RESUMO
Polysaccharide-based hydrogels are achieving remarkable performances in chronic wounds treatment. In this work, a carboxymethyl cellulose-based hydrogel film was developed to support skin repair. The hydrogel was loaded with berberine, a polyphenolic molecule endowing antioxidant and cytoprotective features. The film was physico-chemically characterized and in vitro tested on keratinocytes and fibroblasts subjected to oxidative stress. The biocomposite showed high thermal stability (onset decomposition temperature 245 °C) and significant fluid uptake performances, both in free conditions (up to 6510%) and under external pressure (up to 3400%). Moreover, it was able to control oxidative stress and inflammation markers involved in wound chronicity. Keratinocytes hyperproliferation, features that normally hamper injury restoration, was reduced of 25%. Our results showed that the combination of berberine and hydrogel provides a synergic improvement of the material properties. The biocomposite represents a promising candidate for dermatological applications against oxidative stress at the chronic wound site, promoting the healing process.
Assuntos
Berberina/farmacologia , Carboximetilcelulose Sódica/química , Hidrogéis/farmacologia , Cicatrização/efeitos dos fármacos , Antioxidantes/farmacologia , Bandagens , Berberina/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Humanos , Hidrogéis/química , Queratinócitos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , TemperaturaRESUMO
Hydrolysates of arabinoxylan extracted from wheat chaff were prepared using different enzymatic treatments with an emphasis on improvements in their anti-diabetic, antioxidant and functional characteristics. The extracted arabinoxylan was subjected to enzymatic hydrolysis using individual xylanase, arabinofuranosidase, and feruloyl esterase, and their combinations. In all obtained hydrolysates, peaks corresponding to molecular weight lower than 38 kDa were noticed, while non-hydrolysed arabinoxylan had only peaks corresponding to 580 and 38 kDa. Results indicated that applied enzymes could hydrolyse polymeric arabinoxylan while their synergistic actions successfully modified its structure reflecting in lowered viscosity. Besides, it has been observed that the synergistic actions of enzymes improved the biological activities of arabinoxylan more than twice. Chemometric classification analysis showed that synergistic enzymes' actions were predominantly responsible for the improvement of biological activities. It indicated that they might be a useful tool for diversification and enhancement of biological activities of arabinoxylan from wheat chaff.
Assuntos
Hidrolases/metabolismo , Triticum/química , Xilanos/química , Xilanos/metabolismo , Hidrólise , Peso Molecular , Viscosidade , Xilanos/isolamento & purificação , Xilanos/farmacologiaRESUMO
This study aimed to prepare anthocyanin-rich microcapsules by spray and freeze-drying complex coacervated double emulsion using gelatin-acacia gum (GE-AG) and chitosan-carboxymethylcellulose (CS-CMC) and to investigate their properties and in vitro release kinetics. Microencapsulation efficiency (MEE) of the microcapsules varied from 84.9% to 94.7%. CS-CMS microcapsules showed significantly higher MEEs than those of GE-AG microcapsules. A significant higher MEE and lower moisture content and hygroscopicity was observed in spray-dried double emulsion (SDE) microcapsules. Freeze-dried double emulsion (FDE) microcapsules possessed higher total anthocyanin and total phenolic contents. The best fit for release kinetics was achieved using first-order and Higuchi models for SDE and FDE microcapsules, respectively. Diffusion-controlled release in the simulated gastric fluid was found for SDE microcapsules, while erosion-controlled release in simulated gastric and intestinal fluids predominated for FDE microcapsules. These findings suggest that the microcapsules can be applied for loading anthocyanins as a nutraceutical with controllable release requirement.
Assuntos
Antocianinas/química , Cápsulas , Carboximetilcelulose Sódica/química , Quitosana/química , Liberação Controlada de Fármacos , Emulsões , Liofilização , Goma Arábica/química , Cinética , Fenóis/químicaRESUMO
Bioactive compounds demonstrating antioxidant activity were analyzed in berries and leaves of nine cultivars of sea buckthorn (Hippophaë rhamnoides L.) of various ripening times. Total polyphenols were ranging between 0.70-3.62 g GAE.kg-1 (berries) and 1.88-3.72 g GAE.kg-1 (leaves). Leaves were significantly richer source of total flavonoids (14.40-49.44 mg RE.kg-1) in comparison with berries (0.55-4.11 mg RE.kg-1). Phenolic compounds, carotenoids and vitamins were determined using high-performance liquid chromatography with a diode array detection. The content of vitamin C was 0.98-3.65 g.kg-1 in berries and 22.81-46.32 g.kg-1 in leaves, vitamin E content was 6.98-29.91 g.kg-1 in berries and 71.54-153.99 g.kg-1 in leaves. Distribution of individual phenolic compounds varied, their total content in berries was considerably lower (76.1-205.2 mg.kg-1) than in leaves (1477.7-8709.0 mg.kg-1). Regarding antioxidant activity, Raisa and Slovan (berries) and Bojan and Maslicnaja (leaves) were evaluated as the best cultivars.
