RESUMO
Bulk matrix stiffness has emerged as a key mechanical cue in stem cell differentiation. Here, we show that the commitment and differentiation of human mesenchymal stem cells encapsulated in physiologically soft (â¼0.2-0.4 kPa), fully synthetic polyisocyanopeptide-based three-dimensional (3D) matrices that mimic the stiffness of adult stem cell niches and show biopolymer-like stress stiffening, can be readily switched from adipogenesis to osteogenesis by changing only the onset of stress stiffening. This mechanical behaviour can be tuned by simply altering the material's polymer length whilst maintaining stiffness and ligand density. Our findings introduce stress stiffening as an important parameter that governs stem cell fate in a 3D microenvironment, and reveal a correlation between the onset of stiffening and the expression of the microtubule-associated protein DCAMKL1, thus implicating DCAMKL1 in a stress-stiffening-mediated, mechanotransduction pathway that involves microtubule dynamics in stem cell osteogenesis.
Assuntos
Hidrogéis , Células-Tronco Mesenquimais/fisiologia , Estresse Mecânico , Materiais Biocompatíveis , Fenômenos Biomecânicos , Técnicas de Cultura de Células , Diferenciação Celular , Quinases Semelhantes a Duplacortina , Regulação da Expressão Gênica/fisiologia , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Teste de Materiais , Estrutura Molecular , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismoRESUMO
The differentiation of stem cells can be modulated by physical factors such as the micro- and nano-topography of the extracellular matrix. One important goal in stem cell research is to understand the concept that directs differentiation into a specific cell lineage in the nanoscale environment. Here, we demonstrate that such paths exist by controlling only the micro- and nano-topography of polymer surfaces. Altering the depth (on a nanometric scale) of micro-patterned surface structures allowed increased adhesion of human mesenchymal stem cells (hMSCs) with specific differentiation into osteoblasts, in the absence of osteogenic medium. Small (10 nm) depth patterns promoted cell adhesion without noticeable differentiation, whereas larger depth patterns (100 nm) elicited a collective cell organization, which induced selective differentiation into osteoblast-like cells. This latter response was dictated by stress through focal-adhesion-induced reorganization of F-actin filaments. The results have significant implications for understanding the architectural effects of the in vivo microenvironment and also for the therapeutic use of stem cells.
Assuntos
Diferenciação Celular , Extensões da Superfície Celular/fisiologia , Matriz Extracelular/fisiologia , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/fisiologia , Actinas/metabolismo , Adesão Celular , Células Cultivadas , Adesões Focais/fisiologia , Humanos , Microscopia Eletrônica de Varredura , Nanoestruturas , Osteoblastos/citologia , Osteoblastos/fisiologia , OsteogêneseRESUMO
The interior of the cell nucleus is comparable to a solid network bathed in an interstitial fluid. From the extrapolation of low frequency data, it is expected that such network should dictate the response of the nucleus to mechanical stress at high frequencies, described by unique elastic moduli. However, none of the existing techniques that can probe the mechanical properties of cells can exceed the kHz range, and the mechanics of the nuclear network remain poorly understood. We use laser-generated acoustic waves to probe remotely the stiffness and viscosity of nuclei in single cells in the previously unexplored GHz range with a â¼100 nm axial resolution. The probing of cells at contrasted differentiation stages, ranging from stem cells to mature cells originating from different tissues, demonstrates that the mechanical properties of the nuclear network are common across various cell types. This points to an asymptotically increasing influence of a solid meshwork of connected chromatin fibers.
Assuntos
Diferenciação Celular , Cromatina/química , Ondas de Choque de Alta Energia , Linhagem Celular Tumoral , Cromatina/metabolismo , HumanosRESUMO
We present a method of preparing and characterizing nanostructured bioactive motifs using a combination of nanoimprint lithography and surface functionalization. Nanodots were fabricated on silicon surfaces and modified with a cell-adhesive RGD peptide for studies in human mesenchymal stem cell adhesion and differentiation. We report that bioactive nanostructures induce mature focal adhesions on human mesenchymal stem cells with an impact on their behavior and dynamics specifically in terms of cell spreading, cell-material contact, and cell differentiation.
