Insights into RNA N6-methyladenosine and programmed cell death in atherosclerosis.

N6-methyladenosine (m6A) modification stands out among various RNA modifications as the predominant form within eukaryotic cells, influencing numerous cellular processes implicated in disease development. m6A modification has gained increasing attention in the development of atherosclerosis and has become a research hotspot in recent years. Programmed cell death (PCD), encompassing apoptosis, autophagy, pyroptosis, ferroptosis, and necroptosis, plays a pivotal role in atherosclerosis pathogenesis. In this review, we delve into the intricate interplay between m6A modification and diverse PCD pathways, shedding light on their complex association during the onset and progression of atherosclerosis. Clarifying the relationship between m6A and PCD in atherosclerosis is of great significance to provide novel strategies for cardiovascular disease treatment.

Direct and Indirect Electron Transfer Routes of Chromium(VI) Reduction with Different Crystalline Ferric Oxyhydroxides in the Presence of Pyrogenic Carbon.

Electron transfer mediated by iron minerals is considered as a critical redox step for the dynamics of pollutants in soil. Herein, we explored the reduction process of Cr(VI) with different crystalline ferric oxyhydroxides in the presence of pyrogenic carbon (biochar). Both low- and high-crystallinity ferric oxyhydroxides induced Cr(VI) immobilization mainly via the sorption process, with a limited reduction process. However, the Cr(VI) reduction immobilization was inspired by the copresence of biochar. Low-crystallinity ferric oxyhydroxide had an intense chemical combination with biochar and strong sorption for Cr(VI) via inner-sphere complexation, leading to the indirect electron transfer route for Cr(VI) reduction, that is, the electron first transferred from biochar to iron mineral through C-O-Fe binding and then to Cr(VI) with Fe(III)/Fe(II) transformation on ferric oxyhydroxides. With increasing crystallinity of ferric oxyhydroxides, the direct electron transfer between biochar and Cr(VI) became the main electron transfer avenue for Cr(VI) reduction. The indirect electron transfer was suppressed in the high-crystallinity ferric oxyhydroxides due to less sorption of Cr(VI), limited combination with biochar, and higher iron stability. This study demonstrates that electron transfer mechanisms involving iron minerals change with the mineral crystallization process, which would affect the geochemical process of contaminants with pyrogenic carbon.

Effect of NaNO3, NH4Cl and urea on the fate and transformation of various typical microplastics in porous media.

Understanding the transport behaviors of microplastics (MPs) in porous media is crucial in controlling MPs pollution. Given nitrogen is one of the most important nutrients in soil and groundwater systems, unclearness of the transport behaviors of microplastics (MPs) under various nitrogen conditions may inhibit the acknowledgment of MPs fate. For this reason, this study innovatively investigates the transport characteristics of four kinds of typical MPs (PVC MPs, PMMA MPs, PET MPs, and PP MPs) under various NaNO3, NH4Cl and urea conditions via column experiments numerical models. The FTIR and XPS analysis were conducted to excavate the transform of MPs. The MPs mobility was generally reduced with the increasing nitrogen concentrations. The polarity and density properties of different MPs played combined roles in transport under similar conditions. Compared to NO3-, NH4+ may neutralize the negative charge of MPs and then restrain their transport in porous media. Urea may coat the surface of MPs and promote the mobility, however, increasing concentrations of urea may result in the interattraction between MPs and porous media via hydrogen-bond and π-π interaction. PET MPs and PP MPs showed barely transform during transport under the tested conditions. Particularly, the chlorines on PVC MPs could react with the amide on urea and produce amidogen, which may improve PVC MPs transport. The N-H and C-N bond also generated on PMMA MPs in presence of urea also may enhance the mobility.

Immune cell-mediated features of atherosclerosis.

Atherosclerosis is a chronic inflammatory disease characterized by innate and adaptive immune responses, which seriously threatens human life and health. It is a primary cause of coronary heart disease, myocardial infarction, and peripheral vascular disease. Research has demonstrated that immune cells are fundamental to the development of atherosclerosis and chronic inflammation. Therefore, it is anticipated that immunotherapy targeting immune cells will be a novel technique in the management of atherosclerosis. This article reviews the growth of research on the regulatory role of immune cells in atherosclerosis and targeted therapy approaches. The purpose is to offer new therapeutic approaches for the control and treatment of cardiovascular illnesses caused by atherosclerosis.

