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1.
FEBS Lett ; 228(2): 301-4, 1988 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-3342884

RESUMO

The cDNA that encodes goat growth hormone (gGH) was isolated from a goat pituitary cDNA library. The cDNA, about 880 base pairs long, had a coding sequence, 5'- and 3'-untranslated regions and a poly(A) chain. The cDNA could encode a polypeptide of 217 amino acids. The amino acid sequence homology between gGH and the sequences of bovine GH, rat GH and human GH was 99, 83 and 66%, respectively. By Northern blot hybridization, we found that the possible gGH gene is transcribed in the goat pituitary.


Assuntos
Clonagem Molecular , DNA/genética , Cabras/genética , Hormônio do Crescimento/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA Recombinante , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Hipófise/análise , RNA Mensageiro/genética , Homologia de Sequência do Ácido Nucleico
2.
J Biochem ; 116(2): 374-9, 1994 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-7822257

RESUMO

Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine; PAF) acetyl-hydrolase is an enzyme that hydrolyzes the acetyl ester of PAF. We purified this enzyme, which accumulated in the peritoneal cavity during endotoxin shock, by ammonium sulfate precipitation, and sequential use of butyl-Toyopearl, heparin-Sepharose, Con A-Sepharose, chelating-Sepharose, and MonoQ column chromatographies. We identified a monomeric polypeptide with a molecular weight of approximately 63 kDa on SDS-PAGE. This molecular weight differs from those of previously described acetylhydrolases. The purified enzyme did not degrade phospholipids with a long chain fatty acyl group at the sn-2 position. In addition, the enzyme activity was not inhibited by either pBPB or quinacrine. Accordingly, this enzyme is distinct from phospholipase A2. In addition, this enzyme also hydrolyzed some oxidatively fragmented phospholipids with PAF-like biological activities such as 1-O-hexadecyl-2-glutaroyl-sn-glycero-3-phosphocholine and 1-O-hexadecyl-2-succinoyl-sn-glycero-3-phosphocholine.


Assuntos
Líquido Ascítico/enzimologia , Fosfolipases A/isolamento & purificação , Fosfolipases A/metabolismo , Choque Séptico/enzimologia , 1-Alquil-2-acetilglicerofosfocolina Esterase , Animais , Cromatografia/métodos , Cobaias , Hidrolases/antagonistas & inibidores , Hidrolases/metabolismo , Hidrólise , Masculino , Organofosfatos/farmacologia , Oxirredução , Fluoreto de Fenilmetilsulfonil/farmacologia , Fosfolipases A/antagonistas & inibidores , Fosfolipases A2 , Fosfolipídeos/metabolismo , Especificidade por Substrato
3.
Artigo em Inglês | MEDLINE | ID: mdl-7584717

RESUMO

The locomotor activities induced by methamphetamine (MAP: 1 mg/kg) following the microinjection of either GABA or three synthesized GABA-peptides (PLG, PSLG, PDSLG) into the rat caudate putamen or the amygdala were measured by using behavioral analysis. The ip administration of MAP induced hyperactivities in a time-dependent manner, and the maximum activity was measured 30 min after MAP administration. This hyperactivity was observed for more than 2 hrs. By the microinjection of GABA-peptides (0.054-540 nmol) into each brain region, the MAP-induced hyperactivity was significantly reduced in a dose-dependent manner, although the single administration of examined dosages of these peptides did not influence the locomotor activity. In the case of microinjection of GABA into either one of the brain regions, only the larger dosage (27,000 nmol) significantly reduced the MAP-induced hyperactivity, whereas this inhibition was less than that seen at the dose of 540 nmol of GABA-peptides. Furthermore, the microinjection of newly synthesized GABA-peptides, PSLG and PDSLG, into the caudate putamen was more effective than those obtained by injection into the amygdala. However, either microinjection of PLG or GABA into both regions showed similar inhibition. These results suggest that the pharmacological properties of newly synthesized GABA-peptides are different compared to those of PLG and GABA, and that these differences might be due to the primary inhibitory effects of the serine-contained GABA-peptides on dopaminergic neuronal activity, but not on GABA neurons.


Assuntos
Moduladores GABAérgicos/farmacologia , Hipercinese/induzido quimicamente , Peptídeos/farmacologia , Tonsila do Cerebelo , Animais , Relação Dose-Resposta a Droga , Moduladores GABAérgicos/administração & dosagem , Masculino , Metanfetamina/antagonistas & inibidores , Microinjeções , Peptídeos/administração & dosagem , Putamen , Ratos , Ratos Wistar , Fatores de Tempo
4.
Chem Pharm Bull (Tokyo) ; 45(12): 1945-54, 1997 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-9433764

RESUMO

A series of 2-aryl-4,5-dihydro-1H-thieno[3,2-e]benzimidazoles (1, 2) was prepared by condensation of 5-acylamino-4,5,6,7-tetrahydrobenzo[b]thiophen-4-ones (9, 10) with ammonium acetate under azeotropic reaction conditions. Various congeners, N-methyl and N-phenyl analogues (3-5), 4,5-dihydro-1H-thieno[2,3-e]benzimidazoles (6), 4,5-dihydro-1H-thieno[2,3-g]benzoxazoles (7), and 4,5-dihydro-1H-thieno[2,3-g]benzothiazoles (8), were also prepared. Several compounds in this series were shown to be K(+)-competitive inhibitors of the gastric (H+/K+)-ATPase and more potent inhibitors than SK&F-96067, 3-butyryl-8-methoxy-4-(2-tolylamino)quinoline, on pentagastrin-stimulated acid secretion in chronic gastric fistula rats after intraduodenal administration.


