Bulk and Mapping Speciation Analyses Unveil the Pattern and Heterogeneity of Cu Species during Organic Waste Treatment.

Organic wastes (OWs) can be a common source of copper (Cu) contamination of agricultural soils. Here we conducted a comprehensive study of 22 raw and treated OWs sampled at 6 different full-scale OW treatment plants. Bulk XANES analysis findings indicated that the Cu oxidation state was subject to changes throughout the OW treatment process, mostly depending on the anaerobic/aerobic conditions prevailing in each treatment stage. These changes were independent of the OW origin (agricultural, urban or industrial). Cu(I) prevailed in raw OWs and digestates (88-100%), whereas Cu(II) dominated in composts (46-100%). Bulk EXAFS analysis confirmed these observations and revealed that Cu(I) species in raw OWs and digestates consisted mainly of Cu(I)-sulfide (76-100%), while Cu(II) species (60-100%) in composts were Cu(II)-citrate, Cu(II)-carbonate and amorphous Cu(II)-phosphate. Interestingly, we observed that anaerobic digestion was conducive to the formation of crystallized Cu(I)-sulfides at the expense of nanosized and poorly crystalline Cu(I)-sulfide species, and that the recalcitrant Cu(I) species in composts was always crystallized Cu(I)-sulfide. XANES imaging analysis revealed Cu(II) species present in low proportions (2-4%) that were not detected using bulk XAS analysis in raw OWs and digestates. This demonstrated the potential of XANES imaging for probing minor species in complex matrices.

Sustainable strategies: Nature-based solutions to tackle antibiotic resistance gene proliferation and improve agricultural productivity and soil quality.

The issue of antibiotic resistance is now recognized by the World Health Organisation (WHO) as one of the major problems in human health. Although its effects are evident in the healthcare settings, the root cause should be traced back to the One Health link, extending from animals to the environment. In fact, the use of organic fertilizers in agroecosystems represents one, if not the primary, cause of the introduction of antibiotics and antibiotic-resistant bacteria into the soil. Since the concentrations of antibiotics introduced into the soil are residual, the agroecosystem has become a perfect environment for the selection and proliferation of antibiotic resistance genes (ARGs). The continuous influx of these emerging contaminants (i.e., antibiotics) into the agroecosystem results in the selection and accumulation of ARGs in soil bacteria, occasionally giving rise to multi-resistant bacteria. These bacteria may harbour ARGs related to various antibiotics on their plasmids. In this context, these bacteria can potentially enter the human sphere when individuals consume food from contaminated agroecosystems, leading to the acquisition of multi-resistant bacteria. Once introduced into the nosocomial environment, these bacteria pose a significant threat to human health. In this review, we analyse how the use of digestate as an organic fertilizer can mitigate the spread of ARGs in agroecosystems. Furthermore, we highlight how, according to European guidelines, digestate can be considered a Nature-Based Solution (NBS). This NBS not only has the ability to mitigate the spread of ARGs in agroecosystems but also offers the opportunity to further improve Microbial-Based Solutions (MBS), with the aim of enhancing soil quality and productivity.

Digestate induces significantly higher N2O emission compared to urea under different soil properties and moisture.

Digestate is considered as an option for recycling resources and a part of the substitution for chemical fertilizers to reduce environmental impacts. However, its application may lead to significant nitrous oxide (N2O) emissions because of its high concentration of ammonium and degradable carbon. The research objectives are to evaluate how N2O emissions respond to digestate as compared to urea application and whether this depends on soil properties and moisture. Either digestate or urea (100 mg N kg-1) was applied with and without a nitrification inhibitor of 3,4-dimethylpyrazole phosphate (DMPP) to three soil types (fluvo-aquic soil, black soil, and latosol) under three different soil moisture conditions (45, 65, and 85% water-filled pore space (WFPS)) through microcosm incubations. Results showed that digestate- and urea-induced N2O emissions increased exponentially with soil moisture in the three studied soils, and the magnitude of the increase was much greater in the alkaline fluvo-aquic soil, coinciding with high net nitrification rate and transient nitrite accumulation. Compared with urea-amended soils, digestate led to significantly higher peaks in N2O and carbon dioxide (CO2) emissions, which might be due to stimulated rapid oxygen consumption and mineralized N supply. Digestate-induced N2O emissions were all more than one time higher than those induced by urea at the three moisture levels in the three studied soils, except at 85% WFPS in the fluvo-aquic soil. DMPP was more effective at mitigating N2O emissions (inhibitory efficacy: 73%-99%) in wetter digestate-fertilized soils. Overall, our study shows the contrasting effect of digestate to urea on N2O emissions under different soil properties and moisture levels. This is of particular value for determining the optimum of applying digestate under varying soil moisture conditions to minimize stimulated N2O emissions in specific soil properties.

