Oncogene patterns in breast and ovarian carcinomas.

Nineteen paraffin-embedded breast cancer tissue samples selected for long survival (more than 5 years) were analysed for detecting the amplification of the c-erbB-2 (Her-2/neu) oncogene by Polymerase Chain Reaction (PCR). Strong correlation was elucidated between c-erbB-2 amplification and survival; such correlation was also observed with histopathologic types and nuclear grading. Because of the similarity of the breast and ovarian cancer in the etiology of the diseases, amplification of c-erbB-2, c-myc and Ki-ras genes was examined in 32 ovarian carcinoma samples (stage I-IV). In ovarian carcinomas c-erbB-2 amplification occurred in 34% (11/32) of the fresh tumour samples, and correlation between amplification and clinical staging at P < 0.05 significance level was observed. Amplification of c-myc was detected in 9% (3/32) and none of the tumours showed amplification of Ki-ras.

Screening and pathological diagnosis of asbestosis and mesothelioma: a review.

X-ray screenings of workers employed in asbestos manufacturing in Hungary revealed 255 cases of asbestosis and 3 cases of malignant mesothelioma. The pathological diagnosis of asbestosis is not very difficult. The diagnosis of mesothelioma, however, is fraught with problems. To identify mesothelioma we use histochemical reactions with PAS diastase digestion and immunohistochemical reactions for cytokeratin, vimentin, and CEA. The PAS and CEA reactions are negative but the vimentin and cytokeratin are positive in mesothelioma. Cytogenetic and molecular genetic studies may contribute to the better understanding of the pathogenesis and, thus, open new perspectives in our understanding of mesotheliomas.

Relationship of anthropometrical, physiological and motor attributes to sport-specific skills.

The purpose of this pilot study was to investigate the importance of the athlete's motor capabilities in success in sport. More precisely, the association of anthropometrical and physiological attributes, as well as motor abilities of elite basketball players with play elements of basketball. The subjects were seven elite basketball players. At the end of the competitive season, the anthropometrical and physiological features were measured to establish the physical fitness of the subjects. Both general and sport-specific motor tests were done. The coach estimated the performance of each player during the games of the competitive season. The coach's data sheet incorporated 14 parameters of the game. Regression analyses indicated significant correlation between certain variables of the laboratory tests and the data of the coach's estimation statistics. Knowing these relationships provides us with valuable predictive information about player's capabilities in sport.

Allele loss from large regions of chromosome 17 is common only in certain histological subtypes of ovarian carcinomas.

Using a panel of ten polymorphic markers, we examined the frequency of loss of heterozygosity (LOH) on chromosome 17 in 55 sporadic ovarian tumours. LOH on 17p and 17q was observed to be 50% and 62% respectively. LOH at D17S5 was detected in 24/36 (67%) of malignant cases and in 19/43 (44%) at TP53; the marker D17S855 intragenic to the BRCA1 gene showed allele loss in 50% (20/40) cases. The data presented here suggest that loss of the whole chromosome 17 is a relatively frequent event (30%) in ovarian carcinomas and this observation is especially frequent for serous, transitional cell and anaplastic histological subtypes. Mucinous and endometrioid ovarian tumours showed only short interstitial deletions (4/11, 36%). The overall frequency of the short deletions was relatively low (7/43, 16%) in our panel of carcinomas. Amplification of c-erbB-2/neu oncogene was detected in 32% (11/34) of the carcinomas tested; the gene was amplified only in those histological subtypes in which high incidence of LOH on chromosome 17 was observed, and was associated with advanced stages of the disease. We conclude that different histological types of tumour may have different aetiological mechanisms, and tumour-suppressor genes on chromosome 17 might be associated specifically with serous and transitional cell ovarian carcinomas.