Pollution characteristics and microbial community succession of a rural informal landfill in an arid climate.

Informal landfills pose potential threats to the environment and human health due to the lack of anti-seepage measures. However, little research has been conducted on the distribution of pollutants in informal landfill sites situated in arid climates, as well as the underlying interaction mechanisms between environmental factors and microbial structure. In this study, we sought to investigate the pollution characteristics and microbial community succession of the landfill in northern China. The results revealed that heavy metals in the landfill showed poor mobility and migration. The lower layers of the garbage samples had higher water-soluble contents of heavy metals compared to the upper layers. The landfill-derived dissolved organic matter (DOM) was found to originate from microbial production, and four fluorescent components were identified, including fulvic acid-like substances, humus-like substances, and protein-like components. Fluorescence intensity and humification degree increased with increasing depth. The microbial diversity and richness decreased with sampling depth. The most abundant phyla in the samples were Proteobacteria, unidentified_Bacteria, Bacteroidota, Firmicutes, Myxococcota, Gemmatimonadota, Actinobacteria, and Deinococcota. As the sampling depth increased, Proteobacteria decreased, while Bacteroidota and Firmicutes showed a remarkable increase, with little variation observed in the other phyla. The partial least-squares path model (PLS-PM) results indicated that pH had the most significant effect on microbial abundance and diversity (direct effect value = -5.560), while DOM and heavy metals had the opposite effect, with direct effects of 1.838 and 3.231, respectively. DOM was identified as the driving factor for the variation in other environmental factors. The redundancy analysis (RDA) showed that the dominant genera were greatly influenced by Cu, humic-like substances, and protein-like substances. Among them, Bacillus, Alcanivorax, Devosia, and Chryseolinea may play important roles in the remediation of landfills. Our study not only gains a deeper understanding of the pollution risk of informal landfills in arid climates, but also provides a scientific basis for the future treatment and restoration of contaminated sites associated with landfills.

Informal landfill contributes to the pollution of microplastics in the surrounding environment.

A large amount of plastic waste is generated yearly worldwide, and landfills are commonly used for the disposal of plastic waste. However, burying in landfill does not get rid of the plastic waste but leave the problem to the future. Previous works have showed that microplastics are presented in the landfill refuse and leachate, which might be potential sources of microplastics. In this work, characteristics of microplastic pollution in an informal landfill in South China were studied. Landfill refuse, underlying soil, leachate, and groundwater samples were collected from different sites within and around the landfill. Results show that microplastics in the landfill refuse and underlying soil varied from 590 to 103,080 items/kg and from 570 to 14,200 items/kg, respectively. Most of the microplastics are fibrous, small sized, and transparent. Polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET) are major polymer types. Scanning electron microscope (SEM) images and Fourier Transform Infrared (FTIR) spectra of the microplastic samples indicate varying degree of weathering. Microplastic abundances in the landfill leachate and groundwater ranged from 3 to 25 items/L and from 11 to 17 items/L, respectively. Microplastics detected in the landfill leachate and groundwater are even smaller compared with those in the refuse and underlying soil and their polymer types are more diverse. This work demonstrated that microplastics presented in an informal landfill without sufficient protection can leak out to the surrounding environment. The microplastic pollution originated from informal landfills should receive more attentions.

Diversity of microbial community structure and their association with phthalic acid esters and physicochemical parameters in informal landfills.

Informal landfill is a common waste treatment method employed in rural areas of China, and phthalic acid esters (PAEs) are one of the typical pollutants in landfill leachate. However, there is no corresponding theoretical basis for whether microbial treatment technology can be used to reduce environmental risk of PAEs in informal landfills. Thus, a typical informal landfill site in northern China was selected and approximately 1,133,023 effective sequences were obtained from 21 samples collected from three layers (different deposit depths) of the landfill. This research explored the correlation between PAEs and the composition and distribution of microbial community in specific environments of informal landfill sites. Here we found that dis(2-ethylhexyl) phthalate (DEHP), di-n-octyl phthalate (DOP), and diethyl phthalate (DEP) were positively and significantly correlated with Bhargavaea, Planococcus, Virgibacillus, and Oceanobacillus, respectively. The redundancy analysis demonstrated that moisture content, pH, NO2--N, and SUVA254 among the seven physicochemical factors (pH, TN, NO3--N,NO2--N,NH4+-N, SUVA254, and moisture content) significantly affected bacterial communities. The research conclusion can provide theoretical basis for the degradation technology of PAEs by microorganism and research basis for the treatment of informal landfill sites.

Microbial characteristics of the leachate contaminated soil of an informal landfill site.

Because informal landfills are not constructed in a regulated manner, they will inevitably become a source of leachate pollution to the surrounding environment over time. Microbes are an important part of the soil system, playing a vital role in maintaining the normal functionality of soil. This study investigated the microbial composition and co-occurrence pattern in the leachate contaminated soil of an informal landfill site. The landfill leachate underwent horizontal and vertical migration through the contaminated soil, resulting in significant differences in the microbial compositions of horizontal surface soil (CS) and vertical subsurface soil (DS and ES) compared to uncontaminated soil (S). The microbial diversity of CS, DS, and ES was lower than that of S. Due to the migration of landfill leachate, the microbial composition of the surface soil was substantially changed. The dominant phyla in S included Proteobacteria (26.88%), Chloroflexi (23.68%), Actinobacteroita (17.36%), and Acidobacteroita (16.86%), but in contaminated soils, Firmicutes (35.27-86.68%) were the dominant bacteria. A network analysis indicated that Bacilli, Clostridia, and Thermacetogeniazai of the Firmicutes were the keystone taxa and played a vital role in maintaining the stability of the soil ecosystem. A functional annotation of prokaryotic taxa (FAPROTAX) analysis showed that the microbes involved in the C-, N-, and S-cycles in contaminated soil were significantly different to those in uncontaminated soil. The proportion of (aerobic)-chemoheterotrophy and cellulolysis functional communities in contaminated soils was significantly reduced, while there was an increase in functional communities, such as anammox and denitrification, which are not conducive to soil nitrogen fixation. This negatively affected the maintenance of normal soil ecological functions. This study identified the microbial characteristics in leachate contaminated soil and the results will be beneficial for the remediation of contaminated soil in informal landfill sites.

Remediation Technology and Typical Case Analysis of Informal Landfills in Rainy Areas of Southern China.

A typical informal landfill in a rainy area of southern China was taken as an example in this study. The comprehensive control ideas and processes of the informal landfill site were systematically reviewed. The basic situation for the early stage of the government survey and investigation was provided, including a waste stock survey, water volume measurement, and a waste source survey. The main contents and key factors of a comprehensive investigation of the environmental quality status were briefly summarized. The water quality in the landfill, groundwater quality inside and outside of the site, and heavy metals in the bottom sediment were all determined. A low-cost practical landfill technology was explored to reduce the Chemical Oxygen Demand CODCr concentration of polyaluminum ferric chloride (PAFC), and NH4+-N was removed by calcium hypochlorite. Soil backfill was replaced, such that the informal landfill site was immobilized, which was perfectly suitable for this southern rainy area. This study proposes rules for a comprehensive improvement scheme for a landfill, and provides a reliable theoretical basis and practical experience for the treatment of similar informal landfills.