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1.
AIDS Care ; 34(8): 1041-1047, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-34156890

RESUMO

Medication adherence to antiretroviral therapy (ART) among elderly people living with HIV (PLWH) is of serious concern. Our study aimed to understand the medication adherence of elderly PLWH under ART based on the health belief model (HBM). A baseline survey with a total of 529 elderly PLWH was conducted in Sichuan. Logistic and linear regression analysis, mediation analysis, and path analysis based on prior evidence were used. Only self-efficacy showed direct associations with medication adherence in the last four days (ORm = 1.37, 95%CI: 1.11, 1.70) and the last month (ORm = 1.39, 95%CI: 1.18, 1.63) in the multivariate analysis. Self-efficacy mediated the relations between perceived benefits, perceived barriers, cues to action and medication adherence. Inner relations existed within the HBM. In addition to the direct effects, perceived benefits (ß = 0.149, p = 0.031; ß = 0.093, p = 0.005), perceived barriers (ß = -0.070, p = 0.008; ß = -0.062, p = 0.012), and cues to action (ß = 0.184, p = 0.013; ß = 0.135, p = 0.014) showed indirect effects on medication adherence in the last four days and the last month, respectively. HBM may be effective in predicting medication adherence of elderly PLWH, and self-efficacy may be a crucial predictor and mediator. Efforts should be focused on how to enhance elderly PLWH's self-efficacy without neglect of other medication beliefs.


Assuntos
Infecções por HIV , Autoeficácia , Idoso , Infecções por HIV/tratamento farmacológico , Infecções por HIV/psicologia , Modelo de Crenças de Saúde , Humanos , Adesão à Medicação/psicologia , Inquéritos e Questionários
2.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(2): 225-230, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32220192

RESUMO

OBJECTIVE: To analyze the relationship between community social capital and quality of life among the middle-aged and elderly rural-to-urban residents, and to provide the policy reference for improving the health status. METHODS: A multi-stage random sampling method was used to select the research objects. Univariate analysis and logistic regression model were used to explore the effect of social capital on quality of life among the middle-aged and elderly rural-to-urban urbanized residents. RESULTS: The scores of self-rated physical health and mental health in the rural-to-urban residents were lower than those of urban residents ( P<0.05). The total score of community social capital, community participation and community cohesion in the rural-to-urban residents were lower than those of urban residents ( P<0.05). The result of multivariate analysis showed that community attachment and community cohesion were the protective factors of physical health ( P<0.05), and community cohesion was the protective factor of mental health ( P<0.05). CONCLUSION: There is a correlation between community belonging, community cohesion and quality of life among the elderly rural-to-urban residents. Attention should be paid to the promotion of community social capital so as to improve the health status of middle-aged and elderly rural-to-urban residents.


Assuntos
Qualidade de Vida , População Rural , Capital Social , População Urbana , Idoso , Nível de Saúde , Humanos , Pessoa de Meia-Idade
3.
Biomed Environ Sci ; 30(10): 701-707, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29122090

RESUMO

OBJECTIVE: To analyze the prevalence and years lived with disability (YLD) from dental caries among children and adolescents and the time trends over the past two decades in Sichuan province, the largest province in west China. METHODS: Based on the Global Burden of Disease Study 2015 (GBD2015), which systematically assessed the epidemiological characteristics of major diseases and their transitions by country and region from 1990 to 2015, we extracted the estimated results for China. We then used the Bayesian meta-regression method to estimate the sex- and age-specific prevalences and YLDs from dental caries among children and adolescents under 15 years old in Sichuan province and compared them with global and national indicators for the same period. RESULTS: In 2015, there were almost 6 million cases of dental caries in children and adolescents (aged < 15 years) in Sichuan province, accounting for 6% of the total cases in China. For children under 5 years, the prevalence of deciduous caries was 55.9%, and the YLDs value was 10.8 per 100,000, while it was 24.3% and 5.1 per 100,000 respectively among 5- to 14-year-olds; for those aged 5 to 14 years, the prevalence of permanent caries was 21.5%, and the YLDs value was 11.5 per 100,000. From 1990 to 2015, the prevalence of dental caries for children under 5 years increased substantially, by 16.2%, and the YLDs increased by 8.7%. Among those aged 5 to 14 years, the prevalence increased and the YLDs decreased. CONCLUSION: Dental caries remains a huge health burden in Western China. In contrast to the global and national data, the trend has increased rapidly over the past 25 years in this region. This work provides suggestions for the prevention and control for oral health in China with the policy of two-child.


