Perturbations to common gardens of anaerobic co-digesters reveal relationships between functional resilience and microbial community composition.

We report the relationship between enrichment of adapted populations and enhancement of community functional resilience in methanogenic bioreactors. Although previous studies have shown the positive effects of acclimation, this work directly investigated the relationships between microbiome dynamics and performance of anaerobic co-digesting reactors in response to different levels of an environmental perturbation (loading of grease interceptor waste [GIW]). Using the methanogenic microbiome from a full-scale digester, we developed eight sets of microbial communities in triplicate using different feed sources. These substrate-specific microbiomes were then exposed to three independent disturbance events of low-, mid- and high-GIW loading rates. This approach allowed us to directly attribute differences in community responses to differences in community composition. Despite identical inocula, environment (digester operation, substrate loading rate, and feeding patterns) and general whole-community function (methane production and effluent quality) during the cultivation period, different substrates led to different microbial community assemblies. Lipid pre-acclimation led to enrichment of a pool of specialized populations, along with thriving of sub-dominant communities. The enrichment of these populations improved functional resilience and process performance when exposed to a low level of lipid-rich perturbation compared with less-acclimated communities. At higher levels of perturbation, the communities were not able to recover methanogenesis, indicating a loading limit to the resilience response. This study extends our current understanding of environmental perturbations, feed-specific adaptation, and functional resilience in methanogenic bioreactors.IMPORTANCEThis study demonstrates, for the first time for GIW co-digestion, how applying similar perturbations to different microbial communities was used to directly identify the causal relationships between microbial community, function, and environment in triplicate anaerobic microbiomes. We evaluated the impact of feed-specific adaptation on methanogenic microbiomes and demonstrated how microbiomes can be influenced to improve their functional (methanogenic) resilience to GIW inhibition. These findings demonstrate how an ecological framework can help improve a biological engineering application, and more specifically, increase the potential of anaerobic co-digestion for converting wastes to energy.

Utilization strategy for algal bloom waste through co-digestion with kitchen waste: Comprehensive kinetic and metagenomic analysis.

Anaerobic co-digestion (AcoD) with kitchen waste (KW) is an alternative utilization strategy for algal bloom waste (AW). However, the kinetic characteristic and metabolic pathway during this process need to be explored further. This study conducted a comprehensive kinetic and metagenomic analysis for AcoD of AW and KW. A maximum co-digestion performance index (CPI) of 1.13 was achieved under the 12% AW addition. Co-digestion improved the total volatile fatty acids generation and the organic matter transformation efficiency. Kinetic analysis showed that the Superimposed model fit optimally (R2Adj = 0.9988-0.9995). The improvement of the kinetic process by co-digestion was mainly reflected in the increase of the methane production from slowly biodegradable components. Co-digestion enriched the cellulolytic bacterium Clostridium and the hydrogenotrophic methanogenic archaea Methanobacterium. Furthermore, for metagenome analysis, the abundance of key genes concerned in cellulose and lipid hydrolysis, pyruvate and methane metabolism were both increased in co-digestion process. This study provided a feasible process for the utilization of AW produced seasonally and a deeper understanding of the AcoD synergistic mechanism from kinetic and metagenomic perspectives.

Agronomic and phytotoxicity test with biosolids from anaerobic CO-DIGESTION with temperature and micro-organism phase separation, based on sewage sludge, vinasse and poultry manure.

This study deals with energy and agronomic valorisation by anaerobic co-digestion with temperature and microorganism phase separation of sewage sludge, vinasse and poultry manure, with the aim of achieving an integral waste management, obtaining bioenergy and biofertilizer that returns nutrients to the soil in a natural way. The yields obtained were 40 mL H2/gVS and 391 mLCH4/gVS. The resulting effluent showed more than 98 % removal of E. coli and Total Coliforms, as well as total removal of Salmonella. The results obtained in the phytotoxicity tests showed that all the proportions studied had phytostimulant and phytonutrient properties, with 20 % having the highest germination index (GI) with mean values of 145.30 %. Finally, the agronomic trial carried out with strawberry crops (Fragaria sp.) showed that the addition of this biosolid has fertilising properties and can be used as an agronomic amendment, with an increase of 145 % in fresh weight and 102.5 % in dry weight, and fruit production doubled with respect to the control. The ANOVA statistical study corroborated that there were significant differences in crop growth when applying different proportions of biofertilizer in the fertilizer. Therefore, these results show that this technology is promising and would contribute environmentally, socially and economically to the transfer towards a circular economy model.

