OFDR shape sensor based on a femtosecond-laser-inscribed weak fiber Bragg grating array in a multicore fiber.

An optical frequency domain reflectometry (OFDR) shape sensor was demonstrated based on a femtosecond-laser-inscribed weak fiber Bragg grating (WFBG) array in a multicore fiber (MCF). A WFBG array consisting of 60 identical WFBGs was successfully inscribed in each core along a 60 cm long MCF using the femtosecond-laser point-by-point technology, where the length and space of each WFBG were 2 and 8 mm, respectively. The strain distribution of each core in two-dimensional (2D) and three-dimensional (3D) shape sensing was successfully demodulated using the traditional cross correlation algorithm, attributed to the accurate localization of each WFBG. The minimum reconstruction error per unit length of the 2D and 3D shape sensors has been improved to 1.08% and 1.07%, respectively, using the apparent curvature vector method based on the Bishop frame.

High-spatial-resolution φ-OFDR shape sensor based on multicore optical fiber with femtosecond-laser-induced permanent scatter arrays: publisher's note.

This publisher's note contains a correction to Opt. Lett.48, 3219 (2023)10.1364/OL.486644.

Ultra-linear broadband optical frequency sweep for a long-range and centimeter-spatial-resolution OFDR.

We demonstrated a long-range and centimeter-spatial-resolution optical frequency domain reflectometry (OFDR) system based on an ultra-linear broadband optical frequency sweep. The high nonlinear sweeping effect of the distributed feedback (DFB) diode laser was suppressed by a pre-distortion method, ensuring that the injection-locking process remained stable during fast tuning over a large span. An optical linear frequency sweep (LFS) with a sweep range and sweep rate of up to 60 GHz and 15 THz/s, respectively, was ultimately obtained by optimizing the injection-locking system. The high performance OFDR based on the proposed LFS achieved a sampling spatial resolution of 1.71 mm. Furthermore, distributed strain sensing was implemented with high-spatial resolutions of about 5 cm and 7 cm in the measurement range over 1 km and 2 km, respectively.

High-spatial-resolution φ-OFDR shape sensor based on multicore optical fiber with femtosecond-laser-induced permanent scatter arrays.

An optical fiber φ-OFDR shape sensor with a submillimeter spatial resolution of 200 µm was demonstrated by using femtosecond-laser-induced permanent scatter array (PS array) multicore fiber (MCF). A PS array was successfully inscribed in each slightly twisted core of the 400-mm-long MCF. The two-dimensional (2D) and three-dimensional (3D) shapes of the PS-array-inscribed MCF were successfully reconstructed by using PS-assisted φ-OFDR, vector projections, and the Bishop frame based on the PS-array-inscribed MCF. The minimum reconstruction error per unit length of the 2D and 3D shape sensor was 2.21% and 1.45%, respectively.

Wide-range OFDR strain sensor based on the femtosecond-laser-inscribed weak fiber Bragg grating array.

A wide-range OFDR strain sensor was demonstrated based on femtosecond-laser-inscribed weak fiber Bragg grating (WFBG) array in standard SMF. A WFBG array consisting of 110 identical WFBGs was successfully fabricated along a 56 cm-long SMF. Compared with SMF, the cross-correlation coefficient of WFBG array was improved to 0.9 under the strain of 10,000 µÎµ. The position deviation under the strain of 10,000 µÎµ, i.e., 2.5 mm, could be accurately obtained and compensated simply by using peak finding algorithm. The maximum measurable strain of single- and multi-point strain sensing was up to 10,000 µÎµ without using any additional algorithms, where the sensing spatial resolution was 5 mm.

Distributed Refractive Index Sensing Based on Etched Ge-Doped SMF in Optical Frequency Domain Reflectometry.

A distributed optical fiber refractive index sensor based on etched Ge-doped SMF in optical frequency domain reflection (OFDR) was proposed and demonstrated. The etched Ge-doped SMF was obtained by only using wet-etching, i.e., hydrofluoric acid solution. The distributed refractive index sensing is achieved by measuring the spectral shift of the local RBS spectra using OFDR. The sensing length of 10 cm and the spatial resolution of 5.25 mm are achieved in the experiment. The refractive index sensing range is as wide as 1.33-1.44 refractive index units (RIU), where the average sensitivity was about 757 GHz/RIU. Moreover, the maximum sensitivity of 2396.9 GHZ/RIU is obtained between 1.43 and 1.44 RIU.

