Toward Integrating EBPR and the Short-Cut Nitrogen Removal Process in a One-Stage System for Treating High-Strength Wastewater.

Enhanced biological phosphorus removal (EBPR) is an economical and sustainable process for phosphorus removal from wastewater. Despite the widespread application of EBPR for low-strength domestic wastewater treatment, limited investigations have been conducted to apply EBPR to the high-strength wastewaters, particularly, the integration of EBPR and the short-cut nitrogen removal process in the one-stage system remains challenging. Herein, we reported a novel proof-of-concept demonstration of integrating EBPR and nitritation (oxidation of ammonium to nitrite) in a one-stage sequencing batch reactor to achieve simultaneous high-strength phosphorus and short-cut nitrogen removal. Excellent EBPR performance of effluent 0.8 ± 1.0 mg P/L and >99% removal efficiency was achieved fed with synthetic high-strength phosphorus wastewater. Long-term sludge acclimation proved that the dominant polyphosphate accumulating organisms (PAOs), Candidatus Accumulibacter, could evolve to a specific subtype that can tolerate the nitrite inhibition as revealed by operational taxonomic unit (OTU)-based oligotyping analysis. The EBPR kinetic and stoichiometric evaluations combined with the amplicon sequencing proved that the Candidatus Competibacter, as the dominant glycogen accumulating organisms (GAOs), could well coexist with PAOs (15.3-24.9% and 14.2-33.1%, respectively) and did not deteriorate the EBPR performance. The nitrification activity assessment, amplicon sequencing, and functional-based gene marker quantification verified that the unexpected nitrite accumulation (10.7-21.0 mg N/L) in the high-strength EBPR system was likely caused by the nitritation process, in which the nitrite-oxidizing bacteria (NOB) were successfully out-selected (<0.1% relative abundance). We hypothesized that the introduction of the anaerobic phase with high VFA concentrations could be the potential selection force for achieving nitritation based on the literature review and our preliminary batch tests. This study sheds light on developing a new feasible technical route for integrating EBPR with short-cut nitrogen removal for efficient high-strength wastewater treatment.

Application of a membrane-less air cathode microbial fuel cell to treat municipal waste composting leachate.

The adverse effects of high strength wastewaters on the microbial activities have created a challenge to biological treatments. Microbial fuel cell has been considered as a promising process because the electrical potential generation can stimulate microorganisms and overcome the inhibitory effect. However, several issues (e.g., scalability, high costs and maintenance) have prevented the process from the industrial applications. Elimination of the proton exchange membrane has been suggested as a remedy to the mentioned problems. In this work, a membrane-less microbial fuel cell was modified by putting the cathode within a thin sand layer (instead of the proton exchange membrane) to treat a high strength wastewater sample. The influences of the feed organic load and time of treatment in the modified system were studied in batch and continuous operations. It was revealed that the batch operation efficiency was higher for the lower feed loadings as a 5-day batch treatment removed 66 ± 4% of the 15,000 ± 500 mg/L initial chemical oxygen demand while the continuous process efficiency with 9-day hydraulic residence time was slightly more than 50%. However, the efficiency of the continuous operation for treatment of higher initial loading values was better than the batch mode with the removal efficiency of 41 ± 2% versus 12 ± 2% for a more concentrated leachate feed (45,000 ± 1000 mg/L). Finally, it was disclosed that the modified membrane-less MFC employed in this work can be effective in treatment of high strength wastewaters in larger scales with lower costs.

High-strength anaerobic digestion wastewater treatment by aerobic granular sludge in a step-by-step strategy.

