RESUMO
In this study, the complete nucleotide sequence of Chinese indigenous dog mitochondrial genome was determined for the first time. Sequence analysis showed that the genome structure was in accordance with other dogs. It contained 22 tRNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and 1 control region (D-loop region). The base composition was A (31.6%), G (14.2%), C (25.5%) and T (28.7%), so the percentage of A and T (60.3%) was higher than that of G and C. The complete mitochondrial genome sequence of the Chinese indigenous dog would shed a new light on further studies in dog domestication.
Assuntos
Genoma Mitocondrial/genética , Análise de Sequência de DNA , Animais , Animais Domésticos , Cães , Genes de RNAr , Anotação de Sequência Molecular , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , RNA de Transferência/genéticaRESUMO
In this study, the whole mtDNA genome of Simao Chinese indigenous dog was amplified and sequenced. Our data showed that the whole mtDNA genome of Simao Chinese indigenous dog includes 16,730 base pairs (bps). The Simao Chinese indigenous dog mitochondrial genome included structural organization and base composition of the rRNAs, tRNAs and protein-coding genes, as well as characteristics of tRNAs.