Short-term anaerobic treatment maintained the quality of Actinidia arguta by activating the antioxidant defense system.

BACKGROUND: This study aimed to investigate the effect of 6, 12, and 24 h short-term anaerobic treatment on kiwiberry quality and antioxidant properties at 5 °C. RESULTS: Short-term anaerobic treatment was found to delay ripening and softening in kiwiberries, evident from changes in ethylene release, total soluble solids, starch, protopectin, and fruit texture. The 24 h treatment group exhibited the lowest decay rate of 12% on day 49, a 38% reduction compared with the control group. Anaerobic treatment reduced flesh translucency and decay in the fruit. The 12 h and 24 h treatments enhanced the activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, and increased the level of total phenolics, flavonoids, anthocyanins, and ascorbic acid. Moreover, it lowered oxidative damage in cell membranes, evidenced by reduced malondialdehyde content and relative conductivity. CONCLUSION: These results indicate that anaerobic treatment maintains the fruit quality by stimulating its antioxidant defense system. Therefore, short-term anaerobic treatment emerges as a promising method for kiwiberry storage. © 2024 Society of Chemical Industry.

Sparse reconstruction based on dictionary learning and group structure strategy for cone-beam X-ray luminescence computed tomography.

As a dual-modal imaging technology that has emerged in recent years, cone-beam X-ray luminescence computed tomography (CB-XLCT) has exhibited promise as a tool for the early three-dimensional detection of tumors in small animals. However, due to the challenges imposed by the low absorption and high scattering of light in tissues, the CB-XLCT reconstruction problem is a severely ill-conditioned inverse problem, rendering it difficult to obtain satisfactory reconstruction results. In this study, a strategy that utilizes dictionary learning and group structure (DLGS) is proposed to achieve satisfactory CB-XLCT reconstruction performance. The group structure is employed to account for the clustering of nanophosphors in specific regions within the organism, which can enhance the interrelation of elements in the same group. Furthermore, the dictionary learning strategy is implemented to effectively capture sparse features. The performance of the proposed method was evaluated through numerical simulations and in vivo experiments. The experimental results demonstrate that the proposed method achieves superior reconstruction performance in terms of location accuracy, target shape, robustness, dual-source resolution, and in vivo practicability.

Generalized conditional gradient method with adaptive regularization parameters for fluorescence molecular tomography.

Fluorescence molecular tomography (FMT) is an optical imaging technology with the ability of visualizing the three-dimensional distribution of fluorescently labelled probes in vivo. However, due to the light scattering effect and ill-posed inverse problems, obtaining satisfactory FMT reconstruction is still a challenging problem. In this work, to improve the performance of FMT reconstruction, we proposed a generalized conditional gradient method with adaptive regularization parameters (GCGM-ARP). In order to make a tradeoff between the sparsity and shape preservation of the reconstruction source, and to maintain its robustness, elastic-net (EN) regularization is introduced. EN regularization combines the advantages of L1-norm and L2-norm, and overcomes the shortcomings of traditional Lp-norm regularization, such as over-sparsity, over-smoothness, and non-robustness. Thus, the equivalent optimization formulation of the original problem can be obtained. To further improve the performance of the reconstruction, the L-curve is adopted to adaptively adjust the regularization parameters. Then, the generalized conditional gradient method (GCGM) is used to split the minimization problem based on EN regularization into two simpler sub-problems, which are determining the direction of the gradient and the step size. These sub-problems are addressed efficiently to obtain more sparse solutions. To assess the performance of our proposed method, a series of numerical simulation experiments and in vivo experiments were implemented. The experimental results show that, compared with other mathematical reconstruction methods, GCGM-ARP method has the minimum location error (LE) and relative intensity error (RIE), and the maximum dice coefficient (Dice) in the case of different sources number or shape, or Gaussian noise of 5%-25%. This indicates that GCGM-ARP has superior reconstruction performance in source localization, dual-source resolution, morphology recovery, and robustness. In conclusion, the proposed GCGM-ARP is an effective and robust strategy for FMT reconstruction in biomedical application.

Frequency importance analysis for chemical exchange saturation transfer magnetic resonance imaging using permuted random forest.

Chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) is a promising molecular imaging tool that allows sensitive detection of endogenous metabolic changes. However, because the CEST spectrum does not display a clear peak like MR spectroscopy, its signal interpretation is challenging, especially under 3-T field strength or with a large saturation B1 . Herein, as an alternative to conventional Z-spectral fitting approaches, a permuted random forest (PRF) method is developed to determine featured saturation frequencies for lesion identification, so-called CEST frequency importance analysis. Briefly, voxels in the CEST dataset were labeled as lesion and control according to multicontrast MR images. Then, by considering each voxel's saturation signal series as a sample, a permutation importance algorithm was employed to rank the contribution of saturation frequency offsets in the differentiation of lesion and normal tissue. Simulations demonstrated that PRF could correctly determine the frequency offsets (3.5 or -3.5 ppm) for classifying two groups of Z-spectra, under a range of B0 , B1 conditions and sample sizes. For ischemic rat brains, PRF only displayed high feature importance around amide frequency at 2 h postischemia, reflecting that the pH changes occurred at an early stage. By contrast, the data acquired at 24 h postischemia exhibited high feature importance at multiple frequencies (amide, water, and lipids), which suggested the complex tissue changes that occur during the later stages. Finally, PRF was assessed using 3-T CEST data from four brain tumor patients. By defining the tumor region on amide proton transfer-weighted images, PRF analysis identified different CEST frequency importance for two types of tumors (glioblastoma and metastatic tumor) (p < 0.05, with each image slice as a subject). In conclusion, the PRF method was able to rank and interpret the contribution of all acquired saturation offsets to lesion identification; this may facilitate CEST analysis in clinical applications, and open up new doors for comprehensive CEST analysis tools other than model-based approaches.

MRI-Based Radiomics Nomogram for Preoperative Differentiation Between Ocular Adnexal Lymphoma and Idiopathic Orbital Inflammation.

BACKGROUND: Ocular adnexal lymphoma (OAL) and idiopathic orbital inflammation (IOI) are malignant and benign lesions for which radiotherapy and corticosteroids are indicated, but similar clinical manifestations make their differentiation difficult. PURPOSE: To develop and validate an MRI-based radiomics nomogram for individual diagnosis of OAL vs. IOI. STUDY TYPE: Retrospective. POPULATION: A total of 103 patients (46.6% female) with mean age of 56.4 ± 16.3 years having OAL (n = 58) or IOI (n = 45) were divided into an independent training (n = 82) and a testing dataset (n = 21). FIELD STRENGTH/SEQUENCE: A 3-T, precontrast T1-weighted imaging (T1WI), T2-weighted imaging (T2WI), and postcontrast T1WI (T1 + C). ASSESSMENT: Radiomics features were extracted and selected from segmented tumors and peritumoral regions in MRI before-and-after filtering. These features, alone or combined with clinical characteristics, were used to construct a radiomics or joint signature to differentiate OAL from IOI, respectively. A joint nomogram was built to show the impact of the radiomics signature and clinical characteristics on individual risk of developing OAL. STATISTICAL TESTS: Area under the curve (AUC) and accuracy (ACC) were used for performance evaluation. Mann-Whitney U and Chi-square tests were used to analyze continuous and categorical variables. Decision curve analysis, kappa statistics, DeLong and Hosmer-Lemeshow tests were also conducted. P < 0.05 was considered statistically significant. RESULTS: The joint signature achieved an AUC of 0.833 (95% confidence interval [CI]: 0.806-0.870), slightly better than the radiomics signature with an AUC of 0.806 (95% CI: 0.767-0.838) (P = 0.778). The joint and radiomics signatures were comparable to experienced radiologists referencing to clinical characteristics (ACC = 0.810 vs. 0.796-0.806, P > 0.05) or not (AUC = 0.806 vs. 0.753-0.791, P > 0.05), respectively. The joint nomogram gained more net benefits than the radiomics nomogram, despite both showing good calibration and discriminatory efficiency (P > 0.05). DATA CONCLUSION: The developed radiomics-based analysis might help to improve the diagnostic performance and reveal the association between radiomics features and individual risk of developing OAL. EVIDENCE LEVEL: 3 TECHNICAL EFFICACY: 3.

Elastic net-based non-negative iterative three-operator splitting strategy for Cerenkov luminescence tomography.

