[2-Methyl-3-phenylaminomethylquinolin-4-one as potential antidepressant with nootropic properties].

The new compound, 2-methyl-3-phenylaminomethylquinolin-4-on, belongs to V class of toxicity and exhibits antidepressant and antiamnesic properties. It is established that this compound reduces the duration of immobilization in the test of behavioral despair, prevents from the scopolamine induced amnesia, and exhibits antagonism with reserpine in mice. In a dose of 100 mg/kg, the synthesized compound influences the levels of cerebral catecholamines similarly to imipramine, but with a more pronounced decrease in the level of 5-hydroxytryptamine.

Structure of a phosphorylated polysaccharide from Shewanella putrefaciens strain S29.

A phosphorylated polysaccharide was isolated from the aqueous layer of the phenol-water extract of a non-halophilic bacterium Shewanella putrefaciens strain S29. The glycosyl phosphate linkage in the polysaccharide was split under mild acid conditions to give, after borohydride reduction, a phosphorylated oligosaccharide-alditol. On the basis of sugar analysis and 1H, 13C and 31P NMR spectroscopy, including 2D COSY, relayed COSY, rotating-frame NOE spectroscopy (ROESY), heteronuclear 13C,1H COSY, and H-detected heteronuclear 1H,31P multiple-quantum coherence (HMQC), it was concluded that the polysaccharide is built up of tetrasaccharide-phosphate repeating units having the following structure: [sequence: see text] where QuiNAc and Qui4NAc are 2-acetamido-2,6-dideoxyglucose and 4-acetamido-4,6-dideoxyglucose, respectively.

Structure of the capsular polysaccharide from Alteromonas sp. CMM 155.

Capsular polysaccharide (CPS) was obtained by water-saline extraction of the Alteromonas sp. CMM 155. On the basis of solvolysis with anhydrous HF and 1H- and 13C-NMR spectral data, including NOE experiments, it was concluded that the capsular polysaccharide had the following structure containing novel N-acyl-amino sugar and bacillosamine residues: --> 3)-alpha-D-GalpNAc-(1 --> 4)-alpha-L-GalApNAc(1 --> 3)- alpha-D-QuipNAc4NAc-(1 --> 3)-beta-D-Quip4NAlaAc-(1 -->

Structure of the O-specific polysaccharide from Pseudoalteromonas elyakovii sp. nov. CMM 162.

The structure of the O-specific polysaccharide from lipopolysaccharide of Pseudoalteromonas elyakovii sp. nov. CMM 162 on the basis of NMR data, Smith degradation and methylation study was elucidated as follows:-->2)-alpha-D-Glcp-(1-->4)-beta-D-GalpNAc-(1--> 3)-alpha-D-Galp-(1-->3)-beta-D-Galp-NAc-(1-->6)-alpha-D-Glcp-(1-->.

Structure of the acidic polysaccharide chain of the lipopolysaccharide of Shewanella alga 48055.

A lipopolysaccharide (LPS) with an acidic polysaccharide chain was isolated from the bacterium Shewanella alga strain 48055 and cleaved selectively at the glycosidic linkage of N-acetylneuraminic acid to give a tetrasaccharide. Studies of the tetrasaccharide and the O-deacylated LPS by 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, rotating-frame NOE spectroscopy (ROESY), and H-detected 1H, 13C heteronuclear multiple-quantum coherence (HMQC) experiments, revealed the following structure of the polysaccharide repeating unit: -->3)-beta-D-GalpA6GroN-(1-->3)-beta-D-GlcpNAc-(1-->3)-alpha-D- GalpA6GroN- (1-->4)-alpha-Neup5Ac-(2--> where GroN is an amidically linked residue of 2-amino-1,3-propanediol (2-amino-2-deoxyglycerol). A similar structure, but with 2-acetamido-2,6-dideoxy-D-glucose instead of 2-acetamido-2-deoxy-D-glucose, has been reported previously for the polysaccharide chain of a non-O1 Vibrio cholerae H11 LPS [E. V. Vinogradov, O. Holst, J.E. Thomas-Oates, K.W. Broady, and H. Brade, Eur. J. Biochem., 210 (1992) 491-498].

Structure of the capsular polysaccharide from Alteromonas nigrifaciens IAM 13010T containing 2-acetamido-2,6-dideoxy-L-talose and 3-deoxy-D-manno-octulosonic acid.

A capsular polysaccharide was obtained from Alteromonas nigrifaciens IAM 13010T by saline extraction. On the basis of 1H and 13C NMR spectroscopy, including one-dimensional (1D) NOE spectroscopy, 2D rotating-frame NOE spectroscopy (ROESY), and 1H-detected heteronuclear 1H,13C multiple-quantum coherence (HMQC), it was concluded that the polysaccharide contained inter alia an acidic sugar, 3-deoxy-D-manno-octulosonic acid (Kdo), and a rare amino sugar, 2-acetamido-2,6-dideoxy-L-talose (L-6dTalNAc, N-acetylpneumosamine), and has a pentasaccharide repeating unit of the following structure: [equation: see text]

Structure of a colitose-containing O-specific polysaccharide of the marine bacterium Pseudoalteromonas tetraodonis IAM 14160(T).

