RESUMO
Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair. But the rapid biodegradation of the materials may interrupt neural tissue reconstruction in vivo. To improve the stability of the materials, the materials produced by NECM and chitosan hydrogels were crosslinked by genipine, glutaraldehyde or ultraviolet ray. Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray were evaluated. The scaffold crosslinked by genipin possessed a porous structure, and the porosity ratio was 89.07 + 4.90%, the average diameter of pore was 85.32 + 5.34 µm. The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity. The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups. Cell cytotoxicity showed that the cytotoxicity of scaffold crosslinked by genipin was lower than that crosslinked by glutaraldehyde. The histocompatibility of scaffold crosslinked by genipin was also better than glutaraldehyde group. More cells grew well in the scaffold crosslinked by genipin when co-cultured with L929 cells. The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.