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1.
Appl Microbiol Biotechnol ; 107(13): 4187-4197, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37233756

RESUMO

Non-ribosomal peptide synthetases are mega-enzyme assembly lines that synthesize many clinically useful compounds. As a gatekeeper, they have an adenylation (A)-domain that controls substrate specificity and plays an important role in product structural diversity. This review summarizes the natural distribution, catalytic mechanism, substrate prediction methods, and in vitro biochemical analysis of the A-domain. Taking genome mining of polyamino acid synthetases as an example, we introduce research on mining non-ribosomal peptides based on A-domains. We discuss how non-ribosomal peptide synthetases can be engineered based on the A-domain to obtain novel non-ribosomal peptides. This work provides guidance for screening non-ribosomal peptide-producing strains, offers a method to discover and identify A-domain functions, and will accelerate the engineering and genome mining of non-ribosomal peptide synthetases. KEY POINTS: • Introducing adenylation domain structure, substrate prediction, and biochemical analysis methods • Advances in mining homo polyamino acids based on adenylation domain analysis • Creating new non-ribosomal peptides by engineering adenylation domains.


Assuntos
Peptídeo Sintases , Peptídeos , Peptídeos/química , Peptídeo Sintases/metabolismo , Especificidade por Substrato
2.
Appl Microbiol Biotechnol ; 104(8): 3627-3641, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32078018

RESUMO

Plants associate with numerous microbes, but little is known about how microbiome components, especially fungi, adapt to specific plant compartments. The adaptability of microbial function to the plant compartment is also not clear especially for woody species. Here, we characterized the bacterial and fungal communities in root endosphere, stems, and rhizospheres of 33 Broussonetia papyrifera seedlings, based on amplification of 16S and ITS rRNA. Results showed that the α-diversity indexes of the bacterial community were significantly different in different plant compartments and they significantly increased from stem to root endosphere to the rhizosphere, whereas those of the fungal community were similar (p > 0.05). However, the result of constrained PCoA (CPCoA) and analysis of similarity (ANOSIM) showed that both bacterial and fungal compositions were significantly affected by plant compartments (p < 0.01). In detail, the operational taxonomic units (OTUs) distribution of the bacterial community was significantly different, but 249 of 252 fungal OTUs were shared in different plant compartments. Both the bacterial and fungal compositions were significantly influenced by plant compartments, based on the result on phyla, core OTUs, and indicator OTUs level. Further, 40 of 42 enriched KEGG pathways involving the bacteria also differed significantly among plant compartments (p < 0.01). This study provides an understanding of the influence of plant compartments on the microbiome and confirms that the disperse limitation of fungal OTUs across different plant compartments is smaller. This study sheds light on how the microbial community adapts to and thrives in different plant compartments.


Assuntos
Broussonetia/anatomia & histologia , Broussonetia/microbiologia , Microbiota , Rizosfera , Bactérias/classificação , Bactérias/metabolismo , Fungos/classificação , Fungos/fisiologia , Raízes de Plantas/microbiologia , Caules de Planta/microbiologia
3.
Appl Microbiol Biotechnol ; 103(9): 3899-3913, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30903216

RESUMO

Few studies have investigated the effect of environment on the root-associated microbiome, especially for woody plants in their native environment. The roots and rhizosphere soils of a native woody species (Broussonetia papyrifera) sampled across four different climate types in China were used to elucidate the influence of environment on the root-associated microbiome. Our results showed that the B. papyrifera root-associated microbiome contained abundant Proteobacteria and Basidiomycota, especially Pseudomonas and Rhizobium. The root-associated microbiomes were found to be significantly different under different climate types except for the bacterial community in the rhizosphere, and the proportion of bacterial operational taxonomic units (OTUs) shared among different climate types was lower than that of fungi. More than 50% of the total variance between microbiomes could be explained by 15 environmental factors, six of which, especially soil concentration phosphate and nitrate, had a significant effect. This study provided a comprehensive understanding of the root-associated microbiome of B. papyrifera and further confirmed the effect of environment on the root-associated microbiome of B. papyrifera under different climate types, with some exceptions in the rhizobacterial community and fungal OTUs. Our findings advanced knowledge of the effect of environment through an exploration of environmental factors and found that the nitrogen and phosphorus content represented the key factors.