Assuntos
Células-Tronco Mesenquimais/química , Nanoestruturas/química , Oligopeptídeos/química , Adesão Celular , Diferenciação Celular , Humanos , Células-Tronco Mesenquimais/citologia , Propriedades de SuperfícieRESUMO
An experimental model is introduced for the induction of endothelial cell (EC) tubulogenesis after 24 h of incubation on micropatterned polymer surfaces. Pericytes or mesenchymal stem cells are added separately to this system to evaluate their effect on tubular stabilization. In the absence of additional cells, the tubular structures are lost after 36 h. Addition of only pericytes, however, stabilizes the EC vasculogenic tubes.
Assuntos
Células-Tronco Mesenquimais/citologia , Pericitos/citologia , Animais , Células Cultivadas , Humanos , Neovascularização Fisiológica/fisiologiaRESUMO
How endothelial cells (ECs) express the particular filopodial or lamellipodial form of the actin machinery is critical to understanding EC functions such as angiogenesis and sprouting. It is not known how these mechanisms coordinately promote lumen formation of ECs. Here, adhesion molecules (RGD peptides) and inductor molecules (BMP-2 mimetic peptides) are micropatterned onto polymer surfaces by a photolithographic technique to induce filopodial and lamellipodial migration modes. Firstly, the effects of peptide microgeometrical distribution on EC adhesion, orientation and morphogenesis are evaluated. Large micropatterns (100 µm) promote EC orientation without lumen formation, whereas small micropatterns (10-50 µm) elicit a collective cell organization and induce EC lumen formation, in the case of RGD peptides. Secondly, the correlation between EC actin machinery expression and EC self-assembly into lumen formation is addressed. Only the filopodial migration mode (mimicked by RGD) but not lamellipodial migration mode (mimicked by BMP-2) promotes EC lumen formation. This work gives a new concept for the design of biomaterials for tissue engineering and may provide new insight for angiogenesis inhibition on tumors.
Assuntos
Actinas/metabolismo , Células Endoteliais/citologia , Células Endoteliais/metabolismo , Adesão Celular/fisiologia , Movimento Celular/fisiologia , Células Cultivadas , Células Endoteliais/fisiologia , Células Endoteliais da Veia Umbilical Humana/citologia , Células Endoteliais da Veia Umbilical Humana/metabolismo , Células Endoteliais da Veia Umbilical Humana/fisiologia , Humanos , Neovascularização Fisiológica/fisiologia , Pseudópodes/metabolismo , Pseudópodes/fisiologiaRESUMO
This work reports on the synthesis of titanium bone implants functionalized with nanoparticles (NPs) containing Arg-Gly-Asp-Cys peptide (RGDC) and shows the adhesion behavior of cells seeded on these materials. RGDC peptides were first conjugated to a norbornenyl-poly(ethylene oxide) macromonomer (Nb-PEO). Then, functional NPs with a size of â¼300 nm and constituted of polynorbornene core surrounded by poly(ethylene oxide) shell were prepared by ring-opening metathesis polymerization in dispersed medium. The grafting density of these NPs on the titanium surface is up to 2 NPs·µm(-2) (80 pmol of RGDC per cm(-2) of NP surface). Cell adhesion was evaluated using preosteoblast cells (MC3T3-E1). Results of cells cultured for 24 h showed that materials grafted with NPs functionalized with RGDC peptides enhance specific cell adhesion and can create filopodia-like structures among NP sites by stressing the cells.
Assuntos
Adesão Celular , Materiais Revestidos Biocompatíveis/química , Nanopartículas , Oligopeptídeos/química , Osteoblastos/metabolismo , Titânio/química , Animais , Células Cultivadas , Materiais Revestidos Biocompatíveis/metabolismo , Teste de Materiais , Camundongos , Osteoblastos/citologia , Polietilenoglicóis/química , Próteses e Implantes , Crânio/citologia , Crânio/metabolismo , Propriedades de SuperfícieRESUMO
The mechanical properties of cells play a key role in several fundamental biological processes, such as migration, proliferation, differentiation and tissue morphogenesis. The complexity of the inner cell composition and the intricate meshwork formed by transmembrane cell-substrate interactions demands a non-invasive technique to probe cell mechanics and cell adhesion at a subcell scale. In this paper we review the use of laser-generated GHz acoustic waves--a technique called picosecond ultrasonics (PU)--to probe the mechanical properties of single cells. We first describe applications to vegetal cells and biomimetic systems. We show how these systems can be used as simple models to understand more complex animal cells. We then present an opto-acoustic bio-transducer designed for in vivo measurements in physiological conditions. We illustrate the use of this transducer through the simultaneous probing of the density and compressibility of Allium cepa cells. Finally, we demonstrate that this technique can quantify animal-cell adhesion on metallic surfaces by analyzing the acoustic pulses reflected off the cell-metal interface. This innovative approach allows investigating quantitatively cell mechanics without fluorescent labels or mechanical contact to the cell.
