Microplastics increase cadmium absorption and impair nutrient uptake and growth in red amaranth (Amaranthus tricolor L.) in the presence of cadmium and biochar.

Microplastic (MP) pollution in terrestrial ecosystems is gaining attention, but there is limited research on its effects on leafy vegetables when combined with heavy metals. This study examines the impact of three MP types-polyethylene (PE), polyethylene terephthalate (PET), and polystyrene (PS)-at concentrations of 0.02, 0.05, and 0.1% w/w, along with cadmium (Cd) and biochar (B), on germination, growth, nutrient absorption, and heavy metal uptake in red amaranth (Amaranthus tricolor L.). We found that different MP types and concentrations did not negatively affect germination parameters like germination rate, relative germination rate, germination vigor, relative germination vigor, and germination speed. However, they increased phytotoxicity and decreased stress tolerance compared to an untreated control (CK1). The presence of MPs, particularly the PS type, reduced phosphorus and potassium uptake while enhancing Cd uptake. For example, treatments PS0.02CdB, PS0.05CdB, and PS0.1CdB increased Cd content in A. tricolor seedlings by 158%, 126%, and 44%, respectively, compared to the treatment CdB (CK2). Additionally, MP contamination led to reduced plant height, leaf dry matter content, and fresh and dry weights, indicating adverse effects on plant growth. Moreover, the presence of MPs increased bioconcentration factors and translocation factors for Cd, suggesting that MPs might act as carriers for heavy metal absorption in plants. On the positive side, the addition of biochar improved several root parameters, including root length, volume, surface area, and the number of root tips in the presence of MPs, indicating potential benefits for plant growth. Our study shows that the combination of MPs and Cd reduces plant growth and increases the risk of heavy metal contamination in food crops. Further research is needed to understand how different MP types and concentrations affect various plant species, which will aid in developing targeted mitigation strategies and in exploring the mechanisms through which MPs impact plant growth and heavy metal uptake. Finally, investigating the potential of biochar application in conjunction with other amendments in mitigating these effects could be key to addressing MP and heavy metal contamination in agricultural systems.

Advancing the Effect-Directed Identification in Combined Pollution: Using Pathways to Link Effects and Toxicants.

The difficulty in associating diverse pollutants with mixture effects has led to significant challenges in identifying toxicants in combined pollution. In this study, pathways were used to link effects and toxicants. By pathways evaluated by the concentration-dependent transcriptome, individual effects were extended to molecular mechanisms encompassing 135 pathways corresponding to 6 biological processes. Accordingly, mechanism-based identification of toxicants was achieved by constructing a pathway toxicant database containing 2413 chemical-pathway interactions and identifying pathway active fragments of 72 pathways. The developed method was applied to two different wastewaters, industrial wastewater OB and municipal wastewater HL. Although lethality and teratogenesis were both observed at the individual level, different molecular mechanisms were revealed by pathways, with cardiotoxicity- and genotoxicity-related pathways significantly enriched in OB, and neurotoxicity- and environmental information processing-related pathways significantly enriched in HL. Further suspect and nontargeted screening generated 59 and 86 causative toxicants in OB and HL, respectively, among which 29 toxicants were confirmed, that interacted with over 90% of enriched pathways and contributed over 50% of individual effects. After upgrading treatments based on causative toxicants, consistent removal of toxicants, pathway effects, and individual effects were observed. Mediation by pathways enables mechanism-based identification, supporting the assessment and management of combined pollution.

Effects of Zinc Thiazole and Oxytetracycline on the Microbial Metabolism, Antibiotic Resistance, and Virulence Factor Genes of Soil, Earthworm Gut, and Phyllosphere.