Assuntos
Antioxidantes/análise , Ácido Ascórbico/análise , Frutas/química , Hippophae/química , Folhas de Planta/química , Vitamina E/análise , Antioxidantes/química , Ácido Ascórbico/química , Carotenoides/análise , Cromatografia Líquida de Alta Pressão , República Tcheca , Flavonoides/análise , Frutas/fisiologia , Hippophae/fisiologia , Fenóis/análise , Fenóis/química , Polifenóis/análise , Vitamina E/químicaRESUMO
This study aimed to investigate the physicochemical properties, health-promoting benefits and rheological properties of an EPS produced by a novel probiotic Pediococcus pentosaceus M41 isolated from a marine source. P. pentosaceus M41 was able to produce an EPS with average molecular weight of 682.07â¯kDa. EPS-M41 consisted of arabinose, mannose, glucose and galactose with a molar ratio of 1.2:1.8:15.1:1.0. EPS-M41 structure could be proposed as â3)α-D-Glc(1â2)ß-D-Man(1â2)α-D-Glc(1â6)α-D-Glc(1â4)α-D-Glc(1â4)α-D-Gal(1â with arabinose linked at the terminals. At concentration of 10â¯mg.ml-1, the antioxidant capacity was 76.5% and 48.9% for DPPH and ABTS, respectively. EPS-M41 inhibited 86.8% and 90.8% of the α-amylase and α-glucosidase activities, respectively, at 100⯵g.ml-1. A 77.5% and 46.4% of antitumor inhibition occurred by EPS-M41 against Caco-2 and MCF-7 cells, respectively. The apparent viscosity (Æ) of all EPS-M41 solutions decreased with shear rate increases. Salt type and pH value had an impact on the rheological properties of EPS-M41.
Assuntos
Pediococcus pentosaceus/química , Polissacarídeos Bacterianos/farmacologia , Probióticos/química , Sequência de Carboidratos , Linhagem Celular Tumoral , Humanos , Peso Molecular , Polissacarídeos Bacterianos/química , Reologia , Substâncias Viscoelásticas/química , Substâncias Viscoelásticas/farmacologia , ViscosidadeRESUMO
Sorghum (Sorghum bicolor L. Moench) has been sparsely used as human food due to certain anti-nutritional factors such as tannins that reduce its digestibility, although the grain is an important source of bioactive compounds such as phenolic compounds (PCs). This study aimed to assess the impact of cooking and alkaline cooking (nixtamalization) on the bioaccessibility and antioxidant capacity of PCs of two sorghum varieties (white/red). Nixtamalization was the most effective procedure for the reduction of tannins (74.3%). Gallic acid proved to be the most bioaccessible PC (6359⯵g/g). The total phenolics and condensed tannins correlated with the antioxidant capacity (ABTS/DPPH; R2: 0.30-0.43, pâ¯<â¯0.05). These results confirm the potential of thermal procedures to significantly modify the bioaccessibility of sorghum compounds, enhancing their concentrations and reducing anti-nutritional factors (tannins) while improving their antioxidant capacity.
Assuntos
Antioxidantes/análise , Culinária , Grão Comestível/química , Fenóis/análise , Sorghum/química , Taninos/análise , Antioxidantes/metabolismo , Disponibilidade Biológica , Ácido Gálico/análise , Ácido Gálico/metabolismo , Humanos , Fenóis/metabolismoRESUMO
Vine Pruning residue was submitted to conventional heating and ohmic heating (OH) for the extraction of bioactive compounds and analyzed for total phenolic content (TPC), polyphenolic profile, antioxidant activity, antimicrobial activity and anticancer activity. The OH extracts were obtained using Low electric field (496.0 V/cm) or Intermediate electric field - IEF (840.0 V/cm). The tests were performed using 45% (v/v) ethanol-water extraction solution at 80 °C at different extraction times (20-90 min). The extract that stood out among the others concerning anticancer potential was the one obtained by OH when used, IEF, where the TPC was significantly higher than in the other extracts which correlated with higher antioxidant, antimicrobial and anti-proliferative activity on different tumor cell lines (HepG2, MDA-MB-231, MCF-7 and Caco2). Vine pruning OH extracts obtained using green solvents by an eco-friendly procedure were revealed as a source of compounds with relevant antioxidant and anticancer activity.