O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Pseudoalteromonas tetraodonis type strain IAM 14160(T) and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, 1H,(13)C HMQC and HMBC experiments. The polysaccharide was found to consist of hexasaccharide repeating units containing one residue each of D-Gal, D-GlcA, D-GalNAc and D-GlcNAc and two residues of 3,6-dideoxy-L-xylo-hexose (colitose, Col) and having the following structure:In common with the polysaccharides of some other bacteria, the polysaccharide studied contains a tetrasaccharide fragment alpha-Colp-(1-->2)-beta-D-Galp-(1-->3)-[alpha-Colp-(1-->4)]-beta-D-GlcpNAc, which is a colitose ('3-deoxy-L-fucose') analogue of the Lewis(b) blood group antigenic determinant.

[Structure of the repeating unit of the intracellular polysaccharide from Mycobacterium convolutum 240]. / Struktura povtoriaiushchegosia zvena vnekletochnogo polisakharida Mycobacterium convolutum 240.

An acidic extracellular polysaccharide composed of D-glucose, L-fucose, D-glucuronic acid and pyruvic acid residues has been isolated from the Mycobacterium convolutum culture fluid. On the basis of methylation analysis and 13C NMR data, the structure of the repeating unit of the polysaccharide was deduced as follows: -->3)-beta-D-Glcp-(1-->4)-beta-D-GlcpA-(1-->4)-alpha-L-Fucp- (1--> [formula: see text]

[Structure of the O-specific polysaccharide chain of the lipopolysaccharide from Yersinia intermedia serotype O:4.33]. / Struktura O-spetsificheskoi polisakharidnoi tsepi lipopolisakharida Yersinia intermedia serovara O: 4,33.

O-specific polysaccharide has been isolated on autohydrolysis of lipopolysaccharide from Yersinia intermedia O: 4.33 (strain 1476) and shown to consist of the yersiniose B (3.6-dideoxy-4-C-(1-hydroxyethyl)-xylo-hexose) and 2-acetamido-2-deoxy-D-galactose residues in a molar ratio of 1 : 2. Acid hydrolysis, methylation. solvolysis with anhydrous hydrogen fluoride. and 13C-NMR studies indicate the polysaccharide to be composed of trisaccharide repeating units of the following structure: (Formula: see text).

[Structure of O-specific polysaccharide chains of the Yersinia bercoviery O:10 lipopolysaccharide]. / Struktura O-spetsificheskikh polisakharidnykh tsepei lipopolisakharida Yersinia bercoviery 0:10.

O-specific polysaccharide composed of D-rhamnose and 3,6-dideoxy-4-C-(L- glycero-1-hydroxyethyl)-D-xylo-hexopyranose (yersiniose A) residues was obtained on mild acid degradation of the Yersinia bercovieri lipopolysaccharide. On the basis of 1H- and 13C-NMR data, methylation studies and Smith degradation, the following structure was suggested for the polysaccharide repeating unit: [formula: see text]

[Structure of the O-specific polysaccharide chain of Yersinia aldovae lipopolysaccharide]. / Struktura O-spetsificheskoi polisakharidnoi tsepi lipopolisakharida Yersinia aldovae.

O-specific polysaccharide has been isolated on mild hydrolysis of lipopolysaccharide from Yersinia aldovae and shown to consist of 2-acetamido-2-deoxy-D-glucose, D-glucose, 2-acetamido-2-deoxy-D-galactose, and 3,6-dideoxy-3- [(R)-3-hydroxybutyramido]-D-galactose in molar ratio 2:2:1:1. Acid hydrolysis, methylation, solvolysis with anhydrous hydrogen fluoride, 1H and 13C NMR studies indicated the polysaccharide to be composed of hexasaccharide repeating units of the following structure: [formula see text].

[A new branched-chain monosaccharide from the Yersinia enterocolitica serotype O:4.32 lipopolysaccharide]. / Novyi razvetvlennyi monosakharid iz lipopolisakharida Yersinia enterocolitica serovara O:4,32.

A component of the Yersinia enterocolitica O:4,32 lipopolysaccharide has been shown to be a second representative of the new class of monosaccharides and possess the structure of 3,6-dideoxy-4C-(1-hydroxyethyl)-D-xylo-hexose (yersiniose).

[The structure of the O-specific polysaccharide chain of the lipopolysaccharide of Yersinia pseudotuberculosis serovar VII]. / Stroenie O-spetsificheskoi polisakharidnoi tsepi lipopolisakharida Yersinia pseudotuberculosis serovara VII.

An O-specific polysaccharide of Yersinia pseudotuberculosis serovar VII has been isolated and characterized. The polysaccharide consists of colitose, D-glucose and 2-acetamido-2-deoxy-D-galactose in the ratio 1 : 2 : 2. From the results of methylation analysis, partial acid hydrolysis, 1H and 13C NMR spectroscopy the structure of the repeating unit of the O-specific polysaccharide is deduced as follows:

[Structure of the O-specific polysaccharide of the Yersinia enterocolitica serovar O:4.32 lipopolysaccharide. Serologic relations of lipopolysaccharides of Y. enterocolitica O:4.32 and Y. intermedia O:4.33]. / Struktura O-spetsificheskogo polisakharida lipopolisakharida Yersinia enterocolitica serovara O:4.32. Serologicheskoe rodstvo lipopolisakharidov Y. enterocolitica O:4.32 i Y. intermedia O:4.33.