Nutrient recovery from anaerobic digestate: Fertilizer informatics for circular economy.

This study explores the integration of fertilizer informatics into the circular economy, with a focus on enhancing nutrient recovery from anaerobic digestate. It utilizes advanced algorithms and data analytics to develop new nutrient management strategies essential for sustainable agriculture. This research provides a detailed assessment of current nutrient recovery technologies, evaluating their environmental impact, cost efficiency, and adaptability. Our findings highlight the importance of merging circular economy principles with fertilizer informatics, showcasing the potential for transforming waste into environmentally friendly fertilizers. This approach has significant implications for improving agricultural practices towards sustainability. The methodologies and insights presented are relevant for ongoing research in environmental stewardship and sustainable resource management. This study describes practical solutions and new perspectives, making it a valuable reference for future research.

Enhanced dewaterability of food waste digestate by biochar/potassium ferrate treatments: Performance and mechanisms.

The combined process of biochar (BC) and potassium ferrate (PF) offers a fascinating technique for efficient dewatering of digestate. However, the effects of BC/PF treatment on the dewaterability and mechanisms of FWD are still unknown. This study aimed to reveal the impact mechanisms of BC/PF treatment on digestate dewatering performance. Experimental results indicated that BC/PF treatment significantly enhanced the dewaterability of digestate, with the minimum specific resistance to filtration of (1.05 ± 0.02) × 1015 m·kg-1 and water content of 57.52 ± 0.51% being obtained at the concentrations of 0.018 g·g-1 total solid (TS) BC300 and 0.20 g·g-1 TS PF, which were 8.60% and 13.59% lower than PF treatment, respectively. BC/PF treatment proficiently reduced the fractal dimension, bound water content, apparent viscosity, and gel-like network structure strength of digestate, as well as increased the floc size and zeta potential of digestate. BC/PF treatment promoted the conversion of extracellular polymeric substances (EPS) fractions from inner EPS to soluble EPS, increased the fluorescence intensity of the dissolved compounds, and enhanced the hydrophobicity of proteins. Mechanisms investigations showed that BC/PF enhanced dewatering through non-reactive oxygen species pathways, i.e., via strong oxidative intermediate irons species Fe(V)/Fe(IV). BC/PF treatment enhanced the solubilization of nutrients, the inactivation of fecal coliforms, and the mitigation of heavy metal toxicity. The results suggested that BC/PF treatment is an effective digestate dewatering technology which can provide technological supports to the closed-loop treatment of FWD.

Vermifiltration as a green solution to promote digestate reuse in agriculture in small-scale farms.

Digestates from low-tech digesters need to be post-treated to ensure their safe agricultural reuse. This study evaluated, for the first time, vermifiltration as a post-treatment for the digestate from a low-tech digester implemented in a small-scale farm, treating cattle manure and cheese whey under psychrophilic conditions. Vermifiltration performance was monitored in terms of solids, organic matter, nutrients, and pathogens removal efficiency. In addition, the growth of earthworms (Eisenia foetida) and their role in the process was evaluated. Finally, the vermicompost and the effluent of the vermifilter were characterized in order to assess their potential reuse in agriculture. Vermifilters showed high removal efficiency of chemical oxygen demand (55-90%), total solids (60-80%), ammonium nitrogen (83-97%), and phosphate-P (28-49%). Earthworms effectively grew and reproduced on digestate (i.e. earthworms number increased by 183%), enhancing the vermifiltration performance, while reducing clogging and odour-related issues. Both the vermicompost and effluent produced complied with legislation limits established for soil improvers and wastewater for fertigation, respectively. Indeed, there was an absence of pathogens and non-detectable heavy metals concentrations. Vermifiltration may be thus considered a suitable post-treatment option for the digestate from low-tech digesters, allowing for its safe agricultural reuse and boosting the circular bioeconomy in small-scale farms.