Assuntos
Antioxidantes/química , Extratos Vegetais/química , Polifenóis/química , Vitis/química , Antioxidantes/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Temperatura Alta , Humanos , Polifenóis/farmacologiaRESUMO
This study is an in-vitro investigation of the health-promoting properties of fermented whole-grain lupin, quinoa and wheat, using 72â¯h solid-state fermentation by Lactobacillus reuteri K777 and Lb. plantarum K779. Antiproliferative activity against Caco-2 and MCF-7 cancer cell lines (tumour cell lines of intestinal and mammary origin, respectively) was investigated, as well as α-amylase and α-glucosidase inhibition, antihypertensive, antioxidant and proteolytic activities. ABTS antioxidant activities of fermented lupin (FL, 55% w/v), quinoa (FQ, 55% w/v) and wheat (FW, 55% w/v) ranged from â¼12.0% to 55.0%, â¼17.6% to 73.0%, and â¼29.0% to 26.0%, respectively. Lupin, quinoa and wheat fermented by L. plantarum had pronounced antihypertensive activities (â¼85%). The α-glucosidase inhibition in FL was higher than that of FQ and FW. The magnitude of the antiproliferative activities of FL was markedly greater (pâ¯<â¯0.05) than of FQ and FW by approximately three-fold and two-folds against Caco-2 and MCF-7 cell lines, respectively.
Assuntos
Chenopodium quinoa/microbiologia , Lactobacillus/fisiologia , Lupinus/microbiologia , Probióticos/farmacologia , Triticum/microbiologia , Anti-Hipertensivos/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/farmacologia , Células CACO-2 , Fermentação , Inibidores de Glicosídeo Hidrolases/farmacologia , Humanos , Células MCF-7 , alfa-Amilases/antagonistas & inibidoresRESUMO
Berries of four gooseberry (Ribes uva-crispa L.) cultivars of Invicta, Rixanta, Karat and Black Negus and five currant (Ribes L.) cultivars of NS 11, Focus, Ben Gairn, Otelo and Viola were evaluated as potential sources of bioactive compounds with extraordinary antioxidant activity. Their total phenolic, flavonoid and anthocyanin contents were determined in the range of 3.52-30.77â¯gâ¯GA.kg-1, 2.83-17.35â¯gâ¯RE.kg-1 and 0.03-186.12â¯mgâ¯COG.100â¯g-1, respectively. Furthermore, quantification of phenolic compounds and vitamins was established by high-performance liquid chromatography-diode array detection. Flavonoids were the most abundant phenolic substances in the range of 345.0-3726.5â¯mg.kg-1. Ascorbic acid and vitamin E were established in the amounts of 6.2-14.04â¯g.kg-1 and 0.43-12.85â¯mg.kg-1, respectively. Considering all analyzed factors and antioxidant activities determined by various methods (DPPH, ACW and ACL), red gooseberry Black Negus and black currant Otelo were the most significant cultivars.
Assuntos
Antioxidantes/química , Ácido Ascórbico/análise , Cromatografia Líquida de Alta Pressão , Fenóis/análise , Ribes/química , Vitamina E/análise , Antocianinas/análise , Flavonoides/análise , Frutas/química , Frutas/metabolismo , Ribes/metabolismoRESUMO
The aim of this study was to investigate the antioxidant, mechanical and physical properties of a new film-based polymeric blend of hydrocolloids obtained from the aqueous extraction of chia seeds (source antioxidants) and apple pectin. The individual matrices films were brittle and rigid with poor mechanical properties. The blends formulations contributed to improved mechanical properties regarding workability and resistance. The antioxidant results showed the potential hydrocolloid from chia seeds as natural source of antioxidant in these polymeric films. The formulation 3 (14(hydrocolloid):41(pectin):25(glycerol):20(glutaraldehyde) displayed well thermal, mechanical, morphological and antioxidant properties, suggesting their great potential for food packaging.