O-Specific polysaccharide has been isolated on mild acid hydrolysis of the lipopolysaccharide from Yersinia enterocolitica O: 4.32 (strain 96) and shown to consist of yersiniose B (3,6-dideoxy-4-C-(1-hydroxyethyl)-D-xylo-hexose, YerB) acetylated at C1' and 2-acetamido-2-deoxy-D-galactose residues in a molar ratio 1:2. Acid hydrolysis, methylation and 13C NMR studies indicated the polysaccharide to be composed of trisaccharide repeating units of the following structure: (sequence; see text) The data obtained revealed structural and serological interrelation between O-antigens of Y. enterocolitica O:4.32 and Y. intermedia O:4.33.

[The structure of O-specific polysaccharide of Yersinia frederiksenii serotype O:16,29 lipopolysaccharide]. / Struktura O-spetsificheskogo polisakharida lipopolisakharida Yersinia frederiksenii serovara O:16,29.

A branched chain octose, 3,6-dideoxy-4-C-(L-glycero-1'-hydroxyethyl)-D-xylo- hexose, was isolated from the lipopolysaccharide of Yersinia frederiksenii, serovar O: 16.29 and identified as yersiniose A from Y. pseudotuberculosis, serovar VI. Mild hydrolysis of the lipopolysaccharide with acetic acid afforded a rhamnan. Structural features of the trisaccharide repeating unit were elucidated on the basic of 13C NMR spectral data, methylation studies and periodate oxidation. Using these data as well as data on sugar composition and methylation studies of the lipopolysaccharide, the following structural pattern of the repeating unit of O-specific polysaccharide was proposed: (formula; see text)

[Structure of the O-specific polysaccharide of Vibrio fluvialis serovar 3]. / Struktura O-spetsificheskogo polisakharida Vibrio fluvialis serovara 3.

O-Specific polysaccharide composed of L-rhamnose and 2-acetamido-2-deoxy-D-mannose was obtained on mild acid degradation of the V. fluvialis lipopolysaccharide. On the basis of the 13C-NMR data and methylation studies, the following structure was suggested for the polysaccharide repeating unit: ----4)-alpha-L-Rhap-(1----3)-beta-D-ManpNAc-(1---- This structure was confirmed by calculations using known glycosidation effects on 13C chemical shifts.

[Structure of the Yersinia rohdei O-specific polysaccharide]. / Struktura O-spetsificheskogo polisakharida Yersinia rohdei.

An O-specific polysaccharide composed of L-rhamnose was obtained on mild acid degradation of the Yersinia rohdei lipopolysaccharide. On the basis of methylation studies and 13C NMR data, the O-specific polysaccharide is shown to be a linear L-rhamnan built up of trisaccharide repeating units of the following structure: -->3)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap-(1-->3)-beta-L-Rh ap-(1--> The structure was confirmed by calculations with the use of the known glycosylation effect on 13C chemical shifts.

[Study of a lipopolysaccharide from Yersinia pseudotuberculosis of the IVA serotype]. / Izuchenie lipopolisakharida Yersinia pseudotuberculosis IVA-serovara.

The comparative studied on lipopolysaccharides from Yersinia pseudotuberculosis IVA serovar, strains 32 and 31D, have been conducted. The identity of the lipopolysaccharides isolated from these strains has been shown. The structural pattern of the repeating unit of the O-specific side chain of the lipopolysaccharide has been suggested: (Formula: see text)

[Structure of O-specific polysaccharide from Yersinia pseudotuberculosis of serotype VI lipopolysaccharide]. / Struktura O-spetsificheskogo polisakharida iz lipopolisakharida Yersinia pseudotuberculosis VI serovara.

Using methylation studies, partial hydrolysis and 13C NMR spectroscopy data, the following structure of O-specific polysaccharide from lipopolysaccharide of Yersinia pseudotuberculosis VI serovar has been proposed: (Formula: see text).

[Structure of the repeating link of the acid polysaccharide of Alteromonas haloplanktis KMM 156]. / Struktura povtoriaiushchegosia zvena kislogo polisakharida Alteromonas haloplaktis KMM 156.

An acidic capsular and an O-specific polysaccharide were isolated from the marine microorganism Alteromonas haloplanktis KMM 156. Both polysaccharides have the identical structure and are built up of tetrasaccharide repeating units, containing two residues of L-rhamnose as well as a 2-acetamido-2-deoxy-D-glucose and a 3-O-[(R)-1-carboxyethyl]-D-glucose (Glc3Lac) residue. On the basis of methylation studies, 1H- and 13C-NMR-spectroscopy including nuclear Overhauser effect and two-dimensional heteronuclear 13C/1H correlation spectroscopy, the following structure was suggested for the polysaccharide repeating unit: [formula: see text]