Assuntos
Cárie Dentária/epidemiologia , Cárie Dentária/patologia , Adolescente , Criança , Pré-Escolar , China/epidemiologia , Pessoas com Deficiência , Feminino , Humanos , Masculino , Prevalência
4.
Tumour Biol ; 35(8): 7775-80, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24816919

RESUMO

MicroRNAs have been demonstrated to have a role in susceptibility and prognosis of various types of human cancer. We investigated the association between polymorphisms in miR-146aG>C, miR-196a2C>T, and miR-499A>G and hepatocellular carcinoma (HCC) risk and interaction with HCC and hepatitis B virus (HBV) infection. Two hundred sixty-six cases with HCC and 281 health controls were enrolled in the present study. Genotyping of the miR-146aG>C, miR-196a2C>T, and miR-499A>G genotypes was conducted by duplex polymerase chain reaction with the confronting two-pair primer (PCR-RFLP). Subjects with miR-146a GG and G allele had an increased risk of HCC compared with the homozygote CC genotype. Similarly, HCC patients carrying microRNA (miRNA)-196a2 computed tomography, TT, and T allele significantly decreased the risk of HCC relative to the CC genotype. Stratified analysis indicated that miR-196a2C>T polymorphism was associated with reduced risk of HBV-related HCC, but not in hepatitis C virus- and nonviral-related HCC cases. In conclusion, miR-146aG>C and miR-196a2C>T polymorphism are associated with risk of HCC patients in China, especially in patients with HBV infection. SNPs in miRNA sequences can be used as a diagnostic biomarker for HCC.


Assuntos
Carcinoma Hepatocelular/genética , Predisposição Genética para Doença , Neoplasias Hepáticas/genética , MicroRNAs/fisiologia , Polimorfismo Genético , Adulto , Idoso , Povo Asiático/genética , Carcinoma Hepatocelular/etiologia , China , Feminino , Genótipo , Humanos , Neoplasias Hepáticas/etiologia , Masculino , Pessoa de Meia-Idade , Risco
5.
Zhong Yao Cai ; 37(1): 74-6, 2014 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-25090710

RESUMO

OBJECTIVE: To study the chemical constituents of Pattra Medicine Euodia lepta in Xishuangbanna of Yunnan Province. METHODS: The chemical constituents were isolated and purified by chromatographic techniques, and identified by NMR, MS and other spectral methods. RESULTS: In 60% ethanol extract from the stems,and 95% ethanol extract from the leaves, six compounds and two compounds were isolated and identified as pachypodol( 1) ,3-(3-methyl-but-2-enyl )umbelliferone(2),7-demethylsuberosin (3),beta-sitosterol (4),3,7-dimethoxy kaempferol(5), euolitrine(6), sesamin(7) and p-O-geranyl coumaric acid(8), respectively. CONCLUSION: Compound 7 is obtained from Euodia genus for the first time,and compound 8 is obtained from domestic Euodia lepta for the first time.