Distributed high-temperature sensing based on optical frequency domain reflectometry with a standard single-mode fiber.

Distributed temperature sensing up to 600°C at a fiber length of 100.75 m based on optical frequency domain reflectometry (OFDR) was demonstrated using a standard single-mode fiber (SMF) without any treatment. The spatial resolution was 2.5 mm. An algorithm, instantaneous optical frequency resampling (IOFR), to eliminate the nonlinearity of the laser source was proposed and used to obtain calibrated reference and measurement signals that were used for temperature demodulation. Moreover, the temperature response stability of the annealed SMF was better than that of un-annealed SMF, where the temperature sensitivity was 1.96 GHz/°C at 600°C.

Submillimeter-spatial-resolution φ-OFDR strain sensor using femtosecond laser induced permanent scatters.

A φ-optical frequency domain reflectometry (OFDR) strain sensor with a submillimeter-spatial-resolution of 233 µm is demonstrated by using femtosecond laser induced permanent scatters (PSs) in a standard single-mode fiber (SMF). The PSs-inscribed SMF, i.e., strain sensor, with an interval of 233 µm exhibited a Rayleigh backscattering intensity (RBS) enhancement of 26 dB and insertion loss of 0.6 dB. A novel, to the best of our knowledge, method, i.e., PSs-assisted φ-OFDR, was proposed to demodulate the strain distribution based on the extracted phase difference of P- and S-polarized RBS signal. The maximum measurable strain was up to 1400 µÎµ at a spatial resolution of 233 µm.

High-quality fiber Bragg grating inscribed in ZBLAN fiber using femtosecond laser point-by-point technology.

We demonstrate for the first time, to the best of our knowledge, the fabrication of a high-quality fiber Bragg grating (FBG) in ZBLAN fiber by using an efficient femtosecond laser point-by-point technology. Two types of FBG, e.g., high coupling coefficient and narrow bandwidth grating, are successfully obtained. The coupling coefficient is strongly dependent on the grating order and pulse energy. A second-order FBG with an ultrahigh coupling coefficient of 325 m-1 and reflectivity of 97.8% is inscribed in the ZBLAN fiber. A pair of FBGs with a narrow FWHM of 0.30 and 0.09 nm are also demonstrated.

High-Spatial-Resolution OFDR Distributed Temperature Sensor Based on Step-by-Step and Image Wavelet Denoising Methods.

A high-spatial-resolution OFDR distributed temperature sensor based on Au-SMF was experimentally demonstrated by using step-by-step and image wavelet denoising methods (IWDM). The measured temperature between 50 and 600 °C could be successfully demodulated by using SM-IWDM at a spatial resolution of 3.2 mm. The temperature sensitivity coefficient of the Au-SMF was 3.18 GHz/°C. The accuracy of the demodulated temperature was approximately 0.24 °C. Such a method has great potential to expand the temperature measurement range, which is very useful for high-temperature applications.

A Nondestructive Measurement Method of Optical Fiber Young's Modulus Based on OFDR.

A nondestructive measurement method based on an Optical frequency domain reflectometry (OFDR) was demonstrated to achieve Young's modulus of an optical fiber. Such a method can be used to measure, not only the averaged Young's modulus within the measured fiber length, but also Young's modulus distribution along the optical fiber axis. Moreover, the standard deviation of the measured Young's modulus is calculated to analyze the measurement error. Young's modulus distribution of the coated and uncoated single mode fiber (SMF) samples was successfully measured along the optical fiber axis. The average Young's modulus of the coated and uncoated SMF samples was 13.75 ± 0.14, and 71.63 ± 0.43 Gpa, respectively, within the measured fiber length of 500 mm. The measured Young's modulus distribution along the optical fiber axis could be used to analyze the damage degree of the fiber, which is very useful to nondestructively estimate the service life of optical fiber sensors immersed into smart engineer structures.

Chondroitin synthase-3 regulates nucleus pulposus degeneration through actin-induced YAP signaling.

