Gurgem-7 toxicity assessment: Regulation of cell survival or death by traditional Mongolian prescription.

Surgical treatments and chemotherapy are the most commonly used methods of colorectal cancer treatment (CRC), unfortunately, these therapies have many side effects. Moreover, despite advances in primary and adjuvant treatments, the survival time in CRC patients is still unsatisfactory. Treatment options for patients with CRC continue to advance and recent research has shown that colorectal cancer is sensitive to plant-derived substances. The use of natural compounds contained in herbal extracts for the treatment of colon cancer or as adjunctive therapy for CRC gives patients a wide range of treatment options. In this study, we evaluate the potential toxicity of the Mongolian preparation - Gurgem-7 composed of Crocus sativus, Veronica officinalis, Capsella bursa-pastoris, Arctostaphylos uva-ursi, Calendula officinalis, Gentiana lutea, and Terminalia chebula. Therefore, the aim of this study was to determine its biological activities, biochemical and molecular features in vitro and composition analysis by HPLC-ESI-QTOF-MS/MS platform. We identified 18 metabolites and 8 of them were quantified. Majority of the secondary metabolites belonged to the group of phenolic constituents with taxifolin, chlorogenic acids' family, hydroxysafflor yellow A and hydroxybenzoic acid as leading compounds. In turn, our in vitro results suggest that the preparation inhibits cell metabolic activity through oxidative stress, numerous DNA damage and cell cycle arrest. Simultaneously enzymatic and non-enzymatic cell protection mechanisms mediated by TP53/Keap1 and Nrf2/HO-1 pathways may be activated in a cell-specific manner in vitro. In conclusion, we provide preliminary molecular evidence of the toxic properties of Gurgem-7 preparation to Caco-2 and CT26. WT cells related to insufficient action of their repair and adaptive mechanisms to stress conditions.

Module-hierarchical principle of compounding traditional multi2ingredient drugs / Монголын Анагаах Ухаан

It became clear from our latest research that the module- hierarchical principle is followed to compound the multi - ingredient drugs in traditional medicine. This time we have compared some pharmacological actions of the drugs: Gurgem-3, hepatic Gurgem-7, large in­testine Gurgem-7. Model index of drugs ability to reduce khii, shar, badgan was estimated by count method. Study was done on mice and rats using some general,special and elective pharmacological methods. Unnatural pathology such as hyperthermia, diarrhea, enterocolitis was conceived on animals afterwards recipe's action was studied . According to the research all these three drugs reduce fever, relieve pain and have actions against inflammation. But comparing the hepatic Gurgem-7 with the other drugs, it has tendency to precipitate the coagulation and in­crease the bilirubin, cholesterin and the bile acid secretion. Intestine Gurgem-7 has a tendency to prevent from and treat the gastrointestinal activity disorder more than oth­ers. Preconceptional investigation showed that the module - hierarchical principle of the compounding traditional drugs is proved to be a new scientifically well-founded method.