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AIM: This study aimed to explore the value of ultrasound (US) images in chronic kidney disease (CKD) screening by constructing a CKD screening model based on grey-scale US images. METHODS: According to the CKD diagnostic criteria, 1049 patients from Tongde Hospital of Zhejiang Province were retrospectively enrolled in the study. A total of 4365 renal US images were collected from these patients. Convolutional neural networks were used for feature extractions and a screening model was constructed by fusing ResNet34 and texture features to identify CKD and its stage. A comparative analysis was performed to compare the diagnosis results of the model with physicians. RESULTS: When diagnosing CKD or non-CKD, the receiver operating characteristic curve (AUC) of our model was 0.918 and that of the senior physician group was 0.869 (p < .05). For the diagnosis of CKD stage, the AUC of our model for CKD G1-G3 was 0.781, 0.880, and 0.905, respectively, while the AUC of the senior physician group for CKD G1-G3 was 0.506, 0.586, and 0.796, respectively; all differences were statistically significant (p < .05). The diagnostic efficiency of our model for CKD G4 and G5 reached the level of the senior physicians group. Specifically, the AUC of our model for CKD G4-G5 was 0.867 and 0.931, respectively, while the AUC of the senior physician group for CKD G4-G5 was 0.838 and 0.963, respectively (all p > .05). CONCLUSIONS: Our deep learning radiomics model is more effective than senior physicians in the diagnosis of early CKD.
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Most bacteria use type II fatty acid synthesis (FAS) systems for synthesizing fatty acids, of which the conserved FabA-FabB pathway is considered to be crucial for unsaturated fatty acid (UFA) synthesis in gram-negative bacteria. Xanthomonas campestris pv. campestris, the phytopathogen of black rot disease in crucifers, produces higher quantities of UFAs under low-temperature conditions for increasing membrane fluidity. The fabA and fabB genes were identified in the X. campestris pv. campestris genome by BLAST analysis; however, the growth of the X. campestris pv. campestris fabA and fabB deletion mutants was comparable to that of the wild-type strain in nutrient and minimal media. The X. campestris pv. campestris ΔfabA and ΔfabB strains produced large quantities of UFAs and, altogether, these results indicated that the FabA-FabB pathway is not essential for growth or UFA synthesis in X. campestris pv. campestris. We also observed that the expression of X. campestris pv. campestris fabA and fabB restored the growth of the temperature-sensitive Escherichia coli fabA and fabB mutants CL104 and CY242, respectively, under non-permissive conditions. The in-vitro assays demonstrated that the FabA and FabB proteins of X. campestris pv. campestris catalyzed FAS. Our study also demonstrated that the production of diffusible signal factor family signals that mediate quorum sensing was higher in the X. campestris pv. campestris ΔfabA and ΔfabB strains and greatly reduced in the complementary strains, which exhibited reduced swimming motility and attenuated host-plant pathogenicity. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
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Xanthomonas campestris , Xanthomonas campestris/metabolismo , Ácidos Graxos/metabolismo , Escherichia coli/genética , Percepção de Quorum , Ácidos Graxos Insaturados/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismoRESUMO
A Rydberg atom-based mixer has opened up a new method to characterize microwave electric fields such as the precise measurement of their phase and strength. This study further demonstrates, theoretically and experimentally, a method to accurately measure the polarization of a microwave electric field based on a Rydberg atom-based mixer. The results show that the amplitude of the beat note changes with the polarization of the microwave electric field in a period of 180 degrees, and in the linear region a polarization resolution better than 0.5 degree can be easily obtained which reaches the best level by a Rydberg atomic sensor. More interestingly, the mixer-based measurements are immune to the polarization of the light field that forms the Rydberg EIT. This method considerably simplifies theoretical analysis and the experimental system required for measuring microwave polarization using Rydberg atoms and is of interest in microwave sensing.