Assuntos
Ácidos Cumáricos/química , Dioxóis/química , Evodia/química , Furocumarinas/química , Lignanas/química , China , Ácidos Cumáricos/isolamento & purificação , Dioxóis/isolamento & purificação , Furocumarinas/isolamento & purificação , Lignanas/isolamento & purificação , Folhas de Planta/química , Caules de Planta/química , Sitosteroides/química , Sitosteroides/isolamento & purificação
6.
Sci Rep ; 14(1): 14639, 2024 06 25.
Artigo em Inglês | MEDLINE | ID: mdl-38918463

RESUMO

This study aimed to develop a deep learning model to predict the risk stratification of all-cause death for older people with disability, providing guidance for long-term care plans. Based on the government-led long-term care insurance program in a pilot city of China from 2017 and followed up to 2021, the study included 42,353 disabled adults aged over 65, with 25,071 assigned to the training set and 17,282 to the validation set. The administrative data (including baseline characteristics, underlying medical conditions, and all-cause mortality) were collected to develop a deep learning model by least absolute shrinkage and selection operator. After a median follow-up time of 14 months, 17,565 (41.5%) deaths were recorded. Thirty predictors were identified and included in the final models for disability-related deaths. Physical disability (mobility, incontinence, feeding), adverse events (pressure ulcers and falls from bed), and cancer were related to poor prognosis. A total of 10,127, 25,140 and 7086 individuals were classified into low-, medium-, and high-risk groups, with actual risk probabilities of death of 9.5%, 45.8%, and 85.5%, respectively. This deep learning model could facilitate the prevention of risk factors and provide guidance for long-term care model planning based on risk stratification.


Assuntos
Aprendizado Profundo , Assistência de Longa Duração , Humanos , Feminino , Masculino , Idoso , China/epidemiologia , Estudos Prospectivos , Idoso de 80 Anos ou mais , Causas de Morte , Pessoas com Deficiência/estatística & dados numéricos , Medição de Risco , Mortalidade/tendências , Fatores de Risco , Prognóstico
7.
Int J Cardiol ; 371: 480-485, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36115439

RESUMO

BACKGROUND: The dynamic changes and apical aneurysm formation in apical hypertrophic cardiomyopathy (HCM) have not been specifically described. This study aimed to describe these changes to better understand the progression of apical HCM. METHODS AND RESULTS: Seventy-two patients with apical HCM who underwent at least two cardiac magnetic resonance (CMR) examinations were retrospectively included in this study. The mean interval between the first and last CMR examinations was 50.1 ± 26.8 months (ranging from 4 to 118 months). Compared with the initial values, the left atrial diameter, maximum left ventricular wall thickness and late gadolinium enhancement extent significantly increased (all P < 0.05), while the left ventricular ejection fraction significantly decreased (P < 0.05), at the latest CMR examination. More importantly, the dynamic process of apical aneurysm formation in apical HCM was observed in a subset of patients, which may follow these four stages: starting with systolic apical cavity obliteration, then broadening of the apical slit in systole, further developing into an apical outpouching, and finally forming an apical aneurysm. Eleven patients experienced adverse cardiovascular events, including new-onset or progressive atrial fibrillation (n = 7), hospitalization with heart failure (n = 3) and implantable cardioverter defibrillator intervention (n = 1), at the time of the latest CMR examination. CONCLUSIONS: In the progression of apical HCM, cardiac structure and function will change accordingly. Apical aneurysm formation in apical HCM is a chronic and continuous dynamic process that may follow a 4-step pathway of disease progression.


Assuntos
Aneurisma , Miocardiopatia Hipertrófica Apical , Cardiomiopatia Hipertrófica , Humanos , Projetos Piloto , Gadolínio , Meios de Contraste , Função Ventricular Esquerda , Volume Sistólico , Estudos Retrospectivos , Espectroscopia de Ressonância Magnética
8.
Int Arch Occup Environ Health ; 85(1): 35-43, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21559941