Enhanced methane production from source-separated human feces (brown water) by single phase anaerobic co-digestion: Effects of different co-substrates.

Based on the concept of source separation of brown water (BW, human feces with flushing water) and yellow water (urine) in rural area, anaerobic co-digestion of BW with agricultural waste is a promising and effective method for rural waste treatment and resource recovery. The purpose of this study was to investigate the performance of different agricultural wastes (peanut straw (PST), peanut shell (PSH), swine wastewater acting as co-substrate for anaerobic co-digestion with BW, and the relative mechanisms were explored. When the mixed ratio was uniformly set as 1:1 (mass ratio, measured by volatile solid (VS)) and initial VS load as 20 g/L, the maximum cumulative methane production obtained by co-digestion (21 days) of BW and PST was 688 mL/g-VS, which performed better than the individual substrates (341 mL/g-VS), as well as the average of the sole BW and sole PST groups (531.2 mL/g-VS). The most impactful advantage was ascribed to the promotion of hydrolytic and acidogenic enzyme activities. The addition of PST also reduced the production of endogenous humus, which is difficult for biodegradation. Microbial community analysis showed that different co-substrates would affect the microbial community composition in the reactor. The relative abundance of hydrolytic acidogens in the PST and PSH co-digestion groups were higher than that in the SW co-digestion and sole BW groups, and the methanogenic archaea were dominated by the acetate-trophic Methanotrichaceae. The overall results suggest that anaerobic co-digestion is a feasible method, and co-digestion of BW and PST can improve methane production potential.

Stabilization of microbial network by co-digestion of swine manure and organic wastes.

The production of biogas from organic waste has attracted considerable interest as a solution to current energy and waste management challenges. This study explored the methane (CH4) production potential of swine manure (SM), food waste (FW), and tomato waste (TW) and the changes in the microbial community involved in the anaerobic digestion process. The results revealed that the CH4 production potentials of the four kinds of SM samples were influenced by the characteristics of SM (e.g., age and storage period). Among the four kinds of SM samples, the CH4 yield from the manure directly sampled from primiparous sows (SM3) was the highest. The CH4 yield was significantly improved when SM3 was co-digested with FW, but not with TW. The addition of SM fostered a stable CH4 production community by enhancing the interaction between methanogens and syntrophic bacteria. Furthermore, the addition of FW as a co-substrate may improve the functional redundancy structure of the methanogenesis-associated network. Overall, the characteristics of SM must be considered to achieve consistent CH4 yield efficiency from anaerobic digestion since CH4 production potentials of SM can be different. Also, the contribution of co-substrate to the synergistic relationship between methanogens and syntrophic bacteria can be considered when a co-substrate is selected in order to enhace CH4 yield from SM.

The dominant-substrate driven the enhanced performance in co-digestion of Pennisetum hybrid and livestock waste.

Co-digestion has been considered a promising method to improve methane yield. The effect of the proportion of dominant substrate on the performance and microbial community of anaerobic digestion of Pennisetum hybrid (PH) and livestock waste (LW) was investigated. An obvious synergistic effect was obtained with an increase of 15.20%-17.45% in specific methane yield compared to the predicted value. Meanwhile, the dominant substrate influenced the relational model between methane yield enhancement rate and mixture ratio. For the LW-dominant systems, a parabolic model between enhancement rate and mixture ratio was observed with a highest value of 392.16 mL/g VS achieved at a PH:LW ratio of 2:8. While a linear pattern appeared for PH-dominant systems with the highest methane yield of 307.59 mL/g VS. Co-digestion selectively enriched the relative abundance of Clostridium_sensu_stricto_1, Terrisporobacter, Syntrophomonas, Methanosarcina and Methanobacterium, which boosted the performance of hydrolysis, acidogenesis, acetogenesis and methanogenesis processes.

