Characteristic and mechanism of biological nitrogen and phosphorus removal facilitated by biogenic manganese oxides (BioMnOx) at various concentrations of Mn(II).

Biogenic manganese oxides (BioMnOx) have attracted considerable attention as active oxidants, adsorbents, and catalysts. However, characteristics and mechanisms of nitrification-denitrification in biological redox reactions mediated by different concentrations of BioMnOx are still unclear. Fate of nutrients (e.g., NH4+-N, TP, NO3--N) and COD were investigated through different concentrations of BioMnOx produced by Mn(II) in the moving bed biofilm reactor (MBBR). 34% and 89.2%, 37.8% and 89.8%, 57.3% and 88.9%, and 62.1% and 90.4% of TN and COD by MBBR were synchronously removed in four phases, respectively. The result suggested that Mn(II) significantly improved the performance of simultaneous nitrification and denitrification (SND) and TP removal based on manganese (Mn) redox cycling. Characteristics of glutathione peroxidase (GSH-Px), reactive oxygen species (ROS), and electron transfer system activity (ETSA) were discussed, demonstrating that ROS accumulation reduced the ETSA and GSH-Px activities when Mn(II) concentration increased. Extracellular polymeric substance (EPS) function and metabolic pathway of Mn(II) were explored. Furthermore, effect of cellular components on denitrification was evaluated including BioMnOx performances, indicating that Mn(II) promoted the non-enzymatic action of cell fragments. Finally, mechanism of nitrification and denitrification, denitrifying phosphorus and Mn removal was further elucidated through X-ray photoelectron spectroscopy (XPS), high throughput sequencing, and fourier transform infrared reflection (FTIR). This results can bringing new vision for controlling nutrient pollution in redox process of Mn(II).

IoT Water Quality Monitoring and Control System in Moving Bed Biofilm Reactor to Reduce Total Ammonia Nitrogen.

Traditional aquaculture systems appear challenged by the high levels of total ammoniacal nitrogen (TAN) produced, which can harm aquatic life. As demand for global fish production continues to increase, farmers should adopt recirculating aquaculture systems (RAS) equipped with biofilters to improve the water quality of the culture. The biofilter plays a crucial role in ammonia removal. Therefore, a biofilter such as a moving bed biofilm reactor (MBBR) biofilter is usually used in the RAS to reduce ammonia. However, the disadvantage of biofilter operation is that it requires an automatic system with a water quality monitoring and control system to ensure optimal performance. Therefore, this study focuses on developing an Internet of Things (IoT) system to monitor and control water quality to achieve optimal biofilm performance in laboratory-scale MBBR. From 35 days into the experiment, water quality was maintained by an aerator's on/off control to provide oxygen levels suitable for the aquatic environment while monitoring the pH, temperature, and total dissolved solids (TDS). When the amount of dissolved oxygen (DO) in the MBBR was optimal, the highest TAN removal efficiency was 50%, with the biofilm thickness reaching 119.88 µm. The forthcoming applications of the IoT water quality monitoring and control system in MBBR enable farmers to set up a system in RAS that can perform real-time measurements, alerts, and adjustments of critical water quality parameters such as TAN levels.

Enhanced denitrification of the AO-MBBR system used for expressway service area sewage treatment: A new perspective on decentralized wastewater treatment.

