Re-watering solution facilitates seed germination and seedling growth of Carex schmidtii: Implication for species re-introduction in degraded semi-arid wetlands.

Water deficiency threatens the health and function of wetlands in semi-arid areas. Optimum re-watering is an effective method for close-to-natural restoration to mitigate wetland degradation. Although the ecological importance of optimal re-watering as a nature-based solution for promoting wetland plant growth has been widely recognized, the response mechanisms of seed germination and seedling growth to re-watering are still poorly understood despite their decisive impact on plant life history. To fill this gap, this study compared the characteristics of seed germination and seedling growth in Carex schmidtii under initial water content with three levels (30%, 50%, and 70%) and five re-watering treatments (maintained at constant water content and re-watering to 100% on 7th, 14th, 21st, and 28th day). Moreover, the degree of reserve mobilization during four germination stages (seed suckering, sprouting, 20% germination, and seedling growth) was investigated. The results showed that water deficiency and re-watering treatments significantly affected C. schmidtii seed germination, seedling growth, and reserve mobilization. Compared with the other treatments, 50% moisture content and re-watering to 100% on the 14th day (50%-RT3) treatment significantly improved germination traits (germination rate, daily germination rate, germination index, and vigor index) and seedling growth characteristics (shoot length, root length, shoot biomass, root biomass, and total biomass). Furthermore, the degree of mobilization of starch, soluble protein, fat, and soluble sugar accumulation in C. schmidtii seeds under 50%-RT3 was higher than that in the other treatments. The structural equation model showed that the characteristics of seed germination and seedling growth of C. schmidtii were directly related to water deficiency and re-watering treatments, whereas reserve mobilization indirectly affected seed germination and seedling growth. These findings demonstrated that water deficiency and re-watering treatments have a crucial regulatory effect on seed germination and seedling growth of wetland plant species through a dual mechanism. This study provides information for the formulation of an optimum re-watering strategy for wetland vegetation restoration in semi-arid areas of the world.

Unraveling the role of anthropogenic and hydrologic drivers with respect to the optical and molecular properties of dissolved organic matter and organic phosphorus in a P-contaminated river.

Anthropogenic and hydrological drivers are key factors influencing the fate of dissolved organic matter (DOM) and dissolved organic phosphorus (DOP) in river runoff. However, how anthropogenic disturbances and hydrological conditions jointly affect the composition and characteristics of DOM and DOP in river runoff remains unclear. This study used fluorescence spectroscopy, Fourier transform ion cyclotron resonance mass spectrometry, and the stable water isotopes to interpret the chemical composition and properties of DOM and DOP as well as their linkages to anthropogenic disturbances and hydrological conditions in a typical P-contaminated tributary to the central Yangtze River. The results show in the wet season, the average abundance of humic-like components in DOM exceeded 60 %, while the average abundance of tryptophan-like components in DOM exceeded 50 % in the dry season. During the dry season, hydrological conditions had a greater impact on highly unsaturated DOM compounds compared to anthropogenic disturbances because a decrease in precipitation reduced the transport of terrestrial DOM into aquatic systems and increased water retention time in the river, promoting the production of unsaturated compounds from photochemistry. The effects of the two factors were similar in the wet season because active agricultural activities and intense precipitation jointly facilitated the entry of exogenous humics into the runoff, leading to the similar relative abundance of highly unsaturated DOM compounds associated with both factors. Anthropogenic disturbances had a greater impact on aliphatic DOM and DOP than hydrological conditions, which was associated with intense human activities in the watershed, such as phosphate mining, agricultural cultivation, and domestic sewage discharge. This study provides new knowledge about the composition, properties and underlying mechanisms of DOM and DOP in the P-contaminated watershed runoff.

Effect of site hydrological conditions and soil aggregate sizes on the stabilization of heavy metals (Cu, Ni, Pb, Zn) by biochar.

