RESUMO
The process of cell division is critical to the growth and development of an organism. As a fertilized egg develops into a mature organism, tissues undergo cellular renewal or commit to terminal differentiation and leave the cell cycle. Tight regulation of events controlling the cell cycle ensures the integrity of the genetic information and prevents aberrant or unscheduled cell division [...].
Assuntos
Proteínas de Ciclo Celular/genética , Ciclo Celular/genética , Diferenciação Celular/genética , Pontos de Checagem do Ciclo Celular/genética , Divisão Celular/genética , Humanos , Células-Tronco/citologiaRESUMO
This work describes the SDS-CGE of proteins carried out in microchannels made of the negative photoresist EPON SU-8. Embedded electrophoretic microchannels have been fabricated with a multilayer technology based on bonding and releasing steps of stacked SU-8 films. This technology allows the monolithic integration of the electrodes in the device. A high wafer fabrication yield and mass production compatibility guarantees low costs and high reliability. A poly(methyl methacrylate) (PMMA) packaging allows an easy setup and replacement of the device for electrophoresis experiments. In addition, the wire-bonding step is avoided. The electrophoretic mobilities of four proteins have been measured in microchannels filled with polyacrylamide. Different pore sizes have been tested obtaining their Ferguson plots. Finally, a separation of two proteins (20 and 36 kDa) has been carried out confirming that this novel device is suitable for protein separation. A resolution of 2.75 is obtained. This is the first time that this SU-8 microfluidic technology has been validated for SDS-CGE of proteins. This technology offers better separation performance than glass channels, at lower costs and with an easy packaging procedure.