Fatty Acids from Different Fat Sources and Dietary Calcium Concentration Differentially Affect Fecal Soap Formation in Growing Pigs.

BACKGROUND: Calcium (Ca) can complex with free fatty acids in the gastrointestinal tract (GIT), leading to the formation of insoluble unabsorbable Ca-fatty acid soaps, contributing to the proposed effect of Ca on weight loss in humans. OBJECTIVES: We determined the effect of dietary Ca concentration and the individual long-chain fatty acids on Ca-fatty acid soap formation and fatty acid digestibility. METHODS: Nine-week-old crossbreed male pigs (n = 144; mean ± SD body weight: 21.7 ± 0.15 kg) were used as an animal model for digestion in the adult human. The animals received purified diets containing 4 Ca concentrations (0, 2, 4, and 6 g/kg diet) and 4 fat sources (tallow, palmolein oil, soybean oil, and olive oil) in a completely randomized design. Fatty acids, Ca, and Ca-fatty acid soaps were determined in feces (n = 9 per diet). RESULTS: Increasing dietary Ca led to a 4-fold increase (P ≤ 0.05) in excreted palmitic and stearic acid when diets contained tallow or palmolein oil as the major fat source. More than 80% of these excreted fatty acids were present as soaps. For the tallow-based diets, increasing dietary Ca led to a decrease in stearic acid digestibility from 91% to 66% (P ≤ 0.01) and in palmitic acid digestibility from 96% to 83% (P ≤ 0.01). For the olive oil- and soybean oil-based diets dietary Ca did not (P > 0.05) influence fatty acid excretion. CONCLUSIONS: Ca-fatty acid soap formation led to decreased fat absorption in the GIT of growing pigs, which supports the hypothesis that higher dietary Ca concentrations reduce fat absorption.

Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity.

Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from (14)C-labeled acetate to those supplied exogenously as (14)C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2-3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells.