Volume electron microscopy reconstruction uncovers a physical barrier that limits virus to phloem.

Most plant reoviruses are phloem-limited, but the mechanism has remained unknown for more than half a century. Southern rice black-streaked dwarf virus (Fijivirus, Reoviridae) causes phloem-derived tumors, where its virions, genomes, and proteins accumulate, and it was used as a model to explore how its host plant limits the virus within its phloem. High-throughput volume electron microscopy revealed that only sieve plate pores and flexible gateways rather than plasmodesmata had a sufficiently large size exclusion limit (SEL) to accommodate virions and potentially serve as pathways of virion movement. The large SEL gateways were enriched within the proliferated sieve element (SE) layers of tumors. The lack of such connections out of the SE-enriched regions of tumors defined a size-dependent physical barrier to high flux transportation of virions. A working model is proposed to demonstrate the mechanism underlying limitation of virus within phloem.

Transitions between smoking and vaping: Evidence (or lack thereof) on potential differences by gender and sex.

OBJECTIVE: To synthesize existing evidence on possible differential effects by sex and gender from two Cochrane reviews evaluating vaping and smoking transitions. METHODS: We screened included studies from two Cochrane reviews for studies reporting smoking outcomes based on gender or sex. The first review examines the effects of using e-cigarettes to help people quit smoking and includes randomized controlled trials and uncontrolled intervention studies published to July 2023. The second review aims to assess the evidence on the relationship between the use and availability of e-cigarettes and subsequent smoking in young people (aged 29 and younger) and includes quasi-experimental and cohort studies published to April 2023. Due to the paucity and heterogeneity of data, we report results narratively. RESULTS: 10 of 161 studies included in the two relevant reviews met our criteria. Only five reported analyzing whether observed effects or associations varied based on sex and/or gender. A further three provided relevant descriptive information, and two did not report overall outcomes regarding vaping and smoking transitions but did investigate whether these differed by sex/gender. Synthesized data were largely inconclusive, but there was some suggestion that vaping was more strongly associated with subsequent smoking in young males than females. No studies reported data on nonbinary participants. CONCLUSIONS: Despite plausible reasons why sex and gender may be moderators of vaping and smoking transitions, there is little evidence investigating this. Future studies of vaping and smoking transitions should conduct and report analyses investigating potential differences based on sex and gender.

Development of pENTR-NeCo-lacZα vectors for the preparation of negative control constructs in Gateway cloning.

Gateway cloning is a useful technology for the simple and reliable preparation of various construct in many organisms. However, there is a problem regarding the negative control construct in the Gateway cloning system. In this study, we developed the pENTR-NeCo-lacZα vector system to create an empty vector that can be used as a negative control construct in Gateway cloning.

Exploring the opinions and potential impact of unflavoured e-liquid on smoking cessation among people who smoke and smoking relapse among people who previously smoked and now use e-cigarettes: findings from a UK-based mixed methods study.

BACKGROUND: Although electronic cigarettes (e-cigarettes) appear to be effective in helping people who smoke to stop smoking, concerns about use of e-cigarettes among young people have led to restrictions on non-tobacco flavoured e-liquids in some countries and some US states. These restrictions could reduce the appeal of these products to non-smoking youth but could have negative consequences for people who smoke or use e-cigarettes. METHODS: In this mixed methods study, we recruited UK adults who smoked or used to smoke and subsequently vaped to explore their opinions of unflavoured e-liquids and their beliefs about how they would be impacted by hypothetical e-liquid flavour restrictions. Participants trialled an unflavoured e-liquid instead of their usual nicotine product for four hours and completed a survey and an online interview. RESULTS: Using Interpretive Phenomenological Analysis and graphically presented data, we found differences in participants' opinions of unflavoured e-liquid. If only unflavoured, tobacco flavoured, and menthol flavoured e-liquids remained on the UK market, some people who smoke or vape may be unaffected, but some may relapse to smoking or continue smoking. Despite most wanting to prevent young people from initiating vaping, participants had varying opinions on whether flavour restrictions would be an effective method. CONCLUSIONS: The findings highlight that people who smoke and vape could be impacted by flavour restrictions in a range of ways, some of which could have a potential adverse impact on harm reduction efforts in the UK (e.g., by making smoking more appealing than vaping).