RESUMO

OBJECTIVE: To determine whether personal strain and coping resources act as either mediator or moderator or both in the relationship between work stressor and quality of life among Chinese nurses. METHODS: A total of 1,012 nurses were selected from eight hospitals located in two provinces in China. Quality of life was measured with the Chinese version of the Short Form-36 Health Survey; work stressor, personal strain, and coping resources were evaluated using the Occupation Stress Inventory-Revised Edition. The hierarchical multiple regression procedure and Baron and Kenny's model of mediation were applied to test for moderation and mediation, respectively. A structural equation model was fit to assess the interrelationships among these variables. RESULTS: Work stressor was closely associated with quality of life, which was mediated and moderated by personal strain and coping resources. Personal strain also acted both as moderator and mediator in the relationship between coping resources and quality of life. The relationships were verified in the structural equation model. The greatest absolute value of the standardized total effects was seen in personal strain (0.817), followed by work stressor (0.634) and coping resources (0.488). CONCLUSIONS: Personal strain and coping resources have both mediating and moderating effects on the relationship between work stress and quality of life in a sample of Chinese nurses. An effective intervention strategy is needed to reduce work stress and ensure better quality of life.


Assuntos
Adaptação Psicológica , Esgotamento Profissional/psicologia , Enfermeiras e Enfermeiros/psicologia , Doenças Profissionais/psicologia , Estresse Psicológico/psicologia , Local de Trabalho/psicologia , Adolescente , Adulto , Esgotamento Profissional/etiologia , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/etiologia , Doenças Profissionais/prevenção & controle , Qualidade de Vida , Adulto Jovem
9.
Zhong Yao Cai ; 33(10): 1551-3, 2010 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-21355190

RESUMO

OBJECTIVE: To research the changes of chemical constituent in Inula nervosa during the processing. METHOD: Inula nervosa processed with different conditions was determined by HPLC,with acetonitrile--water as a mobile phase using gradient elution, and the detective wavelength was 280 nm. The emerging compounds 1 was extracted by 50% ethanol, isolated and purified using chromatography, and identified according to the physicochemical properties and spectral analysis. RESULTS: The HPLC chromatogram of lnula nervosa changes before and after processing. A monomer compounds was isolated from processed Inula nervosa. It was Identified as 5-hydroxymethylfurfural. CONCLUSION: The chemical constituent of Inula nervosa changes during the processing. It is the first time that compound 1 were isolated from Inula.


Assuntos
Medicamentos de Ervas Chinesas/química , Furaldeído/análogos & derivados , Inula/química , Plantas Medicinais/química , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/isolamento & purificação , Furaldeído/análise , Furaldeído/isolamento & purificação , Óleos Voláteis/análise , Óleos Voláteis/isolamento & purificação , Raízes de Plantas/química , Solventes/química , Tecnologia Farmacêutica/métodos , Temperatura , Fatores de Tempo
10.
Plant Reprod ; 33(1): 35-40, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31997012

RESUMO

KEY MESSAGE: Sperm cells can be isolated from the mature pollen grains of medicinal wild rice (Oryza officinalis) using an osmotic shock method, and the viable egg cells can be isolated by enzymatic digestion and mechanical dissection steps. Favorable alleles for rice breeding have been identified in natural cultivars and wild rice by association analysis of known functional genes with target trait performance. Transferring these genes from wild rice into cultivated rice varieties is one of the important objectives for rice breeders. The isolation of the sperm and egg cells of wild and cultivated rice is a prerequisite for the in vitro hybridization of distantly related cultivated rice and wild rice lines. Here, we provide a technical approach for isolating the sperm and egg cells of wild rice (Oryza officinalis). In this method, sperm cells were isolated from the mature pollen grains of medicinal wild rice (O. officinalis) according to an osmotic shock method. Additionally, viable O. officinalis egg cells were isolated following enzymatic digestion and mechanical dissection steps. Specifically, ovules were digested in an enzymatic solution containing pectinase and cellulase for 30 min, after which the ovule was cut into two halves. Three egg apparatus cells were released by gently applying pressure to the micropylar end. Generally, six or seven egg cells could be isolated from 20 ovules in 60 min. The same method was used to isolate zygotes from flowers at 24 h after pollination. This technology solved the difficulty of isolating sperm and egg cells in O. officinalis and allowed the isolated sperm and egg cells to be combined by in vitro hybridization of distantly related wild and cultivated rice lines.


