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1.
Infect Drug Resist ; 17: 3913-3923, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39257441

RESUMO

Introduction: C. psittaci pneumonia has atypical clinical manifestations and is often ignored by clinicians. This study analyzed the clinical characteristics, explored the risk factors for composite outcome and established a prediction model for early prediction of composite outcome among C. psittaci pneumonia patients. Methods: A multicenter, retrospective, observational cohort study was conducted in ten Chinese tertiary hospitals. Patients diagnosed with C. psittaci pneumonia were included, and their clinical data were collected and analyzed. The composite outcome of C. psittaci pneumonia included death during hospitalization, ICU admission, and mechanical ventilation. Univariate and multivariable logistic regression analyses were conducted to determine the significant variables. A ten-fold cross-validation was performed to internally validate the model. The model performance was evaluated using various methods, including receiver operating characteristics (ROC), C-index, sensitivity, specificity, positive/negative predictive value (PPV/NPV), decision curve analysis (DCA), and clinical impact curve analysis (CICA). Results: In total, 83 patients comprised training cohorts and 36 patients comprised validation cohorts. CURB-65 was used to establish predictive Model 1. Multivariate logistic regression analysis identified three independent prognostic factors, including serum albumin, CURB-65, and white blood cells. These factors were employed to construct model 2. Model 2 had acceptable discrimination (AUC of 0.898 and 0.825 for the training and validation sets, respectively) and robust internal validity. The specificity, sensitivity, NPV, and PPV for predicting composite outcome in the nomogram model were 91.7%, 84.5%, 50.0%, and 98.4% in the training sets, and 100.0%, 64.7%, 14.2%, and 100.0% in the validation sets. DCA and CICA showed that the nomogram model was clinically practical. Conclusion: This study constructs a refined nomogram model for predicting the composite outcome in C. psittaci pneumonia patients. This nomogram model enables early and accurate C. psittaci pneumonia patients' evaluation, which may improve clinical outcomes.

2.
Cells ; 12(10)2023 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-37408272

RESUMO

Large-scale use of antimicrobials in agriculture and medicine contributes to antibiotic residues in raw foods, the spread of antimicrobial resistance (AMR) and drug pollution, which seriously threatens human health and imposes significant economic burdens on society, suggesting the need for novel therapeutic options that prevent or control zoonoses. In this study, four probiotics were selected to assess their capability to alleviate pathogen-induced damage. Results showed that a simulated gastrointestinal juice and bile tolerated L. plantarum Lac16 with high lactic acid secretion can significantly inhibit the growth of multiple zoonotic pathogens. Lac16 also significantly inhibited the biofilm formation and mRNA expression of virulence traits (genes related to virulence, toxins, flagella biogenesis and motility, antibiotic resistance, biofilm formation and AI-2 quorum sensing) of enterohemorrhagic E. coli O157:H7 (EHEC). Furthermore, Lac16 and Lac26 significantly protected C. elegans against zoonotic pathogen-induced (EHEC, S. typhimurium, C. perfringens) deaths. Moreover, Lac16 significantly promoted epithelial repair and ameliorated lipopolysaccharide (LPS)-induced intestinal epithelial apoptosis and barrier dysfunction by activating the Wnt/ß-catenin signaling pathway, and markedly reduced LPS-induced inflammatory responses by inhibiting the TLR4/MyD88 signaling pathway. The present results indicate that Lac16 attenuates enterohemorrhagic E. coli infection-induced damage by inhibiting key virulence traits of E. coli, promoting epithelial repair and improving intestinal epithelial barrier function, which may be mediated by the activated Wnt/ß-catenin signaling pathway and the inhibited TLR4/MyD88 signaling pathway of the intestinal epithelium.


