RESUMO
Complete karyotyping of G-stained chromosomes of bone marrow and blood cells was carried out in patients with non-Hodgkin's lymphomas before and after antitumor therapy, in remissions and relapses. Clones with 1q41-43, 2q35, and 6q24-27 deletions were most incident. Their incidence in patients increased after antitumor therapy.
Assuntos
Aberrações Cromossômicas , Linfoma não Hodgkin/genética , Adolescente , Adulto , Idoso , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Células Sanguíneas/citologia , Células da Medula Óssea/citologia , Deleção Cromossômica , Cromossomos Humanos Par 1 , Cromossomos Humanos Par 2 , Cromossomos Humanos Par 4 , Cromossomos Humanos Par 5 , Cromossomos Humanos Par 6 , Feminino , Humanos , Cariotipagem , Linfoma não Hodgkin/sangue , Linfoma não Hodgkin/tratamento farmacológico , Masculino , Pessoa de Meia-IdadeRESUMO
The analysis was performed on 514 blood lymphocytes from a person accidentally exposed to 137Cs. Blood samples were collected 1 year after exposure three times at intervals of one month. Terminal deletions and simple translocations were found to predominate in all cases. No differences between these cases were observed on analysing total frequency of stable chromosome aberrations. However, the frequency of terminal deletions decreased and frequencies of exchange-type aberration increased with time after exposure. Chromosome #4 was more involved in stable aberrations than it would be expected from the relative chromosome lengths. Clonal aberrations del-ter (5)(q31 or 32) were found.
Assuntos
Aberrações Cromossômicas , Lesões por Radiação/genética , Adulto , Bandeamento Cromossômico , Humanos , Linfócitos , Masculino , Lesões por Radiação/sangueRESUMO
After the Chernobyl accident in a period from 1989 to 1992 blood samples were collected and analysed for chromosomal aberrations in lymphocytes from 490 children born and living continuously in two districts of Kaluga Province with radioactive contamination. The results of cytogenetic examinations show the frequency of unstable aberrations in children from Zhizdra District to be higher than that in children from Ulyanovo District and in the control group. No correlation was found between the thyroid absorbed doses ranging from 0 to 5 Gy and the aberration frequencies. No differences in chromosomal aberration yields were observed in children born before or after the Chernobyl accident. Repeat examinations of the same children cohorts revealed no significant changes with time in the level of unstable aberrations.
Assuntos
Aberrações Cromossômicas/genética , Exposição Ambiental/efeitos adversos , Radioisótopos/efeitos adversos , Adolescente , Radioisótopos de Césio/efeitos adversos , Criança , Exposição Ambiental/estatística & dados numéricos , Feminino , Humanos , Masculino , Centrais Elétricas , Liberação Nociva de Radioativos , Federação Russa , Glândula Tireoide/efeitos da radiação , Fatores de Tempo , UcrâniaRESUMO
Within the frames of activity of joint commission the cytogenetic studies of 98 persons from different areas of Semipalatinsk Province have been carried out in 1989. The studies revealed the higher level of chromosome aberrations in the settlements adjacent to firing ground. The possible connection of discovered cytogenetic lesions to the influence of radiation aftermath of ground and air nuclear weapon test is discussed.
Assuntos
Aberrações Cromossômicas/genética , Exposição Ambiental/efeitos adversos , Guerra Nuclear , Cinza Radioativa/efeitos adversos , Adolescente , Adulto , Idoso , Exposição Ambiental/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cinza Radioativa/estatística & dados numéricos , Sibéria , Fatores de TempoRESUMO
The paper presents the data on cytogenetic studies of 663 children inhabited radionuclide contaminated territories of Bryansk Province. Statistically significant difference with control was observed only for the territory with the highest radioactive contamination level of 137Cs (1000 kBq/m2). For two other territories with lesser contamination (100 and 700 kBq/m2) the rate of chromosome aberrations is nearly the same and does not differ significantly from control one. No difference in the level of cytogenetic damage was discovered for children affected by radiation pre- or postnatally.
Assuntos
Aberrações Cromossômicas/genética , Exposição Ambiental/efeitos adversos , Radioisótopos/efeitos adversos , Adolescente , Radioisótopos de Césio/efeitos adversos , Criança , Exposição Ambiental/estatística & dados numéricos , Humanos , Centrais Elétricas , Liberação Nociva de Radioativos , Federação Russa , Fatores de Tempo , UcrâniaRESUMO
Comparative studies of the yield of stable and unstable chromosome aberrations have been carried out in human lymphocytes gamma irradiated in vitro within the doze range 0.1--1.0 Gy. It was observed that the yield of unstable chromosome aberrations (dicentrics and centric rings) is satisfactorily described for discovered dose range with linear-quadratic approximation. For stable chromosome aberrations in G-banded chromosome the dose response dependence was less expressed. The studies revealed non-random involvement of different chromosomes in translocations, and the most frequently involved are 1 and 13 chromosomes.
Assuntos
Aberrações Cromossômicas/genética , Linfócitos/efeitos da radiação , Adulto , Células Cultivadas , Bandeamento Cromossômico , Cromossomos Humanos Par 1/efeitos da radiação , Cromossomos Humanos Par 13/efeitos da radiação , Relação Dose-Resposta à Radiação , Raios gama , Humanos , Cariotipagem , Linfócitos/ultraestrutura , MasculinoAssuntos
Aberrações Cromossômicas/fisiologia , Linfócitos/fisiologia , Previsões , Humanos , PesquisaRESUMO
Non-clonal and clonal abnormal cells (hypodiploid, polyploid, and with chromosome aberrations) were detected by the G-method in the bone marrow and blood of the majority of patients with non-Hodgkin lymphomas before therapy. The manifestation of these abnormalities changed individually after treatment. The most incident were clones -22, del(1)(q42-43), del(2)(q-33-35), del(3)(q27), 4q-, and del(6q).
Assuntos
Células Sanguíneas/metabolismo , Medula Óssea/metabolismo , Citogenética/métodos , Linfoma não Hodgkin/genética , Adolescente , Adulto , Idoso , Aberrações Cromossômicas , Feminino , Humanos , Cariotipagem , Linfoma não Hodgkin/sangue , Linfoma não Hodgkin/terapia , Masculino , Pessoa de Meia-Idade , PoliploidiaRESUMO
A study was made of the frequency of chromosome aberrations in human lymphocyte culture after gamma-irradiation (60Co) with doses ranging from 0.05 to 1.0 Gy at dose--rates of 0,005, 0.05 and 0.5 Gy/min. The frequency of structural changes in chromosomes at low doses was higher than it was expected in the case of extrapolating the effect produced by high to low doses of radiation; within the dose range from 0.1 to 0.5 Gy a plateau was registered for aberrations of the exchange type (dicentrics and rings). The abnormal character of the dose dependence of the yield of chromosome aberrations persisted with all three dose - rates under study.