Decentralized wastewater treatment warrants considerable development in numerous countries and regions. Owing to the unique characteristics of high ammonia nitrogen concentrations and low carbon/nitrogen ratio, nitrogen removal is a key challenge in treating expressway service area sewage. In this study, an anoxic/oxic-moving bed biofilm reactor (A/O-MBBR) and a traditional A/O bioreactor were continuously operated for 115 days and their outcomes were compared to investigate the enhancement effect of carriers on the total nitrogen removal (TN) for expressway service area sewage. Results revealed that A/O-MBBR required lower dissolved oxygen, exhibited higher tolerance toward harsh conditions, and demonstrated better shock load resistance than traditional A/O bioreactor. The TN removal load of A/O-MBBR reached 181.5 g‧N/(m3‧d), which was 15.24% higher than that of the A/O bioreactor. Furthermore, under load shock resistance, the TN removal load of A/O-MBBR still reached 327.0 g‧N/(m3‧d), with a TN removal efficiency of above 80%. Moreover, kinetics demonstrated that the denitrification rate of the A/O-MBBR was 121.9% higher than that of the A/O bioreactor, with the anoxic tank biofilm contributing 60.9% of the total denitrification rate. Community analysis results revealed that the genera OLB8, uncultured_f_Saprospiraceae and OLB12 were the dominant in biofilm loaded on carriers, and OLB8 was the key for enhanced denitrification. FAPROTAX and PICRUSt2 analyses confirmed that more bacteria associated with nitrogen metabolism were enriched by the A/O-MBBR carriers through full denitrification metabolic pathway and dissimilatory nitrate reduction pathway. This study offers a perspective into the development of cost-effective and high-efficiency treatment solutions for expressway service area sewage.

Bioaugmentation performance for moving bed biofilm reactor (MBBR) treating mariculture wastewater by an isolated novel halophilic heterotrophic nitrification aerobic denitrification (HNAD) strain (Zobellella B307).

Moving bed biofilm reactor (MBBR) demonstrates weak nitrogen removal for mariculture wastewater treatment under high salinity environment. An isolated novel halophilic heterotrophic nitrification aerobic denitrification (HNAD) strain (Zobellella B307) was applied in MBBR process to enhance nitrogen removal. Results showed that strain Zobellella B307 could remove 90.9% ammonia nitrogen (NH4+-N) and 97.1% nitrate nitrogen (NO3--N) after 10 h cultivation, and strong resistance to salinity variation (high growth and nitrogen removal efficiency with salinity of 65‰) was observed. Besides, the chemical oxygen demand (COD), NH4+-N and NO3--N removal reached 95.6%, 94.4% and 85.7% with the strain added into MBBR process. In addition, microbial community structure analysis reflected that the strain Zobellella B307 successfully proliferated (the relative abundance increased to 2.33%). The HNAD bacteria abundance increased and dominated during the nitrogen removal process with the strain inoculation. A microbial functional analysis revealed that the main dominant functional categories (carbohydrate metabolism and amino acid metabolism) increased with the bioaugmentation of strain Zobellella B307, thus improving the nitrogen removal.

Distribution and transformation of organophosphate esters in moving bed biofilm reactor.

A moving bed biofilm reactor (MBBR) process in wastewater treatment plants (WWTPs) uses plastic carriers, called biofilm carrier, to increase their treatment efficiency. Biofilm carrier is made up of plastic, containing the OPEs as flame retardants or plasticizers, so OPEs in biofilm carrier are possible to release from WWTPs to the river. This study investigated the effect of the MBBR process in WWTP on aquatic environments, focusing on OPEs. OPE eluted from the biofilm carrier by leaching test was tris(2-chloroethyl) phosphate (TCEP), and the concentration of the effluent compared to the influent was increased in the WWTP of the MBBR process. 3609 mg/day of TCEP would be discharged into the water using the second-order model with rate constant [Formula: see text] = 0.000451 (ng L-1)-1 h-1, which is the most suitable for the leaching concentration of TCEP. It was identified that TCEP in biofilm carrier was transformed into oxidative dechlorinated compounds and oxidative compounds by microorganisms in the bioreactor. As a result of the study, it was confirmed that not only TCEP but also transformation products of TCEP emitted into the water from the MBBR process of WWTP.

Time-lagged interspecies interactions prevail during biofilm development in moving bed biofilm reactor.