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BACKGROUND: Storage affects rice quality significantly. The aim of this study was to investigate the changes in the chemical composition and in the physiological and cooking quality characteristics of three rice types after 1 year storage at 25 °C. RESULTS: Two japonica, two indica, and two indica-japonica hybrid rice varieties were selected. After storage, the total starch content decreased. The amylose content of japonica, indica, and indica-japonica hybrid rice increased by 9.63%-11.65%, 2.99%-4.67%, and 8.07%-8.97%, respectively, and the fat content decreased by 60.00%-65.00%, 37.21%-46.51%, and 41.67%-42.42%, respectively. The abscisic acid (ABA) and raffinose content decreased after 1 year's storage; the former decreased gradually during the storage and the latter increased by 19.35%-45.45%, 7.02%-10.77%, and 16.13%-28.13%, respectively, after 4 months' storage and then decreased to the lowest level after 1 year's storage. The activity of antioxidant enzymes deceased, which resulted in the increases in fatty acid value and malondialdehyde (MDA). The changes in chemical composition after 1 year storage led to the deterioration of rice cooking quality, which was reflected in the decrease in viscosity and increases in gelatinization temperature and cooked rice hardness. CONCLUSION: After 1 year's storage, the rice chemical composition changed and physiological and cooking quality characteristics decreased. Compared with japonica and indica-japonica hybrid rice, indica rice was more stable during 1 year storage. This may be due to the higher content of ABA and raffinose in fresh rice. Our findings will provide information for the identification and breeding of storable rice cultivars. © 2022 Society of Chemical Industry.
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Oryza , Oryza/química , Rafinose , Melhoramento Vegetal , Amido/química , Culinária/métodos , Amilose/químicaRESUMO
In Xanthomonas spp., the biosynthesis of the yellow pigment xanthomonadin and fatty acids originates in the type II polyketide synthase (PKS II) and fatty acid synthase (FAS) pathways, respectively. The acyl carrier protein (ACP) is the central component of PKS II and FAS and requires posttranslational phosphopantetheinylation to initiate these pathways. In this study, for the first time, we demonstrate that the posttranslational modification of ACPs in X. campestris pv. campestris is performed by an essential 4'-phosphopantetheinyl transferase (PPTase), XcHetI (encoded by Xc_4132). X. campestris pv. campestris strain XchetI could not be deleted from the X. campestris pv. campestris genome unless another PPTase-encoding gene such as Escherichia coli acpS or Pseudomonas aeruginosa pcpS was present. Compared with wild-type strain X. campestris pv. campestris 8004 and mutant XchetI::PapcpS, strain XchetI::EcacpS failed to generate xanthomonadin pigments and displayed reduced pathogenicity for the host plant, Brassica oleracea. Further experiments showed that the expression of XchetI restored the growth of E. coli acpS mutant HT253 and, when a plasmid bearing XchetI was introduced into P. aeruginosa, pcpS, which encodes the sole PPTase in P. aeruginosa, could be deleted. In in vitro enzymatic assays, XcHetI catalyzed the transformation of 4'-phosphopantetheine from coenzyme A to two X. campestris pv. campestris apo-acyl carrier proteins, XcAcpP and XcAcpC. All of these findings indicate that XcHetI is a surfactin PPTase-like PPTase with a broad substrate preference. Moreover, the HetI-like PPTase is ubiquitously conserved in Xanthomonas spp., making it a potential new drug target for the prevention of plant diseases caused by Xanthomonas.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
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Xanthomonas campestris , Xanthomonas , Proteína de Transporte de Acila/genética , Proteína de Transporte de Acila/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Escherichia coli/genética , Pseudomonas aeruginosa/metabolismo , Transferases (Outros Grupos de Fosfato Substituídos)/genética , Transferases (Outros Grupos de Fosfato Substituídos)/metabolismo , Xanthomonas/genética , Xanthomonas/metabolismo , Xanthomonas campestris/metabolismoRESUMO
Heterointerfaces have led to the discovery of novel electronic and magnetic states because of their strongly entangled electronic degrees of freedom. Single-phase chromium compounds always exhibit antiferromagnetism following the prediction of the Goodenough-Kanamori rules. So far, exchange coupling between chromium ions via heteroanions has not been explored and the associated quantum states are unknown. Here, we report the successful epitaxial synthesis and characterization of chromium oxide (Cr_{2}O_{3})-chromium nitride (CrN) superlattices. Room-temperature ferromagnetic spin ordering is achieved at the interfaces between these two antiferromagnets, and the magnitude of the effect decays with increasing layer thickness. First-principles calculations indicate that robust ferromagnetic spin interaction between Cr^{3+} ions via anion-hybridization across the interface yields the lowest total energy. This work opens the door to fundamental understanding of the unexpected and exceptional properties of oxide-nitride interfaces and provides access to hidden phases at low-dimensional quantum heterostructures.