Multi-stage aeration regime to regulate organic conversion toward gas alleviation and humification in food waste digestate composting.

Aerobic composting has been considered as a pragmatic technique to convert food waste digestate into high-quality biofertiliser. Nevertheless, massive gaseous emission and immature product remain the primary challenges in food waste digestate composting. Thus, the performance of multi-stage aeration regimes to improve gaseous emissions and organic humification during food waste digestate composting was investigated in this study. In addition to continuous aeration with a constant intensity of 0.3 L kg·dry mass (DM)-1·min-1, two multi-stage decreased aeration regimes were designed as "0.3-0.2-0.1" and "0.3-0.1-0.1" L·kg·DM-1·min-1 from the thermophilic to cooling and then mature stages, respectively. Results showed that the decreased aeration regimes could alleviate nitrous oxide (N2O) and ammonia (NH3) emission and slightly enhance humification during composting. The alleviated N2O and NH3 emission were mainly contributed by abiotically reducing gaseous release potential as well as biotically inactivating denitrifers (Pusillimonas and Pseudidiomarina) and proliferating Atopobium to reduce nitrate availability under lower aeration supply. The "0.3-0.2-0.1 L kg·DM-1·min-1" regime exhibited a more excellent performance to alleviate N2O and NH3 emission by 27.5% and 16.3%, respectively. Moreover, the decreased aeration regimes also favored the enrichment of functional bacteria (Caldicoprobacter and Syntrophomonas) to accelerate lignocellulosic biodegradation and thus humic acid synthesis by 6.5%-11.2%. Given its better performance to improve gaseous emissions and humification, the aeration regime of "0.3-0.2-0.1 L kg·DM-1·min-1" are recommended in food waste digestate composting in practice.

Targeted regulation of digestate dewaterability by the ozone/persulfate oxidation process.

Dewatering is the first step in the subsequent treatment and disposal of food waste digestate (FWD). However, FWD is difficult to dewatering. In this study, persulfate was synergistic oxidized by ozone to improve digestate dewaterability. The optimal conditions was at pH = 3, O3＝40 mg/g TS and PDS＝0.1 g/g TS, under which the reductions in the normalized capillary suction time (NCST) and bound moisture (BM) of the FWD were 89.97% and 65.79%, respectively. Hydrophilic functional groups (oxygen- and nitrogen-containing groups) and hydrophilic protein molecular structures were decomposed by the reactive species of sulfate radical (SO4·-) and hydroxyl radicals (·OH) generated in the ozone-persulfate oxidation process, disrupting the binding between EPS and water molecules. The contributions of SO4·- and ·OH to digestate dewaterability were 42.51% and 28.55%. In addition, the introduction of H+ reduced electrostatic repulsion and contributed to the condensation of digestate flocs. The environmental implication assessment and economic analysis suggested that the O3/PDS oxidation process was cost-effective and has a low environmental implication when applied to the FWD dewaterability improvement process. These results can serve as a reference for the management of FWD and further improvement of FWD treatment and disposal efficiency.

Solid-liquid separation of digestate from biogas plants: A systematic review of the techniques' performance.

