Unclonable Encryption-Verification Strategy Based on Bilayer Shape Memory Photonic Crystals.

The ability to reversibly exhibit structural color patterns has positioned photonic crystals (PCs) at the forefront of anti-counterfeiting. However, the security offered by the mere reversible display is susceptible to illicit alteration and disclosure. Herein, inspired by the electronic message captcha, bilayer photonic crystal (BPC) systems with integrated decryption and verification modules, are realized by combining inverse opal (IO) and double inverse opal (DIO) with polyacrylate polymers. When the informationized BPC is immersed in ethanol or water, the DIO layer displayed encrypted information due to the solvent-induced ordered rearrangement of polystyrene (PS) microspheres. The verification step is established based on the different structural colors of the IO layer pattern, which result from the deformation or recovery of the macroporous skeleton induced by solvent evaporation. Moreover, through the evaporation-induced random self-assembly of PS@SiO2 and SiO2 microspheres, unclonable structurally colored identifying codes are created in the IO layer, ensuring the uniqueness upon the verification. The decrypted code in the DIO layer is valid only when the IO layer displays the pattern with the predetermined structural color; otherwise, it is a pseudo-code. This structural color-based "decryption-verification" approach offers innovative anti-counterfeiting applications in nanophotonics.

2D Information Security System Based on Polyurethane Inverse Photonic Glass Structure.

Information security has become a major global problem in recent years. Thus, people continue to exert much effort in developing new information security technologies based on encryption and storage. In this study, a 2D information security technology based on polyurethane optical devices with inverse photonic glass structure (PU-IPG) is introduced. Based on 1) the swelling and plasticizing effects of various solvents on PU-IPG and 2) the capillary force that can produce geometric deformation on micro/nanostructures when solvents evaporate, a 2D information security system with two modules of decryption (structural color information display) and anticounterfeiting (structural color transformation) is successfully constructed. The spraying method adopted can be simple and fast and can provide a large area to build photonic glass templates, which greatly improves the capacity and category of information in the encryption system. The prepared PU-IPG optical devices can produce large-area multicolor output capability of information. These devices also have excellent mechanical properties, strong cycle stability, environmental friendliness, and low price. Therefore, the preparation strategy has great reference value and application prospects in the field of information security.

Infrared Stealth Coating with Tunable Structural Color Based on ZnO Spheres.

It is important to develop low infrared (IR) emissive coating with tunable structure color to improve the infrared-visible stealth performance of military equipment. In this work, uniform ZnO spheres are used as building units to construct photonic structures with both bright adjustable structure color and low IR emissivity due to the relatively high refractive index and low IR emissivity of ZnO. The vivid tunable structural colors are provided by the photonic bandgap of ZnO photonic crystals (PCs) or the quasi-bandgap of amorphous photonic crystals (APCs), respectively. Both ZnO PCs and APCs exhibited low IR emissivity in 3-5 µm. The IR emissivity of 255 nm ZnO PC is 0.483 and the IR emissivity of 255 nm ZnO APC is 0.492 at 25 °C. With the increase of temperature, the IR emissivity of further decreased to 0.295 and 0.312 at 300 °C. These structures can be applied to a variety of surfaces, and all these structures have good thermal and light stability as well. This work may open a simple and effective way to fabricate materials with good infrared-visible stealth performance, expanding the application of ZnO PCs and APCs coatings in the camouflage area.

Visual Recognition of Volatile Organic Compounds by Photonic Nose Integrated with Multiple Metal-Organic Frameworks.

The photonic nose inspired by the olfactory system is an integrated detection platform constructed by multiple sensing units as channels. However, in the detection of volatile organic compounds (VOCs), the sensing results that cannot be directly readable and the poor ability to distinguish analytes with similar chemical properties are the main challenges faced by this sensor. Here, 8 metal-organic frameworks (MOF)-based photonic crystals are used as the basic sensing units to construct a photonic nose detection platform. The microscopic adsorption of VOCs by MOFs enables the photonic crystals (PCs) to produce macroscopic structural color output, and further makes the photonic nose have specific color fingerprints for different VOCs, the response time of all PCs to VOCs can be within 1 s. Through the color fingerprint, the visual identification of VOCs produced by 5 common solvent vapors is realized, and 9 VOCs with similar chemical properties are further distinguished. In addition, the application potential of the photonic nose in the actual environment is verified by identifying different contents of benzene in the paint. It is envisaged that the MOF-based photonic nose has great reference value for the development of intelligent and multi-component synergistic functional gas sensors.