RESUMO
Olives and olive oil, a key food type of the Mediterranean diets, are packed with various important polyphenols including oleuropein (OLE), hydroxytyrosol (HTY) and tyrosol (TYR). OLE and HTY are highly powerful antioxidants and play a prime role in the therapeutics of free radical-related diseases. Their molecular stabilities and antioxidant properties can be improved by cyclodextrin (CD) encapsulation. Here, we present a systematic investigation on the inclusion complexes of ß-CD-TYR (1), ß-CD-HTY (2) and ß-CD-OLE (3) by combined single-crystal structure determination, DFT complete-geometry optimization and DPPH antioxidant assay. X-ray analysis and DFT calculation reveal the preference of inclusion geometry with deep protrusion of the aromatic ring moieties of TYR, HTY and OLE from the ß-CD O6-H-side, and the common host-guest stabilization scheme via intermolecular O-Hâ¯O hydrogen bonding interactions. No polyphenol OH group is shielded in the ß-CD cavity, in contrast to the structures of ß-CD-tea catechins complexes. The established host-guest O-Hâ¯O hydrogen bonds help to elevate antioxidant capacities of the olive polyphenols upon ß-CD encapsulation. The order of antioxidant activity 2 >3 â« 1 based on the DPPH measurement is in fair agreement with their relative thermodynamic stabilities derived from DFT calculation.
Assuntos
Sequestradores de Radicais Livres/química , Iridoides/química , Álcool Feniletílico/análogos & derivados , beta-Ciclodextrinas/química , Cristalografia por Raios X , Ligação de Hidrogênio , Glucosídeos Iridoides , Modelos Químicos , Estrutura Molecular , Álcool Feniletílico/química , Teoria Quântica , Relação Estrutura-Atividade , TermodinâmicaRESUMO
This study aimed to investigate in vitro the health-promoting benefits (anticancer activity, α-amylase and α-glucosidase inhibition, angiotensin-converting-enzyme (ACE)-inhibition, antioxidant and proteolytic activity) of camel milk fermented with indigenous probiotic strains of Lactobacillus spp., compared with fermented bovine milk. The three camel milk probiotic strains Lb. reuteri-KX881777, Lb. plantarum-KX881772, Lb. plantarum-KX881779 and a control strain Lb. plantarum DSM2468 were employed to ferment camel and bovine milks separately. The proteolytic and antioxidant activity of water soluble extracts (WSEs) from all fermented camel milks were higher than those of fermented bovine milk. α-Amylase inhibition of WSEs were >34% in both milk types fermented with all strains during storage periods, except the WSE of camel milk fermented by Lp.K772. The highest ACE-inhibition of the WSE from camel milk fermented by Lr.K777 was >80%. The proliferations of Caco-2, MCF-7 and HELA cells were more inhibited when treated with the WSE of fermented camel milk.
Assuntos
Camelus , Animais , Antioxidantes , Células CACO-2 , Fermentação , Humanos , Leite , Probióticos , alfa-Amilases , alfa-GlucosidasesRESUMO
The goal of this study was to improve the chemical stability of menhaden oil and control the lipolysis in emulsions with whey protein during in vitro digestion through EGCG conjugation and genipin-mediated interfacial cross-linking (CL). WPI-EGCG conjugate was successfully synthesized, confirmed by SDS-PAGE, ESI-MS, and phenolic group quantifications (125.3â¯mg/g), and characterized with far UV CD and ATR-FTIR. Emulsion particle diameter with WPI-EGCG is lower than with WPI. Compared to the native emulsion, WPI CL increased particle diameter and physical stability. Higher oxidative stability was observed for emulsions stabilized with WPI-EGCG conjugate than that with interfacial cross-linking due to the great antioxidant activity. Whereas, WPI CL is more effective than WPI-EGCG conjugate in hindering the rate and extent of lipolysis. The combination of EGCG conjugation and interfacial CL showed both the highest protection of menhaden oil against degradation and highest inhibition on the rate and extent of lipolysis of menhaden oil.