Digestate processing is a strategy to improve the management of digestate from biogas plants. Solid-liquid separation is usually the primary step and can be followed by advanced treatments of the fractions. The knowledge about the performance of the separators and the quality of the fractions is scattered because of many available techniques and large variability in digestate characteristics. We performed a systematic review and found 175 observations of full-scale solid-liquid separation of digestate. We identified 4 separator groups, 4 digestate classes based on substrate, and distinguished whether chemical conditioners were used. We confirmed the hypothesis that the dominant substrate can affect the efficiency of the digestate separation. Furthermore, the results showed that centrifuges separated significantly more dry matter and total P than screw presses. Use of chemical conditioners in combination with a centrifuge lowered the dry matter concentration in the liquid fraction by 30%. Screw presses consumed 4.5 times less energy than centrifuges and delivered 3.3 tonne ammonium N in the liquid fraction and 0.3 tonne total P in the solid fraction using 1 MWh. The results can provide data for systems analyses of biogas solutions and can support practitioners when choosing among full-scale separator techniques depending on the digestate type. In a broader perspective, this work contributes to the continuous improvement of biogas plants operations and to their role as nutrients recovery sites.

Cultivation of yeasts on liquid digestate to remove organic pollutants and nutrients and for potential application as co-culture with microalgae.

In this study, the growth of yeast and yeast-like fungi in the liquid digestate from vegetable wastes was investigated in order to remove nutrients and organic pollutants, and for their application as co-culture members with green microalgae. The studied yeast strains were characterized for their assimilative and enzymatic profiles as well as temperature requirements. In the first experimental stage, the growth dynamics of each strain were determined, allowing to select the best yeasts for further studies. In the subsequent stage, the ability of selectants to remove organic pollutants was assessed. Different cultivation media containing respectively 1:3, 1:1, 3:1 vol ratio of liquid digestate and the basal minimal medium were used. Among all tested yeast strains, Rhodotorula mucilaginosa DSM 70825 showed the most promising results, demonstrating the highest potential for removing organic substrates and nutrients. Depending on the medium, this strain achieved 50-80% sCOD, 45-60% tVFAs, 21-45% TN, 33-52% PO43- reduction rates. Similar results were obtained for the strain Candida sp. OR687571. The high nutrient and organics removal efficiency by these yeasts could likely be linked to their ability to assimilate xylose (being the main source of carbon in the liquid digestate). In culture media containing liquid digestate, both yeast strains achieved good viability and proliferation potential. In the liquid digestate medium, R. mucilaginosa and Candida sp. showed vitality at the level of 51.5% and 45.0%, respectively. These strains seem to be a good starting material for developing effective digestate treatment strategies involving monocultures and/or consortia with other yeasts or green microalgae.

Synergetic anaerobic digestion of food waste for enhanced production of biogas and value-added products: strategies, challenges, and techno-economic analysis.

The generation of food waste (FW) is increasing at an alarming rate, contributing to a total of 32% of all the waste produced globally. Anaerobic digestion (AD) is an effective method for dealing with organic wastes of various compositions, like FW. Waste valorization into value-added products has increased due to the conversion of FW into biogas using AD technology. A variety of pathways are adopted by microbes to avoid unfavorable conditions in AD, including competition between sulfate-reducing bacteria and methane (CH4)-forming bacteria. Anaerobic bacteria decompose organic matter to produce biogas, a digester gas. The composition depends on the type of raw material and the method by which the digestion process is conducted. Studies have shown that the biogas produced by AD contains 65-75% CH4 and 35-45% carbon dioxide (CO2). Methanothrix soehngenii and Methanosaeta concilii are examples of species that convert acetate to CH4 and CO2. Methanobacterium bryantii, Methanobacterium thermoautotrophicum, and Methanobrevibacter arboriphilus are examples of species that produce CH4 from hydrogen and CO2. Methanobacterium formicicum, Methanobrevibacter smithii, and Methanococcus voltae are examples of species that consume formate, hydrogen, and CO2 and produce CH4. The popularity of AD has increased for the development of biorefinery because it is seen as a more environmentally acceptable alternative in comparison to physico-chemical techniques for resource and energy recovery. The review examines the possibility of using accessible FW to produce important value-added products such as organic acids (acetate/butyrate), biopolymers, and other essential value-added products.

HighlightsPopulation growth globally increases the generation of FW.FW generation, recycling, and reuse have been discussed.Biogas and bio-fertilizers can be recovered from FW through AD.

Phosphorus Recovery from Whole Digestate through Electrochemical Leaching and Precipitation.