Loss of chondroitin sulfate (CS) has been reported to play a key role during intervertebral disc degeneration (IDD). However, the detailed mechanism of CS and its synthases have not been elucidated. Since CS is mainly synthesized by chondroitin synthases 3 (Chsy3), here, the Chsy3 knockout mice are generated by using CRISPR-Cas9 and semi-cloning technology to study its mechanism during IDD. We find that CS and Chsy3 expression are decreased during IDD both in human and mice nucleus pulposus (NP) tissue, and knockout of Chsy3 shows that spontaneous IDD phenotype resembles that of human samples in the Chsy3-/- mice. Taking advantage of RNA-Seq data, we confirm increased catabolic and decreased anabolic changes in Chsy3-/- NP cells. By using bioinformatic analysis and validation, we find that Hippo signaling pathway is significantly downregulated, and the activation of Yap1 is mainly affected in Chsy3-/- NP cells. Furthermore, functional analyses have shown that Chsy3 could regulate NP cell degeneration by Actin tension mediated activation of Yap1, which is independent of Hippo/Lats signaling. In summary, our findings reveal a novel mechanism that depletion of CS-related Chsy3 can cause spontaneous intervertebral disc degeneration by mediating Yap activation through CS-related actin-tension in NP cells.

Inflammatory-sensitive CHI3L1 protects nucleus pulposus via AKT3 signaling during intervertebral disc degeneration.

Intervertebral disc degeneration (IDD) is the main cause of low back pain and the mechanism of which is far from fully revealed. Although inflammation directed nucleus pulposus (NP) extracellular matrix metabolism dysregulation is known to be the main cause of the degeneration process, few is known about the protective factors. Using high-throughput label-free proteomics, we found that inflammation-related autocrine factor Chitinase-3-like protein 1 (CHI3L1, or YKL-40) is highly expressed in the NP cells during degeneration. Immunohistochemical analysis show that the expression of CHI3L1 is NP tissue specific, and increase significantly during degeneration. Overexpression of CHI3L1 significantly decrease the catabolism, and increase the anabolism of extracellular matrix. Knockdown of CHI3L1 using siRNAs show the opposite results, which imply that the protective role of CHI3L1 in IDD. Using high-throughput RNA sequencing and functional analyses, we find that AKT3 expression and its phosphorylation is mainly regulated by CHI3L1. And lastly, the mechanism of which is also validated using human and mouse degenerated NP tissues. In summary, our findings show that the inflammation-related autocrine factor CHI3L1 is NP specific, and it protects IDD by promoting the AKT3 signaling, which may serve as a potential therapeutic target in intervertebral disc degeneration.

Role of HSP90α in osteoclast formation and osteoporosis development.

Osteoporosis (OP) is a systemic metabolic bone disease that occurs most frequently in the elderly. The main pathogenesis of OP is excessive proliferation and differentiation of osteoclasts, in which the peroxisome proliferator-activated receptor γ (PPARγ) pathway has a pivotal role. Recently, heat shock protein (HSP)90α has been identified as an important molecular chaperone with PPARγ, which regulates the effect of the PPARγ pathway. The aim of the present study was to investigate the role of HSP90α involved in the regulation of osteoclast formation and the process of osteoporosis. Firstly, the expression of HSP90α in osteoclast differentiation was detected by western blotting in vitro, then the effect of HSP90α inhibition on the formation and differentiation of osteoclasts was examined. Furthermore, the nuclear import of PPARγ was also assessed to confirm the synergistic effect of HSP90α. Finally, the inhibitory effect of HSP90α in vivo was explored, using a mouse model of osteoporosis. As a result, in the process of osteoclast differentiation and proliferation, the expression of HSP90α was upregulated. Inhibition of HSP90α could block the formation and differentiation of osteoclasts, and remit osteoporosis in mice. Regarding the underlying mechanism, inhibition of HSP90α could block the nuclear import of PPARγ to inhibit osteoclast differentiation and proliferation. In conclusion, these data indicated that the inhibition of HSP90α could block osteoclast formation and remit osteoporosis by reducing the nuclear import of PPARγ.

Quasi-Distributed Temperature and Strain Sensors Based on Series-Integrated Fiber Bragg Gratings.