Biochar is a popular material that would effectively immobilize heavy metals in soil, which can greatly decrease the health risk of heavy metals. Although many previous studies have studied the immobilization of heavy metals by biochar, the influence of hydrological conditions on the immobilization effect is still not clear. This paper carried out column experiments to study the effect of fluctuating groundwater table on Cu, Ni, Pb, Zn distribution and speciation with the addition of biochar from pyrolysis of swine manure. Experimental results showed that biochar could significantly decrease the leaching toxicity of Cu and Ni by 24.4% and 44.7% respectively, while the immobilization effect of Pb and Zn was relatively insignificant. The average reduction percentage of bioavailable Cu was 14.5%, 39.5% and 33.3% in the unsaturated zone, fluctuating zone and saturated zone respectively, showing the better immobilization effect in the fluctuating zone and saturated zone. The residual fraction of heavy metals increased significantly after the addition of biochar, and the increase of residual fraction was larger in small soil aggregates. This study helped illustrate the influence of hydrological conditions and soil aggregate sizes on the stabilization effect of heavy metals by biochar, which could be used to guide the remediation process of sites contaminated by heavy metals.

High discharge intensified low net ecosystem productivity, hypoxia, and acidification at three outlets of the Pearl River Estuary, China.

Clarifying the influence of hydrological variations on ecological function is a topic of considerable interest in watershed ecological flow assessment and water resource management. Net ecosystem productivity (NEP) is a useful composite indicator of ecosystem function, reflecting material cycling and energy flow. However, the effects of hydrological variations on NEP, especially the influence mechanism, remain unclear due to the complex environmental characteristics in estuaries. We analysed the spatial-temporal variability of the aquatic environment and NEP through in-situ monitoring and field sampling from December 2018 to January 2020 at three outlets (Humen, Jiaomen, and Hongqimen) with different hydrological conditions in the Pearl River Estuary (PRE), China, and explored the influence mechanism of hydrological variation on NEP. The 155 groups of effective metabolism values were estimated using Odum's open-water method. The daily ecosystem respiration (ER) was higher than the gross primary production (GPP); therefore, water bodies were dominated by net heterotrophy at the three outlets. The daily NEP (-4.34 ± 1.40 mg O2 L-1d-1), O2 concentration (5.2 ± 1.02 mg L-1), and pH (7.53±0.24) were lowest at Humen, which also had the largest discharge and tide volume, deepest water depth, and widest channel. Seasonally, the NEP in the summer (-3.30 ± 1.39 mg O2 L-1d-1) and autumn (-3.19 ± 1.60 mg O2 L-1d-1) was lower than those in the spring (-1.56 ± 1.92 mg O2 L-1d-1) and winter (-2.17 ± 1.50 mg O2 L-1d-1). The inhibitory effect of increased discharge on the metabolic rate exceeded the stimulation provided by seasonal factors, such as increased temperature and solar radiation. The scour and dilution effect caused by discharge increase reduced chlorophyll a concentration; meanwhile, the increase in turbidity resulted in a decrease in the photosynthetic rate and GPP. ER was stimulated by heterotrophic microorganisms and high total suspended solids, resulting in a decrease in O2 and endogenous organics, thus causing the low NEP, hypoxia, and acidification phenomenon. Our results suggest that lengthening the discharge pulse period in summer and autumn will further decrease NEP and increase the area of hypoxia and acidification at the three outlets in the PRE.

Effects of hydrological change on the risk of riverine algal blooms: case study in the mid-downstream of the Han River in China.