Enhanced anaerobic co-digestion of food waste and sewage sludge by co-application of biochar and nano-Fe3O4.

Conductive materials have been utilized to facilitate direct interspecies electron transfer (DIET) in anaerobic digestion (AD) to enhance methane production. However, the impact and efficacy of the co-application of biochar and nano-Fe3O4 have not been adequately elucidated, particularly their interaction on electron transfer efficiency. In this investigation, we examined the influence of simultaneously or independently adding biochar and nano-Fe3O4 to food waste (FW) and sewage sludge (SS) anaerobic co-digestion. A synergistic effect was observed under the co-application condition. Methane production reached 300.3 ± 19.8 mL/gCOD with the co-application of biochar and nano-Fe3O4, representing a 43.3%, 35.4%, and 5.4% increase compared to the sole Fe3O4, biochar, and nano-Fe3O4, respectively. Mechanistic analysis revealed that, in comparison to sole biochar and nano-Fe3O4, their co-occurrence significantly accelerated hydrolysis and acidogenesis, thereby enhancing the release of soluble organic components. Furthermore, the application of nano-Fe3O4 improved system stability and significantly promoted propionate degradation, maintaining a favorable condition for methane production. Additionally, the noteworthy increase in INT-ETS activity and cytochrome c concentration indicated that the co-application of biochar and nano-Fe3O4 stimulated electron transfer. Correspondingly, the activity of coenzyme F420, which indicates the performance of methanogenesis, exhibited a 2.44-fold increase compared to the control. This indicated that nano-Fe3O4 and biochar co-amendment can serve as a robust platform to strengthen DIET. This study provided a new insight regarding the application of biochar and nano-Fe3O4 in the AD system for strengthening electron transfer to promote methane production.

Enhancing biogas production from hemp biomass residue through hydrothermal pretreatment and co-digestion with cow manure: Insights into methane yield, microbial communities, and metabolic pathways.

This study investigates the enhancement of biogas production from hemp biomass residue (HBR) through hydrothermal pretreatment and co-digestion with cow manure (CM). Hydrothermal pretreatment at 200 °C for 15 min significantly improved the methane yield from 311.5 to 434.3 mL-CH4/g-VSadded (p ≤ 0.05) from HBR at 10% total solids (TS) loading, a 39% increase. Co-digestion with CM at an optimum ratio of 80:20 further increased the methane yield (738.7 mL-CH4/g-VSadded), representing a 70% improvement over pretreated HBR alone and a 137% increase compared to untreated HBR. Microbial community analysis revealed the dominance of Methanosaeta, comprising 83-93% of archaeal genera across samples. Gene expression analysis showed acetoclastic methanogenesis as the dominant pathway, accounting for 80% of methanogenesis sequences. Hydrogenotrophic methanogenesis and CO2 reduction with H2 pathways contributed 10% each. The optimized process achieved a biodegradation efficiency of 94% for hydrothermally pretreated HBR, compared to 68% for untreated HBR. Mass balance analysis demonstrated that combining hydrothermal pretreatment with anaerobic digestion increased biogas yield from 79% for untreated HBR to 86% for pre-treated HBR (PHBR) co-digested with CM. Integrating hydrothermal pretreatment and co-digestion enhances biogas production from lignocellulosic agricultural residues, contributing to sustainable waste management and renewable energy production.

Microbial community changes and metabolic pathways analysis during waste activated sludge and meat processing waste anaerobic co-digestion.