To reduce the instability of aerobic granular sludge (AGS) caused by high-strength anaerobic digestion wastewater, a strategy of increasing proportion of anaerobic digestion wastewater step-by-step was adopted in this study. High-performance stable AGSs were successfully cultivated with sequencing batch reactors by this strategy, which could efficiently treat high-strength anaerobic digestion wastewater with an influent chemical oxygen demand (COD) up to 5090 mg⋅L-1. After six phases of stepwise increasing COD loads, the sludge sizes increased from 0.5 mm to 1.5 mm, with the final mixed liquor suspended solids increased to 13,814 mg⋅L-1, and the final sludge volume index decreased to 15 mL⋅g-1. The extracellular polymeric substance (EPS), which is crucial to keep the stability of AGS, increased continuously from 85.1 mg⋅g-1 SS to 307.8 mg⋅g-1 SS with the increase of COD loads. Moreover, the removal efficiency of COD and TN could reach 92% and 87% for real high-strength anaerobic digestion wastewater treatment. The bacterial community analysis revealed that the family Rhodocyclaceae, Flavobacteriaceae, and Xanthomonadaceae were the major microbes of AGS, and were responsible for COD and TN removal, as well as EPS secretion. These findings may provide novel information and enrich AGS treatment of high-strength real wastewater.

Application of Natural Clinoptilolite for Ammonium Removal from Sludge Water.

Sludge water (SW) arising from the dewatering of anaerobic digested sludge causes high back loads of ammonium, leading to high stress (inhibition of the activity of microorganisms by an oversupply of nitrogen compounds (substrate inhibition)) for wastewater treatment plants (WWTP). On the other hand, ammonium is a valuable resource to substitute ammonia from the energy intensive Haber-Bosch process for fertilizer production. Within this work, it was investigated to what extent and under which conditions Carpathian clinoptilolite powder (CCP 20) can be used to remove ammonium from SW and to recover it. Two different SW, originating from municipal WWTPs were investigated (SW1: c0 = 967 mg/L NH4-N, municipal wastewater; SW2: c0 = 718-927 mg/L NH4-N, large industrial wastewater share). The highest loading was achieved at 307 K with 16.1 mg/g (SW1) and 15.3 mg/g (SW2) at 295 K. Kinetic studies with different specific dosages (0.05 gCLI/mgNH4-N), temperatures (283-307 K) and pre-loaded CCP 20 (0-11.4 mg/g) were conducted. At a higher temperature a higher load was achieved. Already after 30 min contact time, regardless of the sludge water, a high load up to 7.15 mg/g at 307 K was reached, achieving equilibrium after 120 min. Pre-loaded sorbent could be further loaded with ammonium when it was recontacted with the SW.

High-strength wastewater treatment using microbial biofilm reactor: a critical review.

Biofilm reactors retain microbial cells in the form of biofilm which is attached to free moving or fixed carrying materials, thus providing a high active biomass concentration and automatic liquid and solid separation. Nowadays, microbial biofilm reactors have been widely used in high-strength wastewater treatment where very high pollutant removal efficiency is required, which usually requires excessive space and aeration energy for conventional activated sludge-based treatment. This paper provides an overview of microbial biofilm reactors developed over the last half-century, including moving bed biofilm reactor (MBBR), trickling filter (TF) reactor, rotating biological contactor (RBC), membrane biofilm reactor (MBfR), passive aeration simultaneous nitrification and denitrification (PASND) biofilm reactor, for their applications in high-strength wastewater treatment of not only removing carbon, nitrogen, sulphur but also a variety of oxidized contaminants including perchlorate and bromate. Despite the advance of biofilm reactor that exhibits high resistance to excessive pollutants loading, its drawbacks both from engineering and microbiological point of view are reviewed. The future prospects of biofilm reactor are also discussed in this review paper.

High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater.

