Influence of smoothened siRNA on human esophageal cancer cell line EC9706 proliferation and apoptosis.

Apply siRNA technology to take Smoothened (Smo) genetic locus, the possibility of Hhsignaling pathway, and also inhibits esophageal squamous carcinoma cell proliferation and induction of apoptosis. Utilizing SmosiRNA transfected esophageal squamous carcinoma EC9706 cells, adopting RT-PCR and Western blot technology to detect Smo, Gli1 mRNA and protein expression in each group of cells, employing MTT and FCM to detect the influence of SmosiRNA on cell proliferation and apoptosis. Compared with each control group, after transfected SmosiRNA transfected cell for 24h, 48 h and 72 h, SmosiRNA and Gli1 mRNA expression was evenly reduced obviously, Smo and Gli1 protein expression level was also reduced clearly. SmosiRNA could inhabit cell proliferation, and even promote cell apoptosis. Smo gene played a role in regulating and controlling esophageal squamous carcinoma cell proliferation and apoptosis and Smo was likely to be an effective target spot for tumor gene therapy.

Clinicopathologic analysis of micronodular thymoma with lymphoid stroma / 中华病理学杂志

Objective@#To investigate the clinicopathologic features of micronodular thymoma with lymphoid stroma(MNT).@*Methods@#Five cases of MNT diagnosed from January 2007 to December 2016 in Henan Provincial People′s Hospital were collected.Hematoxylin-Eosin staining and immunohistochemistry were used to evaluate the histological and immunophenotypic characteristics in 5 MNT cases. Epstein-Barr virus (EBV) status was detected by in situ hybridization for EBV-encoded small RNA (EBER). Polymerase chain reaction was used to detect the rearrangement of immunoglobulin genes.@*Results@#Five cases were MNT, including 3 male and 2 female patients, mean aged 59 years (from 43 to 63 years). All patients had ananterior mediastinal mass, with no myasthenia gravis and autoimmune diseases, and underwent surgical resection.Half to ten years follow-up showed no recurrence.Grossly, the tumors were solid in 4 cases, and cystic and solid in 1 case; the border was clear. Histologically, the tumors presented as a distribution of micronodules separated by abundant lymphoid stroma with prominent germinal centers. The nodules were composed of neoplastic spindle, oval cells containing bland, oval nuclei.Immunohistochemical study showed strong positivity of the tumor cells for CKpan, CK19, CK5/6 and p63. Stains for EMA, CD117, calretinin, TTF1 were negative in the tumor cells.Scattered CD3, CD1a, and TdT positive immature T lymphocytes were noted in and around tumor nodules. Many lymphocytes in the stroma, including germinal centers, were positive for CD20.The bcl-2 was also detected in lymphocytes in the stroma, mantle and marginal zone of lymphoid follicles, and in part of tumor cells. Tumor cells and lymphocytes were negative for EBER. Immunoglobulin genes rearrangement analysis showed that B lymphocytes were polyclonal.@*Conclusions@#MNT is a rare thymoma, which occurs in the elderly and has no obvious symptom. After complete resection, the prognosis is very good. The diagnosis should be based on a combination of clinicopathologic features, and other types of thymoma should be excluded.

Evaluation of the effect of emergency nursing pathway applied in the treatment of patients with questionable sources / 中国实用护理杂志

Objective To investigate the effect of emergency nursing pathway in emergency treatment of patients with questionable sources. Methods A clinical nursing pathway for the treatment for patients with questionable sources was formulated. From February to December 2014, 78 patients with questionable sources treated by clinical nursing pathway were selected as the observation group. From January to November 2013, 75 patients with questionable sources treated by routine nursing care methods were selected as the control group. Differences were compared in duration of staying in the emergency department, nursing complication and fees paid rate between the two groups. Results For the average duration of staying in the emergency department, the observation group was lower than that in the control group [19.35%(9.08) vs. 19.90%(25.20), P< 0.05)]. For the nursing complication rate, the observation group was significantly lower than that of the control group [1.28%(1/78) vs.10.67%(8/75), P<0.05]. For the fees paid rate, the observation group was significantly higher than that in the control group [64.10%(50/78) vs. 33.33%(25/75), P<0.01]. Conclusions By the emergency nursing pathway, emergent patients with questionable sources will be treated with satisfactory result in the shortest time.