Waste activated sludge (WAS) and meat processing waste (MPW) were acted as co-substrates in anaerobic co-digestion (AcD), and biochemical methane potential (BMP) test was carried out to investigate the methane production performances. Microbial community structure and metabolic pathways analyses were conducted by 16S rRNA high-throughput sequencing and functional prediction analysis. BMP test results indicated that AcD of 70% WAS+30% MPW and 50% WAS+50% MPW (VS/VS) could significantly improve methane yield to 371.05 mL/g VS and 599.61 mL/g VS, respectively, compared with WAS acting as sole substrate (191.87 mL/g VS). The results of microbial community analysis showed that Syntrophomonas and Petrimonas became the dominant bacteria genera, and Methanomassiliicoccus and Methanobacterium became the dominant archaea genera after MPW addition. 16S functional prediction analysis results indicated that genes expression of key enzymes involved in syntrophic acetate oxidation (SAO), hydrogenotrophic and methylotrophic methanogenesis were up-regulated, and acetoclastic methanogenesis was inhibited after MPW addition. Based on these analyses, it could be inferred that SAO combined with hydrogenotrophic and methylotrophic methanogenesis was the dominant pathway for organics degradation and methane production during AcD. These findings provided systematic insights into the microbial community changes and metabolic pathways during AcD of WAS and MPW.

Synergistic effect and microbial community structure of waste-activated sludge and kitchen waste solids residue mesophilic anaerobic co-digestion.

Anaerobic co-digestion was conducted on the solid residues after three-phase separation of kitchen waste (KWS) and waste-activated sludge (WAS), the synergistic effects and process performance were studied during co-digestion at different ratios of KWS to WAS. KWS and WAS mix ratios of 0:1, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1 and 1:0 (based on TS). The results showed that a ratio of KWS to WAS of 1:1 got a very high methane recovery with a methane yield of 310.45 ± 30.05 mL/g VSadded. The highest concentration of free ammonia among all reaction systems was only 70.23 ± 5.53 mg/L, which was not enough to produce ammonia inhibition in the anaerobic co-digestion system. However, when the KWS content exceeded 50%, methane inhibition and prolongation of the lag phase were observed due to the accumulation of volatile fatty acids (VFAs), and during the lag phase. Microbial community analysis showed that various bacterial groups involved in acid production and hydrolysis were mainly dominated by phylum Firmicutes, Chloroflexi, Proteobacteria and Bacteroidetes. Hydrogenotrophic methanogen was found to dominate all archaeal communities in the digesters. Co-digestion of KWS with WAS significantly increased the relative abundance of Methanobacterium compared with anaerobic digestion of WAS alone.

Source separation and anaerobic co-digestion of blackwater and food waste for biogas production and nutrient recovery.

Anaerobic co-digestion of source-separated blackwater (BW) and food and kitchen waste (FW) offers decentralized circular economy solutions by enabling local production of biogas and nutrient-rich byproducts. In this study, a 2 m3 pilot-scale continuously stirred tank reactor (CSTR) operated under mesophilic conditions was utilized for co-digestion of BW and FW. The process obtained a CH4 yield of 0.7 ± 0.2 m3/kg influent-volatile solid (VS), reaching a maximum yield of 1.1 ± 0.1 m3/kg influent-VS, with an average organic loading rate of 0.6 ± 0.1 kg-VS/m3/d and HRT of 25 days. The CH4 production rate averaged 0.4 ± 0.1 m3/m3/d, peaking at 0.6 ± 0.1 m3/m3/d. Treatment of digestate through flocculation followed by sedimentation recovered over 90% of ammonium nitrogen and potassium, and 80-85% of total phosphorus in the liquid fraction. This nutrient-rich liquid was used to cultivate Chlorella vulgaris, achieving a biomass concentration of 1.2 ± 0.1 g/L and 85 ± 3% and 78 ± 5% ammonium nitrogen and phosphorus removal efficiency, respectively. These findings not only highlight the feasibility of anaerobic co-digestion of source-separated BW and FW in local biogas production but also demonstrate the potential of microalgae cultivation as a sustainable approach to converting digestate into nutrient-rich algae biomass.

Coupling of biogas residue biochar and low-magnitude electric fields promotes anaerobic co-digestion of sewage sludge and food waste.