Residue-Free and Recyclable Starch-Based Flocculants for Dye Wastewater Flocculation.

Flocculants are crucial agents in wastewater treatment because they can remove oppositely charged impurities effectively and swiftly. However, flocculation also inevitably causes secondary contamination due to the residual properties, nonreusability, and nondegradability of traditional flocculant molecules. Herein, an ecofriendly starch-based flocculant, i.e., 2,4-bis(dimethylamino)-[1,3,5]-triazine-6-starch, was synthesized via a preactivation-etherification strategy. The large molecular weight property of the flocculant produced by this method enhances the intermolecular hydrophobic association, achieving complete phase separation of all flocculant molecules from water and residue-free flocculation for the first time. Importantly, a large molecular weight tertiary amine starch-based flocculant (LMTS) exhibits a remarkable flocculation capacity of over 1800 mg·g-1 for dye wastewater, which is significantly higher than that of traditional polyacrylamide and polyaluminum chloride flocculants. Furthermore, the LMTS flocculant could be recycled by pH adjustment, and its structural stability ensured sustained reusability. This high-performance residue-free biomass-based flocculant offers a green advance for wastewater treatment.

Bioinspired Colorimetric Double Inverse Opal Photonic Crystal Indicators for Ethanol Concentration Sensing in Fermentation Engineering.

Photonic crystal-based ethanol concentration indicators with rapid response and brilliant structural color output definitely take a place in colorimetric sensors. Here, based on the H-bond-regulated swelling of acrylate shape memory polymers (SMPs) and the solvent-induced structural color change of the double inverse opal photonic crystals (DIOPCs), new-type photonic crystals (PCs) colorimetric indicators were constructed, exhibiting a span of maximum reflection wavelength (λmax) up to ∼166 nm in response to alcohols with concentrations from 0 to 100 vol %. DIOPC indicators (DIOPCIs) show a rapid response to alcohols (<1.5 s) and output different structural colors (covering from blue to red). The colorimetric sensing mechanism includes the solvent-triggered recovery of the inverse opal skeleton, the cosolvency effect and H-bonds induced swelling/shrinkage of the polymer, the phase separation between polystyrene (PS) microsphere and polymer skeleton, and the light diffraction of DIOPCs. While ensuring a larger λmax span by regulating the H-bond interactions in polymer chains through acrylamide (AAm), AAm-modified DIOPCIs are sensitive to some specific ethanol concentrations. The real-time sensing of ethanol concentration during fermentation verified the practicability of DIOPCIs, thus establishing a visual model between structural color and corresponding fermentation kinetics. We envisage that the DIOPCIs will contribute to the intelligentization of the alcoholic fermentation and distillation industry.

microRNA-15a-5p suppresses hypoxia-induced tumor growth and chemoresistance in bladder cancer by binding to eIF5A2.