Clarifying the essential succession dynamics of interspecies interactions during biofilm development is crucial for the regulation and application of biofilm-based processes. In this study, regular and time-series phylogenetic molecular ecological networks were constructed to investigate ordinary and time-lagged interspecies interactions during biofilm development in a moving bed biofilm reactor. Positive interactions dominated both regular (89.78%) and time-series (77.04%) ecological networks, suggesting that extensive cooperative behaviors facilitated biofilm development. The pronounced directional interactions (72.52%) in the time-series network further indicated that time-lagged interspecies interactions prevailed in the biofilm development process. Specifically, the proportion of directional negative interactions was higher than that of positive interactions, implying that interspecific competition preferred to be time-lagged. The time-series network revealed that module hubs exhibited extensive time-lagged positive interactions with their neighbors, and most of them exhibited altruistic behaviors. Keystone species possessing more positive interactions were positively correlated with biofilm biomass, NO3 - -N concentrations, and the removal efficiencies of NH4 + -N and chemical oxygen demand. However, keystone species and peripherals that were negatively targeted by their neighbors showed positive correlations with the concentrations of NO2 - -N, polysaccharides, and proteins in the soluble microbial products. The data highlight that the time-series network can provide directional microbial interactions along with the biofilm development process, which would help to predict the tendency of community shifts and propose efficient strategies for the regulation of biofilm-based processes.

The role of immobilized quorum sensing strain in promoting biofilm formation of Moving Bed Biofilm Reactor during long-term stable operation.

Quorum sensing (QS) signaling plays a significant role in the natural regulation of biofilm formation. Multiple species QS systems in wastewater treatment processes have received significant attention in recent years and this study presents a long-term analysis of QS signaling, bacterial structures and extracellular polymeric substance (EPS) during biofilm formation, detachment and reformation processes. Six types of Acyl homoserine lactones (AHLs) were found to be closely related to different phases of biofilm development, with both QS and quorum quenching (QQ) strains being identified as drivers of various biofilm phases and 10 strains presenting a close relationship with AHLs (p < 0.05). Meanwhile, QS strain Sphingomonas rubra was immobilized and added into reactor systems, resulting in significant increase in AHL content, EPS production, and adhesion strength of biofilm (p < 0.05), which might promote biofilm formation processes during long-term stable operation. This study provides a potentially simple and economical way to improve activity and stability of MBBR in complex wastewater systems.

Composite hydrolytic acidification - aerobic MBBR process for treating traditional Chinese medicine wastewater.

Traditional Chinese medicine (TCM) wastewater is characterized by high organic content, unstable water quality and quantity and low biodegradability. In this paper, the hydrolytic acidification reactor-aerobic moving bed biofilm (MBBR) process was used to degrade TCM wastewater. Besides, a small pilot study was conducted. The appropriate operating parameters: hydraulic retention time (HRT) of the hydrolytic reactor was 16 h, HRT of MBBR was 30 h, dissolved oxygen of MBBR was 6 mg/L, sludge return ratio of MBBR was 100%. The hydrolytic reactor was started for 25 days. MBBR was run in series with the hydrolytic reactor after 24 days of separate operation. The start-up of the composite reactor was completed after another 26 days. The average removal efficiencies of chemical oxygen demand and ammonia nitrogen were 92% and 70%. The hydrolytic reactor was effective in decomposing macromolecules and MBBR had a strong ability to degrade pollutants through the excitation-emission-matrix spectra. The evolution pattern of the dominant bacterial genera and the surface morphology of sludge were studied by scanning electron microscopy and high-throughput sequencing analysis. It could be seen that the surface morphology of the biological filler was suitable for the growth and reproduction of microorganisms.

Characterizing and comparing microbial community and biofilm structure in three nitrifying moving bed biofilm reactors.