In this study, the microbial community structure was assessed in an anaerobic ammonium oxidation-upflow anaerobic sludge blanket (ANAMMOX-UASB) reactor treating high-strength wastewater (approximately 700 mg N L(-1) in total nitrogen) by employing Illumina high-throughput sequencing analysis. The reactor was started up and reached a steady state in 26 days by seeding mature ANAMMOX granules, and a high nitrogen removal rate (NRR) of 2.96 kg N m(-3) day(-1) was obtained at 13.2∼17.6 °C. Results revealed that the abundance of ANAMMOX bacteria increased during the operation, though it occupied a low proportion in the system. The phylum Planctomycetes was only 8.39 % on day 148 and Candidatus Brocadia was identified as the dominant ANAMMOX species with a percentage of 2.70 %. The phylum of Chloroflexi, Bacteroidetes, and Proteobacteria constituted a percentage up to 70 % in the community, of which the Chloroflexi and Bacteroidetes were likely to be related to the sludge granulation. In addition, it was found that heterotrophic denitrifying bacteria of Denitratisoma belonging to Proteobacteria phylum occupied a large proportion (22.1∼23.58 %), which was likely caused by the bacteria lysis and decay with the internal carbon source production. The SEM images also showed that plenty of other microorganisms existed in the ANAMMOX-UASB reactor.

Comparison of semi-batch vs. continuously fed anaerobic bioreactors for the treatment of a high-strength, solids-rich pumpkin-processing wastewater.

The objective of this work was to compare two different high-rate anaerobic bioreactor configurations--the anaerobic sequencing batch reactor (ASBR) and the upflow anaerobic solid removal (UASR) reactor--for the treatment of a solid-rich organic wastewater with a high strength. The two, 4.5-L reactors were operated in parallel for close to 100 days under mesophilic conditions (37°C) with non-granular biomass by feeding a pumpkin wastewater with ∼4% solids. The organic loading rate of pumpkin wastewater was increased periodically to a maximum of 8 g COD L(-1) d(-1) by shortening the hydraulic retention time to 5.3 days. Compositional analysis of pumpkin wastewater revealed deficiencies in the trace metal cobalt and alkalinity. With supplementation, the ASBR outperformed the UASR reactor with total chemical oxygen demand (COD) removal efficiencies of 64% and 53%, respectively, achieving a methane yield of 0.27 and 0.20 L CH4 g(-1) COD fed to the ASBR and UASR, respectively. The better performance realized with the ASBR and this specific wastewater was attributed to its semi-batch, dynamic operating conditions rather than the continuous operating conditions of the UASR reactor.

Economic evaluation of submerged anaerobic hybrid membrane bioreactor operating at mesophilic temperature.

A laboratory-scale mesophilic submerged anaerobic hybrid membrane bioreactor (An-HMBR) was operated for 270 days for the treatment of high-strength synthetic wastewater at different hydraulic retention times (HRTs) (3 days, 2 days, 1 day, and 0.5 days). Chemical oxygen demand (COD) removal efficiency of 92% was obtained with methane yield rate of 0.18 LCH4/g CODremoval at 1-day HRT. The results of lab scale reactor at 1-day HRT were utilized for upscaling and cost analysis. Cost analysis revealed that the total capital cost comprised tank system (48%), membrane cost (32%), screen and PUF sponge (5% each), PLCs (4%), liquid pumps (3%), and others (2%). The operational cost comprised chemical cost (46%), pumping energy (42%), and sludge disposal (12%). The results revealed that the tank and heating costs accounted for the largest fraction of the total life cycle cost for full-scale An-HMBR. The heating cost can be compensated by gas recovery. Sensitivity analysis revealed that the interest rates, influent flow, and membrane flux were the most crucial parameters which affected the total cost of An-HMBR.

Treatment capacity of a novel flexible fibre biofilm bioreactor treating high-strength milk processing wastewater.