In various malignant tumors (including bladder cancer) poor prognosis is associated with hypoxia and therapeutic resistance. Evidence indicates that in bladder cancer, microRNAs (miRNAs) have vital functions in acquired drug resistance. However, the involvement of miRNAs in hypoxia-mediated bladder cancer doxorubicin (Dox) resistance is unknown. Herein, we showed that hypoxia and Dox treatment downregulated miR-15a-5p expression. Using UM-UC-3 and J82 bladder cancer cell lines and in vivo mouse models of bladder cancer, we confirmed that miR-15a-5p arrests tumor cell growth and Dox resistance in vitro and in vivo. Furthermore, we determined the interaction between miR-15a-5p and eukaryotic translation initiation factor 5A-2 (eIF5A2) using dual luciferase reporters and quantitative real-time reverse transcription polymerase chain reaction assays. We also showed that a miR-15a-5p agomir repressed EIF5A2 expression in bladder cancer cells, thereby inhibiting the epithelial-mesenchymal transition (EMT) induced by Dox or hypoxia. Moreover, ectopic expression of miR-15a-5p abrogated eIF5A2-mediated Dox resistance in bladder cancer cells. Collectively, these data indicated that hypoxia promotes tumor growth and chemoresistance through the HIF-1α/miR-15a-5p/eIFTA2/EMT pathway. This new finding not only has implications for improving our understanding of the Dox resistance process during bladder cancer progression but also indicates that the miR-15a-5p agomir is a promising tool to prevent Dox resistance in patients with bladder cancer.

Correlation Between Persistent HPV Infection and Vaginal Microecological Imbalance After Treatment of Cervical High-Grade Squamous Intraepithelial Lesion.

Objective: To analyze the association between persistent human papillomavirus (HPV) infection and vaginal microecological imbalance after surgical treatment of cervical high-grade squamous intraepithelial lesion (HSIL). Methods: This is a retrospective study, 180 cervical HSIL patients admitted to our hospital from May 2019 to May 2021 were selected, of these, 84 were treated with loop electrosurgical excision procedure (LEEP) and 96 with cold knife conization (CKC). Patients were followed up for HPV infection 1 year after surgery. There is a division into a persistent infection group (positive group) and a negative group based on the presence or absence of HPV, the detection technique was PCR amplification. The two groups were compared regarding preoperative HPV infection, vaginal micro-ecological indicators 1 year after surgery, and the correlation between persistent HPV infection and vaginal microecological imbalance. Results: At 1 year after surgery, among 180 cervical HSIL patients, 64 (35.56%) were persistently infected with HPV, with an age of (40.20 ± 4.85) years, including 36 (56.25%) with cervical intraepithelial neoplasia (CIN) grade II, 28 (43.75%) with cervical intraepithelial neoplasia (CIN) grade III, 116 (64.44%) with HPV negative, with an age of (40.22 ± 5.15) years, including 67 (57.76%) with CIN grade II and 49 (42.24%) with CIN grade III, the differences in age and CIN classification between the two groups were not statistically significant (P > .05). Preoperatively, 53 people (82.81%) with HPV viral load >100 RLU/CO in the HPV persistent infection group and 76 people (65.52%) with HPV viral load >100 RLU/CO in the HPV negative group, with statistically significant differences between the two groups (P < .05); The difference in HPV virus typing and HPV infection type between the two groups was not statistically significant (P > .05). At 1 year after surgery, the composition ratio of flora density class IV and flora diversity class IV were significantly higher in the HPV persistent infection group than in the HPV negative group, and the dominant bacteria were mainly gram-positive large bacillus, accounting for 83.33%, the difference between the two groups was statistically significant (P < .05); The differences in Nugent scores and pH values between the two groups were not statistically significant (P > .05). Logistic regression analysis showed that flora density, flora diversity, and dominant bacteria were all independent risk factors for persistent HPV infection after treatment in patients with HSIL (P < .05). Conclusion: After treatment of HSIL patients, clinical attention should be paid to monitoring of HPV infection but also to the changes in vaginal microecology, as timely correction of vaginal microecology can facilitate HPV regression and improve the patient's prognosis.

Normalizing Tumor Blood Vessels to Improve Chemotherapy and Inhibit Breast Cancer Metastasis by Multifunctional Nanoparticles.