Assuntos
Bactérias/isolamento & purificação , Broussonetia/microbiologia , Fungos/isolamento & purificação , Microbiota , Raízes de Plantas/microbiologia , Microbiologia do Solo , Bactérias/classificação , Bactérias/genética , China , Clima , Ecossistema , Fungos/classificação , Fungos/genética , Filogenia , Rizosfera
4.
Biotechnol Lett ; 40(3): 455-464, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29299715

RESUMO

Itaconic acid, 2-methylidenebutanedioic acid, is a precursor of polymers, chemicals, and fuels. Many fungi can synthesize itaconic acid; Aspergillus terreus and Ustilago maydis produce up to 85 and 53 g l-1, respectively. Other organisms, including Aspergillus niger and yeasts, have been engineered to produce itaconic acid. However, the titer of itaconic acid is low compared with the analogous major fermentation product, citric acid, for which the yield is > 200 g l-1. Here, we review two types of pathway for itaconic acid biosynthesis as well as recent advances by metabolic engineering strategies and process optimization to enhance itaconic acid productivity in native producers and heterologous hosts. We also propose further improvements to overcome existing problems.


Assuntos
Reatores Biológicos/microbiologia , Engenharia Metabólica , Succinatos/metabolismo , Aspergillus/genética , Aspergillus/metabolismo , Redes e Vias Metabólicas , Ustilago/genética , Ustilago/metabolismo
5.
Fish Physiol Biochem ; 42(2): 407-21, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26494141

RESUMO

The pituitary adenylate cyclase activating polypeptide (PACAP) is a new type of hypophysiotropic hormone and plays an important role in regulating the synthesis and secretion of growth hormone and gonadotropin. The research on the relationship between PACAP and different growth traits would contribute to explain its function during the process of growth. Moreover, epigenetic modifications, especially DNA methylation at the CpG sites of the SNPs, play important roles in regulating gene expression. The results suggest that a SNP mutation (c.C151G) in the PACAP gene of male half smooth tongue sole (Cynoglossus semilaevis) is significantly associated with growth traits and serum physiological and biochemical parameters such as inorganic phosphorus (P < 0.05). The SNP is located in a CpG-rich region of exon 1. Intriguingly, the transition (C→G) added a new methylation site of PACAP gene. This SNP was also significantly related to the expression and methylation level of PACAP (P < 0.05). Individuals with GG genotype had faster growth rates than those of CG and CC genotypes. Moreover, GG genotype had significantly higher PACAP expression level and lower methylation level than CG and CC genotypes. In the serum indexes, only inorganic phosphorus content within GG genotypes was significantly higher than CC genotypes. This implied that the mutation and methylation status of PACAP gene could influence growth traits and this locus could be considered as a candidate genetic or epigenetic marker for Cynoglossus semilaevis molecular breeding.


Assuntos
Linguados/fisiologia , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/genética , RNA Mensageiro/metabolismo , Animais , Metilação de DNA , Éxons , Linguados/metabolismo , Expressão Gênica , Gonadotropinas/metabolismo , Hormônio do Crescimento , Masculino , Fenótipo , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/metabolismo , Polimorfismo Genético
6.
Environ Microbiol ; 17(5): 1560-73, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25059531

RESUMO

The Bacillus cereus group is composed of Gram-positive spore-forming bacteria of clinical and ecological importance. More than 200 B. cereus group isolates have been sequenced. However, there are few reports of B. cereus group antibiotic resistance genes. This study identified two functional classes of macrolide phosphotransferases (Mphs) in the B. cereus group. Cluster A Mphs inactivate 14- and 15-membered macrolides while Cluster B Mphs inactivate 14-, 15- and 16-membered compounds. The genomic region surrounding the Cluster B Mph gene is related to various plasmid sequences, suggesting that this gene is an acquired resistance gene. In contrast, the Cluster A Mph gene is located in a chromosomal region conserved among all B. cereus group isolates, and data indicated that it was acquired early in the evolution of the group. Therefore, the Cluster A gene can be considered an intrinsic resistance gene. However, the gene itself is not present in all strains and our comparative genomics analyses showed that it is exchanged among strains of the B. cereus group by the mean of homologous recombination. These results provide an alternative mechanism to intrinsic resistance.


