RESUMO
Plasmid-mediated quinolone resistance mechanisms in extended spectrum beta-lactamase positive and quinolone resistant Escherichia coli and Klebsiella pneumoniae strains isolated from Ege University Hospital were investigated. The presence of qnrA, qnrB, qnrS, aac(6')-Ib and qepA genes were detected by PCR and the products were sequenced. Clonal relationship of isolates was determined by REP-PCR and mutations in gyrA and parC genes were investigated in representative strains. aac(6')-Ib-cr, qnrB and qnrS genes were detected in both E. coli and K. pneumoniae strains, but qnrA detected only in K. pneumoniae strains. qepA determinant is detected in an E. coli strain first time in Turkey. Mutations were observed in both gyrA and parC genes of all representative nalidixic acid and ciprofloxacin resistant E. coli isolates but no mutation was found in parC genes of E. coli and K. pneumoniae strains that were resistant to only nalidixic acid.
Assuntos
Farmacorresistência Bacteriana , Infecções por Escherichia coli/tratamento farmacológico , Proteínas de Escherichia coli/genética , Escherichia coli/enzimologia , Infecções por Klebsiella/tratamento farmacológico , Plasmídeos/genética , Quinolonas/farmacologia , beta-Lactamases/metabolismo , Escherichia coli/efeitos dos fármacos , Infecções por Escherichia coli/microbiologia , Hospitais , Humanos , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Mutação/genética , Filogenia , Reação em Cadeia da Polimerase , TurquiaRESUMO
This study was carried out using Ixodes ricinus ticks collected during 2005 and 2006 from the Friuli Venezia Giulia (FVG) region in the northeastern part of Italy and an area along the Slovenian side of the western border of Italy. The results indicate that Rickettsia spp. is widely distributed throughout these areas, with the greatest prevalence in the central part of the FVG region. The prevalence of Rickettsia spp. was 4.5% during 2005 and 6.1% during 2006. By sequencing the 16S rRNA gene, we show for the first time the presence of Rickettsia helvetica in I. ricinus ticks in the FVG region and the presence of R. monacensis in ticks in both areas. Furthermore, we detected a sequence with a high homology with that of R. limoniae in a tick obtained from the alpine zone.