The abnormal tumor blood vessels with high leakage can promote tumor cells to infiltrate into the systemic circulation and increase the risk of tumor metastasis. In addition, chemotherapy may destroy tumor blood vessels and further aggravate metastasis. Normalizing tumor blood vessels can reduce vascular leakage and increase vascular integrity. The simultaneous administration of vascular normalization drugs and chemotherapy drugs may resist the blood vessels' destruction of chemotherapy. Here, multifunctional nanoparticles (CCM@LMSN/DOX&St), which combined chemotherapy with tumor blood vessel normalization, were prepared for the treatment of breast cancer. The results showed that CCM@LMSN/DOX&St-loaded sunitinib (St) promoted the expression of junction proteins Claudin-4 and VE-cadherin of endothelial cells, reversed the destruction of DOX to the endothelial cell layer, protected the integrity of the endothelial cell layer, and inhibited the migration of 4T1 tumor cells across the endothelial cell layer. In vivo experiments showed that CCM@LMSN/DOX&St effectively inhibited tumor growth in situ; what is exciting was that it also inhibited distal metastasis of breast cancer. CCM@LMSN/DOX&St encapsulated with St can normalize tumor blood vessels, reverse the damage of DOX to tumor blood vessels, increase the integrity of blood vessels, and prevent tumor cell invasion into blood vessels, which can inhibit breast cancer spontaneous metastasis and reduce chemotherapy-induced metastasis. This drug delivery platform effectively inhibited the progression of tumors and provided a promising solution for effective tumor treatment.

General Method to Synthesize Highly Stable Nanoclusters via Pickering-Stabilized Microemulsions.

The ability to not only control but also maintain the well-defined size of nanoclusters is key to a scientific understanding as well as their practical application. Here, we report a synthetic protocol to prepare and stabilize nanoclusters of different metals and even metal salts. The approach builds on a Pickering stabilization effect inside a microemulsion system. We prove that the emulsion interface plays a critical role in the formation of nanoclusters, which are encapsulated in situ into a silica matrix. The resulting nanocapsule is characterized by a central cavity and a porous shell composed of a matrix of both silica and nanoclusters. This structure endows the nanoclusters simultaneously with high thermal stability, good biocompatibility, and excellent photostability, making them well suited for fundamental studies and practical applications ranging from materials chemistry, catalysis, and optics to bioimaging.

GSH-Responsive Polymeric Micelles for Remodeling the Tumor Microenvironment to Improve Chemotherapy and Inhibit Metastasis in Breast Cancer.

The tumor microenvironment (TME) of breast cancer is hypoxic, which can promote tumor progression, including invasion and metastasis, and limit the efficacy of anti-tumor treatment. Nitric oxide (NO) can dilate blood vessels, effectively alleviate hypoxia, and regulate the TME, which has the potential to improve the anti-tumor therapeutic efficacy. Here, chitosan (CO) and octadecylamine (ODA) were linked by the disulfide bond, and the LinTT1 peptide was linked onto CO-SS-ODA for targeting tumor cells and endothelial cells in tumors. The NO donor S-nitroso-N-acetylpenicillamine (SNAP) was connected to CO. Doxorubicin (DOX) was encapsulated, and GSH hierarchically responsive polymer micelles (TSCO-SS-ODA/DOX) were constructed for the treatment of breast cancer. The micelles had differently responsive drug release in different GSH concentrations. In endothelial cells, the micelles rapidly responded to release NO. In tumor cells, the disulfide bond rapidly broke and released DOX to effectively kill tumor cells. The disulfide bond was not sensitive to GSH concentration in endothelial cells, which had less release of DOX. The killing effect of the micelles to endothelial cells was much lower than that to tumor cells. The cell selective drug release of the drug delivery systems enabled safe and effective treatment of drugs. TSCO-SS-ODA/DOX, which had the excellent ability to target tumors, can alleviate tumor hypoxia, decrease the infiltration of M2 macrophages in tumors, increase the infiltration of M1 macrophages in tumors, and remodel the TME. Notably, TSCO-SS-ODA/DOX can significantly inhibit the growth of the primary tumor and effectively inhibit tumor metastasis. The drug delivery system provided a potential solution for effectively treating breast cancer.

Bioinspired Polypeptide Photonic Films with Tunable Structural Color.