Assuntos
Agricultura , Células Germinativas Vegetais , Oryza , Agricultura/métodos , Produção Agrícola , Flores/fisiologia , Células Germinativas Vegetais/fisiologia , Óvulo Vegetal , Pólen
11.
Plant Reprod ; 33(1): 41, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32055991

RESUMO

The funding section of the original publication gave a wrong funding number.

12.
Artigo em Chinês | MEDLINE | ID: mdl-20067003

RESUMO

Freshwater crabs and snails were collected from Ninghai County in Zhejiang Province, and examined respectively for Paragonimus metacercariae and cercariae. Among 97 freshwater crabs found, the prevalence was 11.3% (11/97) with a mean intensity of 1 metacercariae per crab. It was 10.2% (5/49) and 20.2% (4/20) in the groups weighted 5-15 g and 15-25 g respectively, with an average intensity of 1, and no metacercariae were found in weight group of 25-35 g. Two positive crabs were found from 20 crabs with a low weight (< 5 g). Male to female crabs ratio was 2.5:1, and there was no significant difference in prevalence between males [12.7%(7/55)] and females [9.1% (2/22)]. No cercariae or metacercariae were found in 200 snails (Semisulcospira libertino).


Assuntos
Braquiúros/parasitologia , Paragonimíase/epidemiologia , Paragonimus , Caramujos/parasitologia , Animais , China/epidemiologia , Feminino , Água Doce , Interações Hospedeiro-Parasita , Masculino , Paragonimíase/parasitologia
13.
World J Gastroenterol ; 13(43): 5760-4, 2007 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-17963305

RESUMO

AIM: To evaluate the impact of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) polymorphisms on esophageal cancer risk. METHODS: One hundred and ninety-one esophageal cancer patients and 198 healthy controls from Yanting County were enrolled in this study. ADH2 and ALDH2 genotypes were examined by polymerase-chain-reaction with the confronting-two-pair-primer (PCR-CTPP) method. Unconditional logistic regression was used to calculate the odds ratios (OR) and 95% confidence interval (95% CI). RESULTS: Both ADH2 1 allele and ALDH2 1/ 2 allele showed an increased risk of developing esophageal cancer. The adjusted OR (95% CI) for ADH2 1 allele compared with ADH2 2/ 2 was 1.65 (95% CI = 1.02-2.68) and 1.67 (95% CI = 1.02-2.72) for ALDH2 1/ 2 compared with ALDH2 1/ 1. A significant interaction between ALDH2 and drinking was detected regarding esophageal cancer risk, the OR was 1.83 (95% CI = 1.13-2.95). Furthermore, when compared with ADH2 2/ 2 and ALDH2 1/ 1 carriers, ADH2 1 and ALDH2 2 carriers showed an elevated risk of developing esophageal cancer among non-alcohol drinkers (OR = 2.46, 95% CI = 0.98-6.14), and a significantly elevated risk of developing esophageal cancer among alcohol drinkers among alcohol drinkers (OR = 9.86, 95% CI = 3.10-31.38). CONCLUSION: ADH2 and ALDH2 genotypes are associated with esophageal cancer risk. ADH2 1 allele and ALDH2 2 allele carriers have a much higher risk of developing esophageal cancer, especially among alcohol drinkers.


Assuntos
Álcool Desidrogenase/genética , Aldeído Desidrogenase/genética , Neoplasias Esofágicas/genética , Polimorfismo Genético/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Consumo de Bebidas Alcoólicas/efeitos adversos , Aldeído-Desidrogenase Mitocondrial , Alelos , Povo Asiático/genética , Estudos de Casos e Controles , China , Neoplasias Esofágicas/etnologia , Feminino , Genótipo , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Fatores de Risco
14.
Yi Chuan ; 24(5): 551-4, 2002 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-16135448

RESUMO

The development of modern molecular biological techniques makes it possible to study minimum DNA from ancient materials. During past decade,a lot of significant achievements on ancient DNA research have been made in many fields especially in molecular evolutionary biology. The nature of degradation and contamination of ancient DNA from ancient biological materials pose a dominating problem in ancient DNA research. Therefore,the experiments should be modified based on the modern molecular techniques and more factors should be considered when the results are analyzed. In this paper,authors review the general experimental protocols on sampling,extraction and amplification as well as authenticity of ancient DNA.