Assuntos
Benzimidazóis/síntese química , Inibidores Enzimáticos/síntese química , Fundo Gástrico/enzimologia , Inibidores da Bomba de Prótons , Animais , Benzimidazóis/química , Benzimidazóis/farmacologia , Modelos Animais de Doenças , Duodeno , Ácido Gástrico/metabolismo , Fístula Gástrica/tratamento farmacológico , Fundo Gástrico/efeitos dos fármacos , Pentagastrina/antagonistas & inibidores , Ratos , Relação Estrutura-Atividade
5.
Eur J Neurol ; 7(2): 227-30, 2000 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10809947

RESUMO

We reported a 64-year-old male with an eight-month history of gait disturbance and sensory impairment. The patient initially noticed unsteadiness of gait and numbness in his feet, and these symptoms progressed until he was unable to walk without assistance five months later. Vibratory sensation and position sense were markedly diminished, and deep tendon reflexes were absent in all extremities. Motor conduction velocities were slow with prolonged distal latencies, and sensory nerve action potentials (SNAP) were not elicited. Sural nerve biopsy revealed a mild loss of myelinated fibres and segmental demyelination. Cerebrospinal fluid showed normal cell count with protein 526 mg/dL. Anti-GM1, anti-GM2 and anti-GA1 antibodies in serum were positive. We diagnosed chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) presenting ataxia. Steroid therapy provided immediate improvement of symptoms and signs. This case suggests that CIDP should be considered as one of the potential causes of ataxic neuropathy.


Assuntos
Ataxia/fisiopatologia , Polirradiculoneuropatia Desmielinizante Inflamatória Crônica/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Condução Nervosa/fisiologia
6.
Eur Neurol ; 45(4): 241-8, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11385262

RESUMO

We investigated clinical and pathological features of the sural nerves of 5 patients with the ataxic form of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) and compared these features with those of chronic ataxic neuropathies due to other causes. The CIDP patients presented with slowly progressive ataxia with deep sensory impairment. The durations of the symptoms from onset were relatively short in CIDP (4-8 months) and cancer (3 and 10 months), but long in chronic idiopathic ataxic neuropathy (24-260 months). Corticosteroid therapy elicited a good response in all the patients with CIDP, but a poor response in the patients with other ataxic neuropathies. Sural nerve biopsy of CIDP patients showed a slight or moderate loss of myelinated fiber. This report suggests that ataxic form of CIDP is a steroid-responsive ataxic neuropathy, and large myelinated fibers of the sural nerves in ataxic form of CIDP were better preserved than those in nerves with other chronic ataxic neuropathies.


Assuntos
Ataxia/patologia , Ataxia/fisiopatologia , Polirradiculoneuropatia Desmielinizante Inflamatória Crônica/patologia , Polirradiculoneuropatia Desmielinizante Inflamatória Crônica/fisiopatologia , Nervo Sural/patologia , Nervo Sural/fisiopatologia , Adulto , Idoso , Anti-Inflamatórios/uso terapêutico , Ataxia/tratamento farmacológico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fibras Nervosas Mielinizadas/patologia , Fibras Nervosas Mielinizadas/fisiologia , Polirradiculoneuropatia Desmielinizante Inflamatória Crônica/tratamento farmacológico , Prednisolona/uso terapêutico
7.
Arch Biochem Biophys ; 340(2): 185-94, 1997 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-9143320

RESUMO

A type II ribosome-inactivating protein (RIP) was isolated from the bark tissue of Japanese elderberry (Sambucus sieboldiana) and named sieboldin-b. Sieboldin-b is a heterodimeric protein consisting of 27- and 33-kDa subunits and showed strong ribosome-inactivating activity in vitro but did not show in vivo toxicity. The amino acid sequence of sieboldin-b deduced from the structure of the cDNA showed that both subunits of sieboldin-b are encoded on a single precursor polypeptide. Sieboldin-b has a structure homologous with the Neu5Ac(alpha 2-6)Gal/GalNAc-specific bark lectin from S. sieboldiana (SSA) and also typical type II RIPs such as ricin and abrin. Detailed analyses of carbohydrate binding properties of sieboldin-b revealed that sieboldin-b binds to Gal/GalNAc, similar to ricin/abrin, in spite of its highly homologous structure with SSA. The biological properties of these toxins/lectins are compared, and the possible explanation for such diversity is discussed.


Assuntos
Lectinas/química , N-Glicosil Hidrolases/genética , Inibidores da Síntese de Proteínas , Sequência de Aminoácidos , Metabolismo dos Carboidratos , Clonagem Molecular , DNA Complementar/genética , Genes de Plantas , Células HeLa , Humanos , Lectinas/genética , Lectinas/metabolismo , Dados de Sequência Molecular , Peso Molecular , Lectinas de Plantas , Plantas/genética , RNA de Plantas , Proteínas Inativadoras de Ribossomos
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