Biochar-assisted anaerobic digestion (AD) remains constrained due to the inefficient decomposition of complex organics, even with the direct interspecies electron transfer (DIET) pathway. The coupling of electrochemistry with the anaerobic biological treatment could shorten lengthy retention time in co-digestion by improving electron transfer rates and inducing functional microbial acclimation. Thus, this work investigated the potential of improving the performance of AD by coupling low-magnitude electric fields with biochar derived from the anaerobically digested biogas residue. Different voltages (0.3, 0.6, and 0.9 V) were applied at various stages to assess the impact on biochar-assisted AD. The results indicate that an external voltage of 0.3 V, coupled with 5 g/L of biochar, elevates CH4 yield by 45.5% compared to biogas residue biochar alone, and the coupled approach increased biogas production by up to 143% within 10 days. This finding may be partly explained by the enhanced utilization of substrates and the increased amounts of specific methanogens such as Methanobacterium and Methanosarcina. The abundance of the former increased from 4.0 to 11.3%, which enhances the DIET between microorganisms. Furthermore, the coupling method shows better potential for enhancing AD compared to preparing iron-based biochar, and these results present potential avenues for its broader applications.

Hydrothermal and thermal-alkali pretreatments of wheat straw: Co-digestion, substrate solubilization, biogas yield and kinetic study.

Agro-waste having lignocellulosic biomass is considered most effective (heating value 16 MJ/kg) for energy production through anaerobic digestion (AD). However, recalcitrant lignocellulosic fraction in agro-waste obstructs its biotransformation and is a rate-limiting step of the process. This study investigated the effects of hydrothermal and thermal-alkaline pretreatment on anaerobic co-digestion of wheat straw (WS). The hydrothermal pretreatment of WS revealed that 60 min was the best pretreatment time to achieve the highest substrate solubilization. It was employed for thermal-alkali pretreatment at variable temperatures and NaOH doses. Thermal-alkali pretreatment at 125°C-7% NaOH shows the highest (34%) biogas yield of 662 mL/gVS, followed by 646 mL/gVS biogas yield at 150°C-1% NaOH assay (31% higher) over control. Although the 125°C-7% NaOH assay achieved the highest biogas yield, the 150°C-1% NaOH assay was found more feasible considering the cost of a 6% higher chemical used in the earlier assay. The thermal-alkali pretreatment was observed to reduce the formation of recalcitrant compounds (HMF, Furfural) and increase the buffering capacity of the slurry over hydrothermal pretreatment. Principal component analysis (PCA) of the various pretreatment and AD operational parameters was carried out to study their in-depth correlation. Moreover, a kinetic study of the experimental data was performed to observe the biodegradation trend and compare it with the Modified Gompertz (MG) and First Order (FO) models.

Sugarcane-bagasse-ash in enhanced mesophilic Co-digestion for biogas and nutrient recovery: A concept of developing rural circular bioeconomy.

Conductive agro-industrial wastes as accelerants in the anaerobic digestion (AD) of organic waste is a good technique for developing a rural circular economy, such as producing bioenergy and biofertilizer. This study disclosed the a role of sugar cane bagasse ash (SCBA) in enhancing the bioenergy (biogas) yield and digestate fertility via anaerobic co-digestion (AcoD) of buffalo dung (BD) and vegetable residue (VR) under mesophilic conditions (37 á´¼C). Firstly, an optimal BD/VR ratio (1:3) was determined based on biogas yield by introducing five different BD/VR ratios (1:0, 3:1, 1:1, 1:3, and 0:1) into AcoD systems. Secondly, the biogas yield was increased further by adding SCBA at five different concentrations (0, 0.5, 1, 1.5, and 2 wt%). Experimental results disclosed that the 1.5 wt% of SCBA gave the highest cumulative biogas yield (153.67 mL/g VS), COD removal rate (31.18%), and fertility (5.08%). Moreover, a framework is suggested to understand the role of SCBA in the enhanced DIET mechanism. This work documents an environmentally friendly and economical technique for developing a rural circular bioeconomy via the AD of organic agro-waste.

Exploring the potential of thermophilic anaerobic co-digestion between agro-industrial waste and water hyacinth: operational performance, kinetic study and degradation pathway.