Cerenkov luminescence tomography (CLT) provides a powerful optical molecular imaging technique for non-invasive detection and visualization of radiopharmaceuticals in living objects. However, the severe photon scattering effect causes ill-posedness of the inverse problem, and the location accuracy and shape recovery of CLT reconstruction results are unsatisfactory for clinical application. Here, to improve the reconstruction spatial location accuracy and shape recovery ability, a non-negative iterative three operator splitting (NNITOS) strategy based on elastic net (EN) regularization was proposed. NNITOS formalizes the CLT reconstruction as a non-convex optimization problem and splits it into three operators, the least square, L1/2-norm regularization, and adaptive grouping manifold learning, then iteratively solved them. After stepwise iterations, the result of NNITOS converged progressively. Meanwhile, to speed up the convergence and ensure the sparsity of the solution, shrinking the region of interest was utilized in this strategy. To verify the effectiveness of the method, numerical simulations and in vivo experiments were performed. The result of these experiments demonstrated that, compared to several methods, NNITOS can achieve superior performance in terms of location accuracy, shape recovery capability, and robustness. We hope this work can accelerate the clinical application of CLT in the future.

A deep learning model combining multimodal radiomics, clinical and imaging features for differentiating ocular adnexal lymphoma from idiopathic orbital inflammation.

OBJECTIVES: To evaluate the value of deep learning (DL) combining multimodal radiomics and clinical and imaging features for differentiating ocular adnexal lymphoma (OAL) from idiopathic orbital inflammation (IOI). METHODS: Eighty-nine patients with histopathologically confirmed OAL (n = 39) and IOI (n = 50) were divided into training and validation groups. Convolutional neural networks and multimodal fusion layers were used to extract multimodal radiomics features from the T1-weighted image (T1WI), T2-weighted image, and contrast-enhanced T1WI. These multimodal radiomics features were then combined with clinical and imaging features and used together to differentiate between OAL and IOI. The area under the curve (AUC) was used to evaluate DL models with different features under five-fold cross-validation. The Student t-test, chi-squared, or Fisher exact test was used for comparison of different groups. RESULTS: In the validation group, the diagnostic AUC of the DL model using combined features was 0.953 (95% CI, 0.895-1.000), higher than that of the DL model using multimodal radiomics features (0.843, 95% CI, 0.786-0.898, p < 0.01) or clinical and imaging features only (0.882, 95% CI, 0.782-0.982, p = 0.13). The DL model built on multimodal radiomics features outperformed those built on most bimodalities and unimodalities (p < 0.05). In addition, the DL-based analysis with the orbital cone area (covering both the orbital mass and surrounding tissues) was superior to that with the region of interest (ROI) covering only the mass area, although the difference was not significant (p = 0.33). CONCLUSIONS: DL-based analysis that combines multimodal radiomics features with clinical and imaging features may help to differentiate between OAL and IOI. KEY POINTS: • It is difficult to differentiate OAL from IOI due to the overlap in clinical and imaging manifestations. • Radiomics has shown potential for noninvasive diagnosis of different orbital lymphoproliferative disorders. • DL-based analysis combining radiomics and imaging and clinical features may help the differentiation between OAL and IOI.

Morita therapy for obsessive-compulsive disorder: A systematic review and meta-analysis.

OBJECTIVE: To assess the clinical efficacy of Morita therapy in combination with pharmacotherapy in adults who were diagnosed with current OCDs. METHODS: We searched 10 databases to identify articles written in English or Chinese that were published until 15 April 2021. Randomized controlled trials were included. Two authors of this review independently selected the studies, assessed the risk of bias, and extracted the data. RESULTS: Twenty-one studies with a total of 1604 participants met the inclusion criteria. Morita therapy plus pharmacotherapy was significantly superior to pharmacotherapy alone in the efficiency of OCD (RR = 1.34, 95% CI: 1.26 to 1.44, I2 = 0%), and better in reducing OCD severity symptoms (MD = -3.55, 95% CI: -4.34 to -2.75, I2 = 80%). CONCLUSION: Our meta-analysis and systematic review suggest that Morita therapy may be an effective approach to improve OCDs.

Bag-of-features-based radiomics for differentiation of ocular adnexal lymphoma and idiopathic orbital inflammation from contrast-enhanced MRI.