Assuntos
Emulsões/química , Óleos de Peixe/química , Proteínas do Soro do Leite/química , Antioxidantes/química , Catequina/análogos & derivados , Catequina/química , Dicroísmo Circular , Digestão , Eletroforese em Gel de Poliacrilamida , Emulsões/metabolismo , Iridoides/química , Lipólise , Oxirredução , Espectrometria de Massas por Ionização por Electrospray , Espectroscopia de Infravermelho com Transformada de Fourier/métodosRESUMO
Crataegus dahurica Koehne is an edible wild fruit mainly distributed in Northeast China. The purpose of this study was to elucidate the chemical constituents and investigate the bioactivities of dried fruit of C. dahurica methanol extract (CdME). Through various chromatographic methods, thirty-five compounds were isolated from CdME for the first time and their structures were identified on the basis of physicochemical properties and spectroscopic data. The main structural types of these compounds were triterpenoids and polyphenolics. Pharmacological experiments results showed that CdME had potently antioxidant capacity and ethyl acetate fraction was the active part with the greatest antioxidant activities. Moreover, CdME especially n-butanol fraction significantly accelerated the gastrointestinal transit in mice (acceleration rate: 78.5⯱â¯1.5% vs. 69.9⯱â¯3.2% at a dose of 250â¯mg/kg, compared to the control group, Pâ¯<â¯.01). On the basis of these results, C. dahurica may be considered as a good resource of antioxidants and digestion-improving agents.
Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Crataegus/química , Trânsito Gastrointestinal , Animais , China , Frutas/química , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Camundongos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Triterpenos/análiseRESUMO
Polyphenolic catechins prevalent in tea are powerful antioxidants for therapeutics of various free radical-related diseases. The non-epicatechins are thermally obtained from the naturally abundant epicatechins. In our study series on the structure-antioxidant property relationship of the CD inclusion complexes with tea catechins, this closing chapter presents the ß-CD encapsulation of three non-epicatechins, i.e., (-)-gallocatechin (GC) 1, (-)-catechin gallate (GC) 2, and (-)-gallocatechin gallate (GCG) 3 investigated by means of single-crystal X-ray diffraction, DFT calculation and DPPH radical scavenging activity assay. Detailed structural comparisons of the ß-CD inclusion complexes with both non-epi and epi type catechins reveal that a common host-guest hydrogen bonding scheme and the shielding of catechin OH groups inside the host circular wall play a prime role in flourishing antioxidant capacities in the order of 3â¯>â¯2â¯>â¯1. This is consistent with the relative thermodynamic stabilities derived from DFT energy minimization.
Assuntos
Antioxidantes/química , Compostos de Bifenilo/análise , Picratos/análise , Teoria Quântica , Chá/química , Raios X , beta-Ciclodextrinas/química , Estrutura MolecularRESUMO
The aim of this work was to study the effect of heat and moisture treatment (HMT) on carotenoids, phenolic content and antioxidant capacity of ground, orange maize. Total carotenoid content (TCC) of untreated sample (53.39â¯mg/kg) was 2.2 times higher than measured in treated orange maize f (24.61â¯mg/kg). The rates of degradation with HMT were in the following order: ß-caroteneâ¯>â¯ß-cryptoxanthinâ¯>â¯zeaxanthinâ¯>â¯lutein. There was a significant interaction between longer heating time and higher moisture content on carotenoid degradation (pâ¯<â¯.05). Total phenolic content (TPC) in raw sample (1664.74â¯mg/kg) was two-fold higher than in treated orange maize (827.89â¯mg/kg). Ferulic acid was the most abundant and stable phenolic acid in raw and treated orange maize. The antioxidant capacity of orange maize was higher in methanol than in butanol extracts. The highest correlation (0.924) was observed between TPC and ABTS+ scavenging capacity of methanol extracts.
Assuntos
Antioxidantes/análise , Carotenoides/análise , Farinha/análise , Manipulação de Alimentos/métodos , Zea mays/química , Antioxidantes/química , Carotenoides/química , Temperatura Alta , Luteína/análise , Fenóis/análise , Zeaxantinas/análiseRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Thapsia garganica, is a herbal medicine traditionally used as diuretic, emetic and purgative. It is also used as anti-scorpion venom in Morocco; however, its protective effects against scorpion venom remain elusive. AIM OF THE STUDY: The present study was undertaken to evaluate anti-venom activity of T. garganica in vivo through histological and biochemical studies. MATERIALS AND METHODS: Methanolic leaves extract of T. garganica was evaluated for anti-venom activity against buthus. occitanus under in vivo conditions. Histopathological and biochemical changes in envenomed and treated mice were also examined. Phytochemical screening was conducted to estimate the major constituents whereas DPPH, ß -Carotene-linoleic acid and reducing power assays were performed to evaluate the anti-oxidant activity of T. garganica extract. RESULTS: Methanolic leaves extract of T. garganica (2g/kg) increased the survival time (> 18h) of mice injected with lethal doses of B. occitanus venom, with remarkable recovery of histology damage. Furthermore T. garganica induced a significant decreased of biochemical markers of kidney, liver and heart function. Phytochemistry screening revealed the presence of phenolic compounds, flavonoids, tannins and steroids/terpenoids, which might explain the bioactivity of the extract. It was also shown that the extract has an exceptionally high antioxidant activity compared to well-known antioxidants used as standards. CONCLUSION: The present study provides strong evidence that support the use of T. garganica as anti-scorpion venom in traditional medicine in Morocco. However, additional studies are required to isolate and identify the metabolites responsible for the activity.