Cotransport of 6PPD-Q and pristine/aged microplastics in porous media: An insight based on transport forms and mechanisms.

The environmental fate and risks of microplastics (MPs) and their associated contaminants have attracted increasing concern in recent years. In this study, the cotransport of six kinds of pristine and aged MPs and the antiager ozonation product N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6PPD-Q) were investigated via a series of batch and transport experiments, and characteristic analysis (e.g., SEM, FTIR and XPS). Generally, pristine MPs exhibit higher adsorption ability than aged MPs due to the hydrophobic interaction. The 6PPD-Q usually exhibited both free moving and bond-MPs moving during transport process in presence of MPs, but none free 6PPD-Q was detected in presence of pristine PP MPs. The mobility of 6PPD-Q was generally facilitated in presence of MPs by bond-MPs moving due to the hydrogen bonding, halogen bonding, π-π interaction (the maximum total mass recovery of 84.11%), which efficiency was influenced with the combined effect of adsorption ability and mobility of MPs. The pristine PVC MPs showed highest facilitation on 6PPD-Q transport. The retained 6PPD-Q in porous media also was released by various MPs with different mass recovery ranged from 15.72% to 56.26% via surface moving of MPs around porous media. Both the dissolved and retained 6PPD-Q decreased the MPs mobility with the minimum mass recovery of 34.02%. Findings from this study contribute to the prediction and assessment of the combined risks of MPs and 6PPD-Q.

Aging behavior of microplastics accelerated by mechanical fragmentation: alteration of intrinsic and extrinsic properties.

Microplastics (MPs) inevitably undergo multiple aging processes during their life cycle in the environment. However, the information regarding the mechanical fragmentation behavior of MPs remained unclear, including the changes in the intrinsic properties of aged MPs, the measurement of aging degree, the underlying mechanism, and the interaction with heavy metals. Here, MPs (PS, PP, PET) were aged by crushing (-CR) and ball-milling (-BM) to simulate mild and severe mechanical fragmentation, respectively. Our results indicated that mechanical fragmentation significantly affected the morphology of MPs. The aging degree of MP-BM was deeper compared to MP-CR owing to smaller particle size, larger specific surface area, poorer heat resistance, better hydrophilicity, and richer oxygen-containing functional groups. The carbonyl index (CI) and O/C ratio were used to measure the aging degree of the two mechanical aging treatments. Besides, the mechanism was proposed and the discrepancy between the two treatments was elaborated from three aspects including the excitation energy source, reaction interface, and reaction dynamics. Furthermore, the extrinsic properties of MPs altered with the increase of aging degree; specifically, the adsorption capacities of heavy metals were enhanced. Meanwhile, it was unveiled that the CI value and O/C ratio played a vital role in estimating the adsorption ability of heavy metals. The findings not only reveal the mechanical fragmentation behavior of MPs but also provide new insights into the assessment of the potential risks of the aged MPs via chemical indexes.

Contrasting effects of dry-wet and freeze-thaw aging on the immobilization of As in As-contaminated soils amended by zero-valent iron-embedded biochar.

Zero-valent iron-embedded biochar (ZVI/BC) is considered as an effective material for arsenic (As) immobilization in soil, but the stability of As after remediation against aging remains unknown. Herein, the effects of dry-wet and freeze-thaw aging on the immobilization of As in two As-contaminated soils amended by ZVI/BC were evaluated. ZVI/BC showed high immobilization capacity for As-contaminated soils with an over 82% decrease of bioavailable As, mainly due to the As-Fe co-precipitation accompanied with ZVI oxidation. The aging of dry-wet and freeze-thaw had an opposite effect on the bioavailability of As. After 35 rounds of dry-wet aging, bioavailable As concentration increased from 1.25-9.50 to 1.83-21.75 mg/kg, because of the oxidation dissolution of ZVI and the formation of mobile reduced As(III). By contrast, the crystallization of amorphous iron with the structural incorporation of sorbed As and the oxidation of As(III) into stable As(V) occurred during the 35 rounds of freeze-thaw aging, leading to the decrease of bioavailable As concentration from 9.50-1.25 to 5.42-0.45 mg/kg. Our results revealed that the stability of soil As after remediation by ZVI/BC varied with the different aging process, which needs more consideration for the long-term soil As immobilization in the different whether areas.

Evolution of redox activity of biochar during interaction with soil minerals: Effect on the electron donating and mediating capacities for Cr(VI) reduction.