Assuntos
Adesão Celular/fisiologia , Análise de Célula Única/métodos , Ultrassom/métodos , Allium/citologia , Animais , Fenômenos Fisiológicos Celulares , Microscopia Acústica , Monócitos/citologia , TransdutoresRESUMO
Physicochemical features of a cell nanoenvironment exert important influence on stem cell behavior and include the influence of matrix elasticity and topography on differentiation processes. The presence of growth factors such as TGF-ß and BMPs on these matrices provides chemical cues and thus plays vital role in directing eventual stem cell fate. Engineering of functional biomimetic scaffolds that present programmed spatio-temporal physical and chemical signals to stem cells holds great promise in stem cell therapy. Progress in this field requires tacit understanding of the mechanistic aspects of cell-environment nanointeractions, so that they can be manipulated and exploited for the design of sophisticated next generation biomaterials. In this review, we report and discuss the evolution of these processes and pathways in the context of matrix adhesion as they might relate to stemness and stem cell differentiation. Super-resolution microscopy and single-molecule methods for in vitro nano-manipulation are helping to identify and characterize the molecules and mechanics of structural transitions within stem cells and matrices. All these advances facilitate research toward understanding of stem cell niche and consequently to developing new class of biomaterials helping the "used biomaterials" for applications in tissue engineering and regenerative medicine.
Assuntos
Materiais Biocompatíveis/química , Fenômenos Químicos , Nanoestruturas/química , Células-Tronco/citologia , Diferenciação Celular/efeitos dos fármacos , Humanos , Nicho de Células-Tronco , Transplante de Células-Tronco , Especificidade por Substrato , Engenharia Tecidual , Alicerces TeciduaisRESUMO
We report the characterization of three-dimensional membrane waves for migrating single and collective cells and describe their propagation using wide-field optical profiling technique with nanometer resolution. We reveal the existence of small and large membrane waves the amplitudes of which are in the range of â¼ 3-7 nm to â¼ 16-25 nm respectively, through the cell. For migrating single-cells, the amplitude of these waves is about 30 nm near the cell edge. Two or more different directions of propagation of the membrane nanowaves inside the same cell can be observed. After increasing the migration velocity by BMP-2 treatment, only one wave direction of propagation exists with an increase in the average amplitude (more than 80 nm near the cell edge). Furthermore for collective-cell migration, these membrane nanowaves are attenuated on the leader cells and poor transmission of these nanowaves to follower cells was observed. After BMP-2 treatment, the membrane nanowaves are transmitted from the leader cell to several rows of follower cells. Surprisingly, the vast majority of the observed membrane nanowaves is shared between the adjacent cells. These results give a new view on how single and collective-cells modulate their motility. This work has significant implications for the therapeutic use of BMPs for the regeneration of skin tissue.
Assuntos
Membrana Celular/fisiologia , Movimento Celular/fisiologia , Animais , Proteína Morfogenética Óssea 2/metabolismo , Linhagem Celular , Imageamento Tridimensional , Camundongos , Microscopia/métodosRESUMO
The reflection of picosecond ultrasonic pulses from a cell-substrate interface is used to probe cell-biomaterial adhesion with a subcell resolution. We culture monocytes on top of a thin biocompatible Ti metal film, supported by a transparent sapphire substrate. Low-energy femtosecond pump laser pulses are focused at the bottom of the Ti film to a micron spot. The subsequent ultrafast thermal expansion launches a longitudinal acoustic pulse in Ti, with a broad spectrum extending up to 100 GHz. We measure the acoustic echoes reflected from the Ti-cell interface through the transient optical reflectance changes. The time-frequency analysis of the reflected acoustic pulses gives access to a map of the cell acoustic impedance Zc and to a map of the film-cell interfacial stiffness K simultaneously. Variations in Zc across the cell are attributed to rigidity and density fluctuations within the cell, whereas variations in K are related to interfacial intermolecular forces and to the nano-architecture of the transmembrane bonds.