The "Gateway" hypothesis: evaluation of evidence and alternative explanations.

BACKGROUND: Electronic nicotine delivery systems (ENDS) offer a substantial harm reduction opportunity for adults who smoke and are unlikely to quit. However, a major concern about ENDS is their use by non-smoking youth, and particularly whether ENDS are acting as a "gateway" that leads youth to later start smoking cigarettes. However, evidence for the gateway hypothesis can be interpreted in alternative ways, e.g. that youth who have certain characteristics were already predisposed to use both ENDS and cigarettes ("common liability" explanation). AIMS: This commentary provides an evaluation of the gateway hypothesis that is accessible by a lay audience. This paper first reviews and evaluates the evidence interpreted as supporting the gateway hypothesis. Important alternative explanations (i.e., common liability) are discussed, as are different types of evidence (i.e., population-level trends) that can help differentiate between these competing explanations. OVERVIEW: Evidence for the gateway hypothesis is based on the finding that youth who use ENDS are more likely to also smoke cigarettes. However, this evidence suffers from an important flaw: these studies fail to fully account for some youths' pre-existing tendency to use products containing nicotine, and inappropriately interpret the results as ENDS use causing some youth to smoke. Common liability studies suggest that ENDS use does not, in and of itself, directly cause youth to later smoke cigarettes, beyond their pre-existing tendency to use products containing nicotine. Population-level trends show that youth cigarette smoking declined faster after ENDS use became common, which contradicts the central prediction of the gateway hypothesis (i.e. that youth smoking would be more common following ENDS uptake, than otherwise be expected). CONCLUSION: Evidence offered in support of the gateway hypothesis does not establish that ENDS use causes youth to also smoke cigarettes. Instead, this evidence is better interpreted as resulting from a common liability to use both ENDS and cigarettes. Population-level trends are inconsistent with the gateway hypothesis, and instead are consistent with (but do not prove) ENDS displacing cigarettes. Policies based on misinterpreting a causal gateway effect may be ineffective at best, and risk the negative unintended consequence of increased cigarette smoking.

Industrial Internet of Things Gateway with OPC UA Based on Sitara AM335X with ModbusE Acquisition Cycle Performance Analysis.

This article presents the hardware and software architectures used to implement the Modbus Extension (ModbusE) IIoT gateway, the performance of the acquisition cycle at the PRU real-time programmable core level, the acquisition cycle communication flow-dispatcher-OPC UA server (Linux)-OPC UA client (Windows) as well as the performance analysis of data communications between the IIoT ModbusE gateway and the OPC UA client (Windows). In order to be able to implement both the ModbusE acquisition cycle and the OPC UA server, the BeagleBone Black Board was chosen as the hardware platform. This board uses the Sitara AM335x processor (Texas Instruments (TI), Dallas, TX, USA) from Texas Instruments. Thus, the acquisition cycle was implemented on the PRU0 real-time core, and the OPC UA server, running under the Linux operating system, was implemented on the ARM Cortex A8 processor. From the analysis of the communication speed of the messages between the OPC UA client and the ModbusE servers, it was found that the ModbusE acquisition cycle speed was higher than the acquisition speed of the OPC UA client.

RADAR-IoT: An Open-Source, Interoperable, and Extensible IoT Gateway Framework for Health Research.

IoT sensors offer a wide range of sensing capabilities, many of which have potential health applications. Existing solutions for IoT in healthcare have notable limitations, such as closed-source, limited I/O protocols, limited cloud platform support, and missing specific functionality for health use cases. Developing an open-source internet of things (IoT) gateway solution that addresses these limitations and provides reliability, broad applicability, and utility is highly desirable. Combining a wide range of sensor data streams from IoT devices with ambulatory mHealth data would open up the potential to provide a detailed 360-degree view of the relationship between patient physiology, behavior, and environment. We have developed RADAR-IoT as an open-source IoT gateway framework, to harness this potential. It aims to connect multiple IoT devices at the edge, perform limited on-device data processing and analysis, and integrate with cloud-based mobile health platforms, such as RADAR-base, enabling real-time data processing. We also present a proof-of-concept data collection from this framework, using prototype hardware in two locations. The RADAR-IoT framework, combined with the RADAR-base mHealth platform, provides a comprehensive view of a user's health and environment by integrating static IoT sensors and wearable devices. Despite its current limitations, it offers a promising open-source solution for health research, with potential applications in managing infection control, monitoring chronic pulmonary disorders, and assisting patients with impaired motor control or cognitive ability.