We report a new synthetic strategy of combining N-carboxyanhydride (NCA) chemistry and photonic crystals for the fabrication of polypeptide structural color films. Driven by surface-initiated ring-opening polymerization, the di-NCA derivative of l-cystine (Cys) is introduced to replicate the functionalized colloidal crystal templates and construct freestanding P(Cys) films with tunable structural color. Furthermore, the feasibility of preparing patterned polypeptide photonic films is demonstrated via template microfabrication. Because of the incorporation of l-glutamate (Glu) components, the P(Cys-co-Glu) co-polypeptide films are endowed with a visual color responsiveness toward pH changes. Additionally, the polypeptide photonic films show on-demand degradability. Given the large family of amino acid building blocks, this powerful and versatile approach paves the way for chemical derivatization of multifunctional peptide-based optical platforms.

Dimethylaminoethyl Methacrylate/Diethylene Glycol Dimethacrylate Grafted onto Folate-Esterified Bagasse Xylan/Andrographolide Composite Nanoderivative: Synthesis, Molecular Docking and Biological Activity.

As a biocompatible biomaterial, bagasse xylan (BX) has been widely used in the biomedical field. The low biological activity of andrographolide (AD) restricts its development, so AD with certain anticancer activity is introduced. We use chemical modification methods such as grafting and esterification to improve the biological activity and make a novel anticancer nanomaterial. On the basis of the esterification of a mixture of BX and AD with folic acid (FA), a novel anticancer nanoderivative of bagasse xylan/andrographolide folate-g-dimethylaminoethyl methacrylate (DMAEMA)/diethylene glycol dimethacrylate (DEGDMA) nanoparticles (FA-BX/AD-g-DMAEMA/DEGDMA NPs) was synthesized by introducing DMAEMA and DEGDMA monomers through a graft copolymerization and nanoprecipitation method. The effects of reaction temperature, reaction time, the initiator concentration and the mass ratio of FA-BX/AD to mixed monomers on the grafting rate (GR) were investigated. The structure of the obtained product was characterized by FTIR, SEM, XRD and DTG. Further, molecular docking and MTT assays were performed to understand the possible docking sites with the target proteins and the anticancer activity of the product. The results showed that the GR of the obtained product was 79% under the conditions of the initiator concentration 55 mmol/L, m (FA-BX/AD):m (mixed monomer) = 1:2, reaction temperature 50 °C and reaction time 5 h. The inhibition rate of FA-BX/AD-g-DMAEMA/DEGDMA NPs on human lung cancer cells (NCI-H460) can reach 39.77 ± 5.62%, which is about 7.6 times higher than that of BX. Therefore, this material may have potential applications in the development of anticancer drug or carriers and functional materials.

Enhanced removal of ibuprofen by heterogeneous photo-Fenton-like process over sludge-based Fe3O4-MnO2 catalysts.

Heterogeneous photo-Fenton-like catalysts with low cost, little hazard, high effectiveness and facile separation from aqueous solution were highly desirable. In this study, sludge-based catalysts combining nano Fe3O4-MnO2 and sludge activated carbon were successfully synthesized by high-temperature calcination method and then characterized. These synthetic materials were applied to remove ibuprofen in the heterogeneous photo-Fenton process. The preparation conditions of sludge-based catalysts optimized by orthogonal experiments were 2.0 M of ZnCl2, a temperature of 500 °C, a pyrolysis time of 60 min, and a sludge ratio: Fe3O4-MnO2 of 25:2. In batch experiments, the optimal experimental conditions were determined as catalyst dosage of 0.4 g·L-1, hydrogen peroxide concentration of 3.0 mL·L-1, pH value of 3.3, and contact time of 2.5 h. The degradation rate sludge/Fe3O4-MnO2 catalyst to ibuprofen is up to 95%. The removal process of ibuprofen fitted the pseudo-second-order kinetic model, and the photocatalytic degradation process was the main factor controlling the reaction rate. The catalytic mechanism was proposed according to the Fourier transform infrared analysis and mass spectrometry product analysis; it was mainly attributed to the interaction between hydroxyl groups and benzene rings.

Function of Astrocytes in Neuroprotection and Repair after Ischemic Stroke.