Assuntos
Antioxidantes/uso terapêutico , Antivenenos/uso terapêutico , Extratos Vegetais/uso terapêutico , Venenos de Escorpião/toxicidade , Thapsia , Animais , Antioxidantes/análise , Antioxidantes/toxicidade , Antivenenos/análise , Antivenenos/toxicidade , Rim/efeitos dos fármacos , Rim/patologia , Dose Letal Mediana , Fígado/efeitos dos fármacos , Fígado/patologia , Pulmão/efeitos dos fármacos , Pulmão/patologia , Masculino , Medicina Tradicional , Metanol/química , Camundongos , Marrocos , Miocárdio/patologia , Extratos Vegetais/análise , Extratos Vegetais/toxicidade , Folhas de Planta , Solventes/químicaRESUMO
Novel bacterial cellulose (BC) based monolayer and multilayer films, incorporating 5wt.% Scrophularia striata Boiss. extract (SE) were obtained. The effect of lamination and ß-cyclodextrin (ß-CD) inclusion complexation of SE on morphological, physical, antioxidant and release properties of films were investigated. FT-IR results reflected that some new interactions have occurred between BC and ß-CD. The XRD analyses showed a decrease in diffraction intensities of BC by addition of free SE. SEM results indicated that the intrinsic compactness of the BC film was preserved by addition of SE/ß-CD complex. Lamination and SE/ß-CD addition enhanced the mechanical properties. SE loaded films exhibited a good antioxidant activity. Release studies indicated that the release rate and diffusion coefficient (D) of SE in 95% ethanol simulant were significantly decreased by lamination and complexation of SE with ß-CD. Results suggest that SE loaded BC films may be used as controlled release antioxidant food active packaging.
Assuntos
Antioxidantes/química , Celulose/química , Embalagem de Alimentos , Extratos Vegetais/química , Scrophularia/química , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
In this work, a novel polysaccharide (named DBFM3) was isolated from mycelia of Bjerkandera fumosa by DEAE-32 and Sepharose CL-6B column chromatography. High-performance gel permeation chromatography showed that DBFM3 was homogeneous, with an average molecular weight of 1.8×105Da. Structural characteristics of the purified fraction were investigated by high-performance liquid chromatography (HPLC), FT-IR, and NMR. HPLC analysis indicated that DBFM3 was composed of mannose, galacturonic acid, and galactose at molar ratios of 1:18.16:0.702. Spectral analysis suggested that DBFM3 had (1â6), (1â3,6), (1â3) linkages and pyranose conformation. Antioxidant assay in vitro showed that DBFM3 exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activity, and has protective effect against DNA damage and damage to SH-SY5Y cells induced by H2O2. Immunological tests indicated that DBFM3 significantly increased lymphocyte proliferation in vitro. Furthermore, DBFM3 increased the proliferation of lymphocytes in the presence of concanavalin A or lipopolysaccharide as mitogen.
Assuntos
Coriolaceae/química , Polissacarídeos Fúngicos/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Fermentação , Sequestradores de Radicais Livres/farmacologia , Polissacarídeos Fúngicos/isolamento & purificação , Humanos , Peróxido de Hidrogênio , Linfócitos/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Heracleum dissectum Ledeb. has long been used as a wild edible vegetable by local people in China. The purpose of this study is to investigate the antidiabetic potential of aerial part of H. dissectum methanol extract (HdME) and the chemical constituents. Ten compounds including eight coumarins were isolated and four of them were found from H. dissectum for the first time. HdME potently inhibited the elevation of plasma glucose after its oral administration to glucose-loaded mice, and its petroleum ether (PE) fraction exerted the greatest inhibitory activities. Meanwhile, HdME (125 and 250mg/kg) also significantly decreased the blood glucose level in STZ-induced diabetic mice, but had no effect in normoglycemic mice. Additionally, HdME showed weak inhibitory effects on α-glucosidase activity and DPPH free radicals scavenging. In conclusion, HdME has antidiabetic action and PE fraction is the active part where coumarins possibly play an important role in antidiabetic activity.