This study investigated biofilm establishment, biofilm structure, and microbial community composition of biofilms in three laboratory-scale moving bed biofilm reactors. These reactors were filled with three types of plastic carriers with varied depths of living space for microbial growth. The reactors were operated under the same influent and operational conditions. Along with the operation, the results showed that carriers with grids of 50 µm in height delayed the biofilm development and formed the thinnest biofilm and a carpet-like structure with the lowest α-diversity. In comparison, another two carriers with grids of 200 and 400 µm in height formed thick biofilms and large colonies with more voids and channels. Quantified properties of biofilm thickness, biomass, heterogeneity, portion of the biofilm exposed to the nutrient, and maximum diffusion distance were examined, and the results demonstrated that they almost (except for heterogeneity) strongly correlated to the α-diversity of microbial community. These illustrate that depth of living space, as an important parameter for carrier, could drive the formation of biofilm structure and community composition. It improves understanding of influencing factors on biofilm establishment, structure and its microbial community, and would be helpful for the design of biofilm processes.

Enhancement of MBBR reactor efficiency using effective microorganism for treatment of wastewater containing diazinon by engineered Pseudomonas putida KT2440 with manganese peroxidase 2 gene.

Pesticides not only are harmful to humans but they are noxious for water reservoirs, soil, and air quality as well. In this research, diazinon was removed from aqueous solutions by Moving Bed Biofilm Reactor (MBBR). The MBBR was spiked with transgenic Pseudomonas putida KT2440 with Pleurotus ostreatus fungus manganese peroxidase 2 gene to enhance the capabilities of Pseudomonas putida KT2440 in the degradation of diazinon. Although the amount of diazinon and COD and diazinon removal in the reactor including transgenic P. putida KT2440 was 95.46% and 97.47% and they were greater than the control and wild type (non-modified) P. putida KT2440 reactors, the surprising result was related to the adaptation pace of transgenic P. putida KT2440. The produced metabolites and the quantity of diazinon were assessed by HPLC and LC/MS. The metabolite hydroxyisopropyl diazinon was not found in the transgenic P. putida KT2440 reactor. Furthermore, a new sequence of cloned manganese peroxidase 2 gene has been recorded in GenBank with the accession number MT185558. According to bacterial identification of provided sludge the most frequent genus belonged to Aeromonas. Therefore, it seems that the MBBR in the presence of transgenic P. putida KT2440 with manganese peroxidase 2 gene can effectively remove the diazinon.

Influence of MBBR carrier geometrical properties and biofilm thickness restraint on biofilm properties, effluent particle size distribution, settling velocity distribution, and settling behaviour.

The relatively poor settling characteristics of particles produced in moving bed biofilm reactor (MBBR) outline the importance of developing a fundamental understanding of the characterization and settleability of MBBR-produced solids. The influence of carrier geometric properties and different levels of biofilm thickness on biofilm characteristics, solids production, particle size distribution (PSD), and particle settling velocity distribution (PSVD) is evaluated in this study. The analytical ViCAs method is applied to the MBBR effluent to assess the distribution of particle settling velocities. This method is combined with microscopy imaging to relate particle size distribution to settling velocity. Three conventionally loaded MBBR systems are studied at a similar loading rate of 6.0 g/(m2 •day) and with different carrier types. The AnoxK™ K5 carrier, a commonly used carrier, is compared to so-called thickness-restraint carriers, AnoxK™ Z-carriers that are newly designed carriers to limit the biofilm thickness. Moreover, two levels of biofilm thickness, 200 µm and 400 µm, are studied using AnoxK™ Z-200 and Z-400 carriers. Statistical analysis confirms that K5 carriers demonstrated a significantly different biofilm mass, thickness, and density, in addition to distinct trends in PSD and PSVD in comparison with Z-carriers. However, in comparison of thickness-restraint carriers, Z-200 carrier results did not vary significantly compared to the Z-400 carrier. The K5 carriers showed the lowest production of suspended solids (0.7 ± 0.3 g-TSS/day), thickest biofilm (281.1 ± 8.7 µm) and lowest biofilm density (65.0 ± 1.5 kg/m3). The K5 effluent solids also showed enhanced settling behaviour, consisting of larger particles with faster settling velocities.