Biochar in soil is susceptible to natural aging along with soil minerals, which might alter its electrochemical properties and redox reactions with contaminants. In this study, soluble mineral salts (FeCl3, MnCl2, AlCl3, CaCl2) and clay mineral (kaolinite) were selected to investigate the impact of co-aging with soil minerals on the redox activity of peanut-shell biochar for Cr(VI) reduction. Natural aging for 3-month induced oxidation of biochar with the decrease of reducing moieties, i.e., ‒C‒OH from 26.8-43.7% to 18.4-24.1%. Co-aging with minerals except for Mn(II) further decreased the proportion of ‒C‒OH to 6.94-22.2% because of the interaction between mineral ions and biochar, resulting in the formation of mineral-biochar complex and new minerals, e.g. ß-FeOOH. Due to its reductivity, Mn(II) presented the least decrease or even slight increase of ‒C‒OH while itself was oxidized to Mn(III) and Mn(IV). The decline of ‒C‒OH caused the decrease of Cr(VI) reduction rate constant from 2.18 to 2.47 × 10-2 h-1 for original biochars to 0.71-1.95 × 10-2 h-1 for aged ones, of which co-aging with Fe(III) showed the lowest reduction rate constant among all minerals. The electron mediating capacity of biochar also decreased after aging alone or co-aging with Al, Ca, and kaolinite, while co-aging with Fe(III) and Mn(II) facilitated the electron transfer process, increasing the rate constant by 219.3-1237% due to electron mediation through valence transformation of Fe(III)-Fe(II) and Mn(II)-Mn(III). Given the abundance of soil minerals, it was essential to consider this crucial factor for redox reactions when applying biochar for soil remediation.

Pyrolysis-temperature depended electron donating and mediating mechanisms of biochar for Cr(VI) reduction.

Biochar could be involved in environmentally relevant redox reactions, and their redox-active moieties may change with pyrolysis temperature. In this study, pyrolysis-temperature depended electron donating and mediating ability of biochar for Cr(VI) reduction were evaluated. All biochar derived from peanut shell at 400-800 °C effectively reduced Cr(VI) into Cr(III), and the reduction capability decreased as the pyrolysis temperature increased (400-600 °C), and then increased (600-800 °C). The electron donating moieties transformed from the -OH functional groups at lower pyrolysis temperature (<600 °C) to the functional groups associated with conjugated structure at higher temperature (>600 °C). Biochar could mediate the reduction of Cr(VI) by lactate, with the reduction rates of Cr(VI) increased up to 16.3 and 345 times that by either biochar or lactate alone, respectively. The redox ability of biochar was the premise, but its conductivity was the dominant factor, for the mediating reduction of Cr(VI). The mediation capability of biochar increased with elevated pyrolysis-temperature due to its increased conductivity related to the growth of conjugated clusters and carbon defects. Our results indicated that pyrolysis-temperature affects the formation of functional groups and conjugated carbon structure of biochars which have a distinguishable influence on the electron donating and mediating ability for Cr(VI) reduction.

[Genetic polymorphism of six short tandem repeat loci in the Han population in Hebei province of China].

OBJECTIVE: This article reports a population genetic study on six short tandem repeat(STR) loci, D7S820, D19S253, D12S391, D5S818, D16S539 and D8S1179, in a sample of unrelated Chinese Han individuals(n=122-173) living in Hebei province. METHODS: DNA extraction from blood samples (200 in number) and multiplex amplification of the above six loci were carried out. Using denaturing polyacrylamide gel electrophoresis and silver stain, the authors investigated the distribution of allele frequencies of the six loci in Han population in Hebei province. RESULTS: The STR polymorphisms at all of the six loci were observed in Chinese Han population in Hebei province. The observed heterozygosities of D7S820, D19S253, D12S391, D5S818, D16S539 and D8S1179 were 0.828, 0.757, 0.769, 0.837, 0.785 and 0.852, respectively. The measured values of the power of discrimination (PD) were 0.914, 0.919, 0.940, 0.909, 0.917, 0.944; of the mean exclusion chance(MEC) 0.618, 0.740, 0.801, 0.557, 0.655, 0.696 and of the polymorphism information content (PIC) in Chinese 0.771, 0.760, 0.762, 0.708, 0.776 and 0.794, respectively. CONCLUSION: The genotype distributions of the six STR were in accordance with Hardy-Weinberg equilibrium. The numerical values of the PD and MEC are relatively high in Hebei province, and thus can be of significant application in population genetics and forensic medicine.