Assuntos
Bacillus cereus/enzimologia , Bacillus cereus/metabolismo , Farmacorresistência Bacteriana/genética , Macrolídeos/farmacologia , Fosfotransferases/genética , Antibacterianos/farmacologia , Bacillus cereus/classificação , Bacillus cereus/genética , Sequência de Bases , Genômica , Família Multigênica , Filogenia , Plasmídeos/genética , Esporos Bacterianos/genética
7.
Int J Syst Evol Microbiol ; 64(Pt 3): 952-956, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24623820

RESUMO

A halophilic archaeal strain, GX48(T), was isolated from the Gangxi marine solar saltern near Weihai city in Shandong Province, China. Cells of the strain were rod-shaped, stained Gram-negative and formed red-pigmented colonies. Strain GX48(T) was able to grow at 25-50 °C (optimum 37 °C), in the presence of 1.4-4.8 M NaCl (optimum 2.6 M NaCl), with 0-1.0 M MgCl2 (optimum 0.05 M MgCl2) and at pH 5.5-9.5 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to those of Natronoarchaeum mannanilyticum YSM-123(T) and Natronoarchaeum philippinense 294-194-5(T). 16S rRNA gene analysis revealed that strain GX48(T) had two dissimilar 16S rRNA genes and both of them were phylogenetically related to those of the two current members of the genus Natronoarchaeum (96.2-98.3 % similarities). The rpoB' gene sequence similarities between strain GX48(T) and Natronoarchaeum mannanilyticum YSM-123(T) and Natronoarchaeum philippinense 294-194-5(T) were 96.0 % and 94.7 %, respectively. The DNA G+C content of strain GX48(T) was 66.2 mol%. Strain GX48(T) showed low DNA-DNA relatedness with the two members of the genus Natronoarchaeum. It was concluded that strain GX48(T) ( = CGMCC 1.10388(T) = JCM 17119(T)) represents a novel species of the genus Natronoarchaeum, for which the name Natronoarchaeum rubrum sp. nov. is proposed. An emended description of the genus Natronoarchaeum is also presented.


Assuntos
Halobacteriaceae/classificação , Filogenia , Microbiologia da Água , Composição de Bases , China , DNA Arqueal/genética , Genes Bacterianos , Glicolipídeos/química , Halobacteriaceae/genética , Halobacteriaceae/isolamento & purificação , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Fosfatidilgliceróis/química , Pigmentação , RNA Ribossômico 16S/genética , Sais
8.
Curr Microbiol ; 68(2): 156-60, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24048696

RESUMO

Halophilic archaeal strain GX31(T) was isolated from a marine solar saltern of China. The cells of the strain were rod-shaped and lysed in distilled water, stain Gram-negative and formed red-pigmented colonies. It was neutrophilic, and required at least 0.9 M NaCl and 0-1.0 M MgCl2 for growth under the optimum growth temperature of 37 °C. The major polar lipids of the strain were phosphatidylglycerol (PG), PG phosphate methyl ester, PG sulphate, and two major glycolipids chromatographically identical to sulphated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified lipids were also detected. On the basis of 16S rRNA gene sequence analysis, strain GX31(T) was closely related to the members of Halobellus of the family Halobacteriaceae with similarities of 94.1-98.7 %. Strain GX31(T) showed 89.8-95.4 % of the rpoB' gene similarity to the members of Halobellus. The DNA G+C content of strain GX31(T) was 66.8 mol%. Strain GX31(T) showed low DNA-DNA relatedness with two most related members of the genus Halobellus. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain GX31(T) represent a novel species of the genus Halobellus, for which the name Halobellus litoreus sp. nov. is proposed. The type strain is GX31(T) (=CGMCC 1.10387(T) = JCM 17118(T)).