Pesticides and antibiotics are believed to increase the incidence of antibiotic resistance genes (ARGs) and virulence factor genes (VFGs), constituting a serious threat to global health. However, the impact of this combined pollution on the microbiome and that of the related ARGs and VFGs on soil-plant-animal systems remain unknown. In this study, a 60-day microcosm experiment was conducted to reveal the effects of zinc thiazole (ZT) and oxytetracycline (OTC) on microbial communities, antibiotic resistomes, and virulence factors in soil, earthworm gut, and phyllosphere samples using metagenomics. ZT exposure perturbed microbial communities and nutrient metabolism and increased the abundance of ARGs and VFGs in the gut. Combined exposure changed the profiles of ARGs and VFGs by decreasing microbial diversity in the phyllosphere. Host-tracking analysis identified some genera, such as Citrobacter and Aeromonas, as frequent hosts of ARGs and VFGs in the gut. Notably, some co-occurrence patterns of ARGs and MGEs were observed on the metagenome-assembled contigs. More importantly, ZT markedly increased the abundance of potentially drug-resistant pathogens Acinetobacter soli and Acinetobacter junii in the phyllosphere. Overall, this study expands our current understanding of the spread of ARGs and VFGs in soil-plant-animal systems under pollutant-induced stress and the associated health risks.

Co-toxicity and co-contamination remediation of polycyclic aromatic hydrocarbons and heavy metals: Research progress and future perspectives.

The combined pollution of polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) has attracted wide attention due to their high toxicity, mutagenicity, carcinogenicity and teratogenicity. A thorough understanding of the progress of the relevant studies about their co-toxicity and co-contamination remediation is of great importance to prevent environmental risk and develop new efficient remediation methods. This paper summarized the factors resulting in different co-toxic effects, the interaction mechanism influencing co-toxicity and the development of remediation technologies for the co-contamination. Also, the inadequacies of the previous studies related to the co-toxic effect and the remediation methods were pointed out, while the corresponding solutions were proposed. The specific type and concentration of PAHs and HMs, the specific type of their action object and environmental factors could affect their co-toxicity by influencing each other's transmembrane process, detoxification process and increasing reactive oxygen species (ROS) and some other mechanisms that need to be further studied. The specific action mechanisms of the concentration, environmental factors and the specific type of PAHs and HMs, their effect on each other's transmembrane processes, investigations at the cellular and molecular levels, non-targeted metabolomics analysis, as well as long-term ecological effects were proposed to be further explored in order to obtain more information about the co-toxicity. The combination of two or more methods, especially combining bioremediation with other methods, is a potential development field for the remediation of co-contamination. It can make full use of the advantages of each remediation method, to achieve an increase of remediation efficiency and a decrease of both remediation cost and ecological risk. This review intends to further improve the understanding on co-toxicity and provide references for the development and innovation of remediation technologies for the co-contamination of PAHs and HMs.

Removal of sulfamethoxazole and Cu, Cd compound pollution by arbuscular mycorrhizal fungi enhanced vertical flow constructed wetlands.

The combined pollution of antibiotics and heavy metals (HMs) has a serious impact on the water ecological environment. Previous researches mainly focused on the removal of antibiotics or HMs as single pollutants, with limited investigation into the treatment efficiencies and underlying mechanisms associated with their co-occurring pollution. In this study, 16 micro vertical flow constructed wetlands (MVFCWs) were constructed to treat composite wastewater consisting of sulfamethoxazole (SMX), copper (Cu) and cadmium (Cd), involving two different inoculation treatments (arbuscular mycorrhizal fungi (AMF) inoculated and uninoculated) and eight kinds of pollutant exposure (Control Check (CK), SMX, Cu, Cd, SMX + Cu, SMX + Cd, Cu + Cd, SMX + Cu + Cd). The findings of this study demonstrated that the inoculation of AMF in MVFCWs resulted in removal efficiencies of SMX, Cu, and Cd ranging from 18.70% to 80.52%, 75.18% to 96.61%, and 40.50% to 89.23%, respectively. Cu and CuCd promoted the degradation of SMX in the early stage and inhibited the degradation of SMX in the later stage. Cd did not demonstrate a comparable promotive impact on SMX degradation, and its addition hindered Cu removal. However, comparatively, the presence of Cu exerted a more pronounced inhibitory effect on Cd removal. Furthermore, the addition of Cu augmented the abundances of Proteobacteria, Bacteroidetes (at the phylum level) and Rhodobacter, Lacunisphaera and Flavobacterium (at the genus level), and Cu exposure showed a substantially stronger influence on the microbial community than that of Cd and SMX. AMF might confer protection to plants against HMs and antibiotics by enriching Nakamurella and Lacunisphaera. These findings proved that AMF-C. indica MVFCW was a promising system, and the inoculation of AMF effectively enhanced the simultaneous removal of compound pollution.