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Social-network-based recommendation algorithms leverage rich social network information to alleviate the problem of data sparsity and boost the recommendation performance. However, traditional social-network-based recommendation algorithms ignore high-order collaborative signals or only consider the first-order collaborative signal when learning users' and items' latent representations, resulting in suboptimal recommendation performance. In this paper, we propose a graph neural network (GNN)-based social recommendation model that utilizes the GNN framework to capture high-order collaborative signals in the process of learning the latent representations of users and items. Specifically, we formulate the representations of entities, i.e., users and items, by stacking multiple embedding propagation layers to recursively aggregate multi-hop neighborhood information on both the user-item interaction graph and the social network graph. Hence, the collaborative signals hidden in both the user-item interaction graph and the social network graph are explicitly injected into the final representations of entities. Moreover, we ease the training process of the proposed GNN-based social recommendation model and alleviate overfitting by adopting a lightweight GNN framework that only retains the neighborhood aggregation component and abandons the feature transformation and nonlinear activation components. The experimental results on two real-world datasets show that our proposed GNN-based social recommendation method outperforms the state-of-the-art recommendation algorithms.
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Algoritmos , Redes Neurais de Computação , Aprendizagem , Registros , Rede SocialRESUMO
Medical audio classification for lung abnormality diagnosis is a challenging problem owing to comparatively unstructured audio signals present in the respiratory sound clips. To tackle such challenges, we propose an ensemble model by incorporating diverse deep neural networks with attention mechanisms for undertaking lung abnormality and COVID-19 diagnosis using respiratory, speech, and coughing audio inputs. Specifically, four base deep networks are proposed, which include attention-based Convolutional Recurrent Neural Network (A-CRNN), attention-based bidirectional Long Short-Term Memory (A-BiLSTM), attention-based bidirectional Gated Recurrent Unit (A-BiGRU), as well as Convolutional Neural Network (CNN). A Particle Swarm Optimization (PSO) algorithm is used to optimize the training parameters of each network. An ensemble mechanism is used to integrate the outputs of these base networks by averaging the probability predictions of each class. Evaluated using respiratory ICBHI, Coswara breathing, speech, and cough datasets, as well as a combination of ICBHI and Coswara breathing databases, our ensemble model and base networks achieve ICBHI scores ranging from 0.920 to 0.9766. Most importantly, the empirical results indicate that a positive COVID-19 diagnosis can be distinguished to a high degree from other more common respiratory diseases using audio recordings, based on the combined ICBHI and Coswara breathing datasets.
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COVID-19 , Algoritmos , COVID-19/diagnóstico , Teste para COVID-19 , Humanos , Pulmão , Redes Neurais de ComputaçãoRESUMO
Fast iterative soft threshold algorithm (FISTA) is one of the algorithms for the reconstruction part of compressed sensing (CS). However, FISTA cannot meet the increasing demands for accuracy and efficiency in the signal reconstruction. Thus, an improved algorithm (FIPITA, fast iterative parametric improved threshold algorithm) based on mended threshold function, restart adjustment mechanism and parameter adjustment is proposed. The three parameters used to generate the gradient in the FISTA are carefully selected by assessing the impact of them on the performance of the algorithm. The developed threshold function is used to replace the soft threshold function to reduce the reconstruction error and a restart mechanism is added at the end of each iteration to speed up the algorithm. The simulation experiment is carried out on one-dimensional signal and the FISTA, RadaFISTA and RestartFISTA are used as the comparison objects, with the result that in one case, for example, the residual rate of FIPITA is about 6.35% lower than those three and the number of iterations required to achieve the minimum error is also about 102 less than that of FISTA.