15.
Eur J Cancer Prev ; 23(1): 35-42, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24080970

RESUMO

With an estimate of 380 000 new cases each year, gastric cancer (GC) is one of the most frequently occurring cancers in China. Genes encoding proinflammatory and anti-inflammatory cytokines are good candidates for the study of susceptibility to GC. We tested the hypothesis that the polymorphisms of interleukin 1B (IL-1B) and IL-1RN contribute toward host susceptibility to GC. In a matched case-control design, we enrolled 308 pairs of GC and control participants between October 2010 and August 2011. We sequenced IL-1B +3954 C/T, IL-1RN -9876 G/A, -9739 A/G, and IL-1RN -9091 A/C using MALDI-TOF MS and collected demographic data as well as lifestyle factors using a questionnaire. GC patients reported statistically significantly greater proportions with family history of cancer (29.9 vs. 10.7%, P<0.01) and alcohol drinking (54.5 vs. 43.2%, P<0.01) than the controls. The proportion of irregular eaters was statistically higher among the patients than among the controls (66.7 vs. 24.4%, P<0.01). The IL-1B +3954 CT or the TT variant genotype was statistically significantly associated with a risk of GC [adjusted odds ratio (OR), 2.94; 95% confidence interval (CI), 1.06-8.15], whereas variants of IL-1RN -9876 G/A, IL-1RN -9739 A/G, and IL-1RN -9091 A/C were not associated (adjusted OR, 1.29, 95% CI, 0.77-2.16; adjusted OR, 1.25, 95% CI, 0.75-2.07; adjusted OR, 1.09, 95% CI, 0.71-1.67, respectively). Haplotypes established from the three polymorphisms of IL-1RN were not associated with a risk of GC. The IL-1B +3954 C/T polymorphism is associated with a risk of GC in our study. Lifestyle and environmental factors such as drinking, eating irregularly, and family history of cancer increase the risk.


Assuntos
Predisposição Genética para Doença , Interleucina-1beta/genética , Polimorfismo de Nucleotídeo Único , Neoplasias Gástricas/genética , Estudos de Casos e Controles , China/epidemiologia , Feminino , Seguimentos , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Fatores de Risco , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Neoplasias Gástricas/epidemiologia
16.
Asian Pac J Cancer Prev ; 15(7): 2951-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24815430

RESUMO

BACKGROUND: Gastric carcinogenesis is a complicated process that involves environmental and genetic factors like interleukin-4 (IL-4) and IL-8. Single nucleotide polymorphisms in their genes are associated with changed levels of gene expression. Here, we investigated the association between IL4-590 C>T and IL8-251T>A and gastric cancer (GC) risk in Sichuan of Southwestern China. MATERIALS AND METHODS: We surveyed the research subjects using a self-designed questionnaire with questions on demographic factors and putative risk factors. Approximately 2-5ml of whole blood was collected after field survey to analyze IL4-590 C>T and IL8-251T>A genotypes using MALDI-TOF MS. RESULTS: Our study recruited 308 pairs of GC patients and controls, including 224 (72.7%) men and 84 (27.3%) women in each group. There were 99 cardia and 176 noncardia GC patients in the case group. The case and control groups had an average age of 57.7±10.6 (mean±SD) and 57.6±11.1 years. GC patients reported a significantly greater proportion of family history of cancer (29.9% vs 10.7%, p<0.01) and drinking (54.6% vs 43.2%, p<0.01) than did controls. Variant genotypes of IL-4-590 C>T and IL-8-251 T>A were not associated with overall GC risk (adjusted OR, 0.89; 95%CI, 0.61-1.28 for CT or CC vs TT; adjusted OR, 1.14; 95%CI, 0.86-1.79 for TA or AA vs TT). Stratification analysis of two SNPs for risk by subsites only found that variant IL-8-251 TA or AA genotype was associated with increased noncardia GC risk (adjusted OR, 2.58; 95%CI, 1.19-5.57). We did not observe interactions between the IL-8-251 T>A genotype and smoking (adjusted OR, 0.38; 95%CI, 0.08-1.79) or drinking (adjusted OR, 0.36; 95%CI, 0.08-1.65) for risk of noncardia GC. CONCLUSIONS: Our data indicate no association between the two SNPs of IL-4-590 and IL-8-251 with overall GC risk, while the IL-8-251 TA or AA genotype conferred risk of cardia GC. Our findings contribute to the evidence body for risk of SNPs associated with the development of gastric cancer in this region.