Assuntos
Halobacteriaceae/classificação , Halobacteriaceae/isolamento & purificação , RNA Bacteriano , Halobacteriaceae/metabolismo , Lipídeos , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S
9.
Arch Microbiol ; 195(12): 799-803, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24129619

RESUMO

Halophilic archaeal strain YGH66(T) was isolated from the Yinggehai marine solar saltern near the Sanya city of Hainan Province, China. Cells were pleomorphic, flat, stained Gram-negative, and produced pink-pigmented colonies. Strain YGH66(T) was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.1 M NaCl), at 0.005-1.0 M MgCl2 (optimum 0.05 M MgCl2), and at pH 6.0-8.0 (optimum pH 7.0). The cells of strain YGH66(T) were lysed in distilled water, and the minimum NaCl concentration that prevented cell lysis was 5 % (w/v). The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, one major glycolipid (GL1) chromatographically identical to sulfated mannosyl glucosyl diether and a minor unidentified lipid (GL2), respectively. On the basis of 16S rRNA gene sequence analysis, strain YGH66(T) was closely related to Haloplanus natans JCM 14081(T), Haloplanus aerogenes TBN37(T), and Haloplanus vescus RO5-8(T) with the similarities of 98.0, 97.6, and 96.9 %, respectively. The rpoB' gene similarity between strain YGH66(T) and the current three members of Haloplanus were 90.4-92.8 %. The DNA G+C content of strain YGH66(T) was 67.2 mol %. The DNA-DNA hybridization values between strain YGH66(T) and three members of Haloplanus, H. natans JCM 14081(T), H. aerogenes TBN37(T), H. vescus RO5-8(T) were 50, 46 and 39 %, respectively. It was concluded that strain YGH66(T) represents a novel species of the genus Haloplanus, for which the name Haloplanus salinus sp. nov. is proposed. The type strain is YGH66(T) (=CGMCC 1.12127(T) = JCM 18368(T)).


Assuntos
Halobacteriaceae/classificação , Halobacteriaceae/fisiologia , Proteínas de Bactérias/genética , Composição de Bases , China , DNA Arqueal/genética , Glicolipídeos/metabolismo , Halobacteriaceae/genética , Halobacteriaceae/isolamento & purificação , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Cloreto de Sódio/metabolismo , Especificidade da Espécie
10.
Int J Syst Evol Microbiol ; 63(Pt 11): 3975-3980, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23728369

RESUMO

Two halophilic archaeal strains, YC20(T) and XD15, were isolated from a marine solar saltern and an inland salt lake in China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They were neutrophilic, requiring at least 100 g NaCl l(-1) and 0.5-95 g MgCl2 l(-1) for growth at the optimum growth temperature of 37 °C. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified glycolipids were also detected. The 16S rRNA gene sequences of the two strains were 99.5 % identical and showed 94.0-95.9 % similarity to the most closely related members of the genus Halobellus of the family Halobacteriaceae. The rpoB' gene sequence similarity between strains YC20(T) and XD15 was 98.2 % and these sequences showed 89.6-92.8 % similarity to those of the most closely related members of the genus Halobellus. The DNA G+C contents of strains YC20(T) and XD15 were 65.8 mol% and 65.4 mol%, respectively. The DNA-DNA hybridization value between strain YC20(T) and strain XD15 was 92 %, and the two strains showed low DNA-DNA relatedness to members of the genus Halobellus. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains YC20(T) and XD15 represent a novel species of the genus Halobellus, for which the name Halobellus inordinatus sp. nov. is proposed. The type strain is YC20(T) ( = CGMCC 1.12120(T) = JCM 18361(T)) and the other strain is XD15 ( = CGMCC 1.12236 = JCM 18648).