This study was focused on the capacity investigation of a novel multistage flexible fibre biofilm reactor (MS-FFBR) to treat milk processing wastewater (MPW) with high organic loading (OLR). The MS-FFBR performance was evaluated at four intermediate stages separately, and also the final effluent quality of the overall system with an influent chemical oxygen demand (CODin) ranged from 1500 ± 20 to 6000 ± 50 mg/L and hydraulic retention times (HRTs) of 8, 12, and 16 h. By comparting the bioreactors into the four stages effectively enhanced the bioreactor's performance. The maximum TCOD removal efficiency was achieved at the first stage, which was about 89 ± 20, 82 ± 20, and 78 ± 20% at HRTs of 16, 12, 8 h, and low CODin of 1600 ± 20, 1590 ± 20, and 1673 ± 20 mg/L, respectively. However, the first stage had less contribution to TCOD removal at high CODin concentrations, reported to be about 42 ± 4%, 46 ± 4%, and 25 ± 4% at CODin of 5960 ± 40, 5830 ± 40, and 5870 ± 40 mg/L, respectively. Furthermore, the MS-FFBR was effective in removing total suspended solids (TSS) and turbidity. The bioreactor has reduced the effluent turbidity to 9.0 ± 0.2, 20.0 ± 0.6, and 16.1 ± 0.5 NTU at low CODin concentrations of 1600 ± 20, 1590 ± 20, and 1670 ± 20 mg/L and HRTs of 16, 12, and 8 h, respectively. The bioreactor revealed a high COD removal rate increased from 2.3 ± 0.1 to 12.2 ± 0.4 kg TCOD/m3d by increasing the OLR from 2.4 ± 0.1 to 17.6 ± 0.4 kg TCOD/m3d, confirming high reactor capacity for treatment of high-strength wastewater. Kinetic studies confirmed that the biomass yield was low at various HRTs ranging from 0.1 to 0.2 gVSS/gCOD.

Waste Activated Sludge-High Rate (WASHR) Treatment Process: A Novel, Economically Viable, and Environmentally Sustainable Method to Co-Treat High-Strength Wastewaters at Municipal Wastewater Treatment Plants.

High-strength wastewaters from a variety of sources, including the food industry, domestic septage, and landfill leachate, are often hauled to municipal wastewater treatment plants (WWTPs) for co-treatment. Due to their high organic loadings, these wastewaters can cause process upsets in both a WWTP's liquid and solids treatment trains and consume organic treatment capacity, leaving less capacity available to service customers in the catchment area. A novel pre-treatment method, the Waste Activated Sludge-High Rate (WASHR) process, is proposed to optimize the co-treatment of high-strength wastewaters. The WASHR process combines the contact stabilization and sequencing batch reactor processes. It utilizes waste activated sludge from a municipal WWTP as its biomass source, allowing for a rapid start-up. Bench-scale treatment trials of winery wastewater confirm the WASHR process can reduce loadings on the downstream WWTP's liquid and solids treatment trains. A case study approach is used to confirm the economic viability and environmental sustainability of the WASHR process compared to direct co-treatment, using life-cycle cost analyses and greenhouse gas emissions estimates.

Bioaugmentation with Tetrasphaera to improve biological phosphorus removal from anaerobic digestate of swine wastewater.

Tetrasphaera-enhanced biological phosphorus removal (T-EBPR) was developed by augmenting conventional EBPR (C-EBPR) with Tetrasphaera to improve phosphorus removal from anaerobic digestate of swine wastewater. At influent total phosphorus (TP) concentrations of 45-55 mg/L, T-EBPR achieved effluent TP concentration of 4.17 ± 1.02 mg/L, 54 % lower than that in C-EBPR (8.98 ± 0.76 mg/L). The enhanced phosphorous removal was presumably due to the synergistic effect of Candidatus Accumulibacter and Tetrasphaera occupying different ecological niches. Bioaugmentation with Tetrasphaera promoted the polyphosphate accumulation metabolism depending more on the glycolysis pathway, as evidenced by an increase in intracellular storage compounds of glycogen and polyhydroxyalkanoates by 0.87 and 0.34 mmol C/L, respectively. The enhanced intracellular storage capacity was coincidentally linked to the increase in phosphorus release and uptake rates by 1.23 and 1.01 times, respectively. These results suggest bioaugmentation with Tetrasphaera could be an efficient way for improved phosphorus removal from high-strength wastewater.

Landfill leachate biological treatment: perspective for the aerobic granular sludge technology.