Anaerobic co-digestion (co-AD) of agro-industrial waste, namely, palm oil mill effluent (POME) and sugarcane vinasse (Vn), with water hyacinth (WH) as co-substrate was carried out in two separate Anaerobic Suspended Growth Closed Bioreactors (ASGCBs) under thermophilic (55 °C) conditions. The highest chemical oxygen demand (COD) and soluble COD reduction in co-AD of POME-WH (78.61%, 78.86%) is slightly higher than co-AD of Vn-WH (75.75%, 78.24%). However, VFA reduction in co-AD of POME-WH (96.41%) is higher compared to co-AD of Vn-WH (85.94%). Subsequently, biogas production peaked at 13438 mL/day values and 16122 mL/day for co-AD of POME-WH and Vn-WH, respectively. However, the methane content was higher in the co-AD of POME-WH (72.04%) than in the co-AD of Vn-WH (69.86%). Growth yield (YG), maximum specific substrate utilization rate (rx,max) and maximum specific biomass growth rate (µmax) are higher in co-AD of POME-WH, as supported by the higher mixed liquor volatile suspended solids (MLVSS) and COD reduction efficiency compared to co-AD of Vn-WH. However, methane yield ([Formula: see text]) reported in the co-AD of POME-WH and Vn-WH are 0.2748 and 0.3112 L CH4/g CODreduction, respectively, which suggests that WH is a more suitable co-substrate for Vn compared to POME.

Anaerobic digestion of vinasse and water treatment plant sludge increases methane production and stability of UASB reactors.

Studies are still needed to increase the stability and efficiency of methane production from vinasse. Therefore, operations strategies, such as the anaerobic digestion with one or more wastes and adding micronutrients, especially iron, become attractive. The performance of two treatment systems, each one composed of two UASB reactors in series, operated under mesophilic (R1M and R2M) and thermophilic (R1T and R2T) temperature conditions, was evaluated in the anaerobic digestion of vinasse (ADV). First, the reactors were operated with the effluent recirculation and increasing organic loading rate (OLR) up to 20 g CODtotal L-1d-1 in the R1M and R1T. Then, the anaerobic digestion of vinasse and water treatment plant (WTP) sludge (ADVS) was performed in the proportions of 25:75 to 50:50 (% v/v) in both systems. In the ADV, applying the highest OLR, the mesophilic and thermophilic reactors instabilities happened. The ADVS of over 35% of WTP sludge promoted the recovery of the mesophilic and thermophilic UASB reactors with significantly reduced total volatile acids and increased alkalinity and biogas production. The higher average values of the volumetric methane production (VMP) occurred in the ADVS at 50% of WTP; in the R1M and R1T, they were 3.23 and 3.00 L CH4 L-1d-1, respectively. In the ADV, the thermophilic system presented higher VMP concerning the mesophilic for OLR up to 15 g CODtotal L-1d-1. For higher OLR, the mesophilic system showed better carrying capacity and stability. The ADVS with above 35% of WTP sludge promoted similar benefits in the two systems, with no significant differences in CODtotal removal and VMP. Therefore, adding iron by WTP sludge in ADVS improves methane production and increases the stability of UASB reactors under mesophilic and thermophilic conditions.

A Review on Performance Improvement of Anaerobic Digestion Using Co-Digestion of Food Waste and Sewage Sludge.

Anaerobic co-digestion (AcoD) is a vital technology in the decarburization of the economy because of its ability to process organic waste, recover nutrients, and create biogas as a sustainable biofuel all at the same time. This attribute also makes this technology a viable partner in pursuing a circular economic model. However, the poor biogas output of typical substrates like sewage sludge and animal manure and the hefty installation costs limit its viability. This review paper with literature analysis provides a good grasp of the anaerobic co-digesting process with diverse food digestion methods. In this survey, we have analyzed the Anaerobic Digestion of water waste, food waste, and animal manure and the anaerobic co-digestion of animal waste with water waste and food waste with water waste. This analysis demonstrates that anaerobic co-digestion produces more methane biogas than anaerobic digestion. Also, it has been shown that by adjusting the ratio of food and animal waste to water waste, we can produce more methane. In the future, we would like to supplement anaerobic co-digestion by altering the proportion of different wastes that are mixed with water waste in order to increase methane production.

Recent advances in co-digestion conjugates for anaerobic digestion of food waste.