Assuntos
Interleucina-4/genética , Interleucina-8/genética , Neoplasias Gástricas/epidemiologia , Neoplasias Gástricas/genética , Cárdia/patologia , Estudos de Casos e Controles , China/epidemiologia , Demografia , Família , Feminino , Frequência do Gene , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Fatores de Risco , Inquéritos e Questionários
17.
Asian Pac J Cancer Prev ; 14(1): 21-5, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23534726

RESUMO

BACKGROUND: Genetic factors and environmental factors play a role in pathogenesis of esophageal squamous cell carcinoma (ESCC). Previous studies regarding the association of folate intake and Methylenetetrahydrofolate reductase C677T polymorphism with ESCC was conflicting. We conducted a meta-analysis to investigate the association of MTHFR C677T and folate intake with esophageal cancer risk. METHODS: MEDLINE, EMBASE and the Chinese Biomedical Database were searched in our study. The quality of studies were evaluated by predefined scale, and The association of polymorphisms of MTHFR C677T and folate intake and ESCC risk was estimated by Odds ratio (ORs) with 95% confidence intervals (CIs). RESULTS: 19 studies (4239 cases and 5575 controls) were included for meta-analysis. A significant association was seen between individuals with MTHFR 677 CT [OR(95%)=1.47(1.32-1.63)] and TT [OR(95%)=1.69(1.49-1.91)] genotypes and ESCC risk (p<0.05). Low intake of folate had significantly higher risk of esophageal cancer among individuals with CT/TT genotype [OR(95%)=1.65(1.1-2.49)], while high intake of folate did not find significant high risk of esophageal cancer among individuals with CT/TT genotype [OR(95%)=1.64 (0.82-3.26)]. CONCLUSIONS: Our meta-analysis indicated the folate intake and MTHFR 677CT/TT are associated with the risk of ESCC, and folate showed a significant interaction with polymorphism of MTHFR C677T.


Assuntos
Carcinoma de Células Escamosas/genética , Neoplasias Esofágicas/genética , Ácido Fólico , Predisposição Genética para Doença , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Estudos de Casos e Controles , Intervalos de Confiança , Dieta , Genótipo , Humanos , Razão de Chances , Polimorfismo de Nucleotídeo Único , Fatores de Risco
18.
Asian Pac J Cancer Prev ; 14(4): 2577-82, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23725178

RESUMO

OBJECTIVES: Interleukin (IL) -10 is a potent cytokine with a dual ability to immunosuppress or immunostimulate. We aimed to explore the association of IL10 promoter polymorphisms with risk of gastric cancer (GC) in a Han population in Southwestern China. METHODS: We enrolled 308 pairs of GC and control subjects from four hospitals and a community between October 2010 and August 2011 in a 1:1 matched case-control design. Demographic information was collected using a designed questionnaire. IL10-592 A>C and IL10-1082 A>G polymorphisms were determined by Sequenom MassARRAY analysis. RESULTS: Patients with GC reported statistically higher proportions of family history of cancer (29.9% versus 10.7%, P<0.01) and alcohol drinking (54.6% versus 43.2%, P<0.01) than did controls. Similar results were observed in comparison between non-cardia GC patients and controls (P<0.01 and P=0.03). Variant genotypes of IL10-592 A>C and IL10-1082 A>G were not associated with overall GC risk (adjusted OR, 0.94, 95% CI, 0.66-1.33; adjusted OR, 1.00, 95% CI, 0.62-1.60). Sub-analysis showed that the IL10-592 AC/CC variant genotype was associated with decreased non-cardia GC risk (adjusted OR, 0.58; 95% CI, 0.36-0.95). No association was found between any of the IL10 haplotypes established from two polymorphisms and risk of non-cardia GC. CONCLUSIONS: In conclusion, our data do not link the two SNPs of IL10-592 and IL10-1082 with overall GC risk. We demonstrate that IL10-592 polymorphism is associated with protective effect against non-cardia GC. Our findings may offer insight into risk associated with the development of GC in this region.