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In this research, we propose two Particle Swarm Optimisation (PSO) variants to undertake feature selection tasks. The aim is to overcome two major shortcomings of the original PSO model, i.e., premature convergence and weak exploitation around the near optimal solutions. The first proposed PSO variant incorporates four key operations, including a modified PSO operation with rectified personal and global best signals, spiral search based local exploitation, Gaussian distribution-based swarm leader enhancement, and mirroring and mutation operations for worst solution improvement. The second proposed PSO model enhances the first one through four new strategies, i.e., an adaptive exemplar breeding mechanism incorporating multiple optimal signals, nonlinear function oriented search coefficients, exponential and scattering schemes for swarm leader, and worst solution enhancement, respectively. In comparison with a set of 15 classical and advanced search methods, the proposed models illustrate statistical superiority for discriminative feature selection for a total of 13 data sets.
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We herein develop and demonstrate a stable frequency-locking scheme for Rydberg atomic experiments. We use the Zeeman effect to modulate the three-level ladder-type Rydberg electromagnetically induced transparency (EIT) signal to lock the laser frequency of the coupling light for transition from its intermediate state to a Rydberg state. The effects of polarization of the probe and coupling lights, and the amplitude of the AC modulated magnetic field ${{\boldsymbol B}_0}$B0 on the EIT and the corresponding dispersive error signal, are both analyzed. The results show that both the EIT signal and dispersive error signal are the strongest when the polarizations of coupling and probe fields are circular and equal. The signal-to-noise ratio of the dispersive error signal increases with ${{\boldsymbol B}_0}$B0. The slope of the dispersive error signal increases first and then decreases with ${{\boldsymbol B}_0}$B0, which is related to the increase of the EIT linewidth caused by the higher ${{\boldsymbol B}_0}$B0. The linewidth of the laser is significantly less than 500 kHz after frequency locking, which satisfies the requirements of most experiments involving Rydberg atoms. The method proposed herein can generally be applied to any cascade system of Rydberg atoms.
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We herein developed and demonstrated a Zeeman frequency modulation scheme for improving the signal-to-noise ratio of microwave electric field measurement using Rydberg atoms. The spectra of the electromagnetically induced transparency (EIT) and Autler-Townes splitting of Rydberg atoms is frequency modulated by an alternating current magnetic field. The signal-to-noise ratio of the corresponding dispersive error signal is enhanced more than 10 times than that of the original spectrum. Furthermore, we show that the slope of the dispersive error signal near the resonance of the Rydberg EIT can be used to characterize the weak microwave electric field amplitudes. The more intuitive and simpler structure compared with other existing frequency modulation technologies greatly reduces the difficulties of experiments and experimental data analysis.
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OBJECTIVE: The objective of this study was to assess left atrial (LA) function with two-dimensional speckle tracking echocardiography (2DSTE) in addition to standard echocardiographic assessments in patients with hypertrophic obstructive cardiomyopathy (HOCM) before and 1 year after septal ablation (SA). METHODS: The study included 31 patients with HOCM, who underwent SA. Each patient with HOCM underwent a complete two-dimensional transthoracic echocardiography before and 1 year after the SA. The measurements included basal septal thickness, left ventricular outflow trace (LVOT) gradient, mitral regurgitation (MR) grade, LA dimensions, left ventricular (LV) ejection fraction, and tissue Doppler parameters of lateral mitral annular e' and septal mitral annular e'. The LA wall was tracked on a frame-by-frame basis using 2DSTE, and LA volume waveforms were generated. The maximum LA volume (LAVmax ), minimal LA volume (LAVmin ), and the LA volume before atrial contraction (LAVpre-a ) were measured. The LA reservoir function was calculated as the expansion index and diastolic emptying index. The LA conduit function was calculated as the passive emptying percentage of total emptying (PE) and the passive emptying index (PEI). The LA booster function was calculated as the active emptying percentage of total emptying (AE) and the active emptying index (AEI). RESULTS: The LVOT gradient, end-diastolic septal base thickness, the grade of MR, and LA end-diastolic size were significantly decreased in patients with HOCM before and 1 year after the SA (All P<.05). The lateral mitral annular e' was significantly increased (P<.05), and the E/lateral e' ratio was significantly decreased (P<.05), whereas septal mitral annular e' was significantly decreased (P<.05), and the E/septal e' ratio was significantly increased (P<.05). LAVImax , LAVImin , and LAVIpre-a were significantly decreased 1 year after the SA (All P<.05). The expansion and diastolic emptying indices were significantly increased (All P<.05) at 1 year after the SA. PE and PEI were significantly increased (All P<.05). The AE and AEI were significantly decreased (All P<.05). LAVImax , LAVImin , and LAVIpre-a of the responders group were significantly lower than those of the nonresponders group (All P<.05). The expansion and diastolic emptying indices of the responders group were significantly higher than those of the nonresponders group (All P<.05). The PE and PEI of the responders were significantly higher than those of the nonresponders group (All P<.05). The AE and AEI of the responders were significantly lower than those of the nonresponders group (All P<.05). CONCLUSIONS: We found an improvement in the LA reservoir and conduit function but a reduction in LA booster pump function 1 year after the SA in the responders.