Molecular strategies of the pygmy grasshopper Eucriotettix oculatus adapting to long-term heavy metal pollution.

To study the heavy metal accumulation and its impact on insect exterior and chromosome morphology, and reveal the molecular mechanism of insects adapting to long-term heavy metal compound pollution habitats, this study, in the Diaojiang river basin, which has been polluted by heavy metals(HMs) for nearly a thousand years, two Eucriotettix oculatus populations was collected from mining and non-mining areas. It was found that the contents of 7 heavy metals (As, Cd, Pb, Zn, Cu, Sn, Sb) in E. oculatus of the mining area were higher than that in the non-mining 1-11 times. The analysis of morphology shows that the external morphology, the hind wing type and the chromosomal morphology of E. oculatus are significant differences between the two populations. Based on the heavy metal accumulation，morphological change, and stable population density, it is inferred that the mining area population has been affected by heavy metals and has adapted to the environment of heavy metals pollution. Then, by analyzing the transcriptome of the two populations, it was found that the digestion, immunity, excretion, endocrine, nerve, circulation, reproductive and other systems and lysosomes, endoplasmic reticulum and other cell structure-related gene expression were suppressed. This shows that the functions of the above-mentioned related systems of E. oculatus are inhibited by heavy metal stress. However, it has also been found that through the significant up-regulation of genes related to the above system, such as ATP2B, pepsin A, ubiquitin, AQP1, ACOX, ATPeV0A, SEC61A, CANX, ALDH7A1, DLD, aceE, Hsp40, and catalase, etc., and the down-regulation of MAPK signalling pathway genes, can enhanced nutrient absorption, improve energy metabolism, repair damaged cells and degrade abnormal proteins, maintain the stability of cells and systems, and resist heavy metal damage so that E. oculatus can adapt to the environment of heavy metal pollution for a long time.

Performances of a novel BAF with ferromanganese oxide modified biochar (FMBC) as the carriers for treating antibiotics, nitrogen and phosphorus in aquaculture wastewater.

In this paper, a biological aerated filter (BAF) based on ferromanganese oxide-biochar (FMBC) was constructed to investigated the removal performance and mechanism for conventional pollutants and four kinds of antibiotic, in contrast of conventional zeolite loaded BAF (BAF-A) and bamboo biochar filled BAF (BAF-B). Results showed that the average removal efficiency of total nitrogen (TN), total phosphorus (TP) and antibiotics in a FMBC-BAF (named by BAF-C) were 52.97 ± 2.27%, 51.58 ± 1.92% and 70.36 ± 1.00% ~ 81.65 ± 0.99% respectively in running period (39-100 d), which were significantly higher than those of BAF-A and BAF-B. In the BAF-C, the expression of denitrification enzyme activities and the secretion of extracellular polymeric substance (EPS) especially polyprotein (PN) were effectively stimulated, as well as accelerated electron transfer activity (ETSA) and lower electrochemical impedance spectroscopy (EIS) were acquired. After 100 days of operation, the abundance of nitrogen, phosphorus and antibiotic removal functional bacteria like Sphingorhabdus (4.52%), Bradyrhizobium (1.98%), Hyphomicrobium (2.49%), Ferruginibacter (7.80%), unclassified_f_Blastoca tellaceae (1.84%), norank_f_JG30-KF-CM45 (6.82%), norank_f_norank_o_SBR1031 (2.43%), Nitrospira (2.58%) norank_f_Caldilineaceae (1.53%) and Micropruina (1.11%) were enriched. Mechanism hypothesis of enhanced performances of nutrients and antibiotics removal pointed that: The phosphorus was removed by adsorption and precipitation, antibiotics removal was mainly achieved through the combined action of adsorption and biodegradation, while nitrogen removal was realized by biologic nitrification and denitrification in a FMBC-BAF for aquaculture wastewater treatment.