Assuntos
Haplótipos/genética , Interleucina-10/genética , Polimorfismo de Nucleotídeo Único/genética , Regiões Promotoras Genéticas/genética , Neoplasias Gástricas/etiologia , Estudos de Casos e Controles , China/epidemiologia , Feminino , Seguimentos , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Fatores de Risco , Neoplasias Gástricas/patologia , Taxa de Sobrevida
19.
Asian Pac J Cancer Prev ; 13(12): 6187-90, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23464428

RESUMO

We aimed to investigate DNA repair gene expression of response to chemotherapy among gastric patients, and roles in the prognosis of gastric cancer. A total of 209 gastric cancer patients were included in this study between January 2007 and December 2008, all treated with chemotherapy. Polymorphisms were detected by real time PCR with TaqMan probes, and genomic DNA was extracted from peripheral blood samples. The overall response rate was 61.2%. The median progression and overall survivals were 8.5 and 18.7 months, respectively. A significant increased treatment response was found among patients with XPG C/T+T/T or XRCC1 399G/ A+A/A genotypes, with the OR (95% CI) of 2.14 (1.15-4.01) and 1.75 (1.04-3.35) respectively. We found XPG C/T+T/T and XRCC1 399 G/A+A/A were associated with a longer survival among gastric cancer patients when compared with their wide type genotypes, with HRs and 95% CIs of 0.49 (0.27-0.89) and 0.56 (0.29-0.98) respectively. Selecting specific chemotherapy based on pretreatment genotyping may be an innovative strategy for further studies.


Assuntos
Polimorfismo de Nucleotídeo Único , Neoplasias Gástricas , Reparo do DNA , Genótipo , Humanos , Prognóstico , Neoplasias Gástricas/genética
20.
Ying Yong Sheng Tai Xue Bao ; 23(2): 363-8, 2012 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-22586959

RESUMO

To approach the action mechanisms of desulfurization waste on alleviating alkali stress-induced injury of rice, a pot experiment was conducted to study the variations of leaf total calcium content, calcium distribution, plasma membrane Ca(2+)-ATPase activity, and reactive oxygen content of rice seedlings under alkali stress after the application of desulfurization waste. In the control, a few calcium particulates scattered in the cell wall and chloroplasts, while applying desulfurization waste or CaSO4 increased the calcium particulates in the plasma membrane, intercellular space, cell wall, and vacuole significantly. With the increasing application rate of desulfurization waste or CaSO4, the leaf total calcium content increased, Ca(2+)-ATPase activity in plasma membrane and tonoplast presented an increasing trend, plasma membrane relative permeability, MDA content, and O2 production rate decreased, and SOD and POD activities increased. The desulfurization waste could relieve the alkali stress to rice in some extent, and the main reactive compound in the waste could be CaSO4.


Assuntos
Álcalis/farmacologia , ATPases Transportadoras de Cálcio/metabolismo , Cálcio/metabolismo , Oryza/metabolismo , Estresse Fisiológico/fisiologia , Álcalis/química , Sulfato de Cálcio/química , Ecossistema , Oryza/fisiologia , Folhas de Planta/metabolismo , Solo/análise , Superóxido Dismutase/metabolismo
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