Design and Enhancement of a Fog-Enabled Air Quality Monitoring and Prediction System: An Optimized Lightweight Deep Learning Model for a Smart Fog Environmental Gateway.

Effective air quality monitoring and forecasting are essential for safeguarding public health, protecting the environment, and promoting sustainable development in smart cities. Conventional systems are cloud-based, incur high costs, lack accurate Deep Learning (DL)models for multi-step forecasting, and fail to optimize DL models for fog nodes. To address these challenges, this paper proposes a Fog-enabled Air Quality Monitoring and Prediction (FAQMP) system by integrating the Internet of Things (IoT), Fog Computing (FC), Low-Power Wide-Area Networks (LPWANs), and Deep Learning (DL) for improved accuracy and efficiency in monitoring and forecasting air quality levels. The three-layered FAQMP system includes a low-cost Air Quality Monitoring (AQM) node transmitting data via LoRa to the Fog Computing layer and then the cloud layer for complex processing. The Smart Fog Environmental Gateway (SFEG) in the FC layer introduces efficient Fog Intelligence by employing an optimized lightweight DL-based Sequence-to-Sequence (Seq2Seq) Gated Recurrent Unit (GRU) attention model, enabling real-time processing, accurate forecasting, and timely warnings of dangerous AQI levels while optimizing fog resource usage. Initially, the Seq2Seq GRU Attention model, validated for multi-step forecasting, outperformed the state-of-the-art DL methods with an average RMSE of 5.5576, MAE of 3.4975, MAPE of 19.1991%, R2 of 0.6926, and Theil's U1 of 0.1325. This model is then made lightweight and optimized using post-training quantization (PTQ), specifically dynamic range quantization, which reduced the model size to less than a quarter of the original, improved execution time by 81.53% while maintaining forecast accuracy. This optimization enables efficient deployment on resource-constrained fog nodes like SFEG by balancing performance and computational efficiency, thereby enhancing the effectiveness of the FAQMP system through efficient Fog Intelligence. The FAQMP system, supported by the EnviroWeb application, provides real-time AQI updates, forecasts, and alerts, aiding the government in proactively addressing pollution concerns, maintaining air quality standards, and fostering a healthier and more sustainable environment.

Comparison of the safety and efficacy of Neuroform Atlas stent deployed via Gateway balloon catheter and microcatheter for the treatment of intracranial stenosis.

OBJECTIVE: This study aimed to compare the safety and efficacy of the Atlas stent released by the Gateway catheter and microcatheter in the treatment of intracranial stenosis (IS). METHODS: The primary efficacy and safety outcomes were the in-stent restenosis (ISR) rate and post-procedural stroke or death within one month. RESULTS: Atlas stents were deployed using the Gateway catheter and microcatheter in 19 (57.6 %) and 14 (42.4 %) procedures, respectively. Follow-up imaging data were available for 26 patients; the incidence of ISR was 15.4 %, and the ISR rate was higher, though not significantly, in the microcatheter group than in the Gateway group (30.0% vs. 6.25 %, P = .39). Clinical follow-up data were available for 30 patients; the post-procedural stroke rate was 3.3 % within one month and 13.3 % from one month to one year. The post-procedural stroke rate within one month was higher, though not significantly, in the microcatheter group than in the Gateway group (7.7% vs. 0 %, P = .43). The Gateway group had a significantly lower rate of post-procedural stroke in the same territory than that of the microcatheter group (0% vs. 30.8 %, P = .026). A higher incidence of residual stenosis <30 % was found in the non-ISR group than in the ISR group (72.2% vs. 0 %, P = .014). CONCLUSIONS: This study provides preliminary evidence that the Atlas stent is safe and effective for IS treatment. The use of the Gateway catheter to deliver the Atlas stent appears to be safer than using microcatheter. The incidence of ISR may be related to the degree of the residual stenosis.

Beyond the US-SOMO-AF database: a new website for hydrodynamic, structural, and circular dichroism calculations on user-supplied structures.

