Error Analysis and Optimization of Structural Parameters of Spatial Coordinate Testing System Based on Position-Sensitive Detector.

For the research on real-time accurate testing technology for the explosion point spatial coordinate of munitions, its currently commonly used methods such as acoustic-electric detection or high-speed imaging are limited by the field conditions, response rate, cost, and other factors. In this paper, a method of spatial coordinate testing for the explosion point based on a 2D PSD (position-sensitive detector) intersection is proposed, which has the advantages of a faster response, better real-time performance, and a lower cost. Firstly, a mathematical model of the spatial coordinate testing system was constructed, and an error propagation model for structural parameters was developed. The influence of the position of the optical axes' intersection as well as the azimuth angle and pitch angle on the test accuracy of the system was simulated and analyzed, thus obtaining the distribution and variation trend of the overall error propagation coefficient of the system. Finally, experiments were designed to obtain the test error of the system for validation. The results show that the system test accuracy is high when the azimuth angle is 20°-50°, the overall error propagation coefficient does not exceed 48.80, and the average test error is 56.17 mm. When the pitch angle is -2.5°-2.5°, the system has a higher test accuracy, with the overall error propagation coefficient not exceeding 44.82, and the average test error is 41.87 mm. The test accuracy of the system is higher when the position of the optical axes' intersection is chosen to make sure that explosion points fall in the region of the negative half-axis of the Zw-axis of the world coordinate system, with an overall error propagation coefficient of less than 44.78 and an average test error of 73.38 mm. It is shown that a reasonable selection of system structure parameters can significantly improve the system test accuracy and optimize the system deployment mode under the long-distance field conditions so as to improve the deployment efficiency.

Research on Target Deviation Measurement of Projectile Based on Shadow Imaging Method in Laser Screen Velocity Measuring System.

In the laser screen velocity measuring (LSVM) system, there is a deviation in the consistency of the optoelectronic response between the start light screen and the stop light screen. When the projectile passes through the light screen, the projectile's over-target position, at which the timing pulse of the LSVM system is triggered, deviates from the actual position of the light screen (i.e., the target deviation). Therefore, it brings errors to the measurement of the projectile's velocity, which has become a bottleneck, affecting the construction of a higher precision optoelectronic velocity measuring system. To solve this problem, this paper proposes a method based on high-speed shadow imaging to measure the projectile's target deviation, ΔS, when the LSVM system triggers the timing pulse. The infrared pulse laser is collimated by the combination of the aspherical lens to form a parallel laser source that is used as the light source of the system. When the projectile passes through the light screen, the projectile's over-target signal is processed by the specially designed trigger circuit. It uses the rising and falling edges of this signal to trigger the camera and pulsed laser source, respectively, to ensure that the projectile's over-target image is adequately exposed. By capturing the images of the light screen of the LSVM system and the over-target projectile separately, this method of image edge detection was used to calculate the target deviation, and this value was used to correct the target distance of the LSVM to improve the accuracy of the measurement of the projectile's velocity.

[Construction and evaluation of the property of decellular porcine aortic valve].