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Função do Átrio Esquerdo/fisiologia , Cardiomiopatia Hipertrófica/cirurgia , Ablação por Cateter , Ecocardiografia/métodos , Complicações Pós-Operatórias/diagnóstico por imagem , Cardiomiopatia Hipertrófica/fisiopatologia , Feminino , Seguimentos , Átrios do Coração/diagnóstico por imagem , Átrios do Coração/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/fisiopatologiaRESUMO
In Xanthomonas campestris pv. campestris (Xcc), the proteins encoded by the rpf (regulator of pathogenicity factor) gene cluster produce and sense a fatty acid signal molecule called diffusible signalling factor (DSF, 2(Z)-11-methyldodecenoic acid). RpfB was reported to be involved in DSF processing and was predicted to encode an acyl-CoA ligase. We report that RpfB activates a wide range of fatty acids to their CoA esters in vitro. Moreover, RpfB can functionally replace the paradigm bacterial acyl-CoA ligase, Escherichia coliâ FadD, in the E. coli ß-oxidation pathway and deletion of RpfB from the Xcc genome results in a strain unable to utilize fatty acids as carbon sources. An essential RpfB function in the pathogenicity factor pathway was demonstrated by the properties of a strain deleted for both the rpfB and rpfC genes. The ΔrpfB ΔrpfC strain grew poorly and lysed upon entering stationary phase. Deletion of rpfF, the gene encoding the DSF synthetic enzyme, restored normal growth to this strain. RpfF is a dual function enzyme that synthesizes DSF by dehydration of a 3-hydroxyacyl-acyl carrier protein (ACP) fatty acid synthetic intermediate and also cleaves the thioester bond linking DSF to ACP. However, the RpfF thioesterase activity is of broad specificity and upon elimination of its RpfC inhibitor RpfF attains maximal activity and its thioesterase activity proceeds to block membrane lipid synthesis by cleavage of acyl-ACP intermediates. This resulted in release of the nascent acyl chains to the medium as free fatty acids. This lack of acyl chains for phospholipid synthesis results in cell lysis unless RpfB is present to counteract the RpfF thioesterase activity by catalysing uptake and activation of the free fatty acids to give acyl-CoAs that can be utilized to restore membrane lipid synthesis. Heterologous expression of a different fatty acid activating enzyme, the Vibrio harveyi acyl-ACP synthetase, replaced RpfB in counteracting the effects of high level RpfF thioesterase activity indicating that the essential role of RpfB is uptake and activation of free fatty acids.