Assuntos
Halobacteriaceae/classificação , Lagos/microbiologia , Filogenia , Microbiologia da Água , Composição de Bases , China , DNA Arqueal/genética , Genes Arqueais , Glicolipídeos/química , Halobacteriaceae/genética , Halobacteriaceae/isolamento & purificação , Cloreto de Magnésio , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Sais , Cloreto de Sódio , Temperatura
11.
Int J Syst Evol Microbiol ; 63(Pt 10): 3915-3919, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24097833

RESUMO

Members of the haloarchaeal genera Halosarcina and Halogeometricum (family Halobacteriaceae) are closely related to each other and show 96.6-98 % 16S rRNA gene sequence similarity. This is higher than the accepted threshold value (95 %) to separate two genera, and a taxonomic study using a polyphasic approach of all four members of the two genera was conducted to clarify their relationships. Polar lipid profiles indicated that Halogeometricum rufum RO1-4(T), Halosarcina pallida BZ256(T) and Halosarcina limi RO1-6(T) are related more to each other than to Halogeometricum borinquense CGMCC 1.6168(T). Phylogenetic analyses using the sequences of three different genes (16S rRNA gene, rpoB' and EF-2) strongly supported the monophyly of these four species, showing that they formed a distinct clade, separate from the related genera Halopelagius, Halobellus, Haloquadratum, Haloferax and Halogranum. The results indicate that the four species should be assigned to the same genus, and it is proposed that Halosarcina pallida and Halosarcina limi be transferred to the genus Halogeometricum as Halogeometricum pallidum comb. nov. (type strain, BZ256(T) = KCTC 4017(T) = JCM 14848(T)) and Halogeometricum limi comb. nov. (type strain, RO1-6(T) = CGMCC 1.8711(T) = JCM 16054(T)).


Assuntos
Halobacteriaceae/classificação , Filogenia , DNA Arqueal/genética , Genes Arqueais , Halobacteriaceae/genética , Lipídeos/análise , Dados de Sequência Molecular , Fator 2 de Elongação de Peptídeos/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
12.
Antonie Van Leeuwenhoek ; 104(5): 885-91, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23949820

RESUMO

Two halophilic archaeal strains, YC87(T) and YCA11, were isolated from Yuncheng salt lake in Shanxi, China. Cells of the two strains were observed to be pleomorphic rod-shaped, stained Gram-negative and produced red-pigmented colonies. Strain YC87(T) was able to grow at 20-50 °C (optimum 37 °C), at 1.4-4.8 M NaCl (optimum 2.1 M NaCl), at 0.05-1.0 M MgCl2 (optimum 0.3 M MgCl2) and at pH 6.0-9.0 (optimum pH 7.0) while strain YCA11 was able to grow at 20-50 °C (optimum 37 °C), at 2.1-4.8 M NaCl (optimum 3.1 M NaCl), at 0.01-0.7 M MgCl2 (optimum 0.1 M MgCl2) and at pH 6.0-9.0 (optimum pH 7.5). The cells of both isolates were observed to lyse in distilled water. The minimum NaCl concentrations that prevented cell lysis were determined to be 8 % (w/v) for strain YC87(T) and 12 % (w/v) for strain YCA11. The major polar lipids of the two strains were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether; another major glycolipid and trace amounts of several unidentified lipids were also detected. The 16S rRNA gene sequences of the two strains were 99.8 % identical, showing 93.2-98.2 % similarity to members of the genus Halorubrum of the family Halobacteriaceae. The rpoB' gene similarity between strains YC87(T) and YCA11 was 99.3 % and showed 87.5-95.2 % similarity to the closest relative members of the genus Halorubrum. The DNA G+C content of strains YC87(T) and YCA11 were determined to be 64.9 and 64.5 mol%, respectively. The DNA-DNA hybridization value between strain YC20(T) and strain YC77 was 87 % and the two strains showed low DNA-DNA relatedness with Halorubrum cibi JCM 15757(T) and Halorubrum aquaticum CGMCC 1.6377(T), the most related members of the genus Halorubrum. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains YC87(T) and YCA11 represent a novel species of the genus Halorubrum, for which the name Halorubrum rubrum sp. nov. is proposed. The type strain is YC87(T) (=CGMCC 1.12124(T) = JCM 18365(T)).