Ecological Linkages between a Biofilm Ecosystem and Reactor Performance: The Specificity of Biofilm Development Phases.

In biofilm-based engineered ecosystems, the reactor performance was closely linked to interspecies interactions within a biofilm ecosystem, whereas the ecological processes underpinning such linkage were still unenlightened. Herein, the principles of community succession and assembly were integrated to capture the ecological laws of biofilm development by molecular ecological networks and assembly model analysis based on the 16S rRNA sequencing analysis and metagenomics in a well-controlled moving bed biofilm reactor. At the initial colonization phase (days 0-2, driven by initial colonizers), interspecific cooperation (74.18%) facilitated initial biofilm formation, whereas some pioneers, and keystone species disappeared at later phases. At the accumulation phase (days 3-30, rapid biofilm development), interspecific cooperation (81.41 ± 5.07%) contributed to rapid biofilm development and keystone species were mainly involved in quorum sensing or positively correlated with extracellular polymeric substance production. At the maturation phase (days 31-106, a well-adapted quasi-equilibrium state), increased interspecific competition (32.74 ± 4.77%) and higher small-world property facilitated the rapid information transportation and pollutant treatment, and keystone species were positively correlated with the removal of COD and NH4+-N. Homogenizing dispersal diminished the contemporary community dissimilarities, while turnover but rather nestedness governed the temporal variations in the biofilm succession period. This study highlighted the specificity of ecological processes at distinct biofilm development phases, which would advance our understanding on the development-to-function linkages in biofilm-based treatment processes.

Numerical study of hydrodynamic characteristics in a moving bed biofilm reactor.

The moving bed biofilm reactor (MBBR) has certain advantages, such as high wastewater treatment efficiency, low maintenance and operating costs, and simple operation. It has emerged as a valuable option for small decentralized facilities. The filling ratio, aeration mode and aeration intensity are the main factors that affect the performance of MBBRs in wastewater treatment. However, the information that concerns the used criteria that pertain to the process design for the MBBR is not adequate. In this study, a three dimensional computational fluid dynamics (CFD) model was constructed and the maximum error was only 1.98%, which was much smaller than the traditional 2D-CFD model. The filling ratio, aeration mode and aeration intensity of MBBR were optimized by CFD model from the point of view of fluid mechanics. The results show that the fluidization performance of the filling is the best under the one-side aeration mode with 30% filling ratio. The cost-performance ratio of the reactor with 30% filling ratio was 1.53, 25% and 35% filling ratio were only 1.17 and 1.14 respectively. Increasing the aeration intensity could improve the fluidization performance. However, the effect of high aeration intensity on the fluidization performance of the carrier was limited and the energy consumption increased greatly. The results revealed that when the aeration intensity increased from 0.07 min-1 to 0.13 min-1, the proportion of the carrier area increased by 16.56%. The proportion of the carrier area with an aeration rate of 0.20 min-1 was only 4.23%, which is higher than 0.13 min-1. The main factors that control the fluidization of the carrier were the range of the flow zone and the flow velocity of the liquid. Increasing the range of the flow zone could facilitate the flow of the carriers. The critical value of the flow velocity of the liquid in the flow zone was 0.04 m/s. These results could guide the optimization design of the filling ratio and the aeration conditions and provide a theoretical basis for the application of MBBR.

Simultaneous nitrification and denitrification in moving bed bioreactor and other biological systems.