Complexity of influences on atrazine phytoremediation of coexisting graphene oxide in water: Mitigating its phytotoxicity while decreasing plant removal contribution.

Phytoremediation is an efficient technology for the removal of herbicide atrazine (ATZ) contamination in water bodies, but its ability to reduce ATZ under combined pollution remains unclear, especially ATZ co-existing with the emerging pollutant graphene oxide (GO) that may have potential effects on ATZ fate, plants and microbes. Herein, we investigated the phytoremediation potential of an emergent plant (Iris pseudacorus) for ATZ and the response of bacteria in a hydroponic system with and without GO. The results showed that plants enhanced ATZ dissipation in water with the increased removal rate by a factor of 1.7-4.0. GO restricted ATZ uptake by plants, but favored ATZ bioconcentration in cell walls. The plant contributed most to changes in the bacterial communities, decreasing the alpha diversity, while enriching the functional categories involving in amino acid and carbohydrate metabolisms. These findings indicated that I. pseudacorus can be employed as an effective candidate of phytoremediation for ATZ co-existing with GO at environmentally relevant concentrations, tending to recruit bacteria with plant stress tolerance and growth-promotion activities more than with ATZ degradation activities; GO exerted a mitigating effect on ATZ stress improving the barrier function of cell walls, but decreased the contribution of plants to ATZ removal.

Effects of soil metal(loid)s pollution on microbial activities and environmental risks in an abandoned chemical smelting site.

Abandoned chemical smelting sites containing toxic substances can seriously threaten and pose a risk to the surrounding ecological environment. Soil samples were collected from different depths (0 to 13 m) and analyzed for metal(loid)s content and fractionation, as well as microbial activities. The potential ecological risk indices for the different soil depths (ordered from high to low) were: 1 m (D-1) > surface (S-0) > 5 m (D-5) > 13 m (D-13) > 9 m (D-9), ranging between 1840.65-13,089.62, and representing extremely high environmental risks, of which Cd (and probably not arsenic) contributed to the highest environmental risk. A modified combined pollution risk index (MCR) combining total content and mobile proportion of metal(loid)s, and relative toxicities, was used to evaluate the degree of contamination and potential environmental risks. For the near-surface samples (S-0 and D-1 layers), the MCR considered that As, Cd, Pb, Sb, and Zn achieved high and alarming degrees of contamination, whereas Fe, Mn, and Ti were negligible or low to moderate pollution degrees. Combined microcalorimetry and enzymatic activity measurements of contaminated soil samples were used to assess the microbial metabolic activity characteristics. Correlation analysis elucidated the relationship between metal(loid)s exchangeable fraction or content and microbial activity characteristics (p < 0.05). The microbial metabolic activity in the D-1 layer was low presumably due to heavy metal stress. Enzyme activity indicators and microcalorimetric growth rate (k) measurements were considered sensitive indicators to reflect the soil microbial activities in abandoned chemical smelting sites.

How do different arsenic species affect the joint toxicity of perfluorooctanoic acid and arsenic to earthworm Eisenia fetida: A multi-biomarker approach.