Two types of series-integrated fiber Bragg gratings (SI-FBGs), i.e., strong and weak SI-FBGs, were inscribed in a standard single-mode fiber (SMF) using the femtosecond laser point-by-point technology. In the SI-FBGs inscribing system, the grating pitch of each FBG and the distance between the two adjacent FBGs in the SI-FBGs can be flexibly controlled by adjusting the inscription parameters. The strong SI-FBGs with different grating pitches and the weak SI-FBGs with an identical grating pitch were employed to successfully measure the temperature distribution in a tube furnace and the strain distribution on a cantilever beam, respectively. A high spatial resolution of less than 1 mm was achieved during the distributed temperature sensing experiment. Moreover, the spatial resolution could be improved by decreasing the distance between the two adjacent FBGs, i.e., decreasing the FBG length and the space between the two adjacent FBGs. Hence, the inscribed high-quality SI-FBGs have great potential to be developed as various quasi-distributed sensors with a high spatial resolution.

Anterior cervical discectomy and fusion, open-door laminoplasty, or laminectomy with fusion: Which is the better treatment for four-level cervical spondylotic myelopathy?

Four-level cervical spondylotic myelopathy (CSM) is a common disease affecting a large number of people, with the optimal surgical strategy remaining controversial. This study compared the clinical outcomes, radiological parameters, and postoperative complications of primarily performed surgical procedures such as anterior cervical discectomy and fusion (ACDF), open-door laminoplasty (LAMP), and laminectomy with fusion (LF) in treating four-level CSM. A total of 116 patients who received ACDF (38 cases), LAMP (45 cases), and LF (33 cases) were followed up for a minimum of 24 months were enrolled in this study and retrospectively analyzed. Clinical outcomes were evaluated using the Japanese Orthopedic Association (JOA) scoring system, the Neck Disability Index (NDI), and the Visual Analogue Scale (VAS). Changes in the curvature of the cervical spine were determined using the cervical curvature index (CCI) and the C2-C7 Cobb angle. Cervical mobility was evaluated using the C2-C7 range of motion (ROM) and active cervical ROM (aROM). Complications were recorded and compared among the three groups. All patients achieved significant improvement in JOA, NDI, and VAS scores at the final follow-up (P < 0.05), whereas no remarkable difference was found among the groups (P > 0.05). In addition, both C2-7 ROM and aROM were significantly reduced in the three groups and LAMP showed the least reduction relatively. As for complications, LAMP showed the lowest overall incidence of postoperative complications, and patients in the ACDF group were more susceptible to dysphagia, pseudoarthrosis than LAMP and LF. Considering improvements in clinical symptoms and neurological function, no remarkable difference was found among the groups. Nevertheless, LAMP had advantages over the other two surgical procedures in terms of preserving cervical mobility and reducing postoperative complications.

Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1.

Ossification of the posterior longitudinal ligament (OPLL) is an emerging spinal disease caused by heterotopic ossification of the posterior longitudinal ligament. The pathological mechanism is poorly understood, which hinders the development of nonsurgical treatments. Here, we set out to explore the function and mechanism of small extracellular vesicles (sEVs) in OPLL. Global miRNA sequencings are performed on sEVs derived from ligament cells of normal and OPLL patients, and we have showed that miR-320e is abundantly expressed in OPLL-derived sEVs compare to other sEVs. Treatment with either sEVs or miR-320e significantly promote the osteoblastic differentiation of normal longitudinal ligament cells and mesenchymal stem cells and inhibit the osteoclastic differentiation of monocytes. Through a mechanistic study, we find that TAK1 is a downstream target of miR-320e, and we further validate these findings in vivo using OPLL model mice. Together, our data demonstrate that OPLL ligament cells secrete ossification-promoting sEVs that contribute to the development of ossification through the miR-320e/TAK1 axis.

Intervertebral-spreader-assisted anterior cervical discectomy and fusion prevents postoperative axial pain by alleviating facet joint pressure.