OBJECTIVE: To construct decellular porcine aortic valve (PAV) and to observe the existence of porcine endogenous retrovirus (PERV) and valve scaffold structure before and after implantation. METHODS: (1) Porcine aortic valve was obtained. The cellular components of PAV were completely removed by using detergent and nucleotidase solution combined with alteration of osmosis. (2) The decellular underwent HE staining and light microscopy and detection of its physical and chemical properties. (3) 20 pieces of decellular PAV were implanted into dogs. On e month later blood samples of the dogs were collected. PCR and RT-PCR were used to detect the PERV expression in 20 samples of pig's peripheral blood, 20 fresh PAVs, cultured pig kidney cells of the PK15 line (as positive control), decellular PAVs implanted into the dogs, and 10 samples of dogs' peripheral blood. (4) Small pieces of decellular PAVs were implanted into the subcutaneous tissues of 6 rabbits at the back, 6 pieces for one rabbit, and then extracted by the ends of the 4th, 6th, 8th and 10th week respectively after implantation to undergo HE staining and light microscopy. RESULTS: (1) Almost all cellular components in the PAVs had been removed after decellularization; the soluble protein contents lost markedly [(0.238 +/- 0.038)% vs. (0.484 +/- 0.116)%]; the water content of the decellular tissues increased significantly [(92.16 +/- 1.48)% vs. (89.2 +/- 1.55)%]; however, the decellular PAVs still maintained their excellent fibrous scaffold structure, and their shrinkage temperature and tension at fracture were not significantly changed [(72.0 +/- 0.7) degrees C vs. (71.2 +/- 0.8) degrees C, and (448.7 +/- 18.65)g/mm2 vs. (540.7 +/- 19.46)g/mm2 respectively]. (2) Agarose gel electrophoresis of all fresh PAVs and porcine peripheral blood samples showed a 219 bp band, which was 90% to 97% homologous with PERV-C gene, and the sequence of which is published in Medline. No 219 bp amplified band was found in all decellular PAVs and the peripheral blood samples of the dogs implanted with decellular PAV one month after the implantation. (3) The PAVs implanted in rabbit body showed very slight tissue reaction. Neutrophil, lymphocyte and plasmacyte infiltration were seen 4 weeks after; such inflammatory cell infiltration decreased markedly and the peripheral portions of the decellular PAVs began to be absorbed by the end of the 6th week after implantation. The decellular PAVs were completely absorbed without fibrosis or scar formation in the implantation area by the end of the 10th week. CONCLUSION: (1) The cellular components of PAV can be completely removed, the excellent fibrous scaffold structure and mechanical strength of aorta valve can be maintained, and the antigenicity is very weak. Subcutaneous implantation investigation shows that decellular PAV is an absorbable and degradable biological material. (2) There is PERV-C in PAV that can be removed after decellularization. PERV-C reaction is negative in the peripheral blood samples of the recipients implanted with decellular PAV.

[Construction of a tissue-engineered valve with decellular porcine aortic valve scaffold in the abdominal aorta of canine].

OBJECTIVE: To explore an experimental method for construction of tissue-engineered heart valve (TEHV) in canine abdominal aorta. METHODS: The decellular porcine aortic valve (PAV) leaflets seeded with canine vessel interstitial cells and endothelial cells (ECs) were implanted into 6 canine abdominal aortas. Valve specimens were obtained respectively at the end of 4, 6, 8 and 10 weeks after implantation were studied for morphology, histology and immunohistochemistry. RESULTS: (1) After 4 weeks implantation, multiple layers of cells grew into peripheral portion of valve scaffold, while new extracellular matrix appeared, and original scaffold tissue was partially absorbed. (2) At the end of 10th week after implantation, the decellular PAV scaffold disappeared completely and was substituted by recipient cells and new extracellular matrix. The interstitial cells in matrix was mainly consisted of fibroblasts and myofibroblast. The matrix was mainly composed by type I, III collagen, some elastic fibers with neutral and acid mucopolysaccharide. (3) Surface of valve leaflets were covered with endothelial cells. CONCLUSIONS: (1) TEHV is primarily constructed with recellularized PAV after implantation into canine abdominal aorta for 10 weeks. (2) Heterotopic implantation into the abdominal aorta is an alternative experimental procedure to study the TEHV.

Tissue-engineered heart valve on acellular aortic valve scaffold: in-vivo study.

The feasibility of constructing a tissue-engineered heart valve on an acellular porcine aortic valve leaflet was evaluated. A detergent and enzymatic extraction process was developed to remove the cellular components from porcine aortic valves. The acellular valve leaflets were seeded for 7 days in vitro with cells from canine arterial wall and endothelial cells. The constructs were implanted into the lumens of 6 canine abdominal aortas to assess the reconstruction of the valve leaflets. It was found that all cellular components had been removed from the porcine aortic valves. The valve leaflets were completely reconstructed at the end of the 10th week in vivo. Scanning electron microscopy showed that the valve leaflets were partially covered with endothelial cells. It was concluded that porcine aortic valves can be decellularized by the detergent and enzymatic extraction process and it is feasible to construct a tissue-engineered heart valve in vivo on an acellular valve scaffold.