Moving bed bioreactor (MBBR), used for treatment of municipal and industrial wastewater, is a completely mixed attached growth type system that involves microorganisms which grow as biofilm on the surface of the suspended carriers within the reactor. If the biofilm is thick enough, dissolved oxygen in the reactor would not diffuse into deeper strata and thus anoxic/anaerobic condition develops in those regions facilitating growth of heterotrophic denitrifying bacteria. Autotrophic nitrifiers colonize the outer surface of biofilm in biocarriers as usual. Thus, development of aerobic nitrifying and anoxic denitrifying microorganisms facilitates nitrification and denitrification simultaneously within different zones of the same biofilm. The present paper summarizes the feasibility of nitrogen removal in MBBR systems via autotrophic nitrification followed by heterotrophic denitrification, including various aspects of simultaneous nitrification and denitrification (SND) process in other biofilm units as well. Apart from that, the areas for further investigation are briefly narrated from studies conducted earlier.

Enhancement of mariculture wastewater treatment using moving bed biofilm reactors filled with modified biocarriers: Characterisation, process performance and microbial community evaluation.

This research investigated two proposed modified biofilm carriers' performances in treating recirculating aquaculture systems (RAS) wastewater under different salinities (12‰, 26‰, and 35‰) for about 92 days. Three moving bed biofilm reactors (MBBRs; R1, R2, and R3) were filled with unmodified novel sponge biocarriers (SB) served as a control, modified novel SB with ferrous oxalate (C2FeO4@SB), and modified novel SB with combined ferrous oxalate and activated carbon (C2FeO4-AC@SB), respectively. Under the highest saline condition, a significantly higher ammonia removal efficiency of 98.86 ± 0.7% (p ˃ 0.05) was obtained in R3, whereas R2 and R1 yielded 95.18 ± 2.8% and 91.66 ± 1.5%, respectively. Microbial analysis showed that Vibrio, Ruegeria, Formosa, Thalassospira, and Denitromonas were predominant genera, strictly halophilic heterotrophic nitrifying bacteria involved in nitrogen removal. In conclusion, the synergistic effects of novel sponge, C2FeO4 and AC accelerated biofilm formations and stability, subsequently enhanced the removal of ammonia from the mariculture RAS wastewater by the C2FeO4-AC@SB carriers in R3.

Moving bed biofilm reactor developed with special microbial seed for denitrification of high nitrate containing wastewater.

Biological denitrification is the most promising alternative approach for the removal of nitrate from wastewater. MBBR inoculated with activated sludge is a widely studied approach, but very few studies have focused on the bioaugmentation of biofilm forming bacteria in MBBR. Our study revealed that the use of special microbial seed of biofilm forming denitrifying bacteria Diaphorobacter sp. R4, Pannonibacter sp. V5, Thauera sp. V9, Pseudomonas sp.V11, and Thauera sp.V14 to form biofilm on carriers enhanced nitrate removal performance of developed MBBR. Various process parameters C/N ratio 0.3, HRT 3 h at Nitrate loading 2400 mg L-1, Filling ratio 20%, operated with Pall ring carrier were optimized to achieve highest nitrate removal. After 300 days of continuous operation results of whole genome metagenomic studies showed that Thauera spp. were the most dominant and key contributor to the denitrification of nitrate containing wastewater and the reactor was totally conditioned for denitrification. Overall, findings suggest that bench-scale MBBR developed with biofilm forming denitrifying microbial seed accelerated the denitrification process; therefore in conclusion it is suggested as one of the best suitable and effective approach for removal of nitrate from wastewater.

Combined effects of volume ratio and nitrate recycling ratio on nutrient removal, sludge characteristic and microbial evolution for DPR optimization.