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Coenzima A Ligases/metabolismo , Ácidos Graxos/metabolismo , Xanthomonas campestris/enzimologia , Acil Coenzima A/metabolismo , Proteínas de Bactérias/metabolismo , Carbono-Enxofre Ligases/metabolismo , Coenzima A Ligases/genética , Coenzima A Ligases/isolamento & purificação , Difusão , Escherichia coli/genética , Escherichia coli/crescimento & desenvolvimento , Proteínas de Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos , Teste de Complementação Genética , Enzimas Multifuncionais/metabolismo , Família Multigênica , Mutação , Oxirredução , Doenças das Plantas/microbiologia , Transdução de Sinais/genética , Especificidade por Substrato , Tioléster Hidrolases/metabolismo , Xanthomonas campestris/genética , Xanthomonas campestris/crescimento & desenvolvimento , Xanthomonas campestris/patogenicidadeAssuntos
Neoplasias dos Ductos Biliares/diagnóstico por imagem , Neoplasias dos Ductos Biliares/patologia , Colangiocarcinoma/diagnóstico por imagem , Colangiocarcinoma/patologia , Ultrassonografia Doppler em Cores , Idoso , Idoso de 80 Anos ou mais , Ductos Biliares Intra-Hepáticos , Meios de Contraste , Erros de Diagnóstico , Feminino , Humanos , Abscesso Hepático/diagnóstico por imagem , Masculino , Necrose/diagnóstico por imagem , Necrose/patologia , Tomografia Computadorizada por Raios X , Carga Tumoral , Ultrassonografia Doppler em Cores/métodosRESUMO
Benign metastasizing leiomyoma (BML) is a rare disease that results from metastasis of uterine leiomyoma to distant sites with benign pathologic features. The lung is the most common metastatic site for BML. This report describes the case of a 49-year-old woman who presented with a mass in the abdominal wall with a surgical history of uterine myomectomy. Ultrasound and Magnetic resonance imaging (MRI) revealed multiple mass lesions. The histopathology of the mass specimen indicated BML. The imaging and clinical features of BML are discussed based on the characteristics of this case and related literature reports.
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The genotype of rice variety is important factor that affect grain quality during storage. In this study, japonica, indica, and indica-japonica hybrid rice were selected to reveal the changes in amylase activities, starch fine structure, and physicochemical properties after one year of storage. Amylase activities decreased after storage and starch molecules were degraded, which, respectively, decreased and increased the content of the amylopectin long chains (DP 37+) and amylose. Changes in the starch fine structure resulted in lower swelling factors and a higher gelatinization temperature. Compared with japonica and indica-japonica hybrid rice, indica rice had a more stable starch fine structure and physicochemical properties after storage. The information obtained from this study provides a better understanding of the changes that occur in starch at the molecular level during storage and will provide insight into the breeding of storable rice varieties.
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Oryza , Amido , Amido/química , Oryza/química , Amilopectina/química , Amilose/química , Amilases/metabolismoRESUMO
Population growth and economic development in China has increased the demand for food and animal feed, raising questions regarding China's future maize production self-sufficiency. Here, we address this challenge by combining data-driven projections with a machine learning method on data from 402 stations, with data from 87 field experiments across China. Current maize yield would be roughly doubled with the implementation of optimal planting density and management. In the 2030 s, we estimate a 52% yield improvement through dense planting and soil improvement under a high-end climate forcing Shared Socio-Economic Pathway (SSP585), compared with a historical climate trend. Based on our results, yield gains from soil improvement outweigh the adverse effects of climate change. This implies that China can be self-sufficient in maize by using current cropping areas. Our results challenge the view of yield stagnation in most global areas and provide an example of how food security can be achieved with optimal crop-soil management under future climate change scenarios.
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Solo , Zea mays , Produtos Agrícolas , China , Ração Animal , Mudança Climática , Agricultura/métodos , Produção AgrícolaRESUMO
Semantic segmentation is vital for many emerging surveillance applications, but current models cannot be relied upon to meet the required tolerance, particularly in complex tasks that involve multiple classes and varied environments. To improve performance, we propose a novel algorithm, neural inference search (NIS), for hyperparameter optimization pertaining to established deep learning segmentation models in conjunction with a new multiloss function. It incorporates three novel search behaviors, i.e., Maximized Standard Deviation Velocity Prediction, Local Best Velocity Prediction, and n -dimensional Whirlpool Search. The first two behaviors are exploratory, leveraging long short-term memory (LSTM)-convolutional neural network (CNN)-based velocity predictions, while the third employs n -dimensional matrix rotation for local exploitation. A scheduling mechanism is also introduced in NIS to manage the contributions of these three novel search behaviors in stages. NIS optimizes learning and multiloss parameters simultaneously. Compared with state-of-the-art segmentation methods and those optimized with other well-known search algorithms, NIS-optimized models show significant improvements across multiple performance metrics on five segmentation datasets. NIS also reliably yields better solutions as compared with a variety of search methods for solving numerical benchmark functions.