Dehydrative alkynylation of 3-hydroxyisoindolinones with terminal alkynes for the synthesis of 3-alkynylated 3,3-disubstituted isoindolinones.

A brand-new procedure for the synthesis of 3-alkynylated 3,3-disubstituted isoindolinones has been disclosed via a HOTf or Fe(OTf)3-catalyzed dehydrative alkynylation of 3-hydroxyisoindolinones with terminal alkynes. Aryl, alkenyl and alkyl terminal alkynes are suitable to couple with a broad range of 3-hydroxyisoindolinones to afford the desired products in moderate to good yields. This protocol features the use of an inexpensive catalyst, mild reaction conditions, broad substrate scope and easy elaboration of the products.

Tandem phospha-Michael addition/cyclization/dehydration of 2-hydroxychalcones with H-phosphine oxides for the synthesis of 4-phosphorylated 4H-chromenes.

A Hg(OTf)2-catalyzed tandem phospha-Michael addition/cyclization/dehydration of 2-hydroxychalcones with H-phosphine oxides is presented. This protocol provides a new and supplementary approach for the preparation of 4-phosphorylated 4H-chromenes in good yields (up to 99%). In addition, this domino reaction allows the successful construction of two new C-P and C-O bonds in a one-pot operation.

Copper(II)-catalyzed direct dehydrative alkynylation of 2H-chromene hemiketals with terminal alkynes to 2,2-disubstituted 2-alkynylated 2H-chromenes.

The first copper-catalyzed direct dehydrative alkynylation of 2H-chromene hemiketals with terminal alkynes has been uncovered. The use of cheap and readily available CuCl2 as the catalyst allowed the preparation of various 2,2-disubstituted 2-alkynylated 2H-chromenes in moderate to good yields, which compensates for the limitation of the current methods only suited for the synthesis of 2-monosubsituted 2-alkynylated 2H-chromenes.

Synthesis of 2H-chromenes: recent advances and perspectives.

As one type of chromene, 2H-chromenes represent important oxygen heterocycles, which not only widely exist in natural products, pharmaceutical agents and biologically relevant molecules, but have also been used broadly in materials science and organic synthesis. Two major synthetic strategies have been developed towards such compounds. This review mainly focuses on the recent advances in this field, including benzopyran ring formation involving cyclization reactions and the late-stage functionalization of the parent 2H-chromenes.

Y(OTf)3-catalyzed phosphorylation of 2H-chromene hemiacetals with P(O)-H compounds to 2-phosphorylated 2H-chromenes.

A facile synthesis of 2-phosphorylated 2H-chromenes has been accomplished herein via a Y(OTf)3-catalyzed dehydrative coupling of 2H-chromene hemiacetals with P(O)-H compounds. This protocol features low catalyst loading, mild reaction conditions, broad substrate scope and easy elaboration of the products.

Intraspecific leaf trait variation mediates edge effects on litter decomposition rate in fragmented forests.

There is strong trait dependence in species-level responses to environmental change and their cascading effects on ecosystem functioning. However, there is little understanding of whether intraspecific trait variation (ITV) can also be an important mechanism mediating environmental effects on ecosystem functioning. This is surprising, given that global change processes such as habitat fragmentation and the creation of forest edges drive strong trait shifts within species. On 20 islands in the Thousand Island Lake, China, we quantified intraspecific leaf trait shifts of a widely distributed shrub species, Vaccinium carlesii, in response to habitat fragmentation. Using a reciprocal transplant decomposition experiment between forest edge and interior on 11 islands with varying areas, we disentangled the relative effects of intraspecific leaf trait variation versus altered environmental conditions on leaf decomposition rates in forest fragments. We found strong intraspecific variation in leaf traits in response to edge effects, with a shift toward recalcitrant leaves with low specific leaf area and high leaf dry matter content from forest interior to the edge. Using structural equation modeling, we showed that such intraspecific leaf trait response to habitat fragmentation had translated into significant plant afterlife effects on leaf decomposition, leading to decreased leaf decomposition rates from the forest interior to the edge. Importantly, the effects of intraspecific leaf trait variation were additive to and stronger than the effects from local environmental changes due to edge effects and habitat loss. Our experiment provides the first quantitative study showing that intraspecific leaf trait response to edge effects is an important driver of the decrease in leaf decomposition rate in fragmented forests. By extending the trait-based response-effect framework toward the individual level, intraspecific variation in leaf economics traits can provide the missing functional link between environmental change and ecological processes. These findings suggest an important area for future research on incorporating ITV to understand and predict changes in ecosystem functioning in the context of global change.

Sparse large trees in secondary and planted forests highlight the need to improve forest conservation and management.