BACKGROUND: Astrocytes are the most numerous cell types within the central nervous system, and many efforts have been put into determining the exact role of astrocytes in neuroprotection and repair after ischemic stroke. Although numerous studies have been done in recent years, there is still no thorough understanding of the exact function of astrocytes in the whole course of the stroke. SUMMARY: According to the recent literature, there are many structures and factors that play important roles in the process of ischemic stroke, among which blood-brain barrier, various growth factors, gap junctions, AQP4, and glial scars have been studied most comprehensively, and all these factors are closely related to astrocytes. The role of astrocytes in ischemic stroke, therefore, can be analyzed more comprehensively. Key Message: The present review mainly summarized the current knowledge about astrocytes and their potential roles after ischemic stroke.

Thermal Responsive Photonic Crystal Achieved through the Control of Light Path Guided by Phase Transition.

Responsive photonic crystal is widely considered in the field of anti-counterfeiting and information encryption because of their structural color changes caused by external stimulation. However, the response signal is usually achieved by adjusting the periodic lattice constant based on Bragg's law with volume changes. Thus, it is a great challenge to achieve the response of photonic crystals by non-array parameter control. Herein, novel thermal responsive photonic crystal (TRPC) with low angle dependent structural color is fabricated by introducing poly(ethylene glycol) into the structure of low angle dependent SnO2 inverse opal. The response is achieved through the control of light path guided by phase transition and the significant volume change caused by the change of traditional array parameters can be effectively avoided. Meanwhile, the low angle dependent structural color of TRPC can effectively reduce the interference of observation angle change to response signal caused by external thermal stimulation. Patterned responsive photonic crystals with temperature gradient response are easily obtained by combining confinement self-assembly and direct template method, and the patterns can be presented and hidden by the control of light path, showing great potential in anti-counterfeiting and information encryption fields.

Inorganic Photonic Microspheres with Localized Concentric Ordering for Deep Pattern Encoding and Triple Sensory Microsensor.

Photonic microspheres offer building units with unique topological structures and specific optical functions for diverse applications. Here, a new class of inorganic photonic microspheres with superior robustness, optical and electrical properties is reported by introducing a unique localized concentric ordering architecture and chemical interaction, which further serve as building blocks for deep pattern encoding and multiple sensory optoelectronic devices. Benefiting from localized concentric ordering architecture, the resultant photonic microspheres demonstrate orientation- and angle-independent structural colors. Notably, the formation of well-combined lamellae inorganic layers by chemical interaction grants the microspheres superior mechanical robustness, excellent solvent resistance, thermal stability, and multiple optoelectronic properties simultaneously, rarely seen in previous reports. Owing to these merits, such microspheres are used to construct diverse encoded photonic patterns for anti-counterfeiting applications. Interestingly, cross-communication among neighboring microspheres creates complex photonic sub-patterns, which provide "fingerprint information" with deep encryption security. Moreover, a single photonic microsphere-based optoelectronic microsensor is demonstrated for the first time, which achieves appealing application for real-time health monitoring and safety warning toward triple environmental stimuli. This work not only provides a new kind of robust, multifunctional photonic material, but also opens a new avenue for their uses as complexed pattern encoding and multi-parametric sensing platforms.

MiR-223 regulates autophagy associated with cisplatin resistance by targeting FBXW7 in human non-small cell lung cancer.