Phosphorus (P) recovery from biosolids can play an important role in a circular economy. Herein, an electrochemical phosphorus recovery cell (EPRC) was proposed and examined to recover P from municipal whole digestate via simultaneous leaching and precipitation. The anode of the EPRC released P as aqueous PO43--P through acidification, achieving the highest leaching efficiency of 93.3% under a current density of 30 A m-2. When the leached P solution was treated in the cathode, native metals including Ca and Fe facilitated electrochemically mediated PO43--P precipitation (EMP) and precipitated ∼99% of the leached P in the cathode chamber. Around 54.3-78.7% of total P existed in two harvestable forms: suspended solids in the cathode effluent and immobilized P in the cathode chamber. The solid products contained 28.42-33.51% of P2O5, comparable to the high-grade phosphate rock. Higher current densities reduced cathode scaling and resulted in a lower content of heavy metals in the solid products. An acidic solution was reused three times and effectively maintained cathode performance during a 42-cycle operation, achieving a consistent P recovery efficiency of nearly 80%. Those results have demonstrated the feasibility of the EPRC for recovering P from P-rich solid wastes.

Impacts of digestate-based compost on soil property and nutrient availability.

The treatment of digestate from food waste (DFW) has emerged as the bottleneck for food waste anaerobic digestion. DFW generally contains abundant nutrients that can be recycled by composting. However, the effect of DFW-based compost on soil improvement has not been extensively explored. In this study, soil properties were improved by adding various amounts of DFW-based compost, and the growth conditions of Pak choi were monitored. The results indicated that the DFW-based compost could provide nitrogen, calcium, magnesium, and organic matter, thereby enhancing the growth of Pak choi, accumulating chlorophyll, and improving photosynthesis efficiency. As the amount of added DFW-based compost increased from 0% to 20%, the fresh biomass, leaf weight, and root weight of Pak choi increased by 242%, 262%, and 99%, respectively. The total chlorophyll content was 2.62 mg g-1 in control and increased to 12.45 mg g-1 in the group with 20% DFW-based compost, benefiting the photochemical efficiency of Pak choi. However, the growth was inhibited when the addition amount exceeded 20%, potentially due to excessive nutrient supplementation. Overall, the addition of 20% of DFW-based compost was suggested to promote the growth of Pak choi by providing proper nutrients.

Current and prognostic overview on the strategic exploitation of anaerobic digestion and digestate: A review.

The depletion of fossil fuels and increasing demand for energy are encountered by generating renewable biogas. Anaerobic digestion (AD) produces not only biogas, also other value-added products from the digestate using various organic, municipal and industrial wastes which have several benefits like remediating waste, reduces greenhouse gas emissions, renewable energy generation and securing socio-economic status of bio-based industries. This review work critically analyzes the biorefinery approaches on AD process for the production of biogas and digestate, and their direct and indirect utilization. The left-out residue obtained from AD is called 'digestate' which enriched with organic matter, nitrogen, heavy metals and other valuable micronutrients. However, the direct disposal of digestate to the land as fertilizer/landfills creates various environmental issues. Keeping this view, the digestate should be upgraded or transformed into high valued products such as biofertilizer, pyrochar, biodiesel, syngas and soil conditioner that can aid to enrich the soil nutrients and ensures the safe environment as well. In this context, the present review focused to illustrate the current techniques and different strategic exploitations on AD proper management of digestate products for storage and further applications. Such a technology transfer provides a proven strategic mechanism towards the enhancement of the sustainability of bio-based industries, attaining the energy demand, safest waste management, protection of environment and reduces the socio-economic issues of the industrial sector.

Effect of photocatalysis on the physicochemical properties of liquid digestate.