The optimization of volume ratio (VAn/VA/VO) and nitrate recycling ratio (R) in a two-sludge denitrifying phosphorus removal (DPR) process of Anaerobic Anoxic Oxic-Moving Bed Biofilm Reactor (A2/O-MBBR) was investigated. The results showed that prolonged anaerobic retention time (HRTAn: 1.25→3.75 hr) exerted favorable effect on chemical oxygen demand (COD) removal (57.26%→73.54%), poly-ß-hydroxyalkanoates (PHA) synthesis (105.70→138.12 mgCOD/L) and PO43- release (22.3→38.9 mg/L). However, anoxic retention time (HRTA) and R exhibited positive correlation with PHA utilization (43.87%-81.34%) and denitrifying phosphorus removal (DPR) potential (ΔNO3-/ΔPO43-: 0.57-1.34 mg/mg), leading to dramatical TN removal variations from 68.86% to 81.28%. Under the VAn/VA/VO ratio of 2:6:0, sludge loss deteriorated nutrient removals but the sludge bioactivity quickly recovered when the oxic zone was recovered. The sludge characteristic and microstructure gradually transformed under the dissolved oxygen (DO) control (1.0-1.5→1.5-2.0 mg/L), in terms of sludge volume index (SVI: 194→57 mL/gVSS), median-particle-size (D50: 99.6→300.5 µm), extracellular polymeric substances (EPS) (105.62→226.18 mg/g VSS) and proteins/polysaccharides (PN/PS) ratio (1.52→3.46). Fluorescence in situ hybridization (FISH) results showed that phosphorus accumulation organisms (PAOs) (mainly Cluster I of Accumulibacter, contribution ratio: 91.79%-94.10%) dominated the superior DPR performance, while glycogen accumulating organisms (GAOs) (mainly Competibacter, contribution ratio: 82.61%-86.89%) was responsible for deteriorative TN and PO43- removals. The optimal HRTA and R assembled around 5-6.5 hr and 300%-400% based on the PHA utilization and DRP performance, and the oxic zones also contributed to PO43- removal although it showed low dependence on DO concentration and oxic retention time (HRTO).

Elucidating sludge characteristic, substrate transformation and microbial evolution in a two-sludge denitrifying phosphorus removal system under the impact of HRT.

Granule formation has been recognized as a promising biotechnology in denitrifying phosphorus removal (DPR) systems by facilitating phosphorus accumulation organisms (PAOs) especially denitrifying PAOs (DPAOs), and hydraulic selection made this a more difficult task in continuous operation. This study aimed at exploring the microscopic mechanism and putting forward an effective strategy for DPR granulation under the impact of hydraulic retention time (HRT) (12 h, 10 h, 8 h) in a novel Anaerobic Anoxic Oxic - Moving Bed Biofilm Reactor (A2/O - MBBR) system. With the reduction of intracellular carbon storage (CODintra) efficiency (88.58%-78.53%), nitrogen (N) (85.45%-79.11%) and phosphorus (P) (96.55%-92.47%) removals both dropped, but it exhibited a growth of anoxic phosphorus uptake rate (PURA) (3.79-5.68 mg P/(gMLVSS·h)). The batch tests associating with substrate transformation of poly-ß-hydroxyalkanoates (PHA), glycogen (Gly) agreed well with the corresponding stoichiometry of phosphorus release rate (PRR) (4.83-7.53 mg P/(gMLVSS·h)), PURA (3.55-5.43 mg P/(gMLVSS·h)), oxic phosphorus uptake rate (PURO) (6.08-6.21 mg P/(gMLVSS·h)), and DPAOs/PAOs ratios (57.17%-89.31%), indicating a shift of microbial community. DPR granules gradually stabilized with low sludge volume index (SVI5/SVI30 ratio = 1.1-1.2), dense and compact structure, higher P content (11.63%), more extracted extracellular polymeric substances (EPS) (111.40-160.31 mg/gMLVSS) as proteins/polysaccharides (PN/PS) ratios (1.70-3.47) increased, leading to better sludge settleability and cell hydrophobicity. Fluorescence in situ hybridization (FISH) results showed that PAOs (mainly Cluster I: 20.20%) were the dominant bacteria in the A2/O reactor although a small amount of Defluviicoccus (3.18-3.48%) was responsible for nitrite accumulation, while ammonium-oxidizing bacteria (AOB) (mainly Nitrosomonas: 10.75%) and nitrite-oxidizing bacteria (NOB) (mainly Nitrospira: 15.06%) were enriched in the MBBR.