Landfill leachates are high-strength complex mixtures containing dissolved organic matter, ammonia, heavy metals, and sulfur species, among others. The problem of leachate treatment has subsisted for some time, but an efficient and cost-effective universal solution capable of ensuring environmental resources protection has not been found. Aerobic granular sludge (AGS) has been considered a promising technology for biological wastewater treatment in recent years. Granules' layered structure, with an aerobic outer layer and an anaerobic/anoxic core, enables the presence of diverse microbial populations without the need for support media, allowing simultaneous removal of different pollutants in a single unit. Besides, its strong and compact arrangement provides higher tolerance to toxic pollutants and the ability to withstand large load fluctuations. Furthermore, its good that settling properties allow high biomass retention and better sludge separation. Nevertheless, AGS-related research has focused on carbon-nitrogen-phosphorus removal, mainly from sanitary sewage. This review aims to summarize and analyze the main findings and problems reported in the literature regarding AGS application to landfill leachate treatment and identify the knowledge gaps for future applications.

Partial Nitrification and Enhanced Biological Phosphorus Removal in a Sequencing Batch Reactor Treating High-Strength Wastewater.

Complex and high levels of various pollutants in high-strength wastewaters hinder efficient and stable biological nutrient removal. In this study, the changes in pollutant removal performance and microbial community structure in a laboratory-scale anaerobic/aerobic sequencing batch reactor (SBR) treating simulated pre-fermented high-strength wastewater were investigated under different influent loading conditions. The results showed that when the influent chemical oxygen demand (COD), total nitrogen (TN), and orthophosphate (PO43--P) concentrations in the SBR increased to 983, 56, and 20 mg/L, respectively, the COD removal efficiency was maintained above 85%, the TN removal efficiency was 64.5%, and the PO43--P removal efficiency increased from 78.3% to 97.5%. Partial nitrification with simultaneous accumulation of ammonia (NH4+-N) and nitrite (NO2--N) was observed, which may be related to the effect of high influent load on ammonia- and nitrite-oxidising bacteria. The biological phosphorus removal activity was higher when propionate was used as the carbon source instead of acetate. The relative abundance of glycogen accumulating organisms (GAOs) increased significantly with the increase in organic load, while Tetrasphaera was the consistently dominant polyphosphate accumulating organism (PAO) in the reactor. Under high organic loading conditions, there was no significant PAO-GAO competition in the reactor, thus the phosphorus removal performance was not affected.

Simultaneous methanogenesis and denitrification coupled with nitrifying biofilm for high-strength wastewater treatment: Performance and microbial mechanisms.

A combined system consisting of an upflow blanket filter (UBF) and a moving-bed biofilm reactor (MBBR) was developed for the simultaneous removal of organic matters and ammonia from high-strength wastewater. With a constant COD of approximately 2000 mg/L and ammonium nitrogen in a series of concentrations (e.g., 50, 200 and 400 mg/L in stages I to III) of the influent wastewater, the removal efficiencies of COD, ammonium nitrogen and total nitrogen reached 96.10%-98.19%, 100%, and 79.12%-82.15%, respectively. With the increase of influent ammonia nitrogen concentration, the specific methanogenic activity of the UBF granules decreased significantly, while the specific denitrification rates of the UBF granules and specific nitrification rates of the MBBR biofilms increased significantly. Microbial community analysis showed that Methanobacterium and Methanosaeta were the dominant methanogens in the UBF granules, while Candidatus Competibacter, Thauera and Acinetobacter were identified as dominant denitrifiers. In addition, nitrifiers were enriched in MBBR biofilms at 11.33% and 13.87% of the average abundance of Nitrosomonas and Nitrospira, respectively, at stage III (influent ammonium at 400 mg/L, COD/NH4+-N =  5). The ecological network analysis, including full-networks and sub-networks, indicated that the interactions between methanogens and denitrifiers in the UBF granules were strong when the influent ammonium concentration reached 400 mg/L. No intensive interactions were observed among the functional bacteria in the MBBR biofilms over the entire operation. Overall, this study provides a new strategy for the application and construction of efficient biological processes to achieve simultaneous removal of organic matter and nitrogen for high-strength wastewater treatment.