Assuntos
Escherichia coli O157 , Lipopolissacarídeos , Animais , Humanos , Virulência/genética , Caenorhabditis elegans , Receptor 4 Toll-Like , Fator 88 de Diferenciação Mieloide , Escherichia coli O157/genética
3.
Behav Brain Res ; 438: 114172, 2023 02 13.
Artigo em Inglês | MEDLINE | ID: mdl-36280009

RESUMO

Anxiety-like behavior and social withdrawal induced by obesity and oxidative stress are significant health concerns in contemporary society. Our previously study found that Bacillus amyloliquefaciens SC06 (SC06) decreased the body weight of high-fat diet (HFD)-fed male mice and protected porcine intestinal epithelial cells against oxidative stress. The present study further investigated the effect of SC06 on HFD-induced obesity, anxiety-like behavior and social withdrawal of male mice and explored its mechanism. Results showed that SC06 significantly decreased HFD-induced obesity as evidenced by the decreased body weight, weight of liver and epididymal fat. Meanwhile, SC06 attenuated the anxiety-like behavior of HFD-fed male mice as illustrated by the more exploration time in both the open arms of elevated plus maze and the central area of open field and the reversed their social withdrawal tested in the three-chamber social choice task. SC06 also reduced reactive oxygen species (ROS) concentration and normalized the mitochondrial morphology in the hippocampus. SC06 reduced the systemic inflammation and increased the expression of intestinal tight junctions (ZO-1 and Claudin1). Furthermore, SC06 also altered the microbial diversity and composition, and decreased Firmicutes to Bacteroidetes ratio of HFD-fed male mice. These findings suggest SC06 attenuate HFD-induced anxiety-like behavior and social withdrawal of male mice by attenuating hippocampal oxidation stress, systemic inflammation, dysbiosis and improving intestinal barrier function.


Assuntos
Bacillus amyloliquefaciens , Disbiose , Camundongos , Animais , Masculino , Suínos , Disbiose/metabolismo , Dieta Hiperlipídica/efeitos adversos , Antioxidantes/farmacologia , Obesidade/metabolismo , Inflamação , Ansiedade , Isolamento Social , Camundongos Endogâmicos C57BL
4.
3 Biotech ; 12(9): 225, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35975024

RESUMO

Bisphenol A (BPA) is a representative example of an endocrine-disrupting chemical. It is one of the most produced chemical substances in the world, but it causes harmful effects in organisms, such that the effective degradation of BPA is critical. The white-rot fungus Phanerochaete sordida YK-624 has been shown to effectively degrade BPA under ligninolytic and non-ligninolytic conditions. However, it is still unclear what kinds of enzymes are involved in BPA degradation. To explore the mechanism of BPA degradation, the present study analysed the functional genes of P. sordida YK-624 using RNA-sequencing (RNA-Seq). Oxidation-reduction process and metabolic pathway were enriched under ligninolytic and non-ligninolytic conditions by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. It is suggested that BPA might be used as a carbon source by P. sordida YK-624. Lignin peroxidase and cytochrome P450 were detected in upregulated differentially expressed genes (DEGs). The lignin-degrading enzyme lignin peroxidase and the intracellular cytochrome P450 system were involved in BPA degradation by P. sordida YK-624, respectively. Furthermore, quantitative real-time PCR (qPCR) was used to validate the reliability of the RNA-Seq results. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03298-w.

5.
Food Funct ; 13(9): 5381-5395, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35470823

RESUMO

Dietary interventions with probiotics have been widely reported to be effective in regulating obesity, and the intestinal microbiota is considered to be an important environmental factor. However, few reports focus on the interactions of microbiota-metabolites-phenotypic variables in ob/ob mice, and they have not been characterized in great detail. In this study, we investigated the effects of Bacillus amyloliquefaciens SC06 on obesity, the intestinal microbiota and the bile acid metabolism of ob/ob mice using biochemical testing, histochemical staining, high-throughput sequencing of the 16S rRNA gene, LC-MS/MS analysis and qRT-PCR. The results showed that SC06 ameliorated the fat mass percentage, hepatic steatosis and liver lipid metabolism disorders and reshaped the gut microbiota and metabolites in male ob/ob mice, specifically deceasing f_S24-7, p_TM7, s_Alistipes massiliensis, f_Rikenellaceae, f_Prevotellaceae, f_Lactobacillaceae, g_Alistipes, g_Flexispira, g_Lactobacillus, g_Odoribacter, g_AF12 and g_Prevotella and increasing f_Bacteroidaceae, g_Bacteroides and f_Desulfovibrionaceae. Meanwhile, SC06 treatment groups had lower ibuprofen and higher glycodeoxycholic acid and 7-dehydrocholesterol. Correlation analysis further clarified the relationships between compositional changes in the microbiota and alterations in the metabolites and phenotypes of ob/ob mice. Moreover, SC06 downregulated bile acid synthesis, export and re-absorption in the liver and increased ileum re-absorption into the blood in ob/ob mice, which may be mediated by the FXR-SHP/FGF15 signaling pathway. These results suggest that Bacillus amyloliquefaciens SC06 can ameliorate obesity in male ob/ob mice by reshaping the intestinal microbial composition, changing metabolites and regulating bile acid metabolism via the FXR signaling pathway.