Algal blooms usually occur in semi-closed water bodies such as lakes or estuaries; however, it has occurred frequently in the mid-downstream of the Han River (MSHR) in China since the 1990s. We made a comparative analysis of the hydrological conditions and identified the hydrological condition thresholds that induce algal blooms. From the hydrodynamic point of view, the changes and characteristics of the hydrological conditions in the MSHR were analyzed. Furthermore, the influence on the risk of algal blooms under different design water transfer schemes for the middle route of the South-to-North Water Diversion Project (SNWDP) was studied. The results indicated that (1) the flow in the MSHR less than 900 m3/s and water level in the Yangtze River higher than 14 m provided a suitable hydrological environment for diatoms multiply. (2) The flow of the MSHR showed a downtrend, while the water level of the Yangtze River showed an uptrend. There were variations in hydrological processes. Through specific IHA index analysis, the fact of flow reduction in the MSHR was demonstrated, and further indicated that algal bloom outbreak was in low flow period. (3) The water transfer in the middle route of SNWDP affected the risk probability of algal blooms. The more the amount of water transfer, the greater the risk probability of algal blooms. It was the Water Diversion Project from Yangtze River to Han River (WDPYHR) that replenished flow of the MSHR and was conducive to the prevention and control of algal bloom risk.

Seeking the hotspots of nitrogen removal: A comparison of sediment denitrification rate and denitrifier abundance among wetland types with different hydrological conditions.

Wetlands play a vital role in removing nitrogen (N) from aquatic environments via the denitrification process, which is regulated by multiple environmental and biological factors. Until now, the mechanisms by which environmental factors and microbial abundance regulate denitrification rates in wetlands under different hydrological conditions remain poorly understood. Here, we investigated sediment potential denitrification rate (PDR) and unamended denitrification rate (UDR), and quantified denitrifier abundance (nirS, nirK, and nosZ genes) in 36 stream, river, pond, and ditch wetland sites along the Dan River, a nitrogen-rich river in central China. The result indicated that ditches had the highest denitrification rates and denitrifier abundance. Both PDR and UDR showed strong seasonality, and were observed to be negatively correlated with water velocity in streams and rivers. Moreover, denitrification rates were significantly related to denitrifier abundance and many water quality parameters and sediment properties. Interestingly, PDR and UDR were generally positively associated with N and carbon (C) availability in streams and rivers, but such correlations were not found in ponds and ditches. Using a scaling analysis, we found that environmental parameters, including Reynolds number, sediment total C ratio, and interstitial space, coupled with relative nirS gene abundance could predict the hotspots of denitrification rates in wetlands with varying hydrologic regimes. Our findings highlight that hydrological conditions, especially water velocity and hydrologic pulsing, play a nonnegligible role in determining N biogeochemical processes in wetlands.

Influence of land use and rainfall on the optical properties of dissolved organic matter in a key drinking water reservoir in China.

The concentration, source and composition of dissolved organic matter (DOM) in aquatic ecosystems are associated with land use and hydrological connectivity between terrestrial and aquatic systems. However, direct evidence of the effects of rainfall and land use on the variability of DOM in aquatic ecosystems is very limited. In this study, chromophoric DOM (CDOM) absorption and fluorescence spectroscopy were used to elucidate how rainfall and land use affect the variability of CDOM in the watershed of Lake Tianmu, a key drinking water reservoir in the Yangtze River Delta. The mean values of the fluorescence intensity (Fmax) of parallel factor analysis-derived humic-like components (C1, C3, C6) and tryptophan-like components C5 were higher in the southeastern inflowing river mouths than those downstream of the lake outlet regions. The upstream tributaries were mainly dominated by humic-like materials, while the lake was mainly dominated by protein-like materials. The Fmax values of four humic-like components and two tryptophan-like components all increased significantly as the %woodland decreased, but %anthropogenic land use (%cropland+%urban construction area) increased. The Fmax of the humic-like components at the inflowing tributaries and the lake increased with increasing rainfall during storm events, and the value was especially pronounced at the inflowing river mouths. We concluded that land use and hydrological conditions play an important role in influencing the CDOM source and optical composition, and these findings provide insights for the understanding of aquatic ecosystem metabolism and reservoir water quality management.

Seasonal variability of groundwater level effects on the growth of Carex cinerascens in lake wetlands.

