angioplastia com Balão , Displasia Fibromuscular/terapia , Hipertensão Renovascular/terapia , Obstrução da Artéria Renal/terapia , Artéria Renal , Adolescente , Adulto , Angiografia Digital , Angioplastia com Balão/efeitos adversos , Angioplastia com Balão/instrumentação , Angioplastia com Balão/mortalidade , Intervalo Livre de Doença , Feminino , Displasia Fibromuscular/complicações , Displasia Fibromuscular/diagnóstico por imagem , Displasia Fibromuscular/mortalidade , Humanos , Hipertensão Renovascular/diagnóstico , Hipertensão Renovascular/etiologia , Hipertensão Renovascular/mortalidade , Masculino , Pessoa de Meia-Idade , Recidiva , Artéria Renal/diagnóstico por imagem , Obstrução da Artéria Renal/diagnóstico por imagem , Obstrução da Artéria Renal/etiologia , Obstrução da Artéria Renal/mortalidade , Retratamento , Estudos Retrospectivos , Fatores de Risco , Stents , Fatores de Tempo , Resultado do Tratamento , Adulto JovemRESUMO
Based on clinical analysis of 87 cases with congenital fusion of cervical vertebrae, the clinical significance of these lesions was discussed in detail. It was found that the C 2-3 and then the C 3-4 were the sites of frequent involvement. Fusion of 2 to 3 segments was common and showed no clinical signs of abnormality, nor associated with any other malformations. While fusion of multiple segments, though rare, was often associated with other anomalies such as short neck, lowered posterior hair line, webbed neck and malformation of internal organs and other bones. Single lesion does not give rise to symptoms until late when degenerative changes have taken place in the unfused vertebrae in most cases, or following trauma in occasional patients, usually of neurologic upsets. However, fusion at several especially levels, fusion of C 2-3 with occipitalization of atlas predisposes the nerve to damage. In short, remedy is only necessary for cases with symptoms: conservative treatment for those with symptoms caused by radiculopathy, and operation for those with symptoms of myelopathy.
Assuntos
Vértebras Cervicais/anormalidades , Sinostose , Adolescente , Adulto , Vértebras Cervicais/cirurgia , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , Sinostose/diagnóstico por imagem , Sinostose/cirurgiaAssuntos
Anquilose/cirurgia , Doenças do Sistema Nervoso Autônomo/cirurgia , Vértebras Cervicais , Adulto , Idoso , Anquilose/complicações , Anquilose/diagnóstico , Doenças do Sistema Nervoso Autônomo/complicações , Doenças do Sistema Nervoso Autônomo/diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sistema Nervoso SimpáticoRESUMO
A plasmid, pGB112, has recently been developed to transfer DNA from Escherichia coli to Streptomyces spp via conjugation. This technique made use of (A) E. coli replicon, (B) ampicillin (amp) resistance gene for selection in E. coli and thiostrepton (tsr) resistance gene for selection in Streptomyces, (C) a fragment of SCP2* replicon, (D) a 2.6 kb fragment of tra-cassette which consists of pIJ101 transfer gene (tra) and two ermE promoters, (E) a 0.8 kb fragment of oriT of (IncP) RK2. The results showed that this plasmid was able to transfer plasmid DNA from E. coli to Streptomyces coelicolor via conjugation, and that it could also transfer DNA between Streptomyces strains. Since this plasmid has both pBR322 and SCP2* replicons, it may provide a novel and useful method for genetic operation in E. coli and Streptomyces.
Assuntos
Conjugação Genética , Plasmídeos/genética , Streptomyces/genética , Transformação Bacteriana , DNA Bacteriano/genética , Escherichia coli/genética , Reação em Cadeia da Polimerase , Mapeamento por RestriçãoRESUMO
The purpose of this study is to find out the better conditions for filling blood vessels of the body with latex. We test it on 500 bodies. Our data show that the temperature for storing the latex, filling positions, the wash of blood vessels prior to filling and filling pressure are all important in this process. A good filling increases elasticity of vessels, makes vessels stronger and facilitates dissection of vessel. It is of importance in the study of cardiovascular system.
Assuntos
Vasos Sanguíneos , Embalsamamento/métodos , Látex , Vasos Sanguíneos/anatomia & histologia , Humanos , PerfusãoRESUMO
The coronary arteries of 50 goats were studied by means of angiography and dissection. The results were as follows: The right coronary artery (RCA) and left coronary artery (LCA) arose from the right and left aortic sinuses respectively. At the beginning, the average external diameter of RCA (2.98 +/- 1.02 mm) was smaller than that of LCA (3.42 +/- 0.74 mm). An independent inter ventricular septal artery (in 94%) supplied the upper two-thirds of the inter ventricular septum. The arterial distributive types were divided into three: The LCA was dominant in 96%, symmetrical in 4%, RCA dominant in 0%. Sixty two percent of the S-A node arteries arose from the RCA, 28% from LCA, 10% from both RCA and LCA. All the A-V node arteries originated from the LCA.
Assuntos
Angiografia Coronária , Animais , Biometria , Vasos Coronários/anatomia & histologia , Sistema de Condução Cardíaco/anatomia & histologia , OvinosRESUMO
The therapeutic effect of arsenic trioxide (As2O3) in the treatment of acute promyelocytic leukemia (APL) was evaluated among 15 APL patients at relapse after all-trans retinoic acid (ATRA) induced and chemotherapy maintained complete remission (CR). As2O3 was administered intravenously at the dose of 10 mg/d. Clinical CR was achieved in nine of 10 (90%) patients treated with As2O3 alone and in the remaining five patients treated by the combination of As2O3 and low-dose chemotherapeutic drugs or ATRA. During the treatment with As2O3, there was no bone marrow depression and only limited side effects were encountered. Pharmacokinetic studies, which were performed in eight patients, showed that after a peak level of 5.54 micromol/L to 7.30 micromol/L, plasma arsenic was rapidly eliminated, and the continuous administration of As2O3 did not alter its pharmacokinetic behaviors. In addition, increased amounts of arsenic appeared in the urine, with a daily excretion accounting for approximately 1% to 8% of the total daily dose administered. Arsenic contents in hair and nail were increased, and the peak content of arsenic could reach 2.5 to 2.7 microg/g tissue at CR. On the other hand, a decline of the arsenic content in hair and nail was observed after withdrawal of the drug. We conclude that As2O3 treatment is an effective and relatively safe drug in APL patients refractory to ATRA and conventional chemotherapy.