Assuntos
Bacillus amyloliquefaciens , Microbioma Gastrointestinal , Animais , Ácidos e Sais Biliares/farmacologia , Cromatografia Líquida , Metabolismo dos Lipídeos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Obesidade/tratamento farmacológico , RNA Ribossômico 16S , Espectrometria de Massas em Tandem
6.
Probiotics Antimicrob Proteins ; 12(2): 649-656, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-30891680

RESUMO

Probiotics have always been considered as a supplementary therapy for many diseases especially gut disorders. The absorption and barrier function of the gut play a vital role in the maintenance of body homeostasis. This study was to investigate the protective effects of Bacillus amyloliquefaciens SC06 (Ba) on H2O2-induced oxidative stress on intestinal porcine epithelial cells (IPEC-1) based on the level of gene expression. We demonstrated that Ba was a safe probiotic strain in the first place. Results showed that treatment with H2O2 significantly increased the mRNA expression of absorptive transporters glucose transporter 2 (GLUT2), Ala/Ser/Cys/Thr transporter 1 (ASCT1), and ASCT2 compared with the control group. Meanwhile, oxidative stress induced a significant improvement in the mRNA expression of occludin (OCLN) and caspase-3, and remarkably inhibited the expression of L-type amino acid transporter 1 (LAT1) or B cell lymphoma-2 (Bcl-2), respectively. Pretreatment with Ba dramatically reversed the disturbance induced by oxidative stress on the mRNA expression of ASCT1, ASCT2, and OCLN, which also significantly prevented H2O2-inhibited LAT1 and Bcl-2 mRNA expression. However, Ba failed to exert any significant protective effect on GLUT2 and caspase-3 mRNA expression. We concluded that pretreatment with Ba could alleviate the damage caused by oxidative stress to a certain extent and conferred a protective effect to the intestine.


Assuntos
Bacillus amyloliquefaciens/fisiologia , Células Epiteliais , Intestinos , Estresse Oxidativo , Probióticos , Animais , Linhagem Celular , Células Epiteliais/citologia , Expressão Gênica , Intestinos/citologia , Espécies Reativas de Oxigênio/metabolismo , Suínos
7.
Poult Sci ; 98(10): 5028-5039, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31064013

RESUMO

This study aimed to investigate the effects of Lactobacillus plantarum 16 (Lac16) and Paenibacillus polymyxa 10 (BSC10) on intestinal barrier function, antioxidative capacity, apoptosis, immune response, and biochemical parameters in broilers. A total of 540 one-day-old broiler chicks (Cobb500) were randomly allocated to three groups of 180 birds, and fed either a basal diet or a basal diet supplemented with 108 colony-forming units Lac16 or BSC10 per kilogram feed for 21 D. The results revealed that both Lac16 and BSC10 maintained ileal mucosal morphology, and BSC10 regulated the expression of barrier function-related genes. Birds fed with probiotics decreased malondialdehyde level in jejunal mucosa and serum, and the increased activities of hepatic GSH-Px and jejunal CAT were observed in BSC10 group (P < 0.05). Immunohistochemistry of Bax, Bcl-2 and proliferating cell nuclear antigen and TUNEL-immunofluorescence assay demonstrated that Lac16 and BSC10 exerted beneficial effects on cell apoptosis and proliferation, as indicated by the gene expression of down-regulated Bax and p53 as well as a significant upregulation of Bcl-2 (P < 0.05). In addition, Lac16 and BSC10 significantly increased NO production and iNOS activity in liver and jejunal mucosa, and gene expression of IFN-γ (P < 0.01), IL-6 (P < 0.05), and IL-10 (P < 0.05 and P < 0.01, respectively) in ileum mucosa, whereas markedly decreased the expression of Cox2 (P < 0.05). Furthermore, it was found that Lac16 and BSC10 significantly reduced levels of alkaline phosphatase (P < 0.05 and P < 0.01, respectively) and creatine kinase (P < 0.05). Moreover, BSC10 significantly reduced uric acid (P < 0.05) and low-density lipoprotein levels (P < 0.01). Taken together, Lac16 and BSC10 could improve intestinal and body health status of broilers by increasing intestinal barrier function, anti-oxidative capacity and immunity, and decreasing cell apoptosis with strain-specificity.