Arrested methanogenesis digestion of high-strength cheese whey and brewery wastewater with carboxylic acid production.

A new anaerobic digestion process based on arrested methanogenesis (AM) was developed to treat high-strength cheese whey and brewery wastewater with simultaneous carboxylic acid production. This study specifically determined the links between wastewater characteristics, microbial community structure, and the operation of AM digesters at the bench scale. The highest total carboxylic acids concentration (78 g/L) was achieved after 15 days under batch condition at 40 °C and near-neutral pH. Lactate conversion to chain-elongated volatile fatty acid was observed. Under fed-batch conditions, the highest total acid productivity was 16 g/(Lliq·d) with substrate conversion of 0.66 g CODdigested/g CODfed at hydraulic residence time (HRT) of 4 days. Fed-batch digestion with biomass recycling resulted in a 2-fold increase in VFAs concentration (30 g/L) and a higher diversity in the microbial consortia. Experimental results show that highly efficient, robust, and productive community structure was established for sustainable carboxylate production from widely varying high-strength wastewaters.

Enzymatic pretreatment to enhance anaerobic bioconversion of high strength wastewater to biogas: A review.

Oil and grease, carbohydrate, protein, and lignin are the main constituents of high strength wastewaters such as dairy wastewater, cheese whey wastewater, distillery wastewater, pulp and paper mill wastewater, and slaughterhouse wastewaters. These constituents have contributed to various operational problems faced by the high-rate anaerobic bioreactor (HRAB). During the hydrolysis stage of anaerobic digestion (AD), these constituents can be hydrolyzed. Since hydrolysis is known to be the rate-limiting step of AD, the overall AD can be enhanced by improving the hydrolysis stage. This can be done by introducing pretreatment that targets the degradation of these constituents. This review mainly focuses on the biological pretreatment on various high-strength wastewaters by using different types of enzymes namely lipase, amylase, protease, and ligninolytic enzymes which are responsible for catalyzing the degradation of oil and grease, carbohydrate, protein, and lignin respectively. This review provides a summary of enzymatic systems involved in enhancing the hydrolysis stage and consequently improve biogas production. The results show that the use of enzymes improves the biogas production in the range of 7 to 76%. Though these improvements are highly dependent on the operating conditions of pretreatment and the types of substrates. Therefore, the critical parameters that would affect the effectiveness of pretreatment are also discussed. This review paper will serve as a useful piece of information to those industries that face difficulties in treating their high-strength wastewaters for the appropriate process, equipment selection, and design of an anaerobic enzymatic system. However, more intensive studies on the optimum operating conditions of pretreatment in a larger-scale and synergistic effects between enzymes are necessary to make the enzymatic pretreatment economically feasible.

Performance evaluation and substrate removal kinetics in an up-flow anaerobic hybrid membrane bioreactor treating simulated high-strength wastewater.

The prime objective of the present study is to evaluate the performance of novel up-flow anaerobic hybrid membrane bioreactor (An-HMBR) treating high-strength wastewater (synthetic) using polyurethane foam as filter media. Treatment efficiency of the entire An-HMBR varied from 88-97% corresponding to 0.67-3.90 d of hydraulic retention time (HRT) with organic loading rate of 6.4-1.06 kg COD m-3 d-1. The modified Stover-Kincannon model was the most appropriate model for An-HMBR and anaerobic hybrid bioreactor (excluding membrane). The suspended growth system in An-HMBR could be described by both modified Stover-Kincannon and Grau second order model. The attached growth system in An-HMBR followed conventional Monod's kinetics. A novel combination of suspended, attached and membrane in single reactor increased the solid retention time to as high as 756 d at 3.9 d HRT which not only improved the COD removal efficiency but also enhanced the performance of the membrane.

Coagulation of Chitin Production Wastewater from Shrimp Scraps with By-Product Chitosan and Chemical Coagulants.