Antibiotic residues pose a risk to the agricultural application of liquid digestate. In our previous study, photocatalysis was employed to degrade the antibiotics in liquid digestate and observed that the removal efficiency of TC, OTC, and CTC was up to 94.99%, 88.92%, and 95.52%, respectively, at the optimal experimental level, demonstrating the feasibility of this technology. In this study, the liquid digestate after photocatalysis was analyzed to evaluate the effect of photocatalysis on the nutrients, phytotoxicity, and bacterial community of liquid digestate. The results showed that photocatalysis had little effect on the major nutrients TN, TP, and TK in liquid digestate. However, photocatalysis could cause an increase in tryptophan substances as well as soluble microbial by-products and a decrease in humic acid substances in the liquid digestate. The toxicity of liquid digestate after photocatalysis exhibited an increasing trend followed by a decreasing trend, and the liquid digestate after photocatalysis for 2 h had a promoting effect on seed germination and root growth. The richness, diversity, and evenness of bacterial communities in liquid digestate were decreased as a result of photocatalysis. The dominant species in the liquid digestate was dramatically changed by photocatalysis, and the antibiotic concentration also had a major effect on the dominant species in the liquid digestate after photocatalysis. After photocatalysis for 2 h, the dominant species in the liquid digestate changed from Firmicutes to Proteobacteria.

Removal of contaminants of emerging concern from the supernatant of anaerobically digested sludge by O3 and O3/H2O2: Ozone requirements, effects of the matrix, and toxicity.

Digestate is a rich source of nutrients that can be applied in agricultural fields as fertilizer or irrigation water. However, most of the research about application of digestate have focused on its agronomic properties and neglected the potential harm of the presence of contaminants of emerging concern (CECs). Aadvanced oxidation processes (AOPs) have proved to be effective for removing these compounds from drinking water, yet there are some constrains to treat wastewater and digestate mainly due to their complex matrix. In this study, the feasibility to remove different CECs from digestate using O3 and O3/H2O2 was assessed, and the general effect of the matrix in the oxidation was explained. While the lab-scale ozonation provided an ozone dose of 1.49 mg O3/mg DOC in 5 h treatment, almost all the compounds were removed at a lower ozone dose of maximum 0.48 mg O3/mg DOC; only ibuprofen required a higher dose of 1.1 mg O3/mg DOC to be oxidized. The digestate matrix slowed down the kinetic ozonation rate to approximately 1% compared to the removal rate in demineralized water. The combined treatment (O3/H2O2) showed the additional contribution of H2O2 by decreasing the ozone demand by 59-75% for all the compounds. The acute toxicity of the digestate, measured by the inhibition of Vibrio fisheries luminescence, decreased by 18.1% during 5 h ozonation, and by 34% during 5 h O3/H2O2 treatment. Despite the high ozone consumption, the ozone dose (mg O3/mg DOC) required to remove all CECs from digestate supernatant was in the range or lower than what has been reported for other (waste-)water matrix, implying that ozonation can be considered as a post-AD treatment to produce cleaner stream for agricultural purposes.

Effect of organic amendments obtained from different pretreatment technologies on soil microbial community.

The application of organic amendments (OAs) obtained from biological treatment technologies is a common agricultural practice to increase soil functionality and fertility. OAs and their respective pretreatment processes have been extensively studied. However, comparing the properties of OAs obtained from different pretreatment processes remains challenging. In most cases, the organic residues used to produce OAs exhibit intrinsic variability and differ in origin and composition. In addition, few studies have focused on comparing OAs from different pretreatment processes in the soil microbiome, and the extent to which OAs affect the soil microbial community remains unclear. This limits the design and implementation of effective pretreatments aimed at reusing organic residues and facilitating sustainable agricultural practices. In this study, we used the same model residues to produce OAs to enable meaningful comparisons among compost, digestate, and ferment. These three OAs contained different microbial communities. Compost had higher bacterial but lower fungal alpha diversity than ferment and digestate. Compost-associated microbes were more prevalent in the soil than ferment- and digestate-associated microbes. More than 80% of the bacterial ASVs and fungal OTUs from the compost were detected 3 months after incorporation into the soil. However, the addition of compost had less influence on the resulting soil microbial biomass and community composition than the addition of ferment or digestate. Specific native soil microbes, members from Chloroflexi, Acidobacteria, and Mortierellomycota, were absent after ferment and digestate application. The addition of OAs increased the soil pH, particularly in the compost-amended soil, whereas the addition of digestate enhanced the concentrations of dissolved organic carbon (DOC) and available nutrients (such as ammonium and potassium). These physicochemical variables were key factors that influenced soil microbial communities. This study furthers our understanding of the effective recycling of organic resources for the development of sustainable soils.