At the 25th International Analytical Ultracentrifugation Workshop and Symposium, we described the recent implementation of the UltraScan SOlution MOdeler AlphaFold (US-SOMO-AF) database, containing hydrodynamic, structural, CD calculations, and other ancillary information, performed on the entire AF v2 database of predicted protein structures, containing more than 1,000,000 entries. The scope of the US-SOMO-AF database was that of providing direct access to pre-calculated physicochemical parameters for rapid assessment against their experimentally determined counterparts to test the compatibility in solution of predicted AlphaFold structures. In the meantime, the AlphaFold consortium has extended its database of predicted structures to an astonishing > 200 million entries, making it quite impractical for their coverage in the US-SOMO-AF database. Therefore, we have created the US-SOMO-Web site, allowing the rapid calculations of all the properties, as present in the US-SOMO-AF database, on user-supplied PDB and mmCIF structures, as well as allowing direct processing of the latest AlphaFold models. Major features on the website are described, along with current limitations and potential future developments.

Re: "Impact of the e-cigarette era on cigarette smoking among youth in the United States: A population-level study".

This is a letter to the editor of Preventive Medicine responding to Harrell et al.'s "Impact of the e-cigarette era on cigarette smoking among youth in the United States: A population-level study." (Harrell MB, Mantey DS, Baojiang C, Kelder SH, Barrington-Trimis J. Impact of the e-cigarette era on cigarette smoking among youth in the United States: A population-level study. Preventive Medicine 2022;164:107265).

Re: Impact of the "e-cigarette era" on cigarette smoking among youth: A population-level study.

Our original paper is: Harrell MB, Mantey DS, Baojiang C, Kelder SH, Barrington-Trimis J. Impact of the e-cigarette era on cigarette smoking among youth in the United States A population-level study. Preventive Medicine 2022; 164:107265). This is a response to correspondence received from Foxon and Juul Labs Inc. (JUUL) regarding our original paper.

Modified serial techniques "ASTRO" facilitated laparoscopic total mesorectal excision for ultralow-lying rectal cancer in obese male patients: how we do it (with video).

BACKGROUND: Laparoscopic total mesorectal excision (LaTME) is a technically challenging for ultralow-lying rectal cancer in obese male patients. Herein, we introduced modified serial techniques "ASTRO" to facilitate LaTME, and the short-term outcomes were presented. METHODS: A prospective study (NCT05067413) was conducted between December 2020 and January 2022. The modified serial surgical techniques "ASTRO" included 5 key steps: (1) Anterior peritoneal reflection (APR) dissection at the highest line along with a "n"-shaped membrane bridge; (2) suspending the APR with a purse-string suture through the bladder peritoneum to enlarge the operating space of the anterior rectal wall; (3) traction and counter-traction continuously of the rectum applied with a cotton tape around the rectum; (4) resection of the pelvic rectum on tripartition, followed by the sequence of "posterior > anterior > lateral" principle; and (5) the trans-anterior Obturator nerve gateway was adapted to transect the distal rectum. The operative data and postoperative short-term outcomes were collected. RESULTS: Twenty-four consecutive patients underwent this procedure successfully. The average body mass index (BMI) was 29.9±1.3. The average of tumor height from anal verge was 4.0 cm (range, 3.0-4.5 cm). The median operating time and blood loss was 217 min (range, 165-420 min) and 50 ml (range, 20-100 ml) respectively. The anterior operation space at the midsagittal plane of the pelvis inlet was increased by 2.0 ± 0.3 cm. The calculated dominant angle was 20 ± 3°. The length of stapling line was 6.8 ± 1.0 cm with 11 cases by one cartridge and 13 cases by 2 cartridges. Eight patients developed postoperative complications including 4 with anastomosis leakage (16.7%), 2 with urinary retention (8.3%), one with anastomotic stenosis (4.2%) and one with ileus (4.2%). All the complications were relatively mild and the patients recovered well. CONCLUSION: Modified serial techniques "ASTRO" could expand the operating space and facilitate LaTME in obese male patients, thereby reducing the risk of conversion to open and transanal dissection.

How do the post-graduation outcomes of students from gateway courses compare to those from standard entry medicine courses at the same medical schools?

