MRI-based radiomics model for preoperative prediction of 5-year survival in patients with hepatocellular carcinoma.

BACKGROUND: Recurrence is the major cause of mortality in patients with resected HCC. However, without a standard approach to evaluate prognosis, it is difficult to select candidates for additional therapy. METHODS: A total of 201 patients with HCC who were followed up for at least 5 years after curative hepatectomy were enrolled in this retrospective, multicentre study. A total of 3144 radiomics features were extracted from preoperative MRI. The random forest method was used for radiomics signature building, and five-fold cross-validation was applied. A radiomics model incorporating the radiomics signature and clinical risk factors was developed. RESULTS: Patients were divided into survivor (n = 97) and non-survivor (n = 104) groups based on the 5-year survival after surgery. The 30 most survival-related radiomics features were selected for the radiomics signature. Preoperative AFP and AST were integrated into the model as independent clinical risk factors. The model demonstrated good calibration and satisfactory discrimination, with a mean AUC of 0.9804 and 0.7578 in the training and validation sets, respectively. CONCLUSIONS: This radiomics model is a valid method to predict 5-year survival in patients with HCC and may be used to identify patients for clinical trials of perioperative therapies and for additional surveillance.

Effects of the toxic metal zinc on the growth, development, and reproduction of the wolf spider Pardosa laura through its food Drosophila melanogaster.

In recent years, toxic metal pollution has become a serious problem, and its influence on human society and the ecological environment has become a hot topic for people. Toxic metal pollution is ubiquitous in the environment, and it can affect the growth, development, and reproduction of organisms through food chain transmission. In this study, we used ZnSO4 and set three different Zn concentrations [0 mg/mL (CK), 0.25 mg/mL, and 0.50 mg/mL] to study the enrichment of toxic zinc metal in the wolf spider Pardosa laura through the food chain (medium-Drosophila melanogaster-P. laura) and the effects on the growth, development, and reproduction of D. melanogaster and P. laura. The results of the study showed that the Zn content in D. melanogaster and P. laura significantly increased with the increase of Zn concentration in the culture medium, reflecting the obvious food chain enrichment phenomenon. The inhibitory effect of zinc treatment on the growth, development, and reproduction of D. melanogaster was significant and more pronounced with increasing Zn concentration. The transfer of zinc through the food chain also had a significant inhibitory effect on the growth, development, and reproduction of P. laura. The developmental duration and preoviposition period were significantly prolonged. Moreover, the survival rate, body size, and egg laying amount were significantly reduced. This study will enrich and improve the research on the effects of toxic Zn metal pollution on spiders and provide a theoretical basis for monitoring and evaluating the environmental quality using farmland spiders.

Simple and selective sensing of cysteine using gold nanoparticles modified by ruthenium(II) complexes.

A simple and selective luminescence sensing method for the cysteine detection was developed based on gold nanoparticles modified by the new ruthenium(II) complexes. The intense emission of the modified ruthenium(II) complexes was quenched efficiently by gold nanoparticles due to the energy and charge transfer between the ruthenium(II) fluorophores and gold nanoparticles. Upon addition of cysteine, the emission of the ruthenium(II) complexes was enhanced significantly by the release of the ruthenium(ll) complexes from the surface of the gold nanoparticles. Therefore, cysteine could be detected by this gold nanoparticles-ruthenium(II) complexes based probes. The synthesis of gold nanoparticles and the modification based fluorophores probed could be accomplished successfully within one step, which simplified the preparation of luminescence sensors. Moreover, since metal-to-ligand charge transfer transition (3MLCT) emission band of the ruthenium(II) complexes was in the visible region, this approach was available for biomolecular sensing applications, and its relatively long life time made it suitable for the biological process studies.

Selective recognition of homocysteine and cysteine based on new ruthenium(II) complexes.

A series of the new ruthenium(II) complexes with different number of aldehyde groups have been synthesized and characterized for the simple and selective sensing of homocysteine (Hcy) and cysteine (Cys). The reaction of these ruthenium(II) complexes with Hcy and Cys afforded thiazinane or thiazolidine derivatives which resulted in the obvious changes in the UV-visible spectra and strong enhancement of the luminescence intensity of the system. The luminescence enhancement of [Ru(dmb)(2)(L2)](2+) (dmb: 4,4'-dimethyl-2,2'-bipyridine) showed a good linearity in the concentration of 4.2-350 µM and 6-385 µM with the detection limits of 0.3 µM and 1 µM for Hcy and Cys, respectively. The absorption and emission bands from metal-to-ligand charge transfer transition in the visible region and the large Stokes shift of the ruthenium(II) complex chromophore made it suitable for biological applications.