OBJECTIVES: To evaluate the effectiveness of bag-of-features (BOF)-based radiomics for differentiating ocular adnexal lymphoma (OAL) and idiopathic orbital inflammation (IOI) from contrast-enhanced MRI (CE-MRI). METHODS: Fifty-six patients with pathologically confirmed IOI (28 patients) and OAL (28 patients) were randomly divided into training (n = 42) and testing (n = 14) groups. One hundred sixty texture features extracted from the CE-MR image were encoded into the BOF representation with fewer features. The support vector machine (SVM) with a linear kernel was used as the classifier. Data augmented was performed by cropping orbital lesions in different directions to alleviate the over-fitting problem. Student's t test and the Holm-Bonferroni method were employed to compare the performance of different analysis methods. The chi-square test was used to compare the analysis with MRI and human radiological diagnosis. RESULTS: In the independent testing group, the differentiation by the BOF features with augmentation achieved an area under the curve (AUC) of 0.803 (95% CI: 0.725-0.880), which was significantly higher than that of the BOF features without augmentation and that of the texture features (p < 0.05). In addition, the same radiomic analysis with pre-contrast MRI obtained an AUC of 0.618 (95% CI: 0.560-0.677), which was significantly lower than that with CE-MRI. The diagnostic performance of the analysis with CE-MRI was significantly better than the radiology resident (p < 0.05) but had no significant difference with the experienced radiologist, even though there was less consistency between the radiomic analysis and the human visual diagnosis. CONCLUSIONS: The BOF-based radiomics may be helpful for the differentiation between OAL and IOI. KEY POINTS: • It is challenging to differentiate OAL from IOI due to the similar clinical and image features. • Radiomics has great potential for the noninvasive diagnosis of orbital diseases. • The BOF representation from patch to image may help the differentiation of OAL and IOI.

Simplification method for 3D Terracotta Warrior fragments based on local structure and deep neural networks.

The emergence of the three-dimensional (3D) scanner has greatly benefited archeology, which can now store cultural heritage artifacts in computers and present them on the Internet. As many Terracotta Warriors have been predominantly found in fragments, the pre-processing of these fragments is very important. The raw point cloud of the fragments has lots of redundant points; it requires an excessively large storage space and much time for post-processing. Thus, an effective method for point cloud simplification is proposed for 3D Terracotta Warrior fragments. First, an algorithm for extracting feature points is proposed that is based on local structure. By constructing a k-dimension tree to establish the k-nearest neighborhood of the point cloud, and comparing the feature discriminant parameter and characteristic threshold, the feature points, as well as the non-feature points, are separated. Second, a deep neural network is constructed to simplify the non-feature points. Finally, the feature points and the simplified non-feature points are merged to form the complete simplified point cloud. Experiments with the public point cloud data and the real-world Terracotta Warrior fragments data are designed and conducted. Excellent simplification results were obtained, indicating that the geometric feature can be preserved very well.

Feasibility study of three-dimensional multiple-beam x-ray luminescence tomography.

X-ray luminescence tomography (XLT) is a promising imaging technology based on x-ray beams, with high-resolution capability. We developed a fan-beam XLT system, where the x-ray beam scans the object at predefined directions and positions. As the scanning at one position needs to cover the object, the data acquisition time is usually long. To improve spatial resolution, we propose a three-dimensional multiple-beam x-ray luminescence imaging method, in which the x rays are modulated by an x-ray fence-modulation component. The proposed method can produce multiple x-ray beams and ensure spatial resolution along the longitudinal direction as well as the transverse plane. The proposed methods of single-source experiments can achieve 0.62 mm in location error and 0.87 in the dice coefficient while 1.32 mm in location error and 0.63 in the dice coefficient in the double-source experiment. The simulation experiments show that our proposed method can achieve better results at different depths than the traditional scanning method. It is also demonstrated that the best simulation results can be achieved with the smallest x-ray width.

Fire behavior and smoke modeling: Model improvement and measurement needs for next-generation smoke research and forecasting systems.