Assuntos
Halorubrum/classificação , Halorubrum/isolamento & purificação , Lagos/microbiologia , Composição de Bases , China , Análise por Conglomerados , Meios de Cultura/química , DNA Arqueal/química , DNA Arqueal/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Glicolipídeos/metabolismo , Halorubrum/genética , Halorubrum/fisiologia , Humanos , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Fosfolipídeos/análise , Filogenia , Pigmentos Biológicos/metabolismo , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Cloreto de Sódio/metabolismo
13.
Antonie Van Leeuwenhoek ; 104(3): 377-84, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23828176

RESUMO

Two halophilic archaeal strains, YC21(T) and YC77, were isolated from an inland salt lake of China. Both have pleomorphic rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require at least 2.1 M NaCl for growth under the optimum growth temperature of 37 °C. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS), two major glycolipids (GL1 and GL2) chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified lipids (GL0-1 and GL0-2) were also detected. The 16S rRNA gene sequences of the two strains are 99.9 % identical, show 94.0-98.9 % similarity to the closest relative members of Halobellus of the family Halobacteriaceae. The rpoB' gene similarity between strains YC21(T) and YC77 is 99.8 % and show 90.3-95.3 % similarity to the closest relative members of Halobellus. The DNA G+C content of strains YC21(T) and YC77 were 66.1 and 66.2 mol%, respectively. The DNA-DNA hybridization value between strain YC20(T) and strain YC77 was 89 %, and the two strains showed low DNA-DNA relatedness with Halobellus limi TBN53(T), the most related member of Halobellus. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains YC21(T) and YC77 represent a novel species of the genus Halobellus, for which the name Halobellus rarus sp. nov. is proposed. The type strain is YC21(T) (=CGMCC 1.12121(T) = JCM 18362(T)).


Assuntos
Halobacteriaceae/classificação , Halobacteriaceae/isolamento & purificação , Lagos/microbiologia , Composição de Bases , China , Análise por Conglomerados , DNA Arqueal/química , DNA Arqueal/genética , DNA Ribossômico/química , DNA Ribossômico/genética , RNA Polimerases Dirigidas por DNA/genética , Glicolipídeos/análise , Halobacteriaceae/genética , Halobacteriaceae/fisiologia , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Fosfolipídeos/análise , Filogenia , Pigmentos Biológicos/metabolismo , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Cloreto de Sódio/metabolismo , Temperatura
14.
Sci Rep ; 13(1): 19446, 2023 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-37945754

RESUMO

This study investigates the relationships among government intervention, industrial structure, and energy eco-efficiency (EE). Energy eco-efficiency was measured based on a non-radial directional distance function for 236 cities in China from 2005 to 2019. Additionally, the difference-in-difference model (DID) method and spatial econometric models were used to analyse the impact of government intervention and industrial structure on energy eco-efficiency and their spatial spill-over effects. Government intervention includes fiscal expenditures and policy orientation for new energy demonstration construction. Our results indicate that: China's EE has a fluctuating upward trend and increased 17.85% in the period, and its spatial distribution imbalance gradually developed into a regional distribution balance. Moreover, government intervention and adjustment of the industrial structure improved urban energy eco-efficiency by 7.43% and 0.92%, respectively, which also has spatial spill-over effects in neighbouring regions. Furthermore, economic development, technological innovation, and foreign direct investment enable EE. However, urbanisation hinders the improvement of energy eco-efficiency. Finally, heterogeneity analysis showed that the policy of the new energy demonstration city has better effects on eastern and western cities in promoting EE.