Large trees are essential for carbon storage and biodiversity conservation. While an increasing number of studies have focused on large trees in primary forests, little is known about them in secondary and planted forests. We surveyed 86,936 trees in secondary forests and 91,294 trees in planted forests in Zhejiang, China, to investigate the distribution patterns and determinants of large trees in these forests. We found a mean density of large trees (DBH ≥ 30 cm) of 15 ± 13 stems ha-1 in secondary forests and 11 ± 9 stems ha-1 in planted forests. Moreover, the mean density of trees with DBH ≥ 60 cm was 0.36 stems ha-1, indicating that large trees are particularly rare in secondary and planted forests. These large trees were primarily occurred in secondary forests that living in high-elevation area with less human exploitation and colder and wetter climates, and in planted forests with higher species richness and lower tree density. In addition, the density of large trees in these forests significantly increased with tree species richness and decreased with increasing tree density. These results indicate that the sparse large trees were the legacy of historical human activities in the studied area, but currently, the development of large trees is still limited by the improper forest structure characterized by low species diversity and high tree density. To better conserve large trees, there is an urgent need for enhanced conservation policies for secondary forests, such as establishing forest parks for forests with large trees, and implementing near-natural forest management practices for planted forests, which include planting mixed native tree species and maintaining moderate tree density.

Size-focused conservation may fail to protect the world's oldest trees.

Old trees are irreplaceable natural resources that provide multifaceted benefits to humans. Current conservation strategies focus primarily on large-sized trees that were often considered old. However, some studies have demonstrated that small trees can be more than thousands of years old, suggesting that conventional size-focused perceptions may hamper the efficiency of current conservation strategies for old trees. Here, we compiled paired age and diameter data using tree-ring records sampled from 121,918 trees from 269 species around the world to detect whether tree size is a strong predictor of age for old trees and whether the spatial distribution of small old trees differs from that of large old trees. We found that tree size was a weak predictor of age for old trees, and diameter explained only 10% of the total age variance of old trees. Unlike large-sized trees that are mainly in warm, wet environments and protected, small old trees are predominantly in cold, dry environments and mostly unprotected, indicating that size-focused conservation failed to protect some of the oldest trees. To conserve old trees, comprehensive old-tree recognition systems are needed that consider not only tree size but also age and external characteristics. Protected areas designed for small old trees are urgently needed.

Increases in intraspecific body size variation are common among North American mammals and birds between 1880 and 2020.

Many studies have documented the average body size of animals declining over time. Compared to mean body size, less is known about long-term changes in intraspecific trait variation (ITV), which is also important to understanding species' ability to cope with environmental challenges. On the basis of 393,499 specimen records from 380 species collected in North America between 1880 and 2020, we found that body size ITV increased by 9.59% for mammals (n = 302) and 30.67% for birds (n = 78); human-harvested species had higher probability of ITV increase. The observed increasing ITV in many species suggests possible niche expansion and potential buffering effects against downsizing but it risks increased maladaptation to rapidly changing environments. The results demonstrate that trait mean and variance do not necessarily respond in similar ways to anthropogenic pressures and both should be considered.

Disentangling biotic and abiotic drivers of intraspecific trait variation in woody plant seedlings at forest edges.

In fragmented forests, edge effects can drive intraspecific variation in seedling performance that influences forest regeneration and plant composition. However, few studies have attempted to disentangle the relative biotic and abiotic drivers of intraspecific variation in seedling performance. In this study, we carried out a seedling transplant experiment with a factorial experimental design on three land-bridge islands in the Thousand Island Lake, China, using four common native woody plant species. At different distances from the forest edge (2, 8, 32, 128 m), we transplanted four seedlings of each species into each of three cages: full-cage, for herbivore exclusion; half-cage, that allowed herbivore access but controlled for caging artifacts; and no-cage control. In the 576 cages, we recorded branch architecture, leaf traits, and seedling survival for each seedling before and after the experimental treatment. Overall, after one full growing season, edge-induced abiotic drivers and varied herbivory pressure led to intraspecific variation in seedling performance, including trade-offs in seedling architecture and resource-use strategies. However, responses varied across species with different life-history strategies and depended on the driver in question, such that the abiotic and biotic effects were additive across species, rather than interactive. Edge-induced abiotic variation modified seedling architecture of a shade-tolerant species, leading to more vertical rather than lateral growth at edges. Meanwhile, increased herbivory pressure resulted in a shift toward lower dry matter investment in leaves of a light-demanding species. Our results suggest that edge effects can drive rapid directional shifts in the performance and intraspecific traits of some woody plants from early ontogenetic stages, but most species in this study showed negligible phenotypic responses to edge effects. Moreover, species-specific responses suggest the importance of interspecific differences modulating the degree of trait plasticity, implying the need to incorporate individual-level responses when understanding the impact of forest fragmentation on plant communities.