BACKGROUND: Widening participation (WP) for underrepresented students through six-year gateway courses helps to widen the demographic representation of doctors in the UK. 'Most students from gateway courses graduate, even though many enter with lower grades than standard entry medicine students.' This study aims to compare the graduate outcomes of gateway and SEM cohorts from the same universities. METHODS: Data from 2007-13 from the UK Medical Education Database (UKMED) were available for graduates of gateway and SEM courses at three UK medical schools. Outcome measures were passing an entry exam on the first attempt, Annual Review of Competency Progression (ARCP) outcome and being offered a level one training position from the first application. The univariate analysis compared the two groups. Logistic regressions, predicting outcomes by course type, controlled for attainment on completion of medical school. RESULTS: Four thousand four hundred forty-five doctors were included in the analysis. There was no difference found in the ARCP outcome between gateway and SEM graduates. Gateway graduates were less likely to pass their first attempt at any membership exam than graduates of SEM courses (39% vs 63%). Gateway graduates were less likely to be offered a level 1 training position on their first application (75% vs 82%). Graduates of gateway courses were more likely to apply to General Practitioner (GP) training programmes than SEM graduates (56% vs 39%). CONCLUSIONS: Gateway courses increase the diversity of backgrounds represented within the profession and importantly the number of applications to GP training. However, differences in cohort performance are shown to continue to exist in the postgraduate arena and further research is required to ascertain the reasons for this.

Design and Experiment of Satellite-Terrestrial Integrated Gateway with Dynamic Traffic Steering Capabilities for Maritime Communication.

This study presents the architectural design and implementation of a multi-RAT gateway (MRGW) supporting dual satellite and terrestrial connectivity that enables moving maritime vessels, such as autonomous surface ships, to be connected to multiple radio access networks in the maritime communication environment. We developed an MRGW combining LTE and very-small-aperture terminal (VSAT) access networks to realize access traffic steering, switching, and splitting functionalities between them. In addition, we developed communication interfaces between the MRGW and end-devices connecting to their corresponding radio access networks, as well as between the MRGW and the digital bridge system of an autonomous surface ship, enabling the MRGW to collect wireless channel information from each RAT end-device and provide the collected data to the digital bridge system to determine the optimal navigation route for the autonomous surface ship. Experiments on the MRGW with LTE and VSAT end-devices are conducted at sea near Ulsan city and the Kumsan satellite service center in Korea. Through validation experiments on a real maritime communication testbed, we demonstrate the feasibility of future maritime communication technologies capable of providing the minimum performance necessary for autonomous surface ships or digitized aids to navigation (A to N) systems.

Data Interworking Model and Analysis for Harmonization of Smart Metering Protocols in IoT-Based AMI System.

In the energy sector, since the adoption of remote device management for massive advanced metering infrastructure (AMI) devices and Internet of Things (IoT) technology using a representational state transfer (RESTful) architecture, a blurred boundary has been developed between traditional AMI and IoT. With respect to smart meters, the standard-based smart metering protocol, called the device language message specification (DLMS) protocol, still has a predominant role in the AMI industry. Thus, we aim to propose a novel data interworking model in this article that embraces the DLMS protocol in AMI using the most promising IoT protocol, the so-called lightweight machine-to-machine (LwM2M) protocol. We provide a 1:1 conversion model using the correlation of the two protocols with an analysis of the object modeling and resource management methods of both the LwM2M and DLMS protocols. The proposed model utilizes a complete RESTful architecture, which is the most beneficial in the LwM2M protocol. It improves the average packet transmission efficiency and packet delay on the plaintext and encrypted text (session establishment and authenticated encryption) by 52.9%p and 9.9%p, respectively, and by 11.86 ms for both cases, compared to the encapsulation method of the LwM2M protocol, KEPCO's current approach. This work provides the key idea to unify the protocol for the remote metering and device management of field devices into the LwM2M protocol, and it is expected that this work will improve the efficiency in the operation and management of KEPCO's AMI system.

Beacon Success Rate versus Gateway Density in Sub-GHz Sensor Networks.