Assuntos
Fenômenos Ópticos , Análise de Célula Única/métodos , Titânio/química , Ultrassom/métodos , Adesão Celular , Monócitos/citologia , Propriedades de Superfície , TemperaturaRESUMO
Stem cells cultured on extracellular matrix (ECM) with different stiffnesses have been shown to engage into different lineage commitments. However, in vivo, the components of the ECM are known to bind and strongly interact with growth factors. The effect, on the stem cell fate, of the cooperation between the mechanical properties and the growth factor in the same microenvironment has not yet been investigated. Here, we propose a protocol for mimicking this stem cell microenvironment with an in vitro system. This system consists in grafting (without using a spacer) biomolecules that contain N-termini groups onto hydrogel (poly(acrylamide-co-acrylic acid)) surfaces of various stiffnesses ranging from 0.5 to 70 kPa. First, we demonstrate that the commitment of mesenchymal stem cell populations changes in response to the substrate's rigidity, with myogenic differentiation occurring at 13-17 kPa and osteogenic differentiation at 45-49 kPa. Chemical grafting of soft and stiff matrices with an osteogenic factor (BMP-2(mimetic peptide)) results only in osteogenic differentiation. Also, when grafted on even softer gels (0.5-3.5 kPa), the BMP-2(mimetic peptide) had no effect on the stem cell differentiation. We prove that correct organization of F-actin cytoskeleton due to the mechanical properties of the microenvironment is necessary for BMP-induced smad1/5/8 phosphorylation and nuclear translocation. These results suggest that stem cell differentiation is dictated mechanically, but in the presence of a biochemical factor, the effect of the mechanical factor on stem cell commitment is modified. This can explain the diversity of stem cell behaviors in vivo where different growth factors are sequestrated on the ECM.
Assuntos
Proteína Morfogenética Óssea 2/farmacologia , Hidrogéis/química , Células-Tronco Mesenquimais/citologia , Osteogênese/fisiologia , Acrilamidas/análise , Acrilamidas/química , Actinas/metabolismo , Materiais Biocompatíveis/química , Adesão Celular/fisiologia , Diferenciação Celular/fisiologia , Células Cultivadas , Citoesqueleto/metabolismo , Matriz Extracelular/química , Humanos , Ligantes , Microscopia de Fluorescência , Espectroscopia FotoeletrônicaRESUMO
Mature osteoblasts are the cells responsible for bone formation and are derived from precursor osteoblasts. However, the mechanisms that control this differentiation are poorly understood. In fact, unlike the majority of organs in the body, which are composed of "soft" tissue from which cells can easily be isolated and studied, the "hard" mineralized tissue of bone has made it difficult to study the function of bone cells. Here, we established an in vitro model that mimics this differentiation under physiological conditions. We obtained mature osteoblasts and characterized them on the basis of the following parameters: the strong expression of osteoblastic markers, such as Runx2 and Col-I; the achievement of specific dimensions (the cell volume increases 26-fold compared to the osteoblast precursors); and the production of an abundant extracellular matrix also called osteoid. We demonstrated that the differentiation of osteoblast precursors into mature osteoblasts requires the continuous activation of Bone Morphogenetic Protein (BMP) receptors, which we established with the immobilization of a BMP-2mimetic peptide on a synthetic matrix mimicking in vivo microenvironment. Importantly, we demonstrated that the organization of the F-actin network and acetylated microtubules of the cells were modified during the differentiation process. We showed that the perturbation of the F-actin cytoskeleton organization abolished the differentiation process. In addition, we demonstrated that expression of the Runx2 gene is required for this differentiation. These findings demonstrate the retro-regulation of cytoplasmic and genic components due to the continuous induction of BMP-2 and also provide more detailed insights into the correct signaling of BMPs for cell differentiation in bone tissue.
RESUMO
Microenvironments such as protein composition, physical features, geometry, and elasticity play important roles in stem cell lineage specification. The components of the extracellular matrix are known to subsequently assemble into fibrillar networks in vivo with defined periodicity. However, the effect of the most critical parameter, which involves the periodicity of these fibrillar networks, on the stem cell fate is not yet investigated. Here, we show the effect of synthetic fibrillar networks patterned with nanometric periodicities, using bottom-up approaches, on the response of stem cells. We have used helical organic nanoribbons based on self-assemblies of Gemini-type amphiphiles to access chiral silica nanoribbons with two different shapes and periodicities (twisted ribbons and helical ribbons) from the same native self-assembled organic nanostructure. We demonstrate the covalent grafting of these silica nanoribbons onto activated glass substrates and the influence of this programmed isotropically oriented matrix to direct the commitment of human mesenchymal stem cells (hMSCs) into osteoblast lineage in vitro, free of osteogenic-inducing media. The specific periodicity of 63 nm (±5 nm) with helical ribbon shape induces specific cell adhesion through the fibrillar focal adhesion formation and leads to stem cell commitment into osteoblast lineage. In contrast, the matrix of periodicity 100 nm (±15 nm) with twisted ribbon shape does not lead to osteoblast commitment. The inhibition of non-muscle myosin II with blebbistatin is sufficient to block this osteoblast commitment on helical nanoribbon matrix, demonstrating that stem cells interpret the nanohelical shape and periodicity environment physically. These results indicate that hMSCs could interpret nanohelical shape and periodicity in the same way they sense microenvironment elasticity. This provides a promising tool to promote hMSC osteogenic capacity, which can be exploited in a 3D scaffold for bone tissue engineering.
Assuntos
Materiais Biocompatíveis/química , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/fisiologia , Nanopartículas/química , Osteoblastos/citologia , Osteoblastos/fisiologia , Osteogênese/fisiologia , Diferenciação Celular , Sobrevivência Celular , Células Cultivadas , Humanos , Nanopartículas/ultraestrutura , Propriedades de Superfície , Engenharia Tecidual/métodosRESUMO
Angiogenesis, the formation of new blood vessels by sprouting from pre-existing ones, is critical for the establishment and maintenance of complex tissues. Angiogenesis is usually triggered by soluble growth factors such as VEGF. However, geometrical cues also play an important role in this process. Here we report the induction of angiogenesis solely by SVVYGLR peptide micropatterning on polymer surfaces. SVVYGLR peptide stripes were micropatterned onto polymer surfaces by photolithography to study their effects on endothelial cell (EC) behavior. Our results showed that the EC behaviors (cell spreading, orientation and migration) were significantly more guided and regulated on narrower SVVYGLR micropatterns (10 and 50 µm) than on larger stripes (100 µm). Also, EC morphogenesis into tube formation was switched on onto the smaller patterns. We illustrated that the central lumen of tubular structures can be formed by only one-to-four cells due to geometrical constraints on the micropatterns which mediated cell-substrate adhesion and generated a correct maturation of adherens junctions. In addition, sprouting of ECs and vascular networks were also induced by geometrical cues on surfaces micropatterned with SVVYGLR peptides. These micropatterned surfaces provide opportunities for mimicking angiogenesis by peptide modification instead of exogenous growth factors. The organization of ECs into tubular structures and the induction of sprouting angiogenesis are important towards the fabrication of vascularized tissues, and this work has great potential applications in tissue engineering and tissue regeneration.
Assuntos
Células Endoteliais da Veia Umbilical Humana/citologia , Neovascularização Fisiológica/fisiologia , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/fisiologia , Células Cultivadas , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Humanos , Neovascularização Fisiológica/efeitos dos fármacos , Peptídeos/farmacologia , Polímeros/farmacologiaRESUMO
The bone morphogenetic proteins (BMPs) are cytokines of the transforming growth factor beta family. Some BMPs such as BMP-2, BMP-7 and BMP-9 play a major role in the bone and cartilage formation. The BMP peptides corresponding to residues 73-92, 89-117, and 68-87 of BMP-2, BMP-7 and BMP-9 respectively as well as adhesion peptides (GRGDSPC) were grafted onto polyethylene terephthatalate (PET) surfaces. We evaluated the state of differentiation of pre-osteoblastic cells. The behavior of these cells on various functionalized surfaces highlighted the activity of the mimetic peptides immobilized on surfaces. The induced cells (observed in the case of surfaces grafted with BMP-2, 7 or 9 mimetic peptides and GRGDSPC peptides) were characterized on several levels. First of all, we focused on the evaluation of the osteoblastic markers such as the transcriptional factor Runx2, which is a critical regulator of osteoblastic differentiation. Secondly, the results obtained showed that these induced cells take a different morphology compared to the cells in a state of proliferation or in a state of extracellular matrix production. Induced cells were characterized by an increased thickness compared to non-induced cells. Thus, our studies prove a direct correlation between cell morphology and state of induction. Thereafter, we focused on characterizing the extracellular matrix formed by the cells on various surfaces. The extracellular matrix thickness was more significant in the case of surfaces grafted with mimetic peptides of the BMP-2, 7 or 9 and GRGDSPC peptides which once again proves their activity when immobilized on material surface. These results demonstrate that GRGDSPC and BMPs peptides, grafted to PET surface, act to enhance osteogenic differentiation and mineralization of pre-osteoblastic cells. These findings are potentially useful in developing engineered biomaterials for bone regeneration.