There is an urgent need for next-generation smoke research and forecasting (SRF) systems to meet the challenges of the growing air quality, health, and safety concerns associated with wildland fire emissions. This review paper presents simulations and experiments of hypothetical prescribed burns with a suite of selected fire behavior and smoke models and identifies major issues for model improvement and the most critical observational needs. The results are used to understand the new and improved capability required for the next-generation SRF systems and to support the design of the Fire and Smoke Model Evaluation Experiment (FASMEE) and other field campaigns. The next-generation SRF systems should have more coupling of fire, smoke, and atmospheric processes to better simulate and forecast vertical smoke distributions and multiple sub-plumes, dynamical and high-resolution fire processes, and local and regional smoke chemistry during day and night. The development of the coupling capability requires comprehensive and spatially and temporally integrated measurements across the various disciplines to characterize flame and energy structure (e.g., individual cells, vertical heat profile and the height of well mixing flaming gases), smoke structure (vertical distributions and multiple sub-plumes), ambient air processes (smoke eddy, entrainment and radiative effects of smoke aerosols), fire emissions (for different fuel types and combustion conditions from flaming to residual smoldering), as well as night-time processes (smoke drainage and super-fog formation).

Molecular characterization and expression analyses of ST8Sia II and IV in piglets during postnatal development: lack of correlation between transcription and posttranslational levels.

The two mammalian α2,8-polysialyltransferases (polyST's), ST8Sia II (STX) and ST8Sia IV (PST), catalyze synthesis of the α2-8-linked polysialic acid (polySia) glycans on neural cell adhesion molecules (NCAMs). The objective of this study was to clone the coding sequence of the piglet ST8Sia II and determine the mRNA expression levels of ST8Sia II, ST8Sia IV, NCAM and neuropilin-2 (NRP-2), also a carrier protein of polySia, during postnatal development. The amino acid sequence deduced from the coding sequence of ST8Sia II was compared with seven other mammalian species. Piglet ST8Sia II was highly conserved and shared 67.8% sequence identity with ST8Sia IV. Genes coding for ST8Sia II and IV were differentially expressed and distinctly different in neural and non-neural tissues at postnatal days 3 and 38. Unexpectedly, the cellular levels of mRNA coding for ST8Sia II and IV showed no correlation with the posttranslational level of polySia glycans in different tissues. In contrast, mRNA abundance coding for NCAM and neuropilin-2 correlated with expression of ST8Sia II and IV. These findings show that the cellular abundance of ST8Sia II and IV in postnatal piglets is regulated at the level of translation/posttranslation, and not at the level of transcription, a finding that has not been previously reported. These studies further highlight differences in the molecular mechanisms controlling polysialylation in adult rodents and neonatal piglets.

SODL-IR-FISTA: sparse online dictionary learning with iterative reduction FISTA for cone-beam X-ray luminescence computed tomography.

Cone beam X-ray luminescence computed tomography (CB-XLCT) is an emerging imaging technique with potential for early 3D tumor detection. However, the reconstruction challenge due to low light absorption and high scattering in tissues makes it a difficult inverse problem. In this study, the online dictionary learning (ODL) method, combined with iterative reduction FISTA (IR-FISTA), has been utilized to achieve high-quality reconstruction. Our method integrates IR-FISTA for efficient and accurate sparse coding, followed by an online stochastic approximation for dictionary updates, effectively capturing the sparse features inherent to the problem. Additionally, a re-sparse step is introduced to enhance the sparsity of the solution, making it better suited for CB-XLCT reconstruction. Numerical simulations and in vivo experiments were conducted to assess the performance of the method. The SODL-IR-FISTA achieved the smallest location error of 0.325 mm in in vivo experiments, which is 58% and 45% of the IVTCG-L 1 (0.562 mm) and OMP-L 0 (0.721 mm), respectively. Additionally, it has the highest DICE similarity coefficient, which is 0.748. The results demonstrate that our approach outperforms traditional methods in terms of localization precision, shape restoration, robustness, and practicality in live subjects.

CORONet: A Cross-Sequence Joint Representation and Hypergraph Convolutional Network for Classifying Molecular Subtypes of Breast Cancer Using Incomplete DCE-MRI.