Perfluorooctanoic acid (PFOA) and arsenic are widely distributed pollutants and can coexist in the environment. However, no study has been reported about the effects of different arsenic species on the joint toxicity of arsenic and PFOA to soil invertebrates. In this study, four arsenic species were selected, including arsenite (As(III)), arsenate (As(V)), monomethylarsonate (MMA), and dimethylarsinate (DMA). Earthworms Eisenia fetida were exposed to soils spiked with sublethal concentrations of PFOA, different arsenic species, and their binary mixtures for 56 days. The bioaccumulation and biotransformation of pollutants, as well as eight biomarkers in organisms, were assayed. The results indicated that the coexistence of PFOA and different arsenic species in soils could enhance the bioavailability of arsenic species while reducing the bioavailability of PFOA, and inhibit the arsenic biotransformation process in earthworms. Responses of most biomarkers in joint treatments of PFOA and As(III)/As(V) showed more significant variations compared with those in single treatments, indicating higher toxicity to the earthworms. The Integrated Biomarker Response (IBR) index was used to integrate the multi-biomarker responses, and the results also exhibited enhanced toxic effects in combined treatments of inorganic arsenic and PFOA. In comparison, both the biomarker variations and IBR values were lower in joint treatments of PFOA and MMA/DMA. Then the toxic interactions in the binary mixture systems were characterized by using a combined method of IBR and Effect Addition Index. The results revealed that the toxic interactions of the PFOA/arsenic mixture in earthworms depended on the different species of arsenic. The combined exposure of PFOA with inorganic arsenic led to a synergistic interaction, while that with organic arsenic resulted in an antagonistic response. Overall, this study provides new insights into the assessment of the joint toxicity of perfluoroalkyl substances and arsenic in soil ecosystems.

An Insight into the Combined Toxicity of 3,4-Dichloroaniline with Two-Dimensional Nanomaterials: From Classical Mixture Theory to Structure-Activity Relationship.

The assessment and prediction of the toxicity of engineered nanomaterials (NMs) present in mixtures is a challenging research issue. Herein, the toxicity of three advanced two-dimensional nanomaterials (TDNMs), in combination with an organic chemical (3,4-dichloroaniline, DCA) to two freshwater microalgae (Scenedesmus obliquus and Chlorella pyrenoidosa), was assessed and predicted not only from classical mixture theory but also from structure-activity relationships. The TDNMs included two layered double hydroxides (Mg-Al-LDH and Zn-Al-LDH) and a graphene nanoplatelet (GNP). The toxicity of DCA varied with the type and concentration of TDNMs, as well as the species. The combination of DCA and TDNMs exhibited additive, antagonistic, and synergistic effects. There is a linear relationship between the different levels (10, 50, and 90%) of effect concentrations and a Freundlich adsorption coefficient (KF) calculated by isotherm models and adsorption energy (Ea) obtained in molecular simulations, respectively. The prediction model incorporating both parameters KF and Ea had a higher predictive power for the combined toxicity than the classical mixture model. Our findings provide new insights for the development of strategies aimed at evaluating the ecotoxicological risk of NMs towards combined pollution situations.

Effects of cadmium and pyrene on earthworm-associated bacterial communities: Unveiling new perspectives for soil pollution management.

Earthworms are considered to be excellent bioindicators of soil pollution. In recent years, there has been increasing interest in examining the effects of soil pollution on earthworm-associated microbiomes, with a particular focus on the gut microbiomes. However, relatively little effort has been invested in comprehensively investigating other microbiomes associated with earthworms and their responses to soil pollution. To fill this gap, we systematically studied the effects of Cd, pyrene, and combined pollution on the bacterial community in different vermicompartments, i.e., burrow wall, gut, and cast, in both epigeic Eisenia fetida and anecic Metaphire guillelmi, using a 2D-terraria incubator and high-throughput sequencing techniques. The results showed that bacterial alpha diversity followed the order of burrow wall > cast > gut, and this did not vary with soil pollution or earthworm ecotypes. Moreover, the dominant phyla in the vermicompartments were similar across different pollution treatments. Principal coordinate analysis (PCoA) revealed that the bacterial communities in different vermicompartments and ecotypes of earthworm were separated from each other, whereas they were grouped together in polluted treatments and unpolluted conditions. These results imply that even in polluted soil, vermicompartment and earthworm ecotypes remain the most significant factors affecting earthworm-associated microbiomes. However, the impacts of soil pollution on the bacterial composition in each vermicompartment were still evident. A comprehensive analysis revealed that the gut bacterial communities are more sensitive to soil contamination than casts and burrow wall in different ecotypes. Additionally, linear discriminant analysis of effect size (LefSe) identified several bacteria in Gemmatimonadota, the Firmicutes phylum in the burrow walls, and Patescibacteria (phyla) in the gut as potential biomarkers for pyrene contamination in soil. This research provides a comprehensive understanding of the effects of soil pollution on earthworm-associated microbiomes, thereby enhancing our understanding of earthworm ecotoxicology and soil pollution management.