15.
Extremophiles ; 16(6): 895-901, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23052830

RESUMO

Two halophilic archaeal strains, R30(T) and tADL(T), were isolated from an aquaculture farm in Dailing, China, and from Deep Lake, Antarctica, respectively. Both have rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require >120 g/l NaCl and 48-67 g/l MgCl(2) for growth but differ in their optimum growth temperatures (30 °C, tADL(T) vs. 40 °C, R30(T)). The major polar lipids were typical for members of the Archaea but also included a major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The 16S rRNA gene sequences of the two strains are 97.4 % identical, show most similarity to genes of the family Halobacteriaceae, and cluster together as a distinct clade in phylogenetic tree reconstructions. The rpoB' gene similarity between strains R30(T) and tADL(T) is 92.9 % and less to other halobacteria. Their DNA G + C contents are 62.4-62.9 mol % but DNA-DNA hybridization gives a relatedness of only 44 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe two new species of a novel genus, represented by strain R30(T) (= CGMCC 1.10593(T) = JCM 17270(T)) and strain tADL(T) (= JCM 15066(T) = DSMZ 22187(T)) for which we propose the names Halohasta litorea gen. nov., sp. nov. and Halohasta litchfieldiae sp. nov., respectively.


Assuntos
Halobacteriaceae/classificação , Halobacteriaceae/isolamento & purificação , Regiões Antárticas , Proteínas Arqueais/genética , China , DNA Arqueal/química , Halobacteriaceae/citologia , Filogenia , RNA Ribossômico 16S/genética
16.
Sheng Wu Gong Cheng Xue Bao ; 37(12): 4455-4464, 2021 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-34984890

RESUMO

According to the teaching philosophy of the outcome-based education, this study elaborates the development of a practical innovation course for biological engineering major after five runs of teaching practice and continuous improvement. It mainly includes the methods for selection of teaching subjects, implementation of teaching process, process assessment, evaluation and improvement. Based on the performance and achievements of three grades of students majored in bioengineering, we found that the logic and methods of the practical innovation course could greatly stimulate the motivation of students for learning, as well as their scores. Therefore, the logic and methods described in this study may serve as a reference for the reforms of practical training courses of engineering major under the background of Engineering Education Certification.


Assuntos
Currículo , Estudantes , Bioengenharia , Certificação , Humanos , Aprendizagem
17.
J Periodontal Implant Sci ; 51(3): 147-162, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34114379

RESUMO

PURPOSE: This systematic review and meta-analysis was conducted to assess the effects of glycine powder air-polishing (GPAP) in patients during supportive periodontal therapy (SPT) compared to hand instrumentation and ultrasonic scaling. METHODS: The authors searched for randomized clinical trials in 8 electronic databases for relevant studies through November 15, 2019. The eligibility criteria were as follows: population, patients with chronic periodontitis undergoing SPT; intervention and comparison, patients treated by GPAP with a standard/nozzle type jet or mechanical instrumentation; and outcomes, bleeding on probing (BOP), patient discomfort/pain (assessed by a visual analogue scale [VAS]), probing depth (PD), gingival recession (Rec), plaque index (PI), clinical attachment level (CAL), gingival epithelium score, and subgingival bacteria count. After extracting the data and assessing the risk of bias, the authors performed the meta-analysis. RESULTS: In total, 17 studies were included in this study. The difference of means for BOP in patients who received GPAP was lower (difference of means: -8.02%; 95% confidence interval [CI], -12.10% to -3.95%; P<0.00001; I²=10%) than that in patients treated with hand instrumentation. The results of patient discomfort/pain measured by a VAS (difference of means: -1.48, 95% CI, -1.90 to -1.06; P<0.001; I²=83%) indicated that treatment with GPAP might be less painful than ultrasonic scaling. The results of PD, Rec, PI, and CAL showed that GPAP had no advantage over hand instrumentation or ultrasonic scaling. CONCLUSIONS: The findings of this study suggest that GPAP may alleviate gingival inflammation more effectively and be less painful than traditional methods, which makes it a promising alternative for dental clinical use. With regards to PD, Rec, PI, and CAL, there was insufficient evidence to support a difference among GPAP, hand instrumentation, and ultrasonic scaling. Higher-quality studies are still needed to assess the effects of GPAP.