Anaerobic digestion (AD) is a biological process that breaks down organic waste materials, such as food waste (FW) that produces biogas and digestate. The biogas can be utilized as biofuel, and digestate could be applied as fertilizer. However, AD of FW alone has limitations on optimal degradation, digester stability and biogas yield. Co-digestion of FW along with other organic wastes such as animal manure, agricultural residue, sewage sludge and industrial organic waste, has shown substantial improvement in degradation process with increased biogas yield. The inadequacies in FW for optimum AD, like low carbon-to-nitrogen ratio (C/N ratio), lack of trace elements and irregular particle sizes, can be nullified by adding appropriate co-digestion conjugates. This review aims to describe the characteristic inadequacies of FW and examines the effect on mesophilic co-digestion of FW with animal manure, waste sludge and agricultural wastes for biogas production optimization. A critical review on the impact of pretreatment and co-digestion to enrich the methane (CH4) content in biogas has been performed. The review also examines the microbial community shift due to co-digestion, which is critical for the stability of an anaerobic digester. Finally, it discusses the prospects and challenges for the widespread application of the co-digestion technique as an effective organic waste management practice.

Anaerobic Co-digestion of sewage sludge and organic fraction of municipal solid waste: Focus on mix ratio optimization and synergistic effects.

The utilization of municipal solid waste (MSW) and sewage sludge (SS) as a source of renewable energy is crucial in achieving sustainable and integrated MSW management. SS and organic fraction of municipal solid waste (OFMSW) can be anaerobically digested to produce methane for energy. However, anaerobic digestion of specific substrates is challenging with respect to substrate characteristics. The problem of mono-digestion can be mitigated by co-digestion of these two major organic wastes because of their complementary characteristics. Moreover, there is a lack of studies on optimization of different mix ratios of organic fraction of municipal solid waste (OFMSW) and sewage sludge (SS) based on total solids (TS). The study aims to optimize the mix ratio for anaerobic co-digestion of OFMSW and SS. The study further elucidates synergistic effects associated with the co-digestion process. Different mix ratios of SS and OFMSW (0:100, 20:80, 40:60, 60:40, 80:20, 100:0) at 5, 7.5 and 10% TS were assessed for biomethane potential assessment. The results showed that with an increase in SS in the mix ratio feed the methane yield increased by 91% and 50% as compared to mono digestion of sewage sludge and OFMSW respectively at TS 7.5%. Based on the kinetic analysis, it was observed that the lag phase reduced for 60:40 mix ratio leading to higher rate of biodegradation. Positive synergistic effects were observed for 40:60, 60:40 and 80:20 mix ratio of SS:OFMSW based on co-digestion impact factor value. Response surface modelling was used to get the optimized mix ratio and TS %. The optimum mix ratio with the highest methane yield (388 ml/gVS added) was 70:30 (SS: OFMSW) at TS 7.5% with a desirability value of 0.98. These findings demonstrate that co-digesting SS and OFMSW is a preferable alternative for harnessing renewable energy and managing organic waste in a sustainable manner.

Enhanced methane production from the co-digestion of food waste and thermally hydrolyzed sludge filtrate.

Although many technologies can be applied to sewage sludge (SS) and food waste (FW) treatment, high investment and operational costs, high land occupation, and the "not-in-my-backyard" effect pose many challenges in practice. Thus, it is important to develop and utilize low-carbon or negative-carbon technologies to tackle the carbon problem. This paper proposes a method of anaerobic co-digestion of FW and SS, thermally hydrolyzed sludge (THS), or THS filtrate (THF) to enhance their methane potential. Compared to the co-digestion of SS with FW, the methane yield of the co-digestion of THS and FW was 9.7-69.7% higher, and that of the co-digestion of THF and FW was 11.1-101.1% higher. The synergistic effect was weakened with the addition of THS but enhanced with the addition of THF, potentially owing to the change in humic substances. Filtration removed most humic acids (HAs) from THS but retained fulvic acids (FAs) in THF. Moreover, THF produced 71.4% of the methane yield of THS, although only 25% of the organic matter permeated from THS to THF. This indicated that hardly biodegradable substances remained in the dewatering cake and were removed from anaerobic digestion systems. The results indicate that the co-digestion of THF and FW is an effective way to enhance methane production.