Adsorbed copper on urea modified activated biochar catalyzed H2O2 for oxidative degradation of sulfadiazine：Degradation mechanism and toxicity assessment.

The combined pollution of heavy metals and organic compounds usually occurs simultaneously and induces high toxicity. The technology of simultaneous removal of combined pollution is lacking and the removal mechanism is not clear. Sulfadiazine (SD), a widely used antibiotic, was used as a model contaminant. Urea modified sludge-based biochar (USBC) was prepared and used to catalyze H2O2 to remove the combined pollution of Cu2+ and sulfadiazine (SD) without causing secondary pollution. After 2 h, the removal rates of SD and Cu2+ were 100 and 64.8%, respectively. Cu2+ adsorbed on the surface of USBC accelerated the activation of H2O2 by the USBC catalyzed by CO bond to produce hydroxyl radical (•OH) and single oxygen (1O2) to degrade SD. Twenty-three intermediate products were detected, most of which were completely decomposed into CO2 and H2O. The toxicity was significantly reduced in the combined polluted system. This study highlights the potential of the low-cost technology based on sludge reuse and its inherent significance in reducing the toxic risk of combined pollution in the environment.

New insights into the effects of antibiotics and copper on microbial community diversity and carbon source utilization.

Residual antibiotics (ABs) and heavy metals (HMs) are continuously released from soil, reflecting their intensive use and contamination of water and soil, posing an environmental problem of great concern. Relatively few studies exist of the functional diversity of soil microorganisms under the combined action of ABs and HMs. To address this deficiency, BIOLOG ECO microplates and the Integrated Biological Responses version 2 (IBRv2) method were used to comprehensively explore the effects of single and combined actions of copper (Cu) and enrofloxacin (ENR), oxytetracycline (OTC), and sulfadimidine (SM2) on the soil microbial community. The results showed that the high concentration (0.80 mmol/kg) compound group had a significant effect on average well color development (AWCD) and OTC showed a dose-response relationship. The results of IBRv2 analysis showed that the single treatment group of ENR or SM2 had a significant effect on soil microbial communities, and the IBRv2 of E1 was 5.432. Microbes under ENR, SM2, and Cu stress had more types of available carbon sources, and all treatment groups were significantly more enriched with microorganisms having D-mannitol and L-asparagine as carbon sources. This study confirms that the combined effects of ABs and HMs can inhibit or promote the function of soil microbial communities. In addition, this paper will provide new insights into IBRv2 as an effective method to evaluate the impacts of contaminants on soil health.

Effects of combined antibiotics on nitrification, bacteria and antibiotic resistance genes in activated sludge: Insights from legacy effect of antibiotics.

The effect of combined antibiotics exposure on nitrogen removal, microbial community assembly and proliferation of antibiotics resistance genes (ARGs) is a hotspot in activated sludge system. However, it is unclear that how the historical antibiotic stress affects the subsequent responses of microbes and ARGs to combined antibiotics. In this study, the effects of combined sulfamethoxazole (SMX) and trimethoprim (TMP) pollution on activated sludge under legacy of SMX or TMP stress with different doses (0.005-30 mg/L) were investigated to clarify antibiotic legacy effects. Nitrification activity was inhibited under higher level of combined exposure but a high total nitrogen removal (∼70%) occurred. Based on the full-scale classification, the legacy effect of past antibiotic stress had a marked effect on community composition of conditionally abundant taxa (CAT) and conditionally rare or abundant taxa (CRAT). Rare taxa (RT) were the keystone taxa in the microbial network, and the responses of hub genera were also affected by the legacy of antibiotic stress. Nitrifying bacteria and genes were inhibited by the antibiotics and aerobic denitrifying bacteria (Pseudomonas, Thaurea and Hydrogenophaga) were enriched under legacy of high dose, as were the key denitrifying genes (napA, nirK and norB). Furthermore, the occurrences and co-selection relationship of 94 ARGs were affected by legacy effect. While, some shared hosts (eg., Citrobacter) and hub ARGs (eg., mdtD, mdtE and acrD) were identified. Overall, antibiotic legacy could affect responses of activated sludge to combined antibiotic and the legacy effect was stronger at higher exposure levels.