Breast cancer, the predominant malignancy among women, is characterized by significant heterogeneity, leading to the emergence of distinct molecular subtypes. Accurate differentiation of these molecular subtypes holds paramount clinical significance, owing to substantial variations in prognosis, therapeutic strategies, and survival outcomes. In this study, we propose a cross-sequence joint representation and hypergraph convolution network (CORONet) for classifying molecular subtypes of breast cancer using incomplete DCE-MRI. Specifically, we first build a cross-sequence joint representation (COR) module to integrate image imputation and feature representation into a unified framework, encouraging effective feature extraction for subsequent classification. Then, we fuse multiple COR features and applied feature selection to reduce the redundant information between sequences. Finally, we deploy hypergraph structures to model high-order correlation among different subjects and extracted high-level semantic features by hypergraph convolutions for molecular subtyping. Extensive experiments on incomplete DCE-MRIs of 395 patients from the TCIA repository showed a significant improvement of our CORONet over state of the arts, with the area under the curve (AUC) of 0.891 and 0.903 for luminal and triple-negative (TN) subtype prediction, respectively. Similar advantages of CORONet were also confirmed in partial complete DCE-MRIs of 144 patients, achieving an AUC of 0.858 and 0.832 for predicting luminal and TN subtypes of breast cancer, respectively. Nevertheless, both of these values were lower compared to the scenario where DCE-MRIs from all 395 patients were utilized. Our study contributes to the precise molecular subtyping using incomplete multi-sequence DCE-MRI, thereby offering promising prospects for future risk stratification of breast cancer patients.

A Deep Learning Model for Predicting Molecular Subtype of Breast Cancer by Fusing Multiple Sequences of DCE-MRI From Two Institutes.

RATIONALE AND OBJECTIVES: To evaluate the performance of deep learning (DL) in predicting different breast cancer molecular subtypes using DCE-MRI from two institutes. MATERIALS AND METHODS: This retrospective study included 366 breast cancer patients from two institutes, divided into training (n = 292), validation (n = 49) and testing (n = 25) sets. We first transformed the public DCE-MRI appearance to ours to alleviate small-data-size and class-imbalance issues. Second, we developed a multi-branch convolutional-neural-network (MBCNN) to perform molecular subtype prediction. Third, we assessed the MBCNN with different regions of interest (ROIs) and fusion strategies, and compared it to previous DL models. Area under the curve (AUC) and accuracy (ACC) were used to assess different models. Delong-test was used for the comparison of different groups. RESULTS: MBCNN achieved the optimal performance under intermediate fusion and ROI size of 80 pixels with appearance transformation. It outperformed CNN and convolutional long-short-term-memory (CLSTM) in predicting luminal B, HER2-enriched and TN subtypes, but without demonstrating statistical significance except against CNN in TN subtypes, with testing AUCs of 0.8182 vs. [0.7208, 0.7922] (p=0.44, 0.80), 0.8500 vs. [0.7300, 0.8200] (p=0.36, 0.70) and 0.8900 vs. [0.7600, 0.8300] (p=0.03, 0.63), respectively. When predicting luminal A, MBCNN outperformed CNN with AUCs of 0.8571 vs. 0.7619 (p=0.08) without achieving statistical significance, and is comparable to CLSTM. For four-subtype prediction, MBCNN achieved an ACC of 0.64, better than CNN and CLSTM models with ACCs of 0.48 and 0.52, respectively. CONCLUSION: Developed DL model with the feature extraction and fusion of DCE-MRI from two institutes enabled preoperative prediction of breast cancer molecular subtypes with high diagnostic performance.

Disappearing lakes in semiarid Northern China: drivers and environmental impact.

The widely distributed 241 lakes in the semiarid region of China bordering the Asian Gobi desert provide an irreplaceable environment for the region's human inhabitants, livestock, and wildlife. Using satellite imagery, we tracked the changing areas of lake water and freshwater/salty marshes during the last four decades and correlated observed changes with concurrent temperature and precipitation. On average, most of the lake size groups across different subregions showed a reduction in area from the 1970s to 2000s, particularly from the 1990s to 2000s (P < 0.05); 121 of the 241 lakes became fully desiccated at the end of the 2000s. Our results confirmed the prevalence of drought-induced lake shrinkage and desiccation at a regional scale, which has been sustained since the year 2000, and highlighted an accelerated shrinkage of individual lakes by human water use in the agriculture-dominated regions. Lake waters have become salinized, and freshwater marsh has been replaced by salty marsh, threatening the populations of endangered waterfowl species such as the red-crowned crane as well as the aquatic ecosystem. Although the dry lakebeds are a potential source of dust, the establishment of salty marsh on bare lake beds could have partially reduced dust release due to the increase in vegetation cover.

Seasonal differences in the spatial patterns of wildfire drivers and susceptibility in the southwest mountains of China.