Multiple Gateways (GWs) provide network connectivity to Internet of Things (IoT) sensors in a Wide Area Network (WAN). The End Nodes (ENs) can connect to any GW by discovering and acquiring its periodic beacons. This provides GW diversity, improving coverage area. However, simultaneous periodic beacon transmissions among nearby GWs lead to interference and collisions. In this study, the impact of such intra-network interference is analyzed to determine the maximum number of GWs that can coexist. The paper presents a new collision model that considers the combined effects of the Medium Access Control (MAC) and Physical (PHY) layers. The model takes into account the partial overlap durations and relative power of all colliding events. It also illustrates the relationship between the collisions and the resulting packet loss rates. A performance evaluation is presented using a combination of analytical and simulation methods, with the former validating the simulation results. The system models are developed from experimental data obtained from field measurements. Numerical results are provided with Gaussian Frequency Shift Keying (GFSK) modulation. This paper provides guidance on selecting GFSK modulation parameters for low bit-rate and narrow-bandwidth IoT applications. The analysis and simulation results show that larger beacon intervals and frequency hopping help in reducing beacon loss rates, at the cost of larger beacon acquisition latency. On the flip side, the gateway discovery latency reduces with increasing GW density, thanks to an abundance of beacons.

Disrupted Neural Regeneration in Dry Eye Secondary to Ankylosing Spondylitis-With a Theoretical Link between Piezo2 Channelopathy and Gateway Reflex, WDR Neurons, and Flare-Ups.

This study aimed at analyzing the corneal neural regeneration in ankylosing spondylitis patients using in vivo corneal confocal microscopy in correlation with Langerhans cell density, morphology, and dry eye parameters. Approximately 24 ankylosing spondylitis subjects and 35 age- and gender-matched control subjects were enrolled. Data analysis showed that all corneal nerve-fiber descriptives were lower in the ankylosing spondylitis group, implicating disrupted neural regeneration. Peripheral Langerhans cell density showed a negative correlation with nerve fiber descriptions. A negative correlation between tear film break-up time and corneal nerve fiber total branch density was detected. The potential role of somatosensory terminal Piezo2 channelopathy in the pathogenesis of dry eye disease and ankylosing spondylitis is highlighted in our study, exposing the neuroimmunological link between these diseases. We hypothesized earlier that spinal neuroimmune-induced sensitization due to this somatosensory terminal primary damage could lead to Langerhans cell activation in the cornea, in association with downregulated Piezo1 channels on these cells. This activation could lead to a Th17/Treg imbalance in dry eye secondary to ankylosing spondylitis. Hence, the corneal Piezo2 channelopathy-induced impaired Piezo2-Piezo1 crosstalk could explain the disrupted neural regeneration. Moreover, the translation of our findings highlights the link between Piezo2 channelopathy-induced gateway to pathophysiology and the gateway reflex, not to mention the potential role of spinal wide dynamic range neurons in the evolution of neuropathic pain and the flare-ups in ankylosing spondylitis and dry eye disease.

How Media Reports on COVID-19 Conspiracy Theories Impact Consensus Beliefs and Protective Action: A Randomized Controlled Online Trial.

This study examines the influence of news coverage on coronavirus disease (COVID)-related conspiracy theories on consensus perceptions regarding the seriousness of COVID-19 and its impact on attitudes and behaviors. In an online experiment, 395 participants either watched a report containing conspiracy theories, scientific facts, or information about a political summit, and they subsequently completed a questionnaire. Viewing reports on conspiracy theories lead to higher assessments of consensus compared with other reports. Perceived consensus correlated positively with attitudes toward COVID, which further correlated positively with behavior. The study shows that news reports can bias assessments of consensus, which has implications for public communication.

Brick into the Gateway (BiG): A novel approach for faster cloning combining Golden Gate and Gateway methods.

Gateway system is one of the most known cloning systems, which makes it compatible with several expression vectors, including those used for Yeast Two-Hybrid (Y2H) and Bimolecular Fluorescence Complementation (BiFC) assays. However, this system is laborious and expensive due to its two-step cloning. In this research, we developed a new cloning strategy named Brick into the Gateway (BiG). This approach uses GoldenBraid/Gate assemblies to create a DNA fragment of interest flanked by attL sites, which can be directly recombined into Gateway destination vectors. BiG method showed a high recombination efficiency and ensured the correct reading frame, which was successfully tested in Y2H and BiFC assays. BiG has proven to be a rapid, low-cost, reusable, and directional cloning method which allows the merged use of systems.