Phytochemical Screening and Evaluation of Antioxidant Properties and Antimicrobial Activity against Xanthomonas axonopodis of Euphorbia tirucalli Extracts in Binh Thuan Province, Vietnam.

Euphorbia tirucalli is a medicine plant possessing many bioactive properties. This paper focused on phytochemical screening (alkaloid, flavonoid, saponin, tannin, and anthraquinone), quantification of polyphenol and flavonoids, and activating evaluation of antioxidants and antimicrobial properties against Xanthomonas axonopodis of different extracts from Euphorbia tirucalli grown in Binh Thuan, Vietnam. The best activity fraction was used for purification and determining bioactive ingredients. The results showed that the phytochemical study revealed the presence of alkaloids, flavonoids, tannins, and terpenoids in the ethyl acetate fraction. Saponin and anthraquinone did not present in all extracts. The content of polyphenol and flavonoid of Euphorbia tirucalli stem was in the range of 16.65-106.32 mg EqAG/g and 97.97-450.83 µg QE/g. The ethyl acetate fraction showed higher amounts of polyphenol and flavonoids and antimicrobial activity against X. axonopodis than other fractions. The antioxidant (SC50) activity of Euphorbia tirucalli stem was in the range of 12.91 ± 0.70 and 528.33 ± 25.15 µg/mL. At concentrations of 5.0 and 7.5 mg/mL, the diameter of inhibition of the ethyl acetate fraction was 14.33 ± 0.76 mm and 17.87 ± 0.57 mm, respectively. The MIC (minimum inhibitory concentration) was 0.156 mg/mL. Scopoletin, gallic acid, and piperic acid got MICs corresponding to 78, 312, and 312 µg/mL, respectively. Scopoletin, gallic acid, and piperic acid were found in the ethyl acetate fraction of Euphorbia tirucalli and exhibited the treatment of citrus bacteria canker and plant diseases.

Chemical profile and cytotoxic evaluation of aerial parts of Euphorbia tirucalli L. on gastric adenocarcinoma (AGS cells).

Ethanol extract from the aerial parts of Euphorbia tirucalli L. as well as the latex of the plant suspended in water are used by the Brazilian population for the treatment of various diseases, including cancer. The purposes of this study were to determine if the ethanol extract is effective as cytotoxic agent against gastric adenocarcinoma cells (AGS) and its chemical composition by GC-MS, ESI-(-)-FT-ICR MS and (-)-ESI-LTQ-MS/MS. The results were compared with that of latex previously described by us. Hexane and aqueous fractions showed higher cytotoxicity on AGS cells. Nine triterpene compounds were detected by GC-MS in hexane fraction, including euphol and friedelin, while ellagic acid was identified as main phenolic compound in aqueous extract. Therefore, the greater cytotoxic activity of the ethanol extract of the aerial parts of Euphorbia tirucalli for gastric cancer, when compared to latex, seems to originate from the antiproliferative effects of ellagic acid and triterpenes.

Oil yield from Euphorbia tirucalli as an alternative bioenergy source in Tanzania.

Due to the worldwide increase in the demands for bioenergy sources to replace fossil fuels. Euphorbia tirucalli oil produced by stems and modified leaves (phylloclades) is a valuable liquid fuel as an alternative energy source for biofuel production. In this study, E. tirucalli oils were extracted from stem barks with varying stem girths (20cm-80cm) and fully grown apical phylloclades. Soxhlet apparatus was used in oil extraction according to Luque de Castro and García (2000). The percentage (%) oil yield was calculated by the weight of extracted oil to the total weight of the dried sample (20g). The study hypothesized that significantly higher oil yields would be obtained from larger stem girths than phylloclades of the same trees in different Agro-Ecological Zones (AEZs). Likewise, the difference in oil yields was higher in the Semi-arid AEZ than in the Southern highland and the Coast AEZs (p > 0.05). Results showed slightly higher oil yields from large stem girths [16.47% ± 0.34%/(20g) max ≡ 82.35% ± 0.34%/(100g) max] than phylloclades [15.7% ± 0.49%/(20g) max ≡ 78.5% ± 0.34%/(100gmax] in different AEZs. Oil yields from Semi-arid [82.35% ± 0.34%/(100g) max and 78.5% ± 0.49%/(100g) max] were slightly higher than Southern highland [79.0% ± 0.34%/(100g) max and 76.4% ± 0.49%/(100g) max] and the Coast [76.4% ± 0.34%/(100g) max and 70.25% ± 0.49%/(100g) max] AEZ. But the difference in oil yields was not significant (p > 0.05). Results exhibited increasing patterns in the percentage (%) oil yields from low (20cm) to larger (80cm) stem girths. High oil yields between stem girths (82.35%) and phylloclades (78.5%) suggest that E. tirucalli oil is suitable for liquid biofuel production as an energy source in Tanzania.