Remediation performance and mechanisms of Cu and Cd contaminated water and soil using Mn/Al-layered double oxide-loaded biochar.

The combined pollution of heavy metals is ubiquitous worldwide. Mn/Al-layered double oxide-loaded crab shells biochar (LDO/BC) was prepared, so as to remediate the combined pollution of Cd and Cu in soil and water. The pristine and used LDO/BC were characterized and the results revealed that the layered double oxide was successfully loaded on crab shells biochar (BC) and metal element Ca in crab shells was beneficial to the formation of more regular layered and flake structure. The maximal adsorption capacity (Qm) of LDO/BC for aqueous Cu2+ and Cd2+ was 66.23 and 73.47 mg/g, respectively. LDO/BC and BC were used to remediate e-waste-contaminated soil for the first time and exhibited highly efficient performance. The extraction amount of Cu and Cd in the contaminated soil by diethylene triamine penta-acetic acid (DTPA) after treating with 5% LDO/BC was significantly reduced from 819.84 to 205.95 mg/kg (with passivation rate 74.8%) and 8.46 to 4.16 mg/kg (with passivation rate 50.8%), respectively, inferring that the bioavailability of heavy metals declined remarkably. The experimental result also suggested that after remediation by LDO/BC the exchangeable and weak acid soluble Cu and Cd in soil translated to reducible, residual and oxidizable fraction which are more stable state. Precipitation, complexation and ion exchange were proposed as the possible mechanisms for Cd and Cu removal. In general, these experiment results indicate that LDO/BC can be a potentially effective reagent for remediation of heavy metal contaminated water and soil.

Co-exposure of Monensin Increased the Risks of Atrazine to Earthworms.

Antibiotics could enter farmlands through sewage irrigation or manure application, causing combined pollution with pesticides. Antibiotics may affect the environmental fate of pesticides and even increase their bioavailability. In this study, the influence of monensin on the degradation, toxicity, and availability of atrazine in soil-earthworm microcosms was investigated. Monensin inhibited the degradation of atrazine, changed the metabolite patterns in soil, and increased the bioavailability of atrazine in earthworms. Atrazine and monensin had a significant synergistic effect on earthworms in the acute toxic test. In long-term toxicity tests, co-exposure of atrazine and monensin also led to worse effects on earthworms including oxidative stress, energy metabolism disruption, and cocoon production compared to single exposure. The expression of tight junction proteins was down-regulated significantly by monensin, indicating that the intestinal barrier of earthworms was weakened, possibly causing the increased bioavailability of atrazine. The expressions of heat shock protein 70 (Hsp70) and reproductive and ontogenetic factors (ANN, TCTP) were all downregulated in binary exposure, indicating that the resilience and cocoon production of earthworms were further weakened under combined pollution. Monensin disturbed the energy metabolism and weakened the intestinal barrier of earthworms. These results showed that monensin increased the risks of atrazine in agricultural areas.

Low-dose cadmium affects the enantioselective bioaccumulation and dissipation of chiral penflufen in zebrafish (Danio rerio).