Assuntos
Antioxidantes/metabolismo , Galinhas/fisiologia , Imunidade Inata/efeitos dos fármacos , Intestinos/efeitos dos fármacos , Lactobacillus plantarum/química , Paenibacillus polymyxa/química , Probióticos/farmacologia , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Galinhas/imunologia , Dieta/veterinária , Intestinos/fisiologia , Distribuição Aleatória
8.
Food Funct ; 10(12): 7653-7666, 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31742290

RESUMO

Bacillus amyloliquefaciens SC06 (BaSC06), a potential probiotic, plays a positive role in animal growth performance and immune function. The aim of the present study was to investigate the protective effect of BaSC06 against Salmonella infection and its association with macrophage polarization. C57BL/6 mice were fed with or without a BaSC06-containing diet before Salmonella enterica Typhimurium (ST) challenge. Results showed that BaSC06 had a protective effect against ST inoculation and induced both M1 and M2 macrophage polarization in the cecum. An in vitro co-culture model demonstrated that BaSC06 promoted M1 polarization directly, and thus increased the phagocytosis and bactericidal activity against ST. In addition, adoptive transfer of bone marrow-derived macrophages (BMDMs) stimulated by BaSC06 significantly decreased the counts of ST in the spleen. Furthermore, 16S rRNA-based analysis of cecal content showed that BaSC06 significantly increased the proportion of Verrucomicrobia and decreased Bacterodetes. Transplantation of the fecal microbiota from BaSC06-treated animals promoted M2 macrophage polarization in the cecum and significantly relieved inflammation caused by ST. In conclusion, BaSC06 polarized macrophages to the M1 type directly resulting in excellent bactericidal activity. Meanwhile, the microbiota modified by BaSC06 can induce M2 polarization which ameliorates the inflammation caused by ST.


Assuntos
Bacillus amyloliquefaciens/fisiologia , Macrófagos/imunologia , Microbiota , Probióticos/administração & dosagem , Infecções por Salmonella/prevenção & controle , Salmonella typhimurium/efeitos dos fármacos , Animais , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Polaridade Celular/efeitos dos fármacos , Humanos , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Masculino , Camundongos Endogâmicos C57BL , Microbiota/efeitos dos fármacos , Fagocitose/efeitos dos fármacos , Infecções por Salmonella/imunologia , Infecções por Salmonella/microbiologia , Salmonella typhimurium/fisiologia
9.
Front Microbiol ; 10: 52, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30761104

RESUMO

Gut microbiota has indispensable roles in nutrient digestion and energy harvesting, especially in processing the indigestible components of dietary polysaccharides. Searching for the microbial taxa and functional capacity of the gut microbiome associated with feed efficiency (FE) can provide important knowledge to increase profitability and sustainability of the swine industry. In the current study, we performed a comparative analysis of the fecal microbiota in 50 commercial Duroc × (Landrace × Yorkshire) (DLY) pigs with polarizing FE using 16S rRNA gene sequencing and shotgun metagenomic sequencing. There was a different microbial community structure in the fecal microbiota of pigs with different FE. Random forest analysis identified 24 operational taxonomic units (OTUs) as potential biomarkers to improve swine FE. Multiple comparison analysis detected 8 OTUs with a significant difference or tendency toward a difference between high- and low-FE pigs (P < 0.01, q < 0.1). The high-FE pigs had a greater abundance of OTUs that were from the Lachnospiraceae and Prevotellaceae families and the Escherichia-Shigella and Streptococcus genera than low-FE pigs. A sub-species Streptococcus gallolyticus subsp. gallolyticus could be an important candidate for improving FE. The functional capacity analysis found 18 KEGG pathways and CAZy EC activities that were different between high- and low-FE pigs. The fecal microbiota in high FE pigs have greater functional capacity to degrade dietary cellulose, polysaccharides, and protein and may have a greater abundance of microbes that can promote intestinal health. These results provided insights for improving porcine FE through modulating the gut microbiome.