Groundwater level is crucial for wetland plant growth and reproduction, but the extent of its effect on plant growth can vary along with changed precipitation and temperature at different seasons. In this context, we investigated the effect of two groundwater levels (10 cm vs. 20 cm depth) on growth and reproductive parameters of Carex cinerascens, a dominant plant species in the Poyang Lake wetland, during three seasons (spring, summer, and autumn) and during two consecutive years (2015 and 2016). Carex cinerascens showed low stem number, height, and individual and population biomass in summer compared to spring and autumn. 10 cm groundwater level was overall more suitable for plant growth resulting in higher stem height and biomass. However, the interactive effect between groundwater level and season clearly demonstrated that the effect of groundwater level on plant growth occurred mainly in autumn. After the withering of the plant population in summer, we observed that C. cinerascens growth recovered in autumn to similar values observed in spring only with 10 cm groundwater level. Consequently, we could deduce that lowering groundwater level in the studied Poyang Lake wetland will negatively impact C. cinerascens regeneration and growth particularly during the second growth cycle occurring in autumn. Additionally, our results showed that, independently of the season and groundwater level, population biomass of C. cinerascens was lower during drier year. Altogether, our findings suggest that water limitation due to both reduction in precipitation and decreased groundwater level during the year can strongly impact plant communities.

Net primary productivity dynamics and associated hydrological driving factors in the floodplain wetland of China's largest freshwater lake.

Wetlands are thought to be the most unique ecosystem in the world which plays an important role in water and material circulation. However, investigation of ecosystem dynamics in those lake floodplain wetlands that suffering rapid and significant short-term water level fluctuation is quite a challenge. In this study, the short- and long-term characteristics of vegetation NPP (net primary productivity) and their driving mechanism were investigated in the Poyang Lake floodplain wetland, an important international wetland that listed in the Global Eco-region by the World Wildlife Fund (WWF). Attempts were achieved through validating the Carnegie-Ames-Stanford Approach (CASA) model based on observed biomasses of different vegetation types and reconstructed continuous high spatiotemporal resolution (30 m and 16 days) of NDVI data during 2000-2015 according to the fused Landsat and MODIS data. Major result indicates that the intra-annual variation of NPP of most vegetation types shows two peaks in a year due to combined effects of vegetation growth rhythm and seasonal exposure of the lake floodplain. Annual NPP of the lake floodplain ranges in 360.09-735.94 gC/m2 and shows an increasing trend during the study period. The change of NPP in space indicates that the distribution elevation of the major vegetation types decreased and evoluted toward the center lake floodplain. Different from the terrestrial ecosystem, inundation duration is the dominant factor that controls NPP dynamics in the lake floodplain, while the influences of other meteorological factors are much weakened. Recent decline of lake water level was the major reason for the spatio-temporal evolution of annual and seasonal vegetation NPP in the lake floodplain.

Long-term succession of aquatic plants reconstructed from palynological records in a shallow freshwater lake.

Aquatic plants in shallow freshwater lakes play a key role in stabilizing ecological function and providing valuable ecosystem services, yet they are severely degraded worldwide. An improved understanding of long-term aquatic plant succession is critical to investigate the potential driving mechanisms and to facilitate ecological restoration. In this paper, we reconstructed changes in the aquatic plant community over the past century based on palynological records from Changdang Lake, Middle and Lower Yangtze River Basin (MLYB), China. Our results reveal that aquatic plants in Changdang Lake have undergone three clear phases: emergent macrophytes dominated the aquatic vegetation in the 1900s-1970s, submerged macrophytes in the 1970s-1990s, and floating macrophytes increasingly after the 1990s. Significant changes in the aquatic plant communities were caused by increasing anthropogenic pressures, such as damming and nutrient loading from agriculture, aquaculture, and urbanization after the Chinese economic reform. We argue that Changdang Lake is currently in a transition phase between a macrophyte-dominated state and an algae-dominated state. Our palynological record is different from many contemporary studies, which suggest submerged plants dominated most lakes in this region before the 1950s. We suggest that the return of the aquatic plants to their 1970s-1980s state would be a realistic target for lake restoration. Our results show that palynological records can reveal long-term dynamics of macrophytes in shallow lakes for sustainable lake restoration and management.