BACKGROUND: Cisplatin is widely used as a first-line treatment for non-small cell lung cancer (NSCLC), but chemoresistance remains a major clinical obstacle for efficient use. As a microRNA, miR-223 was reported to promote the doxorubicin resistance of NSCLC. However, whether miR-223 is also involved in cisplatin resistance of NSCLC and the mechanism miR-223 involved in drug resistance is unclear. Accumulated evidence has shown that abnormal autophagy is associated with tumor chemoresistance. The study aimed to study the role of miR-223 on cisplatin sensitivity in NSCLC and uncover the potential mechanisms. METHODS: NSCLC cells transfected with mimic or inhibitor for miR-223 was assayed for chemoresistance in vitro. MiR-223 expression was assessed by quantitative real-time PCR (qRT-PCR). Western blot were used to study the expression level of F-box/WD repeat-containing protein 7 (FBXW7) and autophagy-related protein. The effect of miR-223 on cisplatin sensitivity was examined by using CCK-8, EdU assays and Autophagic flux assay. Luciferase assays, EdU assays and small interfering RNA were performed to identify the targets of miR-223 and the mechanism by which it promotes treatment resistance. Xenograft models were established to investigate the effect of mir-223 on cisplatin sensitivity. RESULTS: In the present study, we found that the level of miR-223 was significantly positively correlated with cisplatin resistance. MiR-223 overexpression made NSCLC cells resistant to cisplatin treatment. We further found that autophagy mediated miR-223-mediated cisplatin resistance in NSCLC cells. Further mechanistic research demonstrated that miR-223 directly targeted FBXW7. The overexpression of miR-223 could inhibit the level of FBXW7 protein expression, thus promoting autophagy and making NSCLC cells resistant to cisplatin. Finally, we confirmed the increased effect of cisplatin sensitivity by miR-223 Antagomir in xenograft models of NSCLC. CONCLUSIONS: Our results demonstrate that miR-223 could enhance autophagy by targeting FBXW7 in NSCLC cells. Inhibition of autophagy by miR-223 knockdown provides a novel treatment strategy to alleviate cisplatin resistance in NSCLC.

Bilayer Heterostructure Photonic Crystal Composed of Hollow Silica and Silica Sphere Arrays for Information Encryption.

Utilizing photonic crystals to fabricate information encryption materials has attracted widespread interest due to their tunable optical properties and responsiveness to external stimuli. In most of the previously reported systems, the information is hidden at a specific angle and the angle-dependent invisibility is a limitation. Meanwhile, poor structural stability is still a key issue that needs to be solved for potential applications. In this paper, a bilayer heterostructure photonic crystal containing ordered hollow silica inverse opal arrays, amorphous silica opal arrays, and poly(vinyl alcohol) (adhesive) is successfully constructed. It makes the information highly invisible at any angle and also achieves information encryption. With this strategy, the information can be hidden by the noniridescent structural color derived from the strong scattering effect of light from the top layer of amorphous silica sphere arrays. After wiping with ethanol or a refractive-index-matching solvent, the scattering effect vanishes and the amorphous silica sphere arrays become transparent. The reflected light of the bottom layer caused by the increasing refractive index contrast between the inside and outside of the hollow silica spheres could rapidly reveal the hidden information. The bilayer photonic crystal exhibits robust structural stability, and the hiding/revealing process is completely reversible, which shows great potential applications in steganography and information encryption.

MicroRNA-212 suppresses nonsmall lung cancer invasion and migration by regulating ubiquitin-specific protease-9.

MicroRNAs (miRNAs) play crucial roles in various biological processes, including migration, proliferation, differentiation, cell cycling, and apoptosis. Epithelial-mesenchymal transition (EMT) has been shown to be related to the capability of migration and invasion in many tumor cells. In this study, we used wound-healing assay and transwell invasion to analysis the capability of migration and invasion in non-small-cell lung carcinoma (NSCLC), respectively. The expression of ubiquitin-specific protease-9-X-linked (USP9X) and miR-212 messenger RNA (mRNA) was determined by quantitative real-time polymerase chain reaction and Western blot analysis was used to determine the E-cadherin and vimentin expression. Our results showed that miR-212 mimic inhibited cell migration and invasion, while miR-212 inhibitor increased cell migration and invasion. There was no significant difference between WP1130 and miR-212 mimic combined with WP1130 groups. Moreover, WP1130 inhibited the capability of the migration and invasion of NSCLC cells. Western blot analysis displayed that miR-212 mimic upregulated E-cadherin expression and downregulated vimentin expression, while miR-212 inhibitor downregulated E-cadherin and upregulated vimentin expression. These data showed that miR-212 regulated NSCLC cell invasion and migration by regulating USP9X expression. Taken together, these findings indicated that miR-212 regulated NSCLC cells migration and invasion through targeting USP9X involved in EMT.