Chitin production wastewater contains nutrient-rich organic and mineral contents. Coagulation of the wastewater with a natural coagulant such as by-product chitosan would be an economical and environmentally friendly method of treatment. This study investigated the treatment efficiencies of a preliminary sedimentation process followed by coagulation. The removal efficiencies for wastewater parameters were evaluated and compared for coagulants including by-product chitosan, polyaluminum chloride, and polyacryamide. The evaluation was based on the removal of wastewater turbidity and other criteria, including tCOD, sCOD, TKN, NH4+-N, TP, TSS, calcium, and crude protein. The results showed that the preliminary sedimentation (before coagulation) can remove over 80% of turbidity and more than 93% of TSS at pH 4 in 30 min. At optimal conditions, when the ratio of crude protein and calcium was 4.95, by-product chitosan dose of 77.5 mg·L-1 and pH = 8.3, the wastewater characteristics changes were tCOD 23%, sCOD 32%, TKN and ammonium 25%, TP 90%, TSS 84%, Ca2+ 29%, and crude protein 25%. The residue recovered through coagulation consists of up to 55 mg·g-1 crude protein, which is used for animal feed or crop fertilizer.

Performance of full-scale coagulation-flocculation/DAF as a pre-treatment technology for biodegradability enhancement of high strength wastepaper-recycling wastewater.

Paper mill wastewater leads to a serious damage on the environment owing to the high content of organic matters, suspended solids, toxic substances, and lignin. Hence, exploring new treatment technologies is the passion of environmental engineers to minimize the effluent impact on the environment and cleaner production aspects. This research focused on the performance of full-scale coagulation-fluctuation/DAF system for pre-treatment of high strength wastepaper-recycling wastewater and its effect on biodegradability improvement. To optimize unit performance, optimum coagulant and flocculant doses were firstly determined by jar test without prior change and adjustment of pH in lab-scale experiments. The optimum dosages were obtained by 1500 mg L-1 polyaluminum chloride (PACl) as coagulant coupled with 40 mg L-1 cationic polyacrylamide (C-PAM) as flocculant. Percentage removals of 39 ± 10, 6.8 ± 3.6, 31.0 ± 6.7, 24.0 ± 3.8, and 33.0 ± 20.0 were achieved for COD, sCOD, BOD5, sBOD5, and color, respectively. The average BOD5 to COD ratio after pre-treatment increased from 0.44 to 0.5, whereas the ratio of sBOD5/sCOD dropped from 0.65 to 0.53. A superior effectiveness in reduction of TSS (98.1%) and VSS (98.4%) was also achieved. The results show that the application process is able to prevent malfunction operation in the following bioreactors which is obtainable through bio-treatability enhancement of pre-treated wastewater and reducing the risks of clogging and sludge washout. Attached growth processes are suggested to be applied in the further anaerobic/aerobic processes because of high proportion of soluble fraction of COD in the effluent to avoid poor floc formation and dispersed growth problems.

Achieving an extraordinary high organic and hydraulic loadings with good performance via an alternative operation strategy in a multi-stage constructed wetland system.

In this study, a high organic loading rate of 58-146 g BOD5/m2 day with a hydraulic loading rate (HLR) of 1.63 m3/m2 day and retention time (RT) of 16 h was achieved to maximize the treatment capacity of a four-stage alum sludge-based constructed wetland (CW) system. An alternative operation strategy, i.e., the first stage anaerobic up-flow and the remaining stage tidal flow with effluent recirculation, was investigated to achieve the goal with good treatment performance of 82% COD, 91% BOD5, 92% SS, 94% NH4-N, and 82% TN removal. Two kinetic models, i.e., first-order model and Monod plus continuous stirred-tank reactor (CSTR) flow model, were employed for predicting the removal dynamics. The results showed that the tidal flow strategy enhances oxygen transport and diffusion, thus improving reduction of organics and NH4-N. Effluent recirculation could further increase elimination of organics by extending the interaction time and also benefit the denitrification process. In addition, denitrification could be further enhanced by anaerobic up-flow in the first stage.