18.
Bioresour Technol ; 282: 433-438, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30889534

RESUMO

The aim of this work was to realize 1,2,4-butantriol (BT) production from sugarcane bagasse hydrolysates by microbial fermentation, and obtain co-production of BT and ethanol. Candida glycerinogenes UG21 was utilized to reduce the effect of osmolality resulting from high glucose concentration and furfural in hydrolysates on cell growth of BT-producing strains, and produced 54.1 g/L ethanol from glucose. After ethanol recovering, xylose containing stillage was obtained and used for BT production. 1.3 g/L BT was generated by a BT-producing strain. By the deletion of the crr gene and process optimization, BT titer reached 4.9 g/L. Meanwhile, the efficient utilization of sugarcane bagasse was achieved by a two-stage fermentation for co-production of BT and ethanol. This study provided a novel strategy for BT production from sugarcane bagasse, and demonstrated the potential for making full use of sugarcane bagasse hydrolysates to co-production value-added products.


Assuntos
Butanóis/metabolismo , Candida/metabolismo , Etanol/metabolismo , Lignina/metabolismo , Celulose/química , Fermentação , Saccharum/química
19.
Bioresour Technol ; 273: 634-640, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30502643

RESUMO

The aim of this work was to study ethanol fermentation properties of the robust mutant Candida glycerinogenes UG21 from non-detoxified lignocellulose hydrolysate. C. glycerinogenes UG21 with high tolerance to elevated temperature, acetic acid, and furfural was obtained and applied in lignocellulose-based ethanol production. C. glycerinogenes UG21 exhibited highly-efficient degradation ability to furfural. High levels of acetic acid and furfural inhibited cell growths and ethanol production of Saccharomyces cerevisiae ZWA46 and industrial Angel yeast but had a slight impact on biomass and ethanol titer of C. glycerinogenes UG21. Using non-detoxified sugarcane bagasse hydrolysate, C. glycerinogenes UG21 reached 1.24 g/L/h of ethanol productivity at 40 °C but ethanol production of S. cerevisiae ZWA46 and Angel yeast was inhibited. Further, C. glycerinogenes UG-21 exhibited 2.42-fold and 1.58-fold higher productivity than S. cerevisiae ZWA46 and Angel yeast under low-toxicity hydrolysate. Therefore, C. glycerinogenes UG-21 could be an excellent candidate for low-cost lignocelluloses ethanol production.


Assuntos
Candida/metabolismo , Etanol/metabolismo , Fermentação , Lignina/metabolismo , Ácido Acético/metabolismo , Biomassa , Celulose/metabolismo , Furaldeído/metabolismo , Hidrólise , Saccharomyces cerevisiae/metabolismo , Saccharum/metabolismo
20.
Mol Clin Oncol ; 2(3): 449-453, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24772316

RESUMO

Erlotinib and pemetrexed have been approved for the second-line treatment of non-small cell lung cancer. Recent reports indicated that erlotinib and pemetrexed exerted synergistic effects against lung adenocarcinoma. The available treatment options for lung cancer with brain metastases (BM) are currently limited. In the present study, we investigated the efficacy of the combined administration of erlotinib and pemetrexed in 9 patients with epidermal growth factor receptor (EGFR) wild-type lung adenocarcinoma with BM. Pemetrexed (500 mg/m2) and cisplatin (20 mg/m2) were administered on day 1 and days 1-3, respectively. Erlotinib (150 mg) was administered daily on days 4-20. The 9 patients harbored EGFR wild-type mutation in the primary tumor tissues. With regard to the BM, no patients achieved complete remission, 7 patients exhibited a partial response (PR), 1 had stable disease (SD) and 1 had progressive disease (PD). As regards the extracranial tumors, 3 patients exhibited a PR, 2 had SD, 3 had PD and 1 was not applicable. The performance status and the symptoms improved in 3 patients following treatment. The median progression-free survival for intracranial and extracranial disease control was 179 and 146.5 days, respectively. The median overall survival was 197.4 days. Therefore, erlotinib combined with pemetrexed/cisplatin, was found to be effective in the treatment of patients with EGFR wild-type lung adenocarcinoma.

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