RESUMO
BACKGROUND: Men who have sex with men (MSM) are at high risk of HIV and sexually transmitted infections (STIs) in China and globally. Engaging in commercial sex put them at even greater risk. This study estimated the prevalence of HIV/STIs among three subgroups of MSM: MSM who sold sex (MSM-selling), MSM who bought sex (MSM-buying), and non-commercial MSM (NC-MSM) and evaluated the relationship between commercial sex and HIV/STIs. METHODS: We conducted a cross-sectional survey among MSM in six Chinese cities (Shenyang, Ji'nan, Changsha, Zhengzhou, Nanjing, and Kunming) from 2012 to 2013. Data on socio-demographics and sexual behaviors were collected. Serological tests were conducted to detect HIV, syphilis, and human simplex virus type 2 (HSV-2). RESULTS: Of 3717 MSM, 6.8% were engaged in commercial sex. The overall prevalence of HIV, syphilis and HSV-2 infections was 11.1, 8.8 and 12.1%, respectively. MSM-selling had higher prevalence of HIV (13.4%), syphilis (12.1%) and HSV-2 (17.9%) than NC-MSM (10.9, 8.7 and 11.9% for HIV, syphilis and HSV-2, respectively), though the differences are not statistically significant. Among MSM-selling, HIV prevalence was significantly higher for those who found sex partners via Internet than those did not (19.4% vs. 8.1%, P = 0.04). Compared to NC-MSM, MSM-selling were more likely to use recreation drugs (59.3% vs. 26.3%), have unprotected anal intercourse (77.9% vs. 61.7%), and have ≥10 male sex partners (46.2% vs. 6.2%) in the past 6 months (each P < 0.05). CONCLUSIONS: All three subgroups of MSM in six large Chinese cities have high prevalence of HIV/STIs. Those who sell sex only have a particularly high risk of acquiring and transmitting disease, and therefore, they should be considered as a priority group in HIV/STIs surveillance and intervention programs.
Assuntos
Infecções por HIV/epidemiologia , Herpes Genital/epidemiologia , Homossexualidade Masculina/estatística & dados numéricos , Trabalho Sexual/estatística & dados numéricos , Sífilis/epidemiologia , Adulto , China/epidemiologia , Cidades/epidemiologia , Estudos Transversais , Herpesvirus Humano 2/patogenicidade , Humanos , Masculino , Fatores de Risco , Parceiros Sexuais , Infecções Sexualmente Transmissíveis/epidemiologia , Adulto JovemRESUMO
Porphyromonas gingivalis is a Gram-negative obligately anaerobic bacterium associated with several forms of periodontal disease, most closely with chronic periodontitis. Previous studies demonstrated that OxyR plays an important role in the aerotolerance of P. gingivalis by upregulating the expression of oxidative-stress genes. Increases in oxygen tension and in H(2)O(2) both induce activation of OxyR. It is also known that P. gingivalis requires hemin as an iron source for its growth. In this study, we found that a hemin-limited growth environment significantly enhanced OxyR activity in P. gingivalis. As a result, expression of sod, dps, and ahpC was also upregulated. Using a chromatin immunoprecipitation quantitative PCR (qPCR) analysis, DNA binding of activated OxyR to the promoter of the sod gene was enhanced in P. gingivalis grown under hemin-limited conditions compared to excess-hemin conditions. Cellular tolerance of H(2)O(2) was also enhanced when hemin was limited in the growth medium of P. gingivalis. Our work supports a model in which hemin serves as a signal for the regulation of OxyR activity and indicates that P. gingivalis coordinately regulates expression of oxidative-stress-related genes by this hemin concentration-dependent pathway.
Assuntos
Hemina/fisiologia , Porphyromonas gingivalis/metabolismo , Fatores de Transcrição/metabolismo , Meios de Cultura , DNA/metabolismo , Ensaio de Imunoadsorção Enzimática , Regulação Bacteriana da Expressão Gênica/fisiologia , Ferro/química , Ferro/metabolismo , Mutação , Estresse Oxidativo , Reação em Cadeia da Polimerase , Porphyromonas gingivalis/genética , Ligação Proteica , RNA Bacteriano/genética , RNA Bacteriano/metabolismo , Fatores de Transcrição/genéticaRESUMO
OBJECTIVE: The objective of this study was to use the newly synthesized poly(quaternary ammonium salt) (PQAS)-containing polyacid to formulate the light-curable glass-ionomer cements and study the effect of the PQAS on the compressive strength and antibacterial activity of the formed cements. MATERIALS AND METHODS: The functional QAS and their constructed PQAS were synthesized, characterized and formulated into the experimental high-strength cements. Compressive strength (CS) and Streptococcus mutans viability were used to evaluate the mechanical strength and antibacterial activity of the cements. Fuji II LC cement was used as control. The specimens were conditioned in distilled water at 37°C for 24 h prior to testing. The effects of the substitute chain length, loading as well as grafting ratio of the QAS and aging on CS and S. mutans viability were investigated. RESULTS: All the PQAS-containing cements showed a significant antibacterial activity, accompanying with an initial CS reduction. The effects of the chain length, loading and grafting ratio of the QAS were significant. Increasing chain length, loading, grafting ratio significantly enhanced antibacterial activity but reduced the initial CS. Under the same substitute chain length, the cements containing QAS bromide were found to be more antibacterial than those containing QAS chloride although the CS values of the cements were not statistically different from each other, suggesting that we can use QAS bromide directly without converting bromide to chloride. The experimental cement showed less CS reduction and higher antibacterial activity than Fuji II LC. The long-term aging study suggests that the cements may have a long-lasting antibacterial function. CONCLUSIONS: This study developed a novel antibacterial glass-ionomer cement. Within the limitations of this study, it appears that the experimental cement is a clinically attractive dental restorative due to its high mechanical strength and antibacterial function.