10.
Front Immunol ; 9: 2321, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30459751

RESUMO

Glycyrrhizin (GL), a triterpenoid glycoside, serves important functions in various biological activities, including antiviral and antitumor immune responses. However, the anti-inflammatory effects of GL on Salmonella enterica serovar Typhimurium (ST)-induced injury in mice and the mechanisms underlying the protection of GL are poorly understood. Here, we investigated the effects of GL on host immune responses against ST infection in mice. A phenotypic analysis using hematoxylin and eosin (H&E) staining and transmission electron microscopy showed that GL relieved ST-induced weight loss and intestinal mucosal injury. A colonization assay showed that GL significantly reduced ST colonization in the ileum and colon and translocation to the liver and spleen. An antibacterial activity assay and real-time PCR revealed that GL had no direct inhibitory impact on ST growth or virulence gene expression. ELISA showed that GL pretreatment significantly decreased proinflammatory cytokine (IFN-γ, TNF-α, IL-6) secretion and increased anti-inflammatory cytokine (IL-10) secretion in the ileum, colon and serum of ST-infected mice. Moreover, flora analysis showed that GL reduced Akkermansia, Sutterella, Prevotella and Coprococcus but enriched Parabacteroides and Anaerotruncus in the cecum of ST-infected mice. These results suggest that GL promotes the secretion of immune factors and modulates intestinal flora to prevent further ST infection. We also analyzed the effect of GL on immunocytes and found that GL promoted the phenotypic and functional maturation of murine bone marrow-derived dendritic cells (BMDCs). Flow cytometry and western blotting demonstrated that NF-κB, ERK, and p38 MAPK were required for GL-induced BMDC maturation. The above findings indicate that GL attenuates ST infection by modulating immune function and intestinal flora. This study enriches our current knowledge of GL-mediated immunological function and provides a new perspective on the prevention of Salmonella infection in animals and humans.


Assuntos
Ácido Glicirrízico/farmacologia , Interações Hospedeiro-Patógeno/efeitos dos fármacos , Interações Hospedeiro-Patógeno/imunologia , Infecções por Salmonella/imunologia , Infecções por Salmonella/microbiologia , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/fisiologia , Animais , Apresentação de Antígeno , Citocinas/biossíntese , Citocinas/sangue , Citotoxicidade Imunológica , Células Dendríticas/imunologia , Células Dendríticas/metabolismo , Modelos Animais de Doenças , Feminino , Microbioma Gastrointestinal , Regulação Bacteriana da Expressão Gênica , Mediadores da Inflamação/sangue , Mediadores da Inflamação/metabolismo , Mucosa Intestinal/imunologia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Mucosa Intestinal/patologia , Camundongos , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/metabolismo , Infecções por Salmonella/metabolismo , Infecções por Salmonella/patologia , Salmonella typhimurium/patogenicidade , Transdução de Sinais , Virulência/genética
11.
Orthopedics ; 32(12): 925, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19968232

RESUMO

Osseous hemangiomas are mainly seen in the skull or spine and rarely occur in other bones. They are small lesions usually localized in the metaphyseal and diaphyseal regions. This article describes a rare case of a giant intraosseous hemangioma occupying nearly the whole humerus for 28 years. Initially, the patient, a 36-year-old woman, had been misdiagnosed with fibrous hyperplasia of the left humerus. We intended to curette the lesions and transplant fibula to fill the bone defect, but the initial surgical attempt could not be completed due to massive intraoperative bleeding. In the second surgery, she was treated by blocking the blood flow of the subclavian artery temporarily through a balloon catheter, curetting lesions, and filling the defect of bone with bone cement and K-wires. At 12-month follow-up, there was no evidence of local recurrence of hemangioma or loosening of bone cement. However, longer-term follow-up is needed to confirm the success of the surgery. Although hemangiomas of long bone are rare, they should be considered in the differential diagnosis of bone tumors.


Assuntos
Neoplasias Ósseas/diagnóstico por imagem , Neoplasias Ósseas/cirurgia , Hemangioma/diagnóstico por imagem , Hemangioma/cirurgia , Úmero/diagnóstico por imagem , Úmero/cirurgia , Adulto , Feminino , Humanos , Masculino , Radiografia , Resultado do Tratamento
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