Wildfires directly affect global ecosystem stability and severely threaten human life. The mountainous areas of Southwest China experience frequent wildfires. Mapping the susceptibility patterns and analyzing the drivers of wildfires are crucial for effective wildfire management, especially considering that the inclusion of seasonal dimensions will produce more dynamic results. Using Yunnan Province of China as a case study area, a method was attempted to distinguish dependable wildfires by season, while possible wildfire drivers were obtained and refined within seasons. The patterns of wildfire susceptibility in different seasons were modeled based on the Maxent and random forest models. Then, the spatial relationships between wildfire and potential drivers were analyzed integrating with GeoDetector to evaluate the variable importance of drivers and the marginal effect of drivers. The results showed that the two models effectively depicted each season's wildfire susceptibility. The susceptible wildfire areas in spring and winter are located throughout Yunnan Province, with anthropogenic factors being the most significant drivers. During the summer and autumn, wildfire risk areas are relatively concentrated, showing a trend of dominant drought-driven and humid conditions. The differences in wildfire drivers across seasons reflect the lagged effect of climate factors on wildfires, leading to significant discrepancies in the marginal effects of how seasonal drivers affect wildfires. The findings improve our understanding of the effects of the interseasonal variability of environmental variables on wildfires and promote the development of specific seasonal wildfire management strategies.

Self-Supervised Triplet Contrastive Learning for Classifying Endometrial Histopathological Images.

Early identification of endometrial cancer or precancerous lesions from histopathological images is crucial for precise endometrial medical care, which however is increasing hampered by the relative scarcity of pathologists. Computer-aided diagnosis (CAD) provides an automated alternative for confirming endometrial diseases with either feature-engineered machine learning or end-to-end deep learning (DL). In particular, advanced self-supervised learning alleviates the dependence of supervised learning on large-scale human-annotated data and can be used to pre-train DL models for specific classification tasks. Thereby, we develop a novel self-supervised triplet contrastive learning (SSTCL) model for classifying endometrial histopathological images. Specifically, this model consists of one online branch and two target branches. The second target branch includes a simple yet powerful augmentation module named random mosaic masking (RMM), which functions as an effective regularization by mapping the features of masked images close to those of intact ones. Moreover, we add a bottleneck Transformer (BoT) model into each branch as a self-attention module to learn the global information by considering both content information and relative distances between features at different locations. On public endometrial dataset, our model achieved four-class classification accuracies of 77.31 ± 0.84, 80.87 ± 0.48 and 83.22 ± 0.87% using 20, 50 and 100% labeled images, respectively. When transferred to the in-house dataset, our model obtained a three-class diagnostic accuracy of 96.81% with 95% confidence interval of 95.61-98.02%. On both datasets, our model outperformed state-of-the-art supervised and self-supervised methods. Our model may help pathologists to automatically diagnose endometrial diseases with high accuracy and efficiency using limited human-annotated histopathological images.

Regularized reconstruction based on joint smoothly clipped absolute deviation regularization and graph manifold learning for fluorescence molecular tomography.

Objective.Fluorescence molecular tomography (FMT) is an optical imaging modality that provides high sensitivity and low cost, which can offer the three-dimensional distribution of biomarkers by detecting the fluorescently labeled probe noninvasively. In the field of preclinical cancer diagnosis and treatment, FMT has gained significant traction. Nonetheless, the current FMT reconstruction results suffer from unsatisfactory morphology and location accuracy of the fluorescence distribution, primarily due to the light scattering effect and the ill-posed nature of the inverse problem.Approach.To address these challenges, a regularized reconstruction method based on joint smoothly clipped absolute deviation regularization and graph manifold learning (SCAD-GML) for FMT is presented in this paper. The SCAD-GML approach combines the sparsity of the fluorescent sources with the latent manifold structure of fluorescent source distribution to achieve more accurate and sparse reconstruction results. To obtain the reconstruction results efficiently, the non-convex gradient descent iterative method is employed to solve the established objective function. To assess the performance of the proposed SCAD-GML method, a comprehensive evaluation is conducted through numerical simulation experiments as well asin vivoexperiments.Main results.The results demonstrate that the SCAD-GML method outperforms other methods in terms of both location and shape recovery of fluorescence biomarkers distribution.Siginificance.These findings indicate that the SCAD-GML method has the potential to advance the application of FMT inin vivobiological research.