Pesticides are currently extensively used in agriculture, forestry, animal husbandry, and environmental hygiene, and their residues have become a global environmental problem, which can easily form combined pollution with heavy metals. The present study examined the effects of chronic (28 days) aqueous exposure of chiral penflufen (rac-penflufen, R-(-)-penflufen and S-(+)-penflufen), a widely used fungicide, with/without cadmium (Cd), a highly toxic heavy metal in zebrafish (Danio rerio). After rac-penflufen individual or combined exposure with Cd, the bioaccumulation and residual levels of S-(+)-penflufen were significantly higher than R-(-)-penflufen, and the effects of Cd were insignificantly. But for penflufen enantiomer, the effects of Cd were more serious for R-(-)-penflufen, which could increase the bioaccumulation (up to1.73 times), inhibit the dissipation (up to 32.3%) and enhance the residue (up to 5.35 times) of R-(-)-penflufen in zebrafish, decreasing the enantioselectivity. However, significant increase of S-(+)-penflufen concentrations was only found in viscera under co-exposure of Cd. The tissue distribution of penflufen enantiomers were not affected by the presence of Cd, and no interconversion of the two enantiomers occurred regardless of the presence of Cd. These findings indicated that co-contamination with Cd could increase the persistence of R-(-)-penflufen in zebrafish, thus increasing the environmental risks. The significant differences of Cd effects on chiral pesticide enantiomer and racemate indicated that the combined pollution of heavy metal and chiral pesticide might have enantiomer-specific, which should raise concern, and the enantioselective mechanism deserve further study.

Influence of cadmium and microplastics on physiological responses, ultrastructure and rhizosphere microbial community of duckweed.

The combined contamination of heavy metals and microplastics is widespread in freshwater environments. However, there are few researches on their combined effects on aquatic plants. In this study, the effects of single and combined stress of 0.01 mg L-1 cadmium (Cd), 50 mg L-1 polyethylene and 50 mg L-1 polypropylene for 15 days on the physiological response, ultrastructure and rhizosphere microbial community of duckweed were investigated. The results showed that Cd and microplastics single or combined stress inhibited the growth of duckweed, shortened the root length and decreased the chlorophyll content. Compared with single Cd treatments, the combination of microplastics and Cd increased duckweed growth rate and increased superoxide dismutase activity and malondialdehyde content and reduced chloroplast structural damage, indicating that the combined stress could reduce the toxicity of heavy metals to duckweed. Through the study of rhizosphere microbial diversity, 1381 Operational Taxonomic Unit (OTUs) were identified and rich microbial communities were detected in the duckweed rhizosphere. Among them, the main microbial communities were Proteobacteria, Bacteroidetes, and Cyanobacteria. Compared with Cd single stress, the ACE and chao index of rhizosphere microbial community increased under combined stress, indicating that the diversity and abundance of microbial communities were improved after combined stress treatment. Our study revealed the effects of heavy metals and microplastics on aquatic plants, providing a theoretical basis for duckweed applications in complex water pollution.

Predicting joint toxicity of chemicals by incorporating a weighted descriptor into a mixture model: Cases for binary antibiotics and binary nanoparticles.

A prediction method that integrated a mixture descriptor with an established mixture toxicology method was proposed for the joint toxicity of chemical pollutants. A weighted descriptor derived from the single descriptor of each component was employed to calculate a mixture descriptor, which was successfully embedded into the generalized concentration addition (GCA) model named the extended GCA (XGCA) model. To develop and validate the proposed approach, binary antibiotic mixtures (ciprofloxacin and oxytetracycline) and metal-oxide (copper oxide and zinc oxide) nanoparticle mixtures were selected to study their toxicity to freshwater green algae. The results showed that concentration-response curve (CRC) derived from the XGCA model was closer to the observed CRC than those from the GCA, Concentration Addition (CA), and Independent Action (IA) models. The difference between effect concentrations predicted by the XGCA model and observed did not exceed a factor of 1.6. The XGCA model was relatively more accurate at predicting joint toxicity (in terms of effect concentrations and effect errors) than the reference models, independent of component types and mixture ratios. The XGCA model predicts the joint toxicity through molecular structural or nanostructural characters, thus modes of toxic action are not preconditions for predicting the toxicity of the mixtures. This result demonstrates the practicability of using the XGCA method in toxicity assessments of mixture pollutants with unknown modes of action.