Performance of novel sponge biocarrier in MBBR treating recirculating aquaculture systems wastewater: Microbial community and kinetic study.

In this study, a novel sponge biocarriers (SB) in moving bed bioreactor (MBBR) treating recirculating aquaculture systems wastewater was evaluated for the first time. Two lab-scale MBBRs were operated simultaneously for 116 days under various hydraulic retention times (HRTs). The reactors R1 and R2 were filled with K5 plastic carriers and SB, respectively. From the results, at an optimum HRT of 6 h, ammonia removal efficiency and nitrification rate were 86.67 ± 2.4% and 1.43 mg/L.h for the R1 and, 91.65 ± 1.3% and 1.52 mg/L.h for the R2, respectively. The microbial community analysis showed that the predominant genera in the nitrifying community were Nitrosomonas (AOB) and Nitrospira (NOB) in co-existence with heterotrophic genera Hyphomicrobium, Mesorhizobium, Zhizhongheella, and Klebsiella spp. Modified Stover-Kincannon model examined the ammonia removal kinetics, and the values of kinetic parameters obtained were Umax: 0.909 and 1.111 g/L.d and KB: 0.929 and, 1.108 g/L.d for the R1 and R2, respectively. The correlation coefficients (R2) of the MBBRs were higher than 0.98, indicating that the model adequately described the experimental data. Overall, MBBR, filled with the proposed novel SB operated at 6 h HRT, can achieve the highest nitrification performance and increase the diversity of the functional microbial communities.

Roles of nitrate recycling ratio in the A2/O - MBBR denitrifying phosphorus removal system for high-efficient wastewater treatment: Performance comparison, nutrient mechanism and potential evaluation.

The long-term effect of nitrate recycling ratios (R = 100%-500%) on the denitrifying phosphorus removal (DPR) characteristics was studied in a novel two-sludge system, which coupled Anaerobic Anoxic Oxic (A2/O) with Moving Bed Biofilm Reactor (MBBR) for simultaneous nitrogen (N) and phosphorus (P) removals. During the 220 days' operation, effluent COD (30.87-45.15 mg/L) can meet the discharge standard completely, but N and P removals were significantly affected by the R-value, including CODintra removal efficiency (CODintra-Re: 56.09-85.98%), TN removal (TN-Re: 52.06-80.50%), anaerobic PO43- release (PO43--An: 10.66-29.02 mg/L) and oxic PO43- absorption (PO43--O: 2.22-6.26 mg/L). Meanwhile, N and P displayed close correlation with the ΔPO43-/ΔNO3- ratio of 4.20-4.41 at R = 300%-400%, resulting in the high-efficient anoxic poly-ß-hydroxyalkanoates (PHA) utilization (ΔPHAA: 64.88 mgCOD/gVSS). Based on the stoichiometry methodology, at R of 300%-400%, the percentages of phosphorus accumulation organisms (PAOs) and glycogen accumulating organisms (GAOs) contributed to ΔPHAAn (ΔGlyAn) were 71.7%, 28.3% (61.3%, 38.7%) in the anaerobic stage, respectively, while N denitrification rate (NDRA: 3.91-3.93 mg N/(gVSS·h)) and P uptake rate (PURA: 3.76-3.90 mg P/(gVSS·h)) reached the peak, suggesting superior DPR performance with higher contribution of denitrifying PAOs (DPAOs) (70%) than denitrifying GAOs (DGAOs) (30%) in the anoxic stage. Microbial community analysis showed that Accumulibacter (27.66-30.01%) was more enriched than Competibacter (13.41-14.34%) and was responsible for the improved C, N, P removals and DPR characteristics. For optimizing operation, the combined effect of nitrate recycling ratio with other process parameters especially economic evaluation should be considered.