OBJECTIVE: To evaluate the relationship of postoperative cervical axial pain with different vertebral distraction methods used during ACDF procedures in cervical spondylosis patients. METHODS: Ninety-four single-level cervical spondylotic myelopathy patients with significantly loss of intervertebral disc height who underwent ACDF surgery in our institute between January 2018 and January 2020 were enrolled. Cervical spine lateral radiographs were taken preoperatively, 3 days, 1-month, 2-month and 6-month after the surgery. The intervertebral disc height (IDH), interfacet distance (IFD), JOA (Japanese Orthopaedic Association) score, NDI (Neck Disability Index) score, nVAS (Neck Visual Analogue Scale) score and aVAS (Arm Visual Analogue Scale) score were measured. The correlation of clinical parameters and intervertebral disc height was evaluated. Then the correlation of clinical outcomes and different distraction method was evaluated. The patients were randomly divided into two groups, one uses Casper pin distractor system alone for distraction (Caspar alone group) and the other uses spreader assisted distraction method (Casper + spreader group). In biomechanical study, four cervical spine cadavers were selected for facet pressure measurements under different vertebral distraction methods, and the facet joint pressure was measured using force sensors. RESULTS: Satisfactory cervical fusion and neurological recovery were achieved in all patients. No significant correlation of IDH, IFD, JOA, NDI or aVAS with nVAS score was found. No significant difference between the change in disc height and clinical outcomes was found. However, by comparing the clinical parameters of patients in different vertebral distraction groups, we found significant changes in the early nVAS and NDI scores (P = 0.11, P = 0.48) of the Casper + spreader group (3 days postoperation), and was associated with a better nVAS score at 2 months postoperation (P < 0.05). The biomechanical study in cervical cadavers also showed significantly and continuously decreased facet joint pressure in the spreader assisted vertebral distraction group (P < 0.01). CONCLUSIONS: Spreader-assisted vertebral distraction method effectively alleviates postoperative neck pain in degenerative cervical spondylosis patients treated with ACDF. The mechanism may be related to the transient relief of facet joint pressure during the vertebral distraction procedure in ACDF.

MicroRNA-181 regulates the development of Ossification of Posterior longitudinal ligament via Epigenetic Modulation by targeting PBX1.

Objectives: Ossification of the posterior longitudinal ligament (OPLL) presents as the development of heterotopic ossification in the posterior longitudinal ligament of the spine. The etiology of OPLL is genetically linked, as shown by its high prevalence in Asian populations. However, the molecular mechanism of the disease remains obscure. In this study, we explored the function and mechanism of OPLL-specific microRNAs. Methods: The expression levels of the ossification-related OPLL-specific miR-181 family were measured in normal or OPLL ligament tissues. The effect of miR-181a on the ossification of normal or pathogenic ligament cells was tested using real-time polymerase chain reaction (PCR), Western blot, alizarin red staining and alkaline phosphatase (ALP) staining. The candidate targets of miR-181 were screened using a dual luciferase reporter assay and functional analysis. The link between miR-181a and its target PBX1 was investigated using chromatin immunoprecipitation, followed by real-time PCR detection. Histological and immunohistochemical analysis as well as micro-CT scanning were used to evaluate the effects of miR-181 and its antagonist using both tip-toe-walking OPLL mice and in vivo bone formation assays. Results: Using bioinformatic analysis, we found that miR-181a-5p is predicted to play important roles in the development of OPLL. Overexpression of miR-181a-5p significantly increased the expression of ossification-related genes, staining level of alizarin red and ALP activity, while the inhibition of miR-181a-5p by treatment with an antagomir had the opposite effects. Functional analysis identified PBX1 as a direct target of miR-181a-5p, and we determined that PBX1 was responsible for miR-181a-5p's osteogenic phenotype. By chromatin immunoprecipitation assay, we found that miR-181a-5p controls ligament cell ossification by regulating PBX1-mediated modulation of histone methylation and acetylation levels in the promoter region of osteogenesis-related genes. Additionally, using an in vivo model, we confirmed that miR-181a-5p can substantially increase the bone formation ability of posterior ligament cells and cause increased osteophyte formation in the cervical spine of tip-toe-walking mice. Conclusions: Our data unveiled the mechanism by which the miR-181a-5p/PBX1 axis functions in the development of OPLL, and it revealed the therapeutic effects of the miR-181a-5p antagomir in preventing OPLL development both in vivo and in vitro. Our work is the first to demonstrate that microRNA perturbation could modulate the development of OPLL through epigenetic regulation.