Nematode-Suppressive Potential of Digestates to Meloidogyne incognita and Heterodera schachtii.

Management of plant-parasitic nematodes uses host plant resistance, crop rotation, cultural methods, and nematicide applications. Host plant resistance is tedious to develop, and crop rotation and cultural methods are challenging to use. Environmental and human health concerns render sole reliance on chemical nematode suppression nonsustainable. Previously, digestate from anaerobically fermented maize silage suppressed Heterodera schachtii in Beta vulgaris crops. Here, seven digestates were investigated for nematode suppressive potential: liquid dairy manure digestate (LDMD), liquid dairy manure digestate with ammonia removed (LDMDA-), food waste digestate (FWD), liquid food waste digestate with ammonia removed (LFWDA-), liquid food waste digestate (LFWD), food waste hydrolysate from the Renewable Energy Anaerobic Digester (HREAD), and food waste hydrolysate from the South Area Transfer Station in Sacramento (HSATS). In a red radish (Raphanus sativus) bioassay with H. schachtii, digestates were amended at rates of 0.02, 0.11, 0.57, and 2.86 ml per 100 cm3 of soil. At a rate of 2.86 ml, all amendments except LDMDA- and LFWDA- significantly reduced juvenile root penetration compared with the infested control. In a greenhouse watermelon (Citrullus lanatus) bioassay with Meloidogyne incognita, amendments FWD, LFWD, HREAD, and HSATS as well as LDMD (less effectively) at 2.86 and 5.76 ml per 100 cm3 of soil significantly reduced egg masses per root system compared with the nontreated, nematode-infested control. In a microplot experiment with M. incognita and red radish, in the treatment amended with LFWD at 2.37 ml per 100 cm3 of soil, marketable yields were improved by approximately 50% over the nontreated control and were comparable with those in the treatment with the nematicide Reklemel. In a second microplot experiment with M. incognita and watermelon, treatments that contained LFWD at rates of 3.55 ml per 100 cm3 of soil had transient numerical effects of initial nematode suppression that were not maintained throughout the 3-month growth period. The results of these studies demonstrated that digestates FWD and LFWD consistently expressed some nematode-suppressive capacity.

The Use of Fungi of the Trichoderma Genus in Anaerobic Digestion: A Review.

Plant waste biomass is the most abundant renewable energy resource on Earth. The main problem with utilising this biomass in anaerobic digestion is the long and costly stage of degrading its complex structure into simple compounds. One of the promising solutions to this problem is the application of fungi of the Trichoderma genus, which show a high capacity to produce hydrolytic enzymes capable of degrading lignocellulosic biomass before anaerobic digestion. This article discusses the structure of plant waste biomass and the problems resulting from its structure in the digestion process. It presents the methods of pre-treatment of lignocellulose with a particular focus on biological solutions. Based on the latest research findings, key parameters related to the application of Trichoderma sp. as a pre-treatment method are discussed. In addition, the possibility of using the digestate from agricultural biogas plants as a carrier for the multiplication of the Trichoderma sp. fungi, which are widely used in many industries, is discussed.

Assessment of economic viability and production costs for the innovative microfiltered digestate.

This paper assesses the economic viability of the production process of an innovative form of digestate known as "microfiltered digestate", suitable for use as a fertilizer in driplines for permanent crops. A Break-Even Analysis was performed to determine the Break-Even Point which identifies the minimum quantity of products necessary to cover production costs at a certain selling price. Our results show that the production of microfiltered digestate provides positive economic viability for anaerobic digestion managers, providing them with an attractive market outlet and, at the same time, a new form of income. The experimental study was stated in Sicily, but it is replicable in any territorial context with anaerobic digestion plants. The results are in line with the principles of circular economy and are very